assay elisa and flow cytometry

... described before [35] ELISPOT assay, ELISA, and flow cytometry The ELISPOT assay used for detection of IFN-γ secretion by CD8+ T cells was modified from Larsson et al [25] and previously described ... was undetectable and remained so throughout a year of follow-up Several blips of viremia occurred, and analyses of both viral RNA and DNA demonstrated persistence of nef deletions and pol sequences ... SIV-specific antibodies and CD8+ T cell responses were evaluated in both tenofovir-treated and control macaques before and after challenge with SIVmac055 Antibodies to both SIV gp130 and p27 antigens...

Ngày tải lên: 13/08/2014, 09:20

... Cycle and Cell Death Analysis The effects on the cell viability and the cell cycle distribution were determined by DNA flow cytometry Flow cytometric analyses were performed using a PAS II flow ... viability and apoptotic cells were assessed by flow cytometry with propidium iodide (PI)-method For detection of apoptotic cells and viability a FACS Calibur flow cytometer (Becton Dickinson Cytometry ... decisions High resolution flow cytometry is an appropriate tool not restricted to the monitoring of the therapeutic effect DNA aneuploidy, as determined with high-resolution flow cytometry, has been...

Ngày tải lên: 26/10/2012, 09:48

... specifically flow cytometry PD assay have been proposed These guidance and white papers can serve as a template or guide for the validation of a flow cytometry PD assay Flow cytometry PD assays should ... for ligand binding assays [12] as a foundation in which to base the validation of a flow cytometry pharmacodynamic assay and applying the "appropriate" parameters for a cell based cytometry assay, ... pharmacodynamic assays, particularly assays based on flow cytometry This is in contrast to other assays used in drug development such as ligand binding and immunogenicity assays [10-15] Additionally,...

Ngày tải lên: 18/06/2014, 15:20

... and flow cytomCorrelation between static platelet adhesion and flow cytometry Correlation between ADP-induced platelet adhesion (Factor 1) and ADP-induced platelet activation as measured by flow ... Figure The influence of ASA and clopidogrel on platelet activity measured by flow cytometry The influence of ASA and clopidogrel on platelet activity measured by flow cytometry The effects of platelet ... and percentage platelet adhesion was calculated Flow cytometry Platelet expression of P-selectin and binding of fibrinogen were measured by flow cytometry as indicators of platelet activation...

Ngày tải lên: 18/06/2014, 15:20

... RTH2A and RTH230A; RT21A and RTH21A; and RTH26A, RTH65A, and Ken5; recognize Pan T1, Pan T2, and Pan T4 LDM respectively Zenon second step antibodies were used to demonstrate RTH2A (IgG1) and RTH230A ... lymphocytes Flow cytometry A Becton Dickinson FACSort equipped with a MAC computer and Cell Quest software (BD Immunocytometry Systems, USA) were used to collect data Data analysis Cell Quest and FCS ... CD8 CD8 CD4 and CD8 subpopulations Basophils and subpopulation of CD4+ T lymphocytes Granulocytes, basophils, and monocytes Subpopulations of B and T lymphocytes Subpopulations of B and T lymphocytes...

Ngày tải lên: 07/08/2014, 20:23

... collected and stored at -80°C for measurement of IL-12, TNF-α and IFN-γ levels by ELISA (ebioscience, San Diego, CA, USA) The minimum limits of detection for IFN-γ, IL-12 p40 and TNF-α were 8, 80 and ... patients and controls Disease activity scores were determined before and after steroid treatment (Table 1) and the SLE patients were divided into the SR and the SS groups accordingly GR expression and ... by RLBA Analysis of GR binding in (a) K562 and (b) U937 with and without 10-6 M Dex by FITC-DexRLBA FCM and RLBA Parallel FCM-FITC-Dex and radiometric assays were performed on the same day, using...

Ngày tải lên: 09/08/2014, 14:22

... [11-13] Principle of basophil activation test by flow cytometry As flow cytometry is a valuable tool for the analysis of many different cell types and can be used to identify specific populations ... Fluo Figure Principle1of the basophil activation test by flow cytometry (triple staining) Principle of the basophil activation test by flow cytometry (triple staining) Basophils are identified on ... FITC-CRTH2 PE-CD203c Figure Identification of CRTH2 expressing cells by flow cytometry Identification of CRTH2 expressing cells by flow cytometry Left histogram : ungated leukocytes biparametric representation...

