ascites production of monoclonal antibodies mabs
Báo cáo khoa học: "Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf)" ppt
... syassa tolbonummi rof desu saw leg rehto eht dna )A( GBC htiw deniats saw leg eno ,ED-2 retfA EGAP-SDS %5.21 yb dewollof ,)mc ,7-4 Hp( pirts yrd GPI gnisu gnisucof cirtceleosi yb demrofrep saw ED-2 ... deifirup gI hsifkcor htiw syassa tolbonummi ni niahc thgil eht rof cificeps erew )6-7C9R ,1-4H11R( senolc dna niahc yvaeh eht rof cificeps erew )cte ,9-4B7R ,8-4B7R( senolc 91 ,sbAM eht fO senolc ... secnerefeR noitadnuoF gnireenignE & ecneicS aeroK eht fo margorP hcraeseR cisaB eht morf )0-24200 -000-1002-10R( tnarg a yb detroppus saw krow sihT stnemgdelwonkcA esu larutluciram rof seniccav dna...
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Báo cáo sinh học: "Regulatable systemic production of monoclonal antibodies by in vivo muscle electroporation" docx
... repeated injections of massive doses of antibodies and (iii) the restrictive costs of in vitro produced proteins certified for human use Moreover, injection of massive doses of mAbs results in great ... bioavaibility of these therapeutic agents that are often detrimental to the efficacy of treatments It is, therefore, important to investigate whether in vivo production of therapeutic antibodies ... neurodegenerative disease upon production of a neutralizing mAb [10] thereby demonstrating the therapeutical interest of the approach Neutralizing anti-HIV and anti-HCV antibodies, some of which are currently...
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Báo cáo khoa học: Functional characterization of the maltose ATP-binding-cassette transporter of Salmonella typhimurium by means of monoclonal antibodies directed against the MalK subunit pot
... RESULTS Monoclonal antibodies recognize epitopes in the N-terminal (ATPase) domain and in the C-terminal domain of MalK, respectively Monoclonal antibodies were prepared against the MalK subunit of ... differ from those of soluble MalK Fab fragments of 5B5 and 4H12 only slightly inhibit ATPase activity of MalK and of proteoliposomes Having established the binding properties of the mAbs, we analyzed ... ratios of 1.5 : and : 1, respectively (molecular mass of MalK, 40 kDa; molecular mass of complex, 171 kDa) In the case of proteoliposomes, this actually corresponds to a 12-fold molar excess of mAbs...
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Báo cáo khoa học: " Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules" ppsx
... level of expression of the LDM detected with MRB102A on lymphocytes was higher than Monoclonal antibodies specific for rabbit leukocyte differentiation molecules Table Reactivity of monoclonal antibodies ... plot profiles of peripheral blood leukocytes labeled with the mAbs indicated A single representative profile is shown for mAbs that recognize the same or different epitopes on the same subset of ... containing or mAbs and incubated as described Following centrifugation and cycles of washing, the lymphocytes were incubated with second step reagents For most of the studies, combinations of mAbs of different...
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Báo cáo y học: "Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA" pptx
... Epitope mapping of other monoclonal antibodies to recombinant N protein or phage library screening of infected swine sera did not identify the C terminus of N protein as a site of antibody recognition ... Epitope mapping of 1A11 C1 and 2B10 p4 To determine the linear epitope of monoclonal antibody 1A11 C1, 24 or 25-mers of non-overlapping peptides spanning over the entire N protein sequence of NiV were ... pool of NiV sero-positive swine sera from the 1999 Malaysia outbreak (Figure 3) The overall pattern of shared epitopes among polyclonal antibodies could be observed regardless of the source of...
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Báo cáo y học: "Characterization of monoclonal antibodies against Muscovy duck reovirus σB protein" ppsx
... linear range of absorbance This offers an estimation of the relative avidity of MAbs for their binding proteins Binding degrees of MAbs to HisσB using ELISA titration indicated that all MAbs saturated ... His-σB protein of DRV could induce the production of MAbs After screening and subcloning, the four MAbs directed against His-σB were isolated and characterized Analysis of immunofluorescence assay ... affinity column of protein G-agarose (Boehringer Mannheim) Antibodies were coupled to HRP by the periodate method [19] and stored at -20°C Determination of MAbs titers The titres of MAbs were determined...
