VIBRATION AND SHOCK
... in./sec 0.001-10 in D 0.1-1 00Og Frequency Limits Tolerance 5- 500 Hz 5- 5 000 Hz 10 -5 000 Hz 5- 10 000 Hz 2- 25 OOP Hz ±2% ±2% ±1% ±2% 5% ... Small instrument motor armatures 0.000 05- 0.0001 0.0001-0.0002 0.0002-0.00 05 0.001-0.002 0.0001-0.0004 0.00 055 -0.0001 0.0001-0.0002 0.000 05- 0.0001 0.0000 05- 0.00001 vibrating with a large enough ... example: A - 0. 051 1/2Z) or A ~ 0. 05 II/ _ 10 ~ 051 1(100O)(IOOO) - 0.0001 957 in International System example A - 0.002 02/2Z) or D_ * 0.002 02/2 10 ~ 0.002 02(100O)(IOOO) - 0.004 95 mm This is about...
Ngày tải lên: 25/10/2013, 13:15
... H2O(l) HCl(g) HI(g) I2(g) NH3(g) NO2(g) O2(g) SO2(g) 51 .00 2 45. 3 5. 69 213.6 39. 75 42 .59 89.96 109.6 69.91 186.69 206.3 260 .57 192 .5 240. 45 2 05. 0 248 .5 Relative Standard Entropies States • the gas state ... H2O(g) HF(g) HBr(g) I2(s) N2(g) NO(g) Na(s) S(s) 28.3 152 .3 2.43 197.9 41.4 33.30 27. 15 130 .58 188.83 173 .51 198.49 116.73 191 .50 210.62 51 . 45 31.88 Al2O3(s) Br2(g) C(graphite) CO2(g) CaO(s) CuO(s) ... -110 .5 0 0 -241.82 -268.61 -36.23 0 +90.37 0 Al2O3 Br2(g) C(graphite) CO2(g) CaO(s) CuO(s) Fe2O3(s) H2O2(l) H2O(l) HCl(g) HI(g) I2(g) NH3(g) NO2(g) O2(g) SO2(g) -1669.8 +30.71 -393 .5 -6 35. 5 - 156 .1...
Ngày tải lên: 28/11/2013, 01:12
... 72. 25 c = 76. 65 b = 90.99 50 .0–1 .56 (1.62–1 .56 ) 1. 95 7.3 (39.6) 98.0 (92.4) 454 833 1060 85 4.4 (2.7) 18.1 (2.9) 19.8 BL44XU, SPring-8 0.9 P21 a = 69.06 b = 71.78 c = 76. 85 b = 91.81 50 .0–1. 35 (1.40–1. 35) ... 36.13–1 .57 (1.61–1 .57 ) 98731 ( 658 9) 19.8 (33.7) ⁄ 23.6 ( 35. 9) 0.008 1.1 35 6984 29 630 31.86–1. 35 (1.38–1. 35) 152 991 (10838) 18.8 (23.9) ⁄ 20.3 ( 25. 4) 0.008 1.170 6823 18 734 49. 75 1.48 (1 .52 –1.48) ... (1.40–1. 35) 1.94 4.8 (36.1) 98.0 (97.0) 117 951 2 164370 7.3 (6.9) 15. 9 (5. 8) 13.8 a = 69.06 b = 71.76 c = 76.40 b = 91.72 50 .0–1.48 (1 .53 –1.48) 1.92 7.7 (38.3) 95. 9 (88.9) 732 659 124631 6.1 (5. 2)...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... repressor J Mol Biol 223, 54 5 55 5 39 Lim, F & Peabody, D.S (1994) Mutations that increase the affinity of a translational repressor for RNA Nucleic Acids Res 22, 3748–3 752 40 Lim, F., Spingola, ... (p.f.u.ÆmL)1) Control MS2 T45S M88V D11N 3.4 kbar of pressure 4 .5 M urea 9.0 M urea 108 108 108 108 108 108 107 108 1 05 102 102 107 ND ND ND 102 and T45S particles with 4 .5 M urea resulted in drastic ... measurements, the sample was excited at 320 nm and the emission measured from 3 15 to 3 25 nm Standard buffer: 50 mM Tris/ 150 mM NaCl (pH 7 .5) Fluorescence data points are the average and standard deviation...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc
... activity (%) Wild-type S224A R226A R234A R242A 6.07 2.89 10.4 47.1 ND 66 59 85 68 56 1.29 0 .51 3.8 4 .5 (37) ( 35) (48) ( 15) (48) recovered activity to Eqn (4) and are summarized in Table They ... inactivation rate was pH-dependent and decreased at pH values below 6 .5 No signicant loss of activity was observed at pH 5. 0 5. 5 over 1 .5 h When 50 mM potassium phosphate or potassium sulphate was present ... PL-phosphorylase 1. 050 /80 50 /51 20 50 /80 Native phosphorylase 1. 050 /80 Activator oxyanion (mM) vmax (Uặmg)1) Km (mM) Phosphite (10) Phosphite (10) Phosphate (0.13.0) 1.86 0.11 1. 95 0.12 8.6 0.2...