Ngày tải lên: 13/08/2014, 13:22

... apoptosis assay are CIDE3 (positive) and CDK (negative) (b) Controls for the proliferation assay are cyclin A (positive) and YFP (negative) (c) Controls for the MAPK assay are DUSP10 (positive) and ... cell-based assays to novel tumor relevant modulators of the cell cycle Cancer Res 2005, 65:7733-7742 Bonetta L: Flow cytometry smaller and better Nat Methods 2005, 2:785-795 Tree Star Inc: FlowJo Ashland, ... Positive and negative controls for the proliferation assay were vectors expressing cyclin A and YFP, respectively In the MAPK assay, overexpression of DUSP10 was used as a positive control, and overexpression...

Ngày tải lên: 14/08/2014, 17:22

... manifolds runners and inside cylinder In the first area, the flow is unstable view the incompatibility of air and propane molecules speed In the second area, the blend was expanded (the flow instability ... 2010 [11] Fuchs T R and C J Rutland Intake Flow Effects on Combustion and Emissions in a Diesel Engine, SAE N° 980508, 1998 [12] Ugur Kesgin, Study on the design of inlet and exhaust system of ... aims at the 3D numerical analysis of flow in two manifold geometries using a CFD code FloWorks is chosen for its capabilities flow analysis for both in gas and liquid simultaneously 3.1 3D geometries...

Ngày tải lên: 05/09/2013, 16:30

... permeabilized and stained with mAbs directed against IL-1 and CCR5, IL-2 and CCR5, IFN and CCR5, IL-4 and CCR4, or IL-10 and CCR4 and were analyzed on a flow cytometer A, B, C, D, and E FACScan ... with flow cytometry- basad assays for measuring changes in cells surface Clinical Flow Cytometry Emerging Applications markers and assays for measuring the expression of intracellular cytokine Flow ... Anti-Tumoural and Immune Modulatory Activity of Natural Products by Flow Cytometry 91 Susana Fiorentino, Claudia Urueủa, Sandra Quijano, Sandra Paola Santander, John Fredy Hernandez and Claudia...

Ngày tải lên: 08/03/2014, 19:20

... Nature 171, 37–38 Flow Cytometry: An Introduction 31 46 Kachel, V., Fellner-Feldegg, H., and Menke, E (1990) Hydrodynamic properties of flow cytometry instruments, in Flow Cytometry and Sorting, 2nd ... R., Lindmo, T., and Mendelsohn, M L., eds.), Wiley-Liss, New York, pp 27–44 47 Pinkel, D and Stovel, R (1985) Flow chambers and sample handling, in Flow Cytometry: Instrumentation and Data Analysis ... Cytometry 10, 229–241 32 Gray, J W and Cram, L S (1990) Flow karyotyping and chromosome sorting, in Flow Cytometry and Sorting, 2nd edit (Melamed, M R., Lindmo, T., and Mendelsohn, M L., eds.), Wiley-Liss,...

Ngày tải lên: 08/04/2014, 12:51

... has made flow cytometry very popular and wtdely used The applications of flow cytometry are diverse and include the mterrogatlon of membrane, cytoplasmic, and nuclear antigens Flow cytometry ... York Melamed, M., Lmdro, T., and Mendelsohn, M., eds (1990) Flow Cytometry and Cell Sortzng, 2nd Ed Wiley-Liss, New York Parks, D and Herzenberg, L (1989) Flow cytometry and fluorescence-activated ... Practical Flow Cytometry 3rd ed LISS, New York Owens, M A and Loken, M R (1995) Flow Cytometric Prwczples for Clwucal Laboratory Practice Wiley-Llss, New York Radbruch, A., ed (1992) Flow Cytometry and...

