augmentation of cell growth overexpression of protein tyrosine kinases

... overexpression of Tec in the T -cell line Jurkat synergizes with the action of phorbol myristate acetate to induce activation of the nuclear factor of activated T cells, whereas Itk overexpression has no ... the survival of developing B lymphocytes and the maturation of spleen B cells [41–43] Abnormal activation of NF-jB has been shown to be a hallmark of the development and progression of lymphoid ... lower than that of Itk and Btk in T cells and B cells, respectively In B cells, Tec expression is approximately 5-fold higher than in T cells, but Tec is upregulated in activated T cells and in...

Ngày tải lên: 07/03/2014, 00:20

... substrates of rPTPa Expression of rPTPa in cells can inhibit some of insulin mediated effects For example, expression of rPTPa in BHK-IR cells inhibits insulinmediated cell rounding and growth inhibition ... Activation of the IR results in the formation of a protein complex involving the scaffolding proteins CAP and Cbl Subsequent tyrosine phosphorylation of Cbl by the IR leads to translocation of the ... domain of SHP-2 and phosphotyrosine 1146 of the activated insulin receptor [66] Insulin also induces the formation of a complex of IRS-1 and SHP-2, requiring the tyrosines 1172 and 1222 of IRS-1...

Ngày tải lên: 17/03/2014, 23:20

... regulation of T -cell activation through kinases and phosphatases Biochem J 2003, 371:15-27 Qu CK, Nguyen S, Chen J, Feng GS: Requirement of Shp-2 tyrosine phosphatase in lymphoid and hematopoietic cell ... localization of intracellular protein tyrosine phosphatases in T cells Eur J Immunol 2000, 30:2412-2421 15 Sozio MS, Mathis MA, Young JA, Walchli S, Pitcher LA, Wrage PC, et al.: PTPH1 is a predominant protein- tyrosine ... detected the withdrawal of the paw Latency of each paw withdrawal was recorded and mean values of left and right paws were used as reaction index for the individual animal A cut-off of 25 seconds was...

Ngày tải lên: 11/08/2014, 03:20

... Function of the PRL Family of Protein Tyrosine Phosphatase Major Professor: Stephen K Randall The PRL family of enzymes constitutes a unique class of protein tyrosine phosphatase, consisting of three ... evaluation of the two sections The percentage of positive cells was estimated as the number of stained cells, per total number of cells counted The localization of staining within the cells of each ... evaluation of the two sections Percentage of positive cells was estimated as the number of stained cells per total xxiv 1404 number of cells counted To confirm reproducibility of the analysis, 25% of...

Ngày tải lên: 24/08/2014, 11:44

... homolog deleted on chromosome 10 Protein tyrosine phosphatase Protein tyrosine kinase Protein tyrosine phosphatase alpha Polyvinylidene difluoride Proline-rich tyrosine kinase Radioimmunoprecipitation ... DKO cells exhibit defective integrin-induced cell adhesion …………… 93 4.4 α/s DKO cells exhibit an integrin-mediated cell spreading defect that is more severe than that of PTPα-/- and Src-/- cells ... 1.2 Protein tyrosine phosphatase (PTP) superfamily 1.3 Catalytic mechanism of protein tyrosine phosphatases………… 1.4 Receptor-like PTPs and their features ……………………………… 1.5 Receptor-like tyrosine...

Ngày tải lên: 14/09/2015, 12:02

... corresponding residue interacts with a phosphotyrosine in the AL of that protein [21] This Arg745 flip is not simply a consequence of phosphorylation of the AL tyrosine pTry799; in the substrate-bound ... the selectivity of this peptide for EphA3 over other ephrin receptor kinases gives insight into substrate specificity for this biologically relevant class of receptor tyrosine kinases and provides ... is a roughly equimolar mixture of the 18 amino acids excluding cysteine, and tyrosine In each peptide, one of the X positions was replaced with of 22 residues (one of the 20 unmodified amino acids,...

Ngày tải lên: 16/03/2014, 02:20

... different cell lines, HeLa, human epithelial cells, and NIH ⁄ 3T3, murine fibroblasts Visualization of the fluorescent chimera proteins, ER, isoform ⁄ a of PDIP46 ⁄ SKAR or isoform ⁄ b of PDIP46 ... isoform ⁄ a of PDIP46 ⁄ SKAR The asterisk indicates the peptide identified by manual analysis of MS ⁄ MS spectra Comparison of the intracellular localizations of the human ER and PDIP46/SKAR proteins ... total number of cells per wing and a reduction in the area of individual cells [8] If indeed ER is involved in the cell growth control a mutation in ER could add to the effects of an r mutation...

