VIBRATION AND SHOCK
... 102 020 102 000 = 9-^A 10 nm 100 nm /mi 10 ptm 100 fjan mm 10 mm 1m fN (Hz) 980 576 49 8 157.6 49 .8 15.76 4. 98 0 .49 8 Note, from Eqs (23.2a) and (23.2b), that fN depends on 6, and thus on both M and ... r = in Then F - 0.10230/I6)(IXm)2 - 1 14 Ib If this centrifugal force of 1 14 Ib occurred in an electrical motor, for instance, whose weight was less than 1 14 Ib, the imbalance force acting through ... removing 2 .41 oz at a radius of in., or by removing oz at a radius of 2 .41 in or any convenient combination of weight and radius Let us now draw a vector diagram of forces at station 4, summing...
Ngày tải lên: 25/10/2013, 13:15
... H2O(g) HF(g) HBr(g) I2(s) N2(g) NO(g) Na(s) S(s) 28.3 152.3 2 .43 197.9 41 .4 33.30 27.15 130.58 188.83 173.51 198 .49 116.73 191.50 210.62 51 .45 31.88 Al2O3(s) Br2(g) C(graphite) CO2(g) CaO(s) CuO(s) ... HCl(g) HI(g) I2(g) NH3(g) NO2(g) O2(g) SO2(g) 51.00 245 .3 5.69 213.6 39.75 42 .59 89.96 109.6 69.91 186.69 206.3 260.57 192.5 240 .45 205.0 248 .5 Relative Standard Entropies States • the gas state ... C3H8(g) + O2(g) → CO2(g) + H2O(g) has ∆Hrxn = -2 044 kJ at 25°C Calculate the entropy change of the surroundings Given: Find: Concept Plan: ∆Hsystem = -2 044 kJ, T = 298 K ∆Ssurroundings, J/K T, ∆H Relationships:...
Ngày tải lên: 28/11/2013, 01:12
... (39.6) 98.0 (92 .4) 45 4833 106085 4. 4 (2.7) 18.1 (2.9) 19.8 BL44XU, SPring-8 0.9 P21 a = 69.06 b = 71.78 c = 76.85 b = 91.81 50.0–1.35 (1 .40 –1.35) 1. 94 4.8 (36.1) 98.0 (97.0) 1179512 1 643 70 7.3 (6.9) ... 18.8 (23.9) ⁄ 20.3 (25 .4) 0.008 1.170 6823 18 7 34 49.75–1 .48 (1.52–1 .48 ) 113539 (7965) 22.6 (29.5) ⁄ 25.6 (32.7) 0.012 1.382 6855 10 41 7 24. 5 39.1 14. 8 27.6 10.1 21.0 28 .4 16.0 91.8 8.0 92.9 7.1 ... = 76 .40 b = 91.72 50.0–1 .48 (1.53–1 .48 ) 1.92 7.7 (38.3) 95.9 (88.9) 732659 1 246 31 6.1 (5.2) 16.6 (3.6) 18.3 36.13–1.57 (1.61–1.57) 98731 (6589) 19.8 (33.7) ⁄ 23.6 (35.9) 0.008 1.135 69 84 29 630...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... Proc Natl Acad Sci USA 95, 9 244 –9 249 11 Ling, C.M., Hung, P.P & Overby, L.R (1969) Specificity in selfassembly of bacteriophages Q beta and MS2 Biochemistry 8, 44 64 44 69 12 Witherell, G.W., Gott, ... cause proteins to become less stable [44 ,45 ] Using the program VMD [46 ] and a probe ˚ radius of 1 .4 A, we analyzed the pdb coordinates of WT bacteriophage [ 14] and the M88V mutant (by substitution ... Chem 269, 9006–9010 41 Peabody, D.S & Chakerian, A (1999) Asymmetric contributions to RNA binding by the Thr45 residues of the MS2 coat protein dimer J Biol Chem 2 74, 2 540 3–2 541 0 42 Dertinger, D.,...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc
... enzyme activity (%) Wild-type S224A R226A R234A R 242 A 6.07 2.89 10 .4 47.1 ND 66 59 85 68 56 1.29 0.51 3.8 4. 5 (37) (35) (48 ) (15) (48 ) recovered activity to Eqn (4) and are summarized in Table ... reconstitution with 40 lM PLP and 50 mM potassium phosphate as described under Materials and methods Protein No oxyanion mM Sulphate mM Phosphate Wild-type S224A R226A R234A S238A R 242 A 57 40 11 260 36 ... effect of the mutation may be stabilizing (R234A, R 242 A), neutral (S238A, S224A), or destabilizing (R226A), compared to the wild-type Except for R226A and R 242 A mutants (Table 1), all enzymes were...
