atf creb family of transcription factors in breast cancer

... MyoD/E-box binding factors The introduction of mutation into one of the putative Etsbinding sequences suppressed the promoter activity In addition, the specific binding of a nuclear protein to the ... aberrant expression of a3b1 integrin in tumor cells and their malignant behavior The increased expression of a3b1 integrin in gastric carcinoma cells is associated with their increased invasion and ... presence of a CCAAT box seems to be a characteristic feature of the a3 integrin gene among integrin a subunit genes Fig Flow cytometric analysis of the expression of the integrin a3 subunit in gastric...

Ngày tải lên: 21/02/2014, 03:20

... mobility shift assay of the FocB protein binding to DNA in the absence or presence of the DNA-binding compounds distamycin (minor groove-binding) and methyl green (major groove-binding), respectively ... is a minor groove-binding protein In general, minor groove-binding proteins show various degrees of sequence specificity when binding to DNA The lack of unique chemical features present within the ... on a5 Interestingly, substitution of Arg61 and Arg81 impair DNA-binding in PapB, indicating that these residues are critical for DNA-binding also in FocB [25] The DNA-recognition domain of RNA...

Ngày tải lên: 15/03/2014, 23:20

... domain; by contrast, the PY motif is missing in AP-2␦ but the amino- and carboxy-terminal ends of the core sequence of the transactivation domain are still conserved In addition, the binding affinity ... various proteins (see Table for a comprehensive list) Interacting proteins either modulate the activity of AP-2 proteins or are influenced in their function by binding to AP-2 proteins The tissue ... Dimerization DNA binding Figure A schematic representation of the protein structure of an AP-2␣ dimer, showing the proline- and glutamine (P/Q)-rich transactivation domain (89 amino acids, red),...

Ngày tải lên: 14/08/2014, 16:20

... Protein-protein interactions control cellular organization and dynamics in diverse biological processes including transcription Studying transcriptional initiation and the interplay between the transcription ... linker histone hH1 and hH3 and hH4 in the context of the nucleosome The split-ubiquitin system is uniquely suited for screening of transcription factor-binding proteins It does not rely on transcriptional ... future Identification of linker histone-interacting proteins might yield interesting insights regarding various regulatory processes that control chromatin dynamics 15 CHAPTER ONE INTRODUCTION 16 1.1...

Ngày tải lên: 29/09/2015, 13:01

... measurements of each of the nine points of irradiated skin were compared with corresponding points of non-irradiated skin by using a t-test This test determined the significance of differences ... measurement points taken in patients with Grade Figure Erythema and dry desquamation seen in Grade grade 4) showed a convincing trend of an increased probability of thinning in the skin thickness ... variation in skin thickness in the conservatively managed breast This finding suggests a need to use the same examination point when performing serial and comparative examinations The results of this...

Ngày tải lên: 09/08/2014, 09:20

Ngày tải lên: 09/08/2014, 09:20

... fusions In breast cancer, recurrent gene fusions have only been identified in rare subtypes, such as ETV6-NTRK3 in secretory breast carcinoma [12] and MYB-NFIB in adenoid cystic carcinoma of the breast ... the inhibition of apoptosis [29,30] IKZF3 is located at the most common telomeric breakpoint of the ERBB2amplicon [31] Interestingly, our preliminary analysis of clinical breast cancers shows ... membrane protein-associated protein B and C) and the carboxyterminal part of IKZF3, which harbors two Zn-finger domains IKZF3 was only detected as a fusion transcript, indicating activation of a quiescent...

Ngày tải lên: 09/08/2014, 22:23

... verification Haematoxylin and eosin (H&E) staining and immunohistochemical staining are shown on pathology sections of tumors implanted in NOD/SCID mice In a, b and c, the staining showed a single cell type ... be one of the causes of high expression of the drug-metabolising enzyme CYP1B1 in cancerous tissues [14] Finally, as a tumor suppressor in breast cancer cells, miR-17-5p regulates breast cancer ... Ras-GAP binding proteins (G3BP) Oncogene 2007, 26(29):4209-4215 27 French J, Stirling R, Walsh M, Kennedy HD: The expression of Ras-GTPase activating protein SH3 domain-binding proteins, G3BPs, in...

Ngày tải lên: 10/08/2014, 10:20

... expression in breast carcinomas, its role in breast cancer initiation and progression remains unclear The study presented here seeks to characterize ANXA1 in initiation and tumorigenesis of breast cancer ... membrane binding, freeing the NH2-terminal domain for interaction with S100A11 (Weng et al., 1993) This thus highlights some interesting mechanistic involvement of the NH2-terminal domain of ANXA1 ... effect of ANXA1 on the proliferation of tumor-derived cell lines The status of ANXA1 in certain clinical cancer tissues has been tabulated in Table Table 1: The status of ANXA1 in clinical cancer...

Ngày tải lên: 10/09/2015, 09:04

... higher in normal breast cell line (MCF10A) than breast cancer cell lines (MCF7, SK-BR-3, and MDA-MB-231) Proliferation rate in breast cancer cell lines Up-regulation of p53 in breast cancer cell lines ... the finding of early markers for therapeutic intervention that has disease modifying effect, in hope of bringing down death due to breast cancer In addition, the contribution of PrP to breast cancer ... (WHO, 2008), while according to the Singapore Cancer Registry, in 17 women will develop breast cancer in her lifetime in Singapore The risk of getting breast cancer increases with age, with the...

Ngày tải lên: 12/10/2015, 17:36

... during chondrogenesis and the involvement of transcription factors (henatoxylin and eosin-stained section of an embryonic cartilaginous limb) of the development of neural crest derivates, including ... factor AP-2 involved in DNA binding J Mol Biol 301, 807–816 Wankhade S, Yu Y, Weinberg J, Tainsky MA & Kannan P (2000) Characterization of the activation domains of AP-2 family transcription factors ... suppressor, the loss of AP-2a is caused by a failure in post-transcriptional processing of the protein [41] Additionally, it is evident that AP-2 transcription factors can indirectly modulate...