Ngày tải lên: 19/02/2014, 16:20
VITAMINS IN FOODS Analysis, Bioavailability, and Stability potx
... 50 5 50 6 51 0 51 1 52 6 52 7 52 8 54 0 54 1 54 6 54 9 56 2 56 7 Chapter 21 58 5 58 5 58 5 58 5 58 7 58 8 58 9 59 1 59 2 59 2 59 2 59 4 59 8 59 8 600 612 612 612 624 624 6 25 626 638 638 639 21.2.6 Biotin ... 447 448 452 453 453 454 455 456 457 457 457 457 463 4 65 466 469 469 469 472 474 4 75 4 75 486 489 489 489 499 50 0 50 0 20 .5. 4 Vitamin E 20 .5. 4.1 Detection ... 143 1 45 146 149 149 149 150 150 150 151 151 152 154 154 155 155 156 7 .5. 2.1 Thiaminases 7 .5. 2.2 Polyphenols 7 .5. 3 Effects...
Ngày tải lên: 08/03/2014, 00:20
Báo cáo khoa học: Local changes in the catalytic site of mammalian histidine decarboxylase can affect its global conformation and stability pptx
... histamine by human small cell lung carcinoma Am J Pathol 160, 156 1– 156 5 Nathan, C (2002) Points of control in inflammation Nature 420, 846– 852 Medina, M.A., Urdiales, J.L., Rodrı´ guez-Caso, C., Ramı´ ... Biol Chem 259 , 52 14 52 21 Ohmori, E., Fukui, T., Imanishi, N., Yatsunami, K & Ichikawa, A (1990) Purification and characterization of 1-histidine decarboxylase from mouse mastocytoma P-8 15 cells J ... protein structures J Appl Crystallogr 24, 946– 950 30 Merritt, E.A & Bacon, D.J (1997) Photorealistic molecular graphics Methods Enzymol 277, 50 5 52 4 31 DeLano, W.L (2002) The PyMOL Molecular Graphics...
Ngày tải lên: 08/03/2014, 08:20
Arsenic speciation in environmental and biological samples extraction and stability studies
... 50 .0 ± 3.0 28.0 ± 1.9 85. 0 ± 3.7 50 .0 ± 2.9 18.0 ± 2.0 80.0 ± 3 .5 46.0 ± 2.2 12.2 ± 2 .5 68.0 ± 2.9 50 .0 ± 3.0 20.0 ± 2.2 89.0 ± 3 .5 82.0 ± 3.0 17.0 ± 2.0 99.0 ± 3.0 Certified values of NIST 156 8a ... Unknown peak As species 13.7 48.7 35. 0 97.4 ± ± ± ± 1.0 2.4 2.0 3.3 19 .5 51.0 28.0 98 .5 ± ± ± ± 2.0 3.0 1.6 3.9 15. 0 48.1 35. 0 98.1 ± ± ± ± 1.6 2 .5 2.0 3.6 18.4 50 .2 28.4 97.0 ± ± ± ± 1.0 3.0 2.0 ... 2.3 42.0 ± 1 .5 17.1 ± 1.0 73.0 ± 2.9 – – – Fish (68.3 ± 1.9) 1st 2nd Extractions n = 53 .0 ± 2.0 25. 0 ± 1.7 90.0 ± 3.1 58 .0 ± 2.2 27.0 ± 2.0 92.0 ± 3.0 56 .0 ± 2.1 24.0 ± 1.9 85. 0 ± 2.8 58 .0 ± 2.8...
Ngày tải lên: 15/03/2014, 23:49
Báo cáo " Boundedness and Stability for a nonlinear difference equation with multiple delay " docx
Ngày tải lên: 22/03/2014, 11:20
BIS Papers No 62 Financial sector regulation for growth, equity and stability doc
... Regulation for Growth, Equity and Stability in the Post Crisis World” 15 16 November 2011, Mumbai Day – 15 November 2011 11. 45 12. 45 Inaugural session - Addresses by D Subbarao, Governor, RBI and Jaime ... cannot grow by leveraging But, we have now turned the whole finance theory on its head This is because all economic theory and finance theory start from the assumption of normality But we are not living ... Implications for Growth and Financial Sector Regulation Anand Sinha 45 Summary of the discussion 85 Financial Sector Regulation for Equity Chair’s initial remarks Stephany Griffith-Jones...