Ngày tải lên: 19/02/2014, 16:20
VITAMINS IN FOODS Analysis, Bioavailability, and Stability potx
... 20.5.3 .4 Normal-Phase Separations 20.5.3.5 Reversed-Phase Separations 43 9 43 9 44 0 44 0 44 0 44 1 44 2 44 2 44 4 44 4 44 4 44 7 44 8 45 2 45 3 45 3 45 4 45 5 45 6 45 7 45 7 45 7 45 7 46 3 ... Determinations 41 9 41 9 41 9 42 1 42 2 42 2 42 3 42 4 42 4 42 5 42 6 42 7 42 7 42 8 42 8 42 9 43 0 43 0 43 0 43 1 43 5 43 6 43 6 43 6 43 6 43 7 43 7 43 7 43 8 20 .4 HPLC Systems 20 .4. 1 Principle ... 25 26 27 28 30 32 32 33 33 34 34 35 36 Chapter 39 40 40 40 41 43 43 45 45 45 45 45 46 47 48 48 51 56 3.3.2 .4 Provitamin A Carotenoids 3.3.3...
Ngày tải lên: 08/03/2014, 00:20
Báo cáo khoa học: Local changes in the catalytic site of mammalian histidine decarboxylase can affect its global conformation and stability pptx
... Peptides 81, 41 48 Fleming, J.W & Wang, T.C (2000) Amino and carboxyterminal PEST domain mediate gastrin stabilization of rat 1-histidine decarboxylase isoforms Mol Cell Biol 20, 49 32 49 47 ´ Olmo, ... group and enhances catalysis J Biol Chem 278, 948 1– 948 8 48 Hayashi, H (1995) Pyridoxal enzymes: mechanistic diversity and uniformity J Biochem 118, 46 3 47 3 ... having typical absorption maxima around 43 0 nm [44 ] On the other hand, when using a-FMH, accumulation of a PLP-derivative with an absorption maximum at 345 nm can be clearly observed after the...
Ngày tải lên: 08/03/2014, 08:20
Arsenic speciation in environmental and biological samples extraction and stability studies
... species 15 .4 48.7 33.8 97.9 ± ± ± ± 2.0 3.0 2.8 4. 5 16.5 49 .5 33.0 99.0 ± ± ± ± 2.1 3.0 2.0 4. 2 14. 6 49 .4 34. 0 98.0 ± ± ± ± 2.0 3.0 2.5 4. 4 15.3 48 .7 33 .4 97 .4 ± ± ± ± 2.3 3.0 2.0 4. 3 AsB DMA ... 1.6 4. 5, 1.6–5 .4, 1.7 4. 5 and 2.1–2 .4 ranges for As(III), As(V), MMA, DMA and AsB, respectively The maximum RSD achieved was about 4% It has been demonstrated by monitoring both 40 Ar 35 Cl and 40 ... Unknown peak As species 13.7 48 .7 35.0 97 .4 ± ± ± ± 1.0 2 .4 2.0 3.3 19.5 51.0 28.0 98.5 ± ± ± ± 2.0 3.0 1.6 3.9 15.0 48 .1 35.0 98.1 ± ± ± ± 1.6 2.5 2.0 3.6 18 .4 50.2 28 .4 97.0 ± ± ± ± 1.0 3.0 2.0...
Ngày tải lên: 15/03/2014, 23:49
Báo cáo " Boundedness and Stability for a nonlinear difference equation with multiple delay " docx
Ngày tải lên: 22/03/2014, 11:20
BIS Papers No 62 Financial sector regulation for growth, equity and stability doc
... November 2011, Mumbai Day – 15 November 2011 11 .45 –12 .45 Inaugural session - Addresses by D Subbarao, Governor, RBI and Jaime Caruana, General Manager, BIS 14. 00–16.00 Session I on “Financial Sector ... cannot grow by leveraging But, we have now turned the whole finance theory on its head This is because all economic theory and finance theory start from the assumption of normality But we are not living ... Growth Chair’s initial remarks Andrew Sheng 41 Implications for Growth and Financial Sector Regulation Anand Sinha 45 Summary of the discussion 85 Financial...