Ngày tải lên: 16/02/2014, 09:20

... recognize specifically Ets motifs flanking the +1 site (A) The binding of nuclear factors is eliminated by mutations in Ets motifs The binding of nuclear factors present in SK-N-SH (K) or SH-SY5Y (H) ... extents in each cell type Hence it is likely that Ets elements play a major role in determining both the level of expression and the location of the start of transcription of PS1 In the Ets family of ... determine activity through protein–protein interactions between Ets factors and factors binding at adjacent sites, as well as cofactors that not bind DNA themselves [29,30] Hence the specific set of...

Ngày tải lên: 16/03/2014, 18:20

... sensitivity as indicated by significantly increase in inhibition of germination in the overexpression line relative to that of wild type (Figure 1C) Increased inhibition of germination in the WRKY60overexpressing ... transiently induced during exposure to low non-freezing temperature in ABA-independent manner and exhibits continuous induction during dehydration and freezing treatment [21] In tobacco, a WRKY transcription ... growing evidence that plant WRKY transcription factors are involved in plant ABA signaling and abiotic stress responses In rice and barley, ABA induces expression of a number of WRKY genes in...

Ngày tải lên: 11/08/2014, 11:21

... conserved cis-acting element, which contains two distinct protein binding sites: the prolamin-box (PB) and the GCN4-like motif (GLM) Four transcription factors (TFs) are the gibberellin-regulated ... (GAMYB), the barley leucine zippers and (BLZ1, BLZ2), and the barley prolamin box binding factor Page of 11 (BPBF) that were shown to be involved in the transcription of B-hordeins encoded by the ... subgroup indicating footprints of selection on BLZ1, BLZ2 and BPBF However, selection might act in different ways: In case of BLZ2 selection resulted in the accumulation of a large number of low...

Ngày tải lên: 12/08/2014, 03:21

... (blocked arrows) of different stages in the proposed pathway are indicated Genistein is an inhibitor of tyrosine protein kinase and competitive inhibitor of ATP in other protein kinase reactions; ... the significance of maintaining reducing conditions in cells and the fight against the damaging effect of ROS intermediates [17,22,35,37–39] Oxidants, for instance, can cause carcinogenesis, sclerosis, ... nucleus, pull NF-κB off DNA, and export NF-κB back to its resting state in the cytoplasm Thick lines indicate the activating pathway; thin lines indicate the inactivating pathway IκB-independent pathways,...

Ngày tải lên: 12/08/2014, 18:20

... leads to increasing intracellular stores of GSSG, a potent inhibitor of NF-κB transcription factor DNA binding The pathway leading to the formation of GSH by the action of γ-glutamylcysteine synthetase ... binding protein beta, Sp-1, AP-1 and CREB are present in the promoter regions of numerous cytokine genes, including those whose expression is increased after blood loss To investigate early transcriptional ... activation of both NFκB and CREB in lung cell populations, only CREB activation was reduced in the mice treated with lisofylline Furthermore, lisofylline diminished hyperoxia-associated increases in...

Ngày tải lên: 12/08/2014, 19:21

... however, to indicate HIF-1 as a possible regulator of the evolution and propagation of the inflammatory process The rate of transcription of several genes encoding proteins involved in oxygen and ... potential mediator of increased pulmonary vascular permeability in this model of acute lung injury Further elaborating on the mechanisms involving HIF-1 in regulating the inflammatory response, ... hemorrhagic shock (HS) initiates an inflammatory response that includes increased expression of inducible nitric oxide synthase and production of prostaglandins [53] Induction of inducible nitric oxide...

Ngày tải lên: 12/08/2014, 19:21

... x3) Brown color indicates CK19 positive staining Arrows indicate bile duct staining Arrowheads indicate hepatocytic staining PV, portal vein; BD, bile duct Scale bar = 100 μm injuries towards ... pointing to the source of the likely pool of hepatocytes capable of undergoing transdifferentiation These results are also consistent with our previous findings indicating that subpopulation of ... signaling occurs through HGF and/or EGF via PI3 kinase regulating expression of HNF transcription factors that in turn lead to transdifferentiation Overall, understanding of transdifferentiation of...

Ngày tải lên: 13/08/2014, 13:20

... promoters of GPD for that model [116] dIncluding 17 input genes with predicted p53 binding sites in cluster A p53 is shown in bold eIncluding 26 input genes with predicted NF-κB binding sites in cluster ... protein factors) , ECAT (enhancer of CCAAT binding factors) , or PCAT (promoter of CCAAT binding factors) p53 binding motifs were also displayed '(+)' and '(-)' refer to strand direction of transcription ... included 17 genes with p53 binding sites in cluster A and 26 genes with NF-κB binding sites in cluster B In cluster A genes with p53 binding sites, putative models containing three and four transcription...

Ngày tải lên: 14/08/2014, 07:21

... homeodomain TFs that adjoin the same progenitor domain boundary [50] In the developing brain, cross-repressive interactions between Otx2 and Gbx2 define the midbrain-hindbrain boundary [51] and interactions ... Multalin [http://prodes.toulouse.inra.fr/multalin/multalin.html] Zhang B, Schmoyer D, Kirov S, Snoddy J: GOTree Machine (GOTM): a web-based platform for interpreting sets of interesting genes using ... Idb2, not bind DNA and instead function by interacting with other TFs [5] Another possibility could be that the promoter regions of the genes or the DNAbinding preference of the TFs we obtained have...

Ngày tải lên: 14/08/2014, 08:20