... repressor J Mol Biol 223, 54 5 55 5 39 Lim, F & Peabody, D.S (1994) Mutations that increase the affinity of a translational repressor for RNA Nucleic Acids Res 22, 3748–3 752 40 Lim, F., Spingola, ... (p.f.u.ÆmL)1) Control MS2 T45S M88V D11N 3.4 kbar of pressure 4 .5 M urea 9.0 M urea 108 108 108 108 108 108 107 108 1 05 102 102 107 ND ND ND 102 and T45S particles with 4 .5 M urea resulted in drastic ... measurements, the sample was excited at 320 nm and the emission measured from 3 15 to 3 25 nm Standard buffer: 50 mM Tris/ 150 mM NaCl (pH 7 .5) Fluorescence data points are the average and standard deviation...
... Regulation for Growth, Equity and Stability in the Post Crisis World” 15 16 November 2011, Mumbai Day – 15 November 2011 11. 45 12. 45 Inaugural session - Addresses by D Subbarao, Governor, RBI and Jaime ... cannot grow by leveraging But, we have now turned the whole finance theory on its head This is because all economic theory and finance theory start from the assumption of normality But we are not living ... Implications for Growth and Financial Sector Regulation Anand Sinha 45 Summary of the discussion 85 Financial Sector Regulation for Equity Chair’s initial remarks Stephany Griffith-Jones...
... bottom): 2, 5, 7, 10, 15, 20, 30, 45, 60, 75, 120, 150 and 180 lM The symbols in (B) correspond to various concentrations between and 180 lM at four different temperatures: 10, 15, 20 and 25 °C (h, ... conclusions Thus, for example, the DDSU, 25 values at 25 °C corresponding to those of DDGU, 25 in Table are )6.0, )5. 5 and )2.0 JÆK)1Æmol)1 at pH 4.00, 3. 75 and 3 .50 , respectively, and, given the known ... monomer concentration of 120 lM DGU, 25 and DDGU, 25 stand for the changes in standard Gibbs energy at 25 °C (DGU, 25) and their excess (DDGU, 25) above the DGU, 25 of the E34D mutant The Tg values...
... stability of HT c -55 2 (see below) For clarity, the residue numbers used for HT c -55 2 are those of PA c -55 1 A hyperthermophile, Aquifex aeolicus, has an 86-aminoacid cytochrome c -55 5s (AA c -55 5), in its ... identity between HT c -55 2 and AA c -55 5 (33%) is not as high as that between HT c -55 2 and PA c -55 1, but these three proteins still exhibit high sequence similarity (more than 50 %, Fig 1), therefore ... CYTOCHROMES c Fig 3D structural comparison of HT c -55 2 and PA c -55 1 The main-chain folding of HT c -55 2 (red) is overlaid with that of PA c -55 1 (green) To confirm experimentally the amino-acid...
... acquisition times of 55 and 53 ms in the 15N and 1H dimensions, respectively The total recording time was 16 h using 128 scans per FID The spectrum of SugE was taken at a Bruker DMX500 spectrometer ... wavelengths of 54 5 and 610 nm, respectively, with a band width of 2 .5 nm and a data pitch of 0.1 s Ten microliters of proteoliposomes (approximately 140 nM EmrE) in 15 mM Tris/HCl, pH 6 .5; mM dithiothreitol, ... (TPP; 50 lM), ethidium bromide (2 .5 lM) 16 and nigericine (5 lgÆmL)1) (Sigma) Green fluorescent protein (GFP) fluorescence was measured at excitation and emission wavelengths of 3 95 and 50 9 nm,...