Ngày tải lên: 11/04/2014, 09:41

... use of groove and flow and their interaction with the content of the text: The rhythmic styles of MCing, or “flows,” are among the central aspects of rap production and reception, and any discussion ... structural levels and in many different ways and then manipulates that flow throughout the course of the song Chapter is an analysis of “Golden Lady” that demonstrates groove and flow operating ... progressions and voice-leading to create flow Existing discussions of repetition and flow, by authors such as James Snead, John Miller Chernoff, Christopher Small, Dick Hebdidge, Tricia Rose, and Adam...

Ngày tải lên: 29/05/2014, 21:49

... and AR performed the research JCS and WM contributed vital analytical tools JCS, LW, AR, BZ, and EBW analyzed and interpreted the data JCS and EBW wrote the manuscript All authors have read and ... http://www.translational-medicine.com/content/8/1/106 Page of 15 Table Representative input and output for the “Expander” program analytical software such as FlowJo (http://www.flowjo com) and FCS Express (http://www.denovosoftware com) ... therapies [11] Thus, the use of higherorder flow cytometry and comprehensive multiparameter data analysis could facilitate the identification and expansion of T CM and T CM precursor subpopulations (i.e...

Ngày tải lên: 18/06/2014, 16:20

... permeabilized and stained with mAbs directed against IL-1 and CCR5, IL-2 and CCR5, IFN and CCR5, IL-4 and CCR4, or IL-10 and CCR4 and were analyzed on a flow cytometer A, B, C, D, and E FACScan ... with flow cytometry- basad assays for measuring changes in cells surface Clinical Flow Cytometry Emerging Applications markers and assays for measuring the expression of intracellular cytokine Flow ... Anti-Tumoural and Immune Modulatory Activity of Natural Products by Flow Cytometry 91 Susana Fiorentino, Claudia Urueủa, Sandra Quijano, Sandra Paola Santander, John Fredy Hernandez and Claudia...

Ngày tải lên: 28/06/2014, 15:20

... sensitivities of the AhFibA and anti-CCP2 assay between populations and Scatter plot: AhFibA assay versus anti-CCP2 assay in population Ab, assay versus anti-CCP2 assay in population antibody; ... value of the AhFibA ELISA and the anti-CCP2 ELISA, and conclude that both assays perform equally well, which is reflected by similar ROC curves and similar sensitivities and specificities There ... with the results of RF and five ACPA assays: anti-CCP1, anti-CCP2, anti-pepA, anti-pepB and AhFibA (Figure 3) Table Sensitivities and specificities of AhFibA and anti-CCP2 assay in the two populations...

Ngày tải lên: 09/08/2014, 08:22

... transport and consumption variables, norepinephrine and milrinone requirements, as well as pH and arterial lactate concentrations were documented immediately before and hour after randomization ... first case report and our findings, a dramatic decrease in small vessel numbers and, ultimately, a complete standstill in sublingual capillary flow occurred In their patient, Boerma and colleagues ... immediately before and hour after randomization For flowmotion analysis, the Doppler signal tracing was divided into nine blocks of 20 seconds each before induction of ischaemia and during reperfusion...

Ngày tải lên: 12/08/2014, 23:22

... Authors' contributions SC and PB conceived the study, performed the experiments and wrote the manuscript, AV and RO assisted in the flow cytometry setting and handling, MDG and PS participated in ... by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives Clin Mol Allergy 2005, 3:9 Triggiani M, Marone G: Basophil's secrets revealed by flow cytometry ... purposes) Clinical and Molecular Allergy 2008, 6:12 FITC as above Samples were reconstituted in BD-Isoflow PBS balanced buffer and read Cytometer and fluorochrome setting Flow analysis was performed...

Ngày tải lên: 13/08/2014, 13:22

Ngày tải lên: 13/08/2014, 21:21

... Results and discussion To verify the fluid flow simulation, the results of the flow simulations are compared with the exact solution for laminar pipe flow regime The exact solution for the laminar flow ... under the fully developed laminar flow regime The deposition efficiency is calculated for different flow rates, various tube lengths and different particle diameters, and the results are compared ... different lengths of 1, and cm and a mean velocity of m/s Figures 8-10 display the deposition efficiency for a pipe with the length of 5cm and different mean velocity of 0.5, and m/s in compare with...

Ngày tải lên: 09/09/2015, 10:06