Ngày tải lên: 19/02/2014, 05:20

... Phosphorylation of the T -cell receptor f chain (Eur J Biochem 270) 2373 Fig 1H-NMR spectroscopy of His-cTCRf (A) Expansion of the 1D 1H spectrum of His-cTCRf The aromatic resonances of the tyrosines ... the levels of each phosphotyrosine staying similar over the following 60 The average levels of phosphorylation of each ITAM tyrosine after 30 of phosphorylation are presented in Table Tyrosine ... restriction to Lck accessing certain tyrosine residues of the protein chain Our studies have determined the efficiency of phosphorylation of each of the six f ITAM tyrosines by PTK Lck, from which...

Ngày tải lên: 08/03/2014, 02:20

... expressed at growth arrest of mammalian cells Cell 1988, 54:787-793 Manfioletti G, Brancolini C, Avanzi G, Schneider C: The protein encoded by a growth arrest-specific gene (gas6) is a new member of the ... are pleiotropic inhibitors of the innate immune response Cell 2007, 131:1124-1136 Lu Q, Lemke G: Homeostatic regulation of the immune system by receptor tyrosine kinases of the Tyro family Science ... analysis of the data and wrote parts of the manuscript AL participated in the design of the clinical study, included and followed patients, assisted in analysis of the data and wrote parts of the...

Ngày tải lên: 13/08/2014, 21:21

... breast cancer cell Icaritin increased MCF-7 breast cancer cell proliferation at doses less than 10 µM However, the combination of µM of icaritin with 100 pM estradiol inhibited the cell growth induced ... Summary of Herba Epimedii extracts and flavonoid and its effects on cancer growth 34  Table 1.7 Summary of effect of icaritin on cancer growth 38  Table 1.8 Concentration-time profiles ... Physiological roles of estrogens Estrogens are pivotal hormones in the progression of the majority of human breast cancers Estrogens influence growth, differentiation and function of tissues of the female...

Ngày tải lên: 04/10/2015, 17:05

... characterization of a recombinant Yersinia protein tyrosine phosphatase J Biol Chem 267, 23759–23766 20 Zhang ZY (1995) Kinetic and mechanistic characterization of a mammalian protein- tyrosine phosphatase, ... stereoview of a least squares superposition of unoxidized (yellow) (Protein Data Bank: 2PSZ) and oxidized (gray) (Protein Data Bank: 2PT0) PhyAsr, with oxidized PTP1B (light blue) (Protein Data ... conformation (orange) (Protein Data Bank: 1PTV) (B) Divergent stereoview of a least squares superposition of unoxidized (yellow) (Protein Data Bank: 2PSZ) and oxidized (gray) (Protein Data Bank:...

Ngày tải lên: 18/02/2014, 18:20

... increasing concentrations of choline abolishes daughter cell separation at the end of cell division, causing the formation of long chains of cells with only small effects on cell growth and viability, ... active site of Pce Effect of choline analogs on cell growth and viability Fig Effect of choline and analogs on the activity of cell wall lytic enzymes Data are shown as percentage of activity ... catalyzes the cleavage of the N-acetylmuramoyl-l-alanine bond of the peptidoglycan backbone [8] It is involved in the separation of the daughter cells at the end of cell division and in cellular autolysis...

Ngày tải lên: 19/02/2014, 05:20

... Various extracellular proteinases regulate cellular functions by degrading or processing protein substrates including extracellular matrix proteins, growth factors and cell surface proteins For ... types of the cell lines used are described in ‘Experimental procedures’ Fig Effects of proteinase inhibitors on processing of IGFBP-rP1 in culture of OVISE cells OVISE ovarian carcinoma cells ... Assay of IGFBP-rP1-cleaving activity after SDS/PAGE separation of proteinases IGFBP-rP1-cleaving proteinases present in the membrane fraction of OVISE cells and conditioned medium of DLD-1 cells...