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Tài liệu Báo cáo khoa học: Mapping the functional domains of human transcobalamin using monoclonal antibodies pptx
... have described a number of TC monoclonal antibodies (mAbs) that interfere with the physiological functions of human TC, i.e the Cbl and receptor binding [16] Therefore, a map of the corresponding ... of the disulde bonds prior to electrophoresis abolished the binding of mAbs 3C4 and 5H2 (Fig 2, see the corresponding lanes) Binding of antibodies to CNBr peptides on western blot Treatment of ... on the alignment of peptide fragments and their reactivity with mAbs, epitope clusters 2, and 3891 Mapping of transcobalamin using antibodies S N Fedosov et al Fig Binding of antibodies to the...
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Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies docx
... developed Two antibodies showed clear recognition of plasma purified C4BP, MoAb 15 and 44 We therefore decided to proceed with more detailed analysis of these two antibodies All monoclonal antibodies ... measured and the concentration of antibody was calculated using an extinction coefficient (1%, cm) of 12.5 Antibodies were stored at °C after addition of 0.02% NaN3 Antibodies were tested using ... elucidate the influence of CCP2 on protein S binding, we made the effort to raise monoclonal antibodies against CCP1-2 with the aim to use them in structure–function analysis Two of 12 antibodies, MoAb...
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Báo cáo khoa học: Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated a subunit of the heterotrimeric GTPase Gq potx
... Enzymatic actions of P multocida toxin Results Analysis of enzymatic actions of PMT with Gaq Q209E-specific monoclonal antibodies According to a previous study [16], the deamidation of Ga by PMT results ... characteristics of PMT have not been analyzed as a result of the lack of an easily-administered assay to detect activity of the toxin In the present study, we developed rat monoclonal antibodies that ... Fig Isolation of Gaq Q209E-specific antibodies (A) Alignment of amino acid sequences of the switch region in the a subunits of mouse heterotrimeric GTPases by CLUSTALW Sequences of synthetic oligopeptides...
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Báo cáo khoa học: Production of a recombinant mouse monoclonal antibody in transgenic silkworm cocoons pptx
... feasibility of using transgenic silkworms for the mass production of recombinant mAbs Silkworms have been used for the Production of mouse mAb by transgenic silkworms manufacture of silk in the ... proteins The absence of a-1,6-fucose comprises yet another reason supporting the use of this system in the production of mAbs The absence of a-1,6-fucose enhances the activity of antibody-dependent ... Percentage of H-chain to sercin-1 0.0 4.4 ± 0.7 2.9 ± 0.1 a Copy numbers of mRNAs of L-chain and H-chains of the recombinant IgG, and sericin-1 per 10 ng of total RNA The indicated values are 10)5 of...
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báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... and probed with a panel of anti-MUC16 monoclonal antibodies Arrows indicate migration of 250 kDa molecular weight marker and identity of antibody used is shown on the right of each blot We have ... CA125 in spent media of MOVCAR-2 cells We therefore tested a panel of ten anti-MUC16 antibodies that we recently generated using the novel ABL-MYC technology All of these antibodies were able ... weight of MUC16 requires the use of extensive molecular biological approaches to study the importance of this mucin in the pathogenesis of ovarian cancer In addition, a thorough study of MUC16...
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Báo cáo toán học: " Inert coupling of IRDye800CW to monoclonal antibodies for clinical optical imaging of tumor targets" potx
... and to tailor therapy with existing mAbs by providing information about the targeting performance of mAbs and the expression status of cell surface targets The mAbs labeled with radionuclides can ... coupling of IRDye800CW to mAbs, enabling clinical PID as a complementary tool to radioimmunodetection In the present study, we evaluated the impact of the coupling of different numbers of IRDye800CW ... impairment of mAb biodistribution characteristics might occur The mAbs with eq of IRDye800CW coupled showed clear tumor delineation by optical imaging The use of IRDye800CW-labeled mAbs and antibodylike...
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Báo cáo khoa học: "The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis)" pdf
... use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis) development and analysis of species specific and cross reactive monoclonal antibodies ... Naessens, J Analysis of monoclonal antibodies specific for the γδ TcR Vet Immunol Immunopathol 1996, 52, 275-283 Davis, W C., Davis, J E and Hamilton, M J 1995 Use of monoclonal antibodies and flow ... of over 200 mAbs yielded 138 with patterns of reactivity similar or identical to those in cattle The use of crossreactive monoclonal antibodies to characterize the immune system of the water...