Ngày tải lên: 23/03/2014, 02:20
Báo cáo khoa học: Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution doc
... 48 62 0.52 0 .43 0.86 0.018 0.009 0.0 14 0 .43 (1.2)a 0.05 0.36 (2.0) 0. 04 0. 64 (1.2) 0.11 112 300 102 Km1 kcat1 (mM) (s)1) Total activity Wild-type 4. 0 H4 4. 4 E9 4. 2 Hydrolysis Wild-type 2.5 H4 ... G691T,C 144 1T C698T,C1239T,G1597A G184A, C1317T, G1516A G99A, G495A, A1159G G1221T, G1839T A680G C123T, G1165C, A1509T C58T, G 444 T, T1793C N76D P157A, D231Y P234L, G554S E62K, D506N N387D Q613H E227G ... formation (H4)] are indicated in bold kcat1 ⁄ Km1 Km2 kcat2 (s)1ÆmM)1) (mM) (s)1) kcat2 ⁄ Km2 (s)1ÆmM)1) 0. 74 0.60 1.19 0.19 0. 14 0.28 71 165 82 1 .4 1.7 2.2 0.020 0.010 0.026 0.35 0.18 0. 54 0. 14 0.23...
Ngày tải lên: 23/03/2014, 11:20
Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf
... DGU,25 of the E34D mutant The Tg values refer to the condition DGU(Tg) ¼ pH 4. 00 3.75 3.50 HUTmar variants Wild type E34D Wild type E34D Wild type E34D 77.5 73 .4 73.2 69.3 45 .2 43 .2 DHU,m (kJÆmol)1) ... DGU,25 (kJÆmol)1) Tg (°C) 183 175 1 74 166 106 100 28.5 26.7 26.6 25.0 16.0 15 .4 101.9 98.0 97.8 94. 2 74. 4 73.0 ± ± ± ± ± ± 2 2 3 ± ± ± ± ± ± 0.3 0.3 0.3 0.3 0 .4 0 .4 DDGU,25 (kJÆmol)1) ± ± ± ± ± ± ... contribution to stability Biochemistry 41 , 155 24 15535 40 Jaenicke, R & Bohm, G (2001) Thermostability of proteins from ¨ Thermotoga maritima Methods Enzymol 3 34, 43 8 46 9 41 Welfle, H., Misselwitz, R.,...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: Solution structure and stability of the full-length excisionase from bacteriophage HK022 pot
... attenuation: application to Ó FEBS 2003 48 58 V V Rogov et al (Eur J Biochem 270) 44 45 46 47 48 49 50 51 three-dimensional HNCO experiments Angew Chem Int Ed Engl 32, 148 9– 149 1 Pristovsek, P., Lucke, C., ... Tyr2–Thr4), b2 (residues Ile30–Phe31), b3 (residues Val35–Lys36), b4 (residues Tyr41–His 44) and b5 (residues Val48–Lys49) – are colored in cyan The triproline segment (residues Pro32–Pro 34) is ... the ÔwingÕ b-sheet (residues 2 4, 35–36 and 41 44 of HK022 Xis and residues 16–18, 47 48 and 58–61 of the Mu repressor) could be superposed with an ˚ RMSD value of 0 .49 A, whereas superposition...
Ngày tải lên: 23/03/2014, 15:21
Báo cáo Y học: Domain organization, folding and stability of bacteriophage T4 fibritin, a segmented coiled-coil protein docx
... GCN4 leucine zipper Science 268, 43 6 43 9 24 Lumb, K.J & Kim, P.S (1995) A buried polar interaction imparts structural uniqueness in a designed heterodimeric coiled coil Biochemistry 34, 8 642 –8 648 ... Mesyanzhinov, V.V (19 94) Fibritin encoded by bacteriophage T4 gene wac has a parallel triple-stranded alpha-helical coiled-coil structure J Mol Biol 242 , 47 0 48 6 Cohen, C & Parry, D.A (19 94) Alpha-helical ... 75195–52080, and 550003 24) , Russian Foundation for Basic Research (grant 99- 04- 4 843 0), and by the ÔUniversities of RussiaÕ grant to V V M, and by Swiss National Science Foundation (grant 31 -49 281.96) to...
Ngày tải lên: 24/03/2014, 03:21
Báo cáo Y học: Cytochrome c from a thermophilic bacterium has provided insights into the mechanisms of protein maturation, folding, and stability potx
... interactions uniquely occur between Arg37 and Tyr 34 and/or Tyr43, the latter also having hydrophobic contacts with the side chains of Tyr 34, Ala40, and Leu 44 in HT c-552 These interactions are not found ... biochemical, spectral, and 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 structural characterization of the recombinant protein Protein Sci 9, 20 74 20 84 Arslan, E., Schulz, H., Zufferey, R., Kunzler, ... to Ile (V78I)] and double mutations [Phe7 to Ala/Val13 to Met (F7A/V13M) and Phe 34 to Tyr/Glu43 to Tyr (F34Y/ E43Y)] in the three regions of PA c-551 each individually enhanced the overall protein...