Ngày tải lên: 19/02/2014, 07:20

... indicating the dose-dependent effect of miR-9 on the inhibition of cell proliferation (Fig 2B) We also found that overexpression of miR-9 suppressed cell growth in a time-dependent manner Although ... ES-2 cells and cell growth activity was detected using a MTT assay It was indicated that knockdown of NF-jB1 suppressed cell growth in a time-dependent manner A significant reduction in cell proliferation ... (Fig 6B), similar to the effect of pri-miR-9 on ovarian cell growth (Fig 2C) These results are consistent with the finding that overexpression of miR-9 suppresses cell growth in vitro, providing further...

Ngày tải lên: 07/03/2014, 00:20

... degradation of IGFBP-1 isoforms The y-axis shows the percentage of the ratio between the spot area of the degraded and that of the untreated protein measured using IMAGE J 1.37V software the presence of ... propensity of the different isoforms of IGFBP-1 to backbone cleavage in the presence of this partially purified specific protease Results Purification of IGFBP-1 isoforms The amniotic fluid proteins ... process can be the consequence of a combination of biological variables, including different activity levels of both the intracellular protein kinases and the extracellular phosphatases, this discrepancy...

Ngày tải lên: 07/03/2014, 00:20

... membrane-distal domain of receptor protein- tyrosine phosphatase alpha Biochemistry 38, 914–922 Lim KL, Ng CH & Pallen CJ (1999) Catalytic activation of the membrane distal domain of protein tyrosine phosphatase ... between intracellular domains of receptor protein- tyrosine phosphatases J Biol Chem 277, 47263–47269 Wallace MJ, Fladd C, Batt J & Rotin D (1998) The second catalytic domain of protein tyrosine phosphatase ... (2009) Large-scale structural analysis of the classical human protein tyrosine phosphatome Cell 136, 352–363 10 Wang Y & Pallen CJ (1991) The receptor-like protein tyrosine phosphatase HPTP alpha has...

Ngày tải lên: 15/03/2014, 10:20

... subcellular relocation of protein phosphotyrosine phosphatase 1B (PTP-1B) in human platelets EMBO J 12, 48434856 20 Barford D, Flint AJ & Tonks NK (1994) Crystal structure of human protein tyrosine ... than in calpain knockout or rescued cells, possibly reecting a role for calpain in the regulation of basal activity of these pathways Phosphorylation of a protein of  120 kDa, which reacted with ... Nat Rev Mol Cell Biol 7, 833846 Flint AJ, Gebbink MF, Franza BR Jr, Hill DE & Tonks NK (1993) Multi-site phosphorylation of the protein tyrosine phosphatase, PTP1B: identication of cell cycle...

Ngày tải lên: 16/03/2014, 00:20

... (1999) Cells of Escherichia coli contain a protein- tyrosine kinase, Wzc, and a phosphotyrosine -protein phosphatase, Wzb J Bacteriol 181, 3472–3477 26 Bugert P & Geider K (1997) Characterization of ... phosphorylation of the tyrosine The observation of dimeric YwlE having a phenylalanine residue in the dimer interface in place of a tyrosine confirms that lmwPTP dimerization is independent of phosphorylation ... weight protein tyrosine phosphatases: small, but smart Cell Mol Life Sci 59, 941–949 Conserved weak protein interactions in lmwPTP Tonks NK (2005) Redox redux: revisiting PTPs and the control of cell...

Ngày tải lên: 16/03/2014, 02:20

... Assay of long-term cell growth and morphological examination To determine the long-term growth potential of the Lynexpressing transformants, cells were incubated at an initial density of ´ 105 cellsámL)1 ... the case of mouse myeloid cells MPO gene expression Neutrophilic MPO is expressed when GM-I62M cells are cultured in the presence of G-CSF [26] Expression of MPO is one of the markers of neutrophilic ... nuclear lobulation was affected in these cells Therefore, tyrosine phosphorylation of cellular proteins was examined by immunoblot analysis of total cell lysates prepared from GM-I62M, GM-Lyn,...

Ngày tải lên: 17/03/2014, 17:20

... cell growth arrest These findings fit the general observation of various cell types that serum withdrawal induces cell growth arrest and/or apoptosis [27,28] whereas cell adhesion promotes cell ... error of the mean *P < 0.05 Growth kinetics of monolayer cultures From a seeding population of 20 × 103 cells per well (in sixwell plates), control monolayer cultures expanded to 166 ± 37 × 103 cells ... deprived of nutrient factors for the following parameters: (a) the harvested cell number, (b) the proportion of Ki-67-immunopositive cells, (c) cell viability, and (d) the proportion of SA-βgal-positive...

Ngày tải lên: 09/08/2014, 10:23