autophagy during ageing in drosophila

... Publishing Ltd / University of Adelaide and Flinders University 2006 2006 ACCOUNTING FOR POPULATION AGEING 25 undertake a qualifying study and again this information is not available in the ... people in institutions or people living in remote areas, whereas these groups are included in the Census An alternative reweighting could be based on total numbers from the Census excluding these ... proportion in the working-age category, resulting in a larger proportion of the population on non-zero wage and salary income b) Population ageing, taxes and expenditure This section examines the...

Ngày tải lên: 17/02/2014, 10:20

... p48 proteins Immunostaining of meiotic C cinereus nuclei Immunostaining of meiotic C cinereus nuclei was perfomed as described in previous reports [29–31] with minor modifications C cinereus gills ... leupeptin, lgÆmL)1 pepstatin A, and Protease Inhibitor Cocktail (Roche)] Aliquots of 100 lL containing mg of crude extract protein were layered onto 1900 lL of a linear 15–35% glycerol gradient in ... amount needs to catalyze the incorporation of pmol of [3H]-d TTP into a DNA polymer in 30 Protein concentrations were determined using the Coomassie Brilliant Blue binding technique ND, not detected...

Ngày tải lên: 20/02/2014, 11:20

... Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation FASEB J 9, 313–323 Pinna, L.A & Meggio, F (1997) Protein kinase CK2 (Ôcasein kinase-2Õ) and its implication in cell ... Biosynthesis of casein kinase II ¨ in lymphoid cell lines Eur J Biochem 220, 521–526 12 Guerra, B., Siemer, S., Boldyreff, B., Issinger, O.-G (1999) Protein kinase CK2: evidence for a protein kinase CK2b ... protein in Drosophila tissues Western analysis using polyclonal anti-CK2btes Ig: R, recombinant CK2btes protein purified from E coli; T, protein extract from pairs of adult testes; C, protein extract...

Ngày tải lên: 21/02/2014, 15:20

... three proteins; dots indicate amino acids identical between two proteins The small box indicates the position of substituted amino acids within the mEH catalytic triad (Glu in DmEH; Asp in Jheh1 ... hormone metabolizing enzymes in two species of Drosophila during development Insect Biochem Mol Biol 26, 829–835 Engstrom, Y (1999) Induction and regulation of antimicrobial ¨ peptides in Drosophila ... precisely determined in this study Similar rapid and transient activations of self-defence protein genes in insects occur when insects are infected with microorganisms [35], in which case antimicrobial...

Ngày tải lên: 07/03/2014, 21:20

... replacement of a lysine residue in position 35 confirms the findings of Cavins and Friedman [12] and Bordini et al [16], who reported an interaction of acrylamide with the e-amino function of lysine residues ... activity after incubation in acrylamide ⁄ TEMED by the activity after incubation in buffer For determination of half-life, the activity of crude extracts was determined after incubation in the desired ... for serine Considering that Chiari et al [13] observed an at least twofold stronger effect of acrylamide on cysteine than on other amino acids, this is quite intelligible It is an interesting result,...

Ngày tải lên: 16/03/2014, 13:20

... an active ageing policy by investing in retaining older workers in the labour force, reinforcing their position in the labour market and reintegrating those currently left out by enhancing their ... persons in society and fostering social inclusion and independent living; promoting equitable and sustainable economic growth in addressing the implications of population ageing; strengthening and ... continuing declines in mortality at older ages in many of the countries – indicate that future ageing in this region will develop predominantly at the top of the age pyramid 30 Demography of ageing: ...

Ngày tải lên: 22/03/2014, 21:20

... generating a strand break containing a 5¢-sugar phosphate This is further processed by Pol b and XRCC1– DNA ligase IIIa heterodimer Cross-linking during incubation of the uracil-containing substrate ... poly(ADP-ribosyl)ation during BER of a uracil-containing oligonucleotide Inhibition of PARP-1 poly(ADP-ribosyl)ation prevents BER in cell extracts To examine whether PARP-1 is involved in every single BER ... excessive SSBs arising during BER or as a result of direct DNA damage Results Cross-linking of BER proteins during repair of damaged DNA Although it has previously been shown that PARP-1 binds to nicked...

Ngày tải lên: 23/03/2014, 13:20

... Gal(b1-3)GalNAc-binding lectin peanut agglutinin failed to reveal any signal in embryonic salivary glands [8], which could mean that salivary O-glycan chains are elongated, thus abrogating peanut agglutinin ... abrogating peanut agglutinin binding Furthermore, the peanut agglutinin staining in the developing nervous system documented by D’Amico and Jacobs [8] could not be confirmed in our in situ hybridization ... comprehensive testing of all core-1 b3GalT homologous genes during Drosophila development will show whether other genes are expressed in the tissues that are positive for peanut agglutinin binding Transcripts...

Ngày tải lên: 23/03/2014, 15:20

... be involved in O2 supply and, possibly, the hypoxia tolerance of Drosophila However, the globin might also be instrumental in alleviating oxidative stress by detoxifying harmful ROS molecules In ... during certain developmental stages The observation that Drosophila dmeglob1 protein exhibits ligand-binding properties and expression patterns that resemble those of other known insect globins ... Discussion Hypoxia-tolerance in insects Drosophila and other insects have been shown to be surprisingly hypoxia resistant [4,6,7,28] Genetic Hemoglobin expression in Drosophila screens [6,29],...

Ngày tải lên: 30/03/2014, 02:20

... unpredictably during standing on the platform in one of four directions as part of a balance training program Rogers et al [33] showed that either voluntary or waist-pull-induced step training reduced ... step initiation time The above-mentioned studies [30,32,33] and perturbation systems previously used in research, train compensatory responses during up-right standing and not during walking, ... intensive balance training that included equilibrium control exercises of firm and foam surfaces and/or weight training followed by months of low intensity Tai Chi training Oddsson et al [31]...