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Báo cáo khoa học: "Characterization of Antigenic Sites on the Rinderpest Virus N Protein Using Monoclonal Antibodies" docx
... with 50 μl of serial dilutions of un-labeled competing Mabs for 30 at 37℃ Without wash step, an equal volume of the biotinylated Mab of maximum absorbance was added into all wells of the plates ... deviations (SD) of nine negative control sera Results P ro du c tion a n d c h arac te rizatio n of N p rote in sp e c ific Mabs A total of 10 Mabs were raised against N protein of RPV-LATC strain ... R-8C-04 (Kd = 0.33 μg/ml) The affinity of other Mabs was high (Kd
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Báo cáo y học: "Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia" ppt
... prepare the anti-NPM-mA monoclonal antibodies (mAbs) as the primary antibody in IHC assay In 1999, Cordell et al prepared the first panel of mAbs associated with NPM1 protein, two of which recognized ... detection of cytoplasmic dislocation of NPM1 by IHC has been performed using aspecific antibodies that bind both the NPM-wt and NPM-mA proteins In IHC assay labeling with this kind of mAbs, the ... localization of NPM1 may not be closely associated with NPM1 gene mutations probably because of NPM1 diffusion during the tissue fixation and the influence of fixatives [20] Thus, production of anti-NPM-mA...
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Báo cáo y học: "Differential clinical efficacy of anti-CD4 monoclonal antibodies in rat adjuvant arthritis is paralleled by differential influence on κ α NF-κB binding activity and TNF-α secretion of T cell" pot
... models of arthritis, therefore representing potential therapeutic targets [1] A specific therapeutic approach is the direct targeting of CD4+ T cells by use of monoclonal antibodies (mAbs) against ... sake of simplicity, we refer to the mAb RIB5/2 as accelerating (although this term is applicable only to the onset of AA) and the mAbs W3/25 and OX35 as ameliorating Affinity of the monoclonal antibodies ... study shows differential clinical efficacy of three anti-CD4 mAbs in the preventive treatment of AA The lack of differential clinical efficacy of the same mAbs in established AA may be explained...
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Báo cáo y học: "The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin" ppsx
... hybrid VL chain construct contained the CDR1 of one of the human monoclonal IgG antibodies IS4, B3 or UK4 and the CDR2 and CDR3 of a different one of these antibodies Two hybrid VL chains (BU and ... in most, but not in all, sequences of pathogenic monoclonal aPL From our detailed analysis of all published sequences of monoclonal aPL we found that of 13 monoclonal aPL that had been examined ... vitro expression of whole IgG molecules from cloned VH and VL sequences of human monoclonal aPL antibodies [29] This system was used to test the binding properties of combinations of heavy chains...
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Báo cáo y học: "Arthrogenicity of type II collagen monoclonal antibodies associated with complement activation and antigen affinity" pps
... Values for the activation of C3 by mAbs were expressed as a percentage of the CII-3 value (800 μg/ml) Cross-reaction of mAbs with mouse or chicken CII The cross-reaction of mAbs (CII-6, C2A-12, CII-3, ... http://www.journal-inflammation.com/content/8/1/31 Page of Figure Effect of an extra monoclonal antibody on the arthritogenicity of the 3-clone cocktail (CII-3, C2B-14, and CII-6) A: DBA/1J mice were given i.p injections of a cocktail of CII-3, ... followed by an i.p injection of LPS (50 μg/mouse) on day The mice were observed daily after the injection of mAbs for the development of arthritis until day 10 The severity of arthritis was scored...
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Báo cáo y học: "Arthrogenicity of type II collagen monoclonal antibodies associated with complement activation and antigen affini" doc
... Values for the activation of C3 by mAbs were expressed as a percentage of the CII-3 value (800 μg/ml) Cross-reaction of mAbs with mouse or chicken CII The cross-reaction of mAbs (CII-6, C2A-12, CII-3, ... http://www.journal-inflammation.com/content/8/1/31 Page of Figure Effect of an extra monoclonal antibody on the arthritogenicity of the 3-clone cocktail (CII-3, C2B-14, and CII-6) A: DBA/1J mice were given i.p injections of a cocktail of CII-3, ... followed by an i.p injection of LPS (50 μg/mouse) on day The mice were observed daily after the injection of mAbs for the development of arthritis until day 10 The severity of arthritis was scored...
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Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
... cytokine production in both human PBMCs and mouse splenocytes in vitro [19] In this report, we detail the generation and selection of fully human monoclonal antibodies (HuMAbs) specific for Page of ... SEB [11-14] The use of mouse monoclonal anti-SEB antibodies to study important epitope determinants essential for MHC/TCR binding has led others to explore the use of anti-SEB antibodies for blocking ... flow rate of 100 μl/min The on- (ka) and off-rate (kd) of HuMAbs were determined by observing the signal over time for triplicate injections at each concentration above Blank injections of HBS-EP...
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