Ngày tải lên: 24/03/2014, 03:21
Islamic vs. Conventional Banking: Business Model, Efficiency and Stability ppt
... 17 64 1859 1587 2086 13 64 13 14 1502 1272 1372 8.078 4. 625 0.685 53. 744 0.505 2 .47 0 17.006 34. 738 7 .40 3 4. 0 74 10.982 1. 643 4. 521 0.196 19.712 0 .45 9 12.010 22 .41 3 30.3 54 2.051 1.561 2. 347 4. 442 ... 92 .49 0 2.650 54. 090 3 .49 5 62. 140 0.000 0.000 Total gross loans Total gross loans Total gross loans 21978 25383 147 47 4. 485 1. 344 6.2 04 4.050 1.510 6.3 64 0.660 -0.008 0.379 13 .44 0 4. 747 20.0 74 4.765 ... 21 24 2 148 2123 2107 2070 2098 1336 1767 1716 2 148 2016 Mean Standard deviation 1 .41 7 1. 143 12 .49 9 7. 044 18.737 13 .43 1 1.773 5 .42 1 64. 005 28.1 04 40.8 34 25.1 74 5.903 6.103 4. 705 4. 139 1.217 1 .41 2...
Ngày tải lên: 29/03/2014, 08:20
Báo cáo khoa học: Metal ions modulate the folding and stability of the tumor suppressor protein S100A2 docx
... mol)1ÆM)1) (kJÆmol)1) mol)1) mol)1) Apo 4. 7 +Zn2+ 2.5 +Ca2+ 3.9 89.9 35.1 40 .3 13.0 3.5 2.9 – ) 14. 2 )5.2 4. 5 4. 0 4. 8 78.9 50.1 62 .4 11.2 5.3 7.0 – )3.9 +2 .4 4.2 4. 1 4. 3 71.8 68.2 51.8 10.3 9.7 5.3 – ... crystal structure [8] and other C 10 –1 –1 –2 –2 –2 –3 4 –3 4 190 200 210 220 230 240 250 260 190 200 210 220 230 240 250 260 190 200 210 220 230 240 250 260 Wavelength (nm) Wavelength (nm) Wavelength ... Unfolded fraction 0.8 Unfolded fraction 40 0.6 0 .4 0.2 0.6 0 .4 0.2 Ca2+ Stabilization 0.0 Ca2+ Stabilization 0.0 30 40 70 50 60 Temperature (°C) 80 90 30 40 50 60 70 Temperature (°C) 80 90 Fig...
Ngày tải lên: 30/03/2014, 02:20
Báo cáo khoa học: Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis ppt
... activity assay mixture 506 d (3 34) ( 342 ) (338) ( 342 ) (338) ( 340 ) (337) ( 341 ) 18.9 9 .4 12.3 These values were determined in the presence FEBS Journal 273 (2006) 5 04 512 ª 2006 The Authors Journal ... DLOOPa,b W 243 I Temperature (°C) Trp fluorescence 46 .8 FAD fluorescence 47 .5 ANS fluorescence 50.0 Urea Cm (M) Trp fluorescence 1 .4 (1.8) FAD fluorescencec 1.8, 6.2 39.2 42 .3 n.d 1.0 (2.0) 1.3, 5.6 42 .4 38.5 ... values of 0. 84, 0.78, and 0.21 arbitrary unitsÆlm)1 for wild-type, W 243 Y and W 243 I DAAOs were obtained, whereas the Kd for ANS was practically unchanged ( 140 ± 20 lm for wild-type and Trp 243 mutants)...
Ngày tải lên: 30/03/2014, 11:20
Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc
... resulted in the refolding of the pumps Antimicrob Agents Chemother 41 , 44 0 44 4 proteins, and renaturation procedures with strong denatu 14 Aleshin, V.V., Zakataeva, N.P & Livshits, V.A (1999) A new ... transport protein EmrE FEBS Lett 48 0, 127–131 43 Yokoyama, S (2003) Protein expression systems for structural genomics and proteomics Curr Opin Chem Biol 7, 39 43 44 Betton, J.M (2003) Rapid translation ... Biol Chem 271, valuable tool for the rapid generation of samples suitable for 26 31 044 –31 048 structural analysis [43 ], and commercially available systems 11 Yerushalmi, H., Lebendiker, M & Schuldiner,...
Ngày tải lên: 30/03/2014, 13:20