Ngày tải lên: 19/06/2014, 08:20

... backward counting during gait, comparing the performances during single and DT A second aim was to find out differences if any with an healthy control group Since gait and balance impairments increase ... 0.034) Discussion This study investigated cognitive and motor interference during gait in vestibular patients complaining of Page of persistent dizziness and unsteadiness after unilateral vestibular ... elderly subjects in order to rule out the effects of aging as previously suggested [20] The main finding was that patients demonstrated a significant worsening of backward counting during gait, compared...

Ngày tải lên: 19/06/2014, 08:20

... variability), from a single subject and the same clinician during the same day over the mean of three repetitions (inter-trial variability), from a single subject and the same clinician during two different ... analysis includes: inter-day variation, inter-trial variation, inter-tester variation Mean and SD are obtained from a single subject among five examiners over the mean of three repetitions (inter-session ... same clinician Test 3: all the markers were placed on the same subject during the same session by five different clinicians appropriately trained in the same way by the same clinician (inter-session...

Ngày tải lên: 19/06/2014, 08:20

... This resulted in upward and outward reaching movements using both shoulder and elbow rotations, resembling for instance reaching for a cup in a drawer Two sets of multi-joint reaching movements ... ranged from 15 to 60 points in initial research [4], and were even lower in later work with FM scores ranging from 15 to 40 points [13] Differences concerning the arm position during dynamic evaluations ... coupling Remarkable in this context is that reductions in elbow extension with increasing shoulder abduction torques have been observed even during slow arm movements [7] Then again, this study involved...

Ngày tải lên: 19/06/2014, 08:20

... assistance at the ankle joint during locomotor training may be important Few studies have examined the effect of mechanical assistance during locomotor training on lower limb kinematics and muscle ... chronic incomplete spinal cord injury gained functional walking ability after treadmill training with partial bodyweight support and therapist manual assistance [15] Locomotor training is a promising ... to wearing the powered orthoses during locomotor training A typical practice session allowed 10–15 minutes of stepping with the orthoses in each condition (total 30– 45 minutes of stepping) Breaks...

Ngày tải lên: 19/06/2014, 10:20

... 33.7 mL.kg-1.min-1 in summer and 39.1 mL.kg-1.min-1 in winter The subjects were assumed to be heat acclimatised during the summer experiments, and heat unacclimatised during the winter trials ... were removed and placed in individual sealed plastic bags The subjects were then instructed to shower without wetting their hair, abstain from drinking, eating, or urinating, and to ensure they ... Journal of Occupational Medicine and Toxicology 2008, 3:4 intestinal wall Glucose is added to the drinks in order to maintain blood glucose levels (avoid fatigue) during the work period Sweat is...

Ngày tải lên: 20/06/2014, 00:20

... polymerization by primase during DNA replication [1], snRNA hybridization to pre-mRNA sequences during intron splicing [2], and siRNA hybridization to mRNAs during gene silencing [3] In translation, the ... ribosomes select initiator re¼ gions in mRNA: base pair formation between the terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli,” Proceedings of the National ... candidate in the Department of Electrical and Computer Engineering at North Carolina State University (NCSU), Raleigh, NC He obtained the M.S degree in electrical engineering from NCSU, in 2003,...

Ngày tải lên: 22/06/2014, 22:20

... for insulin [6] Interestingly, Imp-L2 has been shown to bind human insulin, IGF-I, IGF-II and proinsulin, and its homolog in the moth Spodoptera frugiperda, Sf-IBP, can inhibit insulin signaling ... with insulin for binding to the insulin receptor (InR) and inhibit the autophosphorylation of InR [6] Furthermore, IGFBP-7 is suspected to be a tumor suppressor in a variety of human organs, including ... to high insulin levels and is sufficient to rescue the lethality resulting from dilp2-induced hyperinsulinemia It has previously been shown that Imp-L2 can bind human insulin and insulin-related...

Ngày tải lên: 06/08/2014, 18:21

... a point mutation leading to the arginine to histidine substitution R525H disrupted BunA-binding (the point mutation derived from the Madm allele 4S3; Figure 3e) Thus, Madm is a Bun-interacting ... with UAS-bunA using a wing driver (C10-Gal4) caused an overgrowth phenotype in the wing (Figure 5i,j) We observed additional tissue between the wing veins, resulting in crinkled wings Thus, Madm ... regulators as well as in a proteomic screen for BunA-interacting proteins, and we show that BunA and Madm cooperate in promoting growth during development Results Long human TSC22DF proteins can substitute...

Ngày tải lên: 06/08/2014, 19:21

... (morning and evening), beginning days before starting radiotherapy and continuing everyday during radiotherapy In the other control group, an the identical-appearing placebo was administered in ... treatment of gynecological cancer Maintaining intestinal integrity during radiotherapy significantly influences the quality of life of patients Usually severe radiation-induced diarrhea is treated by ... changes in intestinal flora mediated through fermented milk intake FEMS Immunol Med Microbiol 1994, 10:55-64 19 Salminen E, Elomaa I, Minkkinen J, Vapaatalo H, Salminen S: Preservation of intestinal...

Ngày tải lên: 09/08/2014, 08:23

... Aggregates were incubated with 50 μL of the double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, ... minutes, resuspended in complete DMEM (containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin), and plated at to × 107 nucleated cells per 75 cm2 flask (Falcon, Beckton Dickinson ... suspended to a concentration of × 106 cell/mL in serum-free DMEM containing mM pyruvate, 1% ITS + Premix (insulin, transferrin and selenous acid containing culture supplement), 37.5 mg/mL ascorbate2-phosphate...

Ngày tải lên: 09/08/2014, 14:22