Ngày tải lên: 23/03/2014, 02:20
Báo cáo khoa học: Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution doc
... 0.0 05 1.30 (1 .5) 0.013 Values between brackets indicate the ratio polymerization ⁄ hydrolysis 10 20 50 100 200 Wild-type nd nd 56 0 ( 45) 57 5 (57 ) 57 0 (52 ) 55 5 (42) H4 58 0 (62) 59 5 (84) 6 05 (108) ... (84) 6 05 (108) 600 (94) 6 05 (108) 58 5 (68) E9 nd nd 57 0 (52 ) 58 0 (62) 57 0 (52 ) 56 0 ( 45) FEBS Journal 273 (2006) 673–681 ª 2006 The Authors Journal compilation ª 2006 FEBS 6 75 Molecular evolution ... A1 159 G G1221T, G1839T A680G C123T, G1165C, A 150 9T C58T, G444T, T1793C N76D P 157 A, D231Y P234L, G 554 S E62K, D506N N387D Q613H E227G V389L, N503I R20C, F598S Improved Pol Improved Act ⁄ Pol Improved...
Ngày tải lên: 23/03/2014, 11:20
Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf
... bottom): 2, 5, 7, 10, 15, 20, 30, 45, 60, 75, 120, 150 and 180 lM The symbols in (B) correspond to various concentrations between and 180 lM at four different temperatures: 10, 15, 20 and 25 °C (h, ... conclusions Thus, for example, the DDSU, 25 values at 25 °C corresponding to those of DDGU, 25 in Table are )6.0, )5. 5 and )2.0 JÆK)1Æmol)1 at pH 4.00, 3. 75 and 3 .50 , respectively, and, given the known ... monomer concentration of 120 lM DGU, 25 and DDGU, 25 stand for the changes in standard Gibbs energy at 25 °C (DGU, 25) and their excess (DDGU, 25) above the DGU, 25 of the E34D mutant The Tg values...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: Solution structure and stability of the full-length excisionase from bacteriophage HK022 pot
... 25 °C FN defines the protein native state population pH Tm (°C) DH (Tm) (kJÆmol)1) DCp(Tm) (kJÆmol)1ÆK)1) DH° (kJÆmol)1) DS° (kJÆmol)1ÆK)1) DG° (kJÆmol)1) FN° 5. 5 5. 5a 6 .5 6.5b 7.0 41.6 47.3 51 .4 ... determined at pH 4.7, 5. 5, 6 .5 and 7 .5 (each buffer contained 100 mM NaCl) (C) The partial molar heat capacities of HK022 Xis_wt at 100 and 400 mM NaCl (50 mM sodium acetate at pH 5. 5 was used as a ... (kJÆmol)1ÆK)1) DG° (kJÆmol)1) FN° 5. 5 5. 5a 6 .5 6.5b 7.0 41.6 47.3 51 .4 51 .9 54 .0 138 153 1 65 1 65 174 2.8 2.8 3.1 3.0 3.0 82 82 76 74 74 0.26 0. 25 0.22 0.21 0.21 6.9 8.2 9.8 9.9 11.0 0.91 0.96 0.98 0.98 0.99...
Ngày tải lên: 23/03/2014, 15:21
Báo cáo Y học: Domain organization, folding and stability of bacteriophage T4 fibritin, a segmented coiled-coil protein docx
... coiled-coil transition 486 281 258 182 120 108 75 58 )9280 )3610 )3170 )1660 – )1216 )630 )51 5 )7600 )3080 )2640 )1170 )687 ) 656 – – – )53 0 )53 0 )490 – )56 0 – )51 5 0.13 0.39 0.42 0.88 0.91 0.81 ... Venyaminov for providing the CONTIN program This work was supported in part by HHMI (grants 751 95 52 080, and 55 000324), Russian Foundation for Basic Research (grant 99-04-48430), and by the ÔUniversities ... Biochemistry 40, 354 4– 355 2 30 Yu, M.-H & King, J (1984) Single amino acid substitutions influencing the folding pathway of the phage P22 tailspike endorhamnosidase Proc Natl Acad Sci USA 81, 658 4– 658 8 31...
Ngày tải lên: 24/03/2014, 03:21
Báo cáo Y học: Cytochrome c from a thermophilic bacterium has provided insights into the mechanisms of protein maturation, folding, and stability potx
... stability of HT c -55 2 (see below) For clarity, the residue numbers used for HT c -55 2 are those of PA c -55 1 A hyperthermophile, Aquifex aeolicus, has an 86-aminoacid cytochrome c -55 5s (AA c -55 5), in its ... identity between HT c -55 2 and AA c -55 5 (33%) is not as high as that between HT c -55 2 and PA c -55 1, but these three proteins still exhibit high sequence similarity (more than 50 %, Fig 1), therefore ... CYTOCHROMES c Fig 3D structural comparison of HT c -55 2 and PA c -55 1 The main-chain folding of HT c -55 2 (red) is overlaid with that of PA c -55 1 (green) To confirm experimentally the amino-acid...
