assessing protein ultrafiltration membrane interactions using flow field flow fractionation

... levels of mature SP-C in the alveoli [7–11] proSP-C is a 197-residue transmembrane protein with a type II orientation in the ER membrane; that is, the N-terminus is localized on the cytosolic side ... are all made as transmembrane precursors that are processed into fragments by proteolysis Recently, the Brichos domain has been found also in other proteins, including a protein (TFIZ1) that ... nonreduced protein (data not shown) The melting curves of membrane- bound CTC showed two transitions with maxima at 59 °C and 66–67 °C (Fig 8B) The first, second and third heat capacity curves of membrane- bound...

Ngày tải lên: 07/03/2014, 05:20

... a rapid and reliable method for the detection of protein protein interactions using yeast G -protein signaling In our system, protein protein interactions were detected utilizing the knowledge ... study of protein protein interactions Curr Biol 8, 1121–1124 Ehrhard KN, Jacoby JJ, Fu XY, Jahn R & Dohlman HG (2000) Use of G -protein fusions to monitor integral membrane protein protein interactions ... of detection of protein protein interactions have been developed using G -protein signaling Medici et al established the Gpa1–Gas chimeric system in which a receptor is fused to protein X and Gpa1–Gas...

Ngày tải lên: 16/03/2014, 01:20

... membrane and methylated amine membranes Table 6-3 Membrane characterization data and protein separation fluxes of different types of membranes Table 6-4 Mechanical strength and stability of membrane ... flat membranes ………………………… 153 6.2.4 Membrane characterization …………………………………………………….154 6.2.5 HPLC analyses of protein solution …………………………………………… 157 6.2.6 Protein separation by anion exchange membrane ... isoelectric focusing This study firstly investigated the feasibility of a combination of membrane technology and the Free -Flow Isoelectric Focusing (FFIEF) technology for a high-performance protein...

Ngày tải lên: 14/09/2015, 08:39

... very fast for the investigation of specific biomolecular interactions including protein- antibody interaction, protein- DNA interaction, protein- cell interaction, DNA-DNA interaction and etc [10,11] ... immobilized G-15 and S-15 DNA film in buffer using a Voigt-based viscoelastic model 78 Table 5-4 Analysis of the aptamer (G-15) and thrombin-aptamer complex in buffer using a Voigt-based viscoelastic model ... deoxyribonucleic acid DPV: differential pulse voltammetry ds: double-stranded E: electric field E0: electric field amplitude Edissipated: energy lost (dissipated) Estored: total energy stored EIS:...

Ngày tải lên: 03/10/2015, 20:32

... VBM-containing proteins to VCP As discussed above, several proteins bind the N-domain of VCP in a mutually exclusive manner, including the VBM-containing proteins Atx-3 and Ube4b [13] Knowing which proteins ... 000 g at °C Protein concentrations were determined by the Bio-Rad protein assay (Bio-Rad, Hemel Hempstead, UK) FLAG-tagged proteins were immunoprecipitated from equal amounts of total protein by ... on mislocalized VCP, and the fact that Hrd1 is a multispanning ER membrane protein that also interacts with other VCP-binding ER proteins [24] makes it difficult to confirm direct binding by coimmunoprecipitation...

Ngày tải lên: 18/02/2014, 13:20

... enhanced green fluorescent protein (GFP) Therefore, the adenovirus used to express MCD protein also expressed GFP, which served as a marker of successful viral infection and protein overexpression ... and whole cell lysates were used for MCD assay Protein concentrations of the cell lysates were determined by a Bradford protein assay kit Subcelluar fractionation to isolate cytosol and mitochondrial ... isoform of MCD protein was not statistically significant Despite the increased expression of MCD protein, MCD activity in cytoplasmic fraction (normalized to milligrams of total protein) was not...

Ngày tải lên: 16/03/2014, 16:20

... variable in the field, and on the average considerably lower than on the other experimental scales (Strômberg, 1997); and (e) water flow patterns, ranging from total mixing and no flow in the batch ... mixing and no flow in the batch experiments, via homogeneous flow in the column experiments, to existence of preferential flow paths in the field (Eriksson & Destouni, 1997; Eriksson et al., 1997) ... Pyr (Pyrite), respectively Assessing mine water pollution: from laboratory to field scale 311 magnitude of the discrepancy between laboratory rates and rates at the field scale, however, is similar...

Ngày tải lên: 23/03/2014, 04:20

... order to develop DEM at this scale, it requested fields data to be compatible In here, fields data are the topographical map with equivalent scale and field survey data In the DEM development from ... rural and urban area, rice and vegetables field, aquacalture ponds and infrastructures In the zone and 2, the aquacalture ponds, rice and vegetables field is mainly Flood can be divided into ... However, by using satellite data to calculate parameters in the first group is quickly, cheapper and bigger calculated area comparing other method CONCLUSION - A method of detecting and assessing...

Ngày tải lên: 26/03/2014, 14:07

... [3–7] It has been extensively studied using genetic approaches, and a large number of mutational variants affected in their protein RNA and protein protein interactions have been produced [8,9] ... coat protein and RNA has been investigated [5,9,23,24], and amino acid–nucleotide interactions contributing to its stability have been characterized [13,25], but the role of protein protein and protein RNA ... protein RNA interactions in virus stability is not completely understood, and we have investigated these questions in this work We sought to determine the role of protein RNA and protein protein interactions...