Ngày tải lên: 24/03/2014, 03:21
Islamic vs. Conventional Banking: Business Model, Efficiency and Stability ppt
... 3114 6041 59 61 56 95 553 1 4966 4837 3332 6023 6036 61 65 6 059 33.790 2.460 62.412 2 .54 9 54 .58 3 5. 772 1. 351 8.098 17.718 1.290 11 .55 0 41 .53 5 19.808 6.398 28 .52 0 1.804 19.083 4 .53 4 1.4 45 7. 050 13.326 ... 0.109 16.866 85. 490 5. 411 13 .57 2 41.737 2.288 13.882 47 .51 4 1 .52 8 0.000 0.000 0.000 3 15 303 309 322 258 358 8.0 35 4.871 0. 657 47.360 0 .53 7 14.390 1 .52 8 3. 252 0.190 21.111 0.424 25. 892 5. 544 -13.127 ... -0.320 3. 650 7. 350 69.326 26.742 109.422 8.282 92.490 13.440 4.747 20.074 45. 676 3.340 25. 480 87.110 36. 755 3.241 61.287 3.221 55 .822 4.918 1.284 5. 944 16.700 1 .51 6 15. 355 40.794 33 .51 5 2.362 71.440...
Ngày tải lên: 29/03/2014, 08:20
Báo cáo khoa học: Metal ions modulate the folding and stability of the tumor suppressor protein S100A2 docx
... +Ca2+ (10 : 1) wt C2S 58 .4 66.6 56 .6 53 .9 56 .6 50 .6 DCys 59 .5 58.1 Aggregates 68.1 42 > 80 75 > 85 Apo S100A2-wt and S100A2-DCys have very similar T app values of 58 .4 and 59 .5 °C, respectively, ... 0.06 153 0 ΔAbs (a.u.) 0.04 0.02 0.00 –0.02 –0.04 153 0 cm–1 155 2 cm–1 1622 cm–1 1 651 cm–1 1.0 0.08 155 2 –0.06 0.8 0.6 0.4 0.2 0.0 1 651 –0.08 1700 1 650 1600 155 0 Wavenumber (cm–1) 20 30 D 50 60 ... mol)1) mol)1) Apo 4.7 +Zn2+ 2 .5 +Ca2+ 3.9 89.9 35. 1 40.3 13.0 3 .5 2.9 – )14.2 )5. 2 4 .5 4.0 4.8 78.9 50 .1 62.4 11.2 5. 3 7.0 – )3.9 +2.4 4.2 4.1 4.3 71.8 68.2 51 .8 10.3 9.7 5. 3 – )0.8 +0.8 a DDGU =...
Ngày tải lên: 30/03/2014, 02:20
Báo cáo khoa học: Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis ppt
... nm ⁄ E 455 nm ratio of 9.6 and an extinction coefficient, at 455 nm, of 12 800 m)1Æcm)1 was determined) In contrast, the W243I mutant was found to exhibit a remarkably higher E274nm ⁄ E 455 nm ratio ... 0.12 ± 0.01 86 ± 1 .5 ± 0.2 0.37 ± 0.03 95 ± 2d 1.6 ± 0.1d 1 35 940 230 900 340 4 65 370 7 15 0.04 ± 0.01 108 ± 1.4 ± 0.1 As described previously [9] b As described previously [ 15] c As described ... 47 .5 ANS fluorescence 50 .0 Urea Cm (M) Trp fluorescence 1.4 (1.8) FAD fluorescencec 1.8, 6.2 39.2 42.3 n.d 1.0 (2.0) 1.3, 5. 6 42.4 38 .5 41.7 n.d (1.9) n.d W243Y 40.6 41.2 40.2 0.8 (2.0) 1.1, 5. 0...
Ngày tải lên: 30/03/2014, 11:20
Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc
... acquisition times of 55 and 53 ms in the 15N and 1H dimensions, respectively The total recording time was 16 h using 128 scans per FID The spectrum of SugE was taken at a Bruker DMX500 spectrometer ... wavelengths of 54 5 and 610 nm, respectively, with a band width of 2 .5 nm and a data pitch of 0.1 s Ten microliters of proteoliposomes (approximately 140 nM EmrE) in 15 mM Tris/HCl, pH 6 .5; mM dithiothreitol, ... (TPP; 50 lM), ethidium bromide (2 .5 lM) 16 and nigericine (5 lgÆmL)1) (Sigma) Green fluorescent protein (GFP) fluorescence was measured at excitation and emission wavelengths of 3 95 and 50 9 nm,...
Ngày tải lên: 30/03/2014, 13:20