Ngày tải lên: 30/03/2014, 11:20

... relevant system in which to examine a-synuclein–lipid interactions In order to distinguish a-synuclein–lipid interactions from a-synuclein protein interactions, synaptosomal lipids were isolated by ... lysosomal and Golgi membranes in this preparation Our experiments address only synaptosomal membrane interactions and therefore represent a small population within the whole cell membrane preparation ... lipid interface were analyzed as a measure of protein membrane interactions To obtain further insight into the mechanism of a-synuclein–bilayer interactions, laurdan fluorescence spectroscopy...

Ngày tải lên: 30/03/2014, 15:20

... membranes than to the zwitterionic membrane, as seen by ITC Therefore, their microbial specificity is likely a consequence of improved electrostatic interactions with microbial vs eukaryotic membranes ... positive membrane curvature However, not all lytic peptides promote positive membrane curvature For example, the peptide mastoparan, isolated from wasp venom, has the opposite effect on membrane ... similar activity against liposomes when bound to the membrane, its activity against biological membranes can be altered by its ability to access the membrane surface [34] Both b-17 and [Ala8,13,18]-magainin...

Ngày tải lên: 31/03/2014, 07:20

... levels in bacterial cells using flow field-flow fractionation Anal Biochem 313(1):76–85 Chapter Assessing Protein- Ultrafiltration Membrane Interactions Using Flow Field -Flow Fractionation Galina E ... Contents Flow FFF – Basics and Key Applications Karl-Gustav Wahlund and Lars Nilsson Assessing Protein- Ultrafiltration Membrane Interactions Using Flow Field -Flow Fractionation ... Dukjin Kang Field -Flow Fractionation in Therapeutic Protein Development 73 Joey Pollastrini, Linda O Narhi, Yijia Jiang, and Shawn Cao Assessing and Improving Asymmetric Flow Field -Flow Fractionation...

Ngày tải lên: 29/05/2014, 16:16

... Name Introduction of the principles of GAP for citrus through implementation of citrus IPM using Farmer Field Schools Vietnamese Institution Ministry of Agriculture and Rural Development, Plant ... Practice on the field and discussion - Group dynamic/team building activities - Ago-Ecosystem analysis - Citrus in building phase (Nutrition requirement after harvesting and before flowering stage) ... • provide record keeping materials • limit field studies to those that are specific to province/ community needs • understand the appeal of farmers using cheap illegal Chinese pesticides Participants...

Ngày tải lên: 21/06/2014, 04:20

... in two ways: at the beginning and at the end of the project (B&E) using similar methodology used in project 036/04 VIE and b) using continuing monitoring of two groups of farmers: FFS group and ... the FFS However, this limitation will be minimised by using large sample sizes and triangulation: KAP surveys, semi-structured interviews and field observations The baseline study performed in June ... assessed The baseline study was undertaken by combining observations made in the field, interviews with farmers in the field, surveys of FFS participants, interviews and surveys of trainers, interviews...

Ngày tải lên: 21/06/2014, 04:20

... Recognize major stages in plant and fruit development like major flushing, flowering, fruit set etc Manipulate flushing, flowering and harvest time to optimize production Apply fertiliser at right ... yield is probably due to a combination of factors: poor pollination, wet and cold weather during flowering period, age of trees, inadequate fertilisation and diseases 1.1.2 Orchard layout and growth ... monitoring of pests and beneficials, respectively The majority of farmers in Northern Central Provinces using baits for control of fruit fly (85% increased from 40%) but only 23% of farmers used bait...

Ngày tải lên: 21/06/2014, 05:20

... cycle: After harvest to flowering Flowering, fruit-set, initial fruit grow Fruit grow and ripening Harvest Presentation and discussion Activity 2: Drafting of curriculum for 2009 Using the tables developed ... farmers and Metro provided funds for certification 4.1.4 Pest and Diseases Field Guide for North of Viet Nam Pest and Diseases Field Guide for North of Viet Nam was printed and distributed to PPD staff, ... Title: Introduction of the principles of GAP for citrus trough implementation of citrus IPM using Farm Field School Vietnamese Implementing Institution: Plant Protection Department Narrative OBJECTIVES...

Ngày tải lên: 21/06/2014, 05:20

... Title: Introduction of the principles of GAP for citrus trough implementation of citrus IPM using Farm Field School Vietnamese Implementing Institution: Plant Protection Department Narrative OBJECTIVES ... understandable and easy for farmers to apply Similarly, interactions between farmers, extension officers and scientists resulted in the publication of two field guides for pests, diseases and nutrient ... extent, farmers was much more balanced and flowing equally between partners Communication between the Australian and Vietnamese partners, however, still mainly flowed in one direction, with the Australians...

Ngày tải lên: 21/06/2014, 05:20

... Name Introduction of the principles of GAP for citrus through implementation of citrus IPM using Farmer Field Schools Vietnamese Institution Ministry of Agriculture and Rural Development, Plant ... trained Out of the 3000 farmers, 2,451 have been trained using AusAID CARD funds and approximately 540 additional farmers have been trained using provincial funds Farmers increased their knowledge ... the Field Guide were printed by Agricultural Press in Hanoi and distributed to PPSDs, DARDs, trainers and farmers in the Northern provinces Farmers and trainers from the Mekong delta used Field...

Ngày tải lên: 21/06/2014, 05:20

... cross-checked with field observations and farmer records Data presented for compliance with GAP requirements are the result of discussions with farmers, their trainers and from field inspections ... category, in addition to using pesticide not specifically registered for citrus, another difficulty is meeting pre-harvest interval requirements, because continuous flowering and fruiting throughout ... fruit profitability Fruit drop after flowering Very low Yes Not stated Not stated Not stated yield Fruit drop after Yes Not stated Not stated Not stated flowering Very low yield Fruit drop after...

Ngày tải lên: 21/06/2014, 05:20