as in subheading gene expression

... Medicine 2009, 7:30 Background There is increasing interest in knowledge about the impact of intrauterine development during adult life [1] Low growth rate in fetal life is associated with increased ... aim has been obtaining the four vascular cell types from each individual UC to determine their cellular and molecular properties, as both ECs and SMCs are important in maintaining the vascular ... intrauterine growth retardation Whether or not changes in BP in low birth weight subjects may result from vascular imprinting with early changes in cells from the vascular wall is an intriguing...

Ngày tải lên: 18/06/2014, 15:20

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Ngày tải lên: 07/08/2014, 18:21

... various inflammatory cytokine proteins involved in the peak inflammatory reactions induced by ALI with E coli instillation Protein levels of inflammatory cytokines from lung homogenates, including ... MSCs was evidenced by decreased injury scores at post-injury day and attenuated lung inflammation, including a reduction in MPO activity and inflammatory cytokine protein levels, at postinjury ... cytokines in lung homogenates, and increased water content in the lungs corresponding to an increased wet-dry lung ratio that peaked at post-injury day Furthermore, the mortality rate in E coli-induced...

Ngày tải lên: 12/08/2014, 13:22

... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C ... noitartsinimda suonevartnI M ppohC ,J somaR -zehcnaS ,L gnaW ,Y iL ,A doomhaM ,RP grebnaS ,D uL 02 49-98 ,8 ,7002 icS teV J noisserpmoc noollab larudipe yb decudni ledom yrujni droc lanips eninac ... sCSM-BCU ehT ]62[ noitartnecnoc 2OC %5 dna Co73 ta )ASU ,LRB -ocbiG( nicymotperts-nillicinep %3.0 dna ])ASU ,LRB -ocbiG( enimatulg-L l/lomm htiw )ASU ,LRB-ocbiG( mures enivob latef %01 fo noitidda...

Ngày tải lên: 07/08/2014, 23:22

... antibody-based protein array 392 Byung-Jun Jang et al and enzyme-linked immunosorbant assay indicated that bone marrow MSCs secrete distinctively different cytokines and chemokines such as vascular ... by canine UCB-MSCs In a cortical allograft, the host-graft interface heals within months [11] Vascular invasion begins at the interface and progresses toward the center of the graft Remodeling ... Fu WM Basic fibroblast growth factor stimulates fibronectin expression through phospholipase C gamma, protein kinase C alpha, c-Src, NF-kappaB, and p300 pathway in osteoblasts J Cell Physiol...

Ngày tải lên: 07/08/2014, 23:22

... differentiation was evaluated by calcium mineralization Alizarin red S staining was used to determine the presence of calcium mineralization For Alizarin red S staining, cells were washed with D.W ... show a consistently increasing growth rate during the passages Each bar increase originates from the CPDL cumulative values, which were two separated sampled cells B: Phase-contrast image of cUCB-MSCs, ... (Fig 3A) Alizarin red staining was positive after weeks under osteogenic induction media (Fig 3B) Also, gene expression of markers associated with osteoblastic differentiation such as MSX2, COL1A1,...

Ngày tải lên: 07/08/2014, 23:22

... and toluidine blue staining Scoring of slides from immunohistochemical staining of cellsurface markers, ECM proteins and toluidine blue staining of total proteoglycans was performed blinded by ... [21,22] Immediately after euthanasia, adipose tissue from the back and inguinal areas was surgically obtained using sterile techniques, placed in a petri dish containing Hank's Buffered Salt Solution ... activated protein kinase Synergistic interactions between TGFβ and other cytokines, such as IGF1, has been reported [16] The IGF-1-activated signalling cascade is hypothesised to interact with...

Ngày tải lên: 09/08/2014, 10:23

... differentiation of hADSCs Compared to non-induced control (A), Alkaline phosphatase staining was increased after being induced for weeks (B) (Bars = 100 μm) and ALP activity was quantified by PNPP analysis ... plateau phase According to the Patterson formula, the doubling time in the log phase of the 3rd passage was 24.8 hours There was no significant difference in the growth rate among different passages ... were cultured in osteogenic medium for weeks, osteoblast-like cells could be clearly demonstrated by alkaline phosphatase (ALP) staining (Figure 5A, B) and ALP activity was increased as shown by...

Ngày tải lên: 10/08/2014, 10:20

... modulating specific gene expression, the mRNA expression of other specific genes involved in adipogenesis regulation was analyzed The early steps of differentiation are linked to activation Gibellini ... gp120) mRNAs at day As expected, the pre-treatment of HIV-1 strains or gp120 with anti-gp120 pAb inhibited the specific mRNA increase In parallel, some late adipogenetic markers such as adipsin and ... homeostasis [84-86] and may play a role in the linkage between HIV infection and cardiovascular disease [9] HIV-related chronic inflammation determines the expression of several cytokines especially...

Ngày tải lên: 13/08/2014, 01:20

... potential in improving pulmonary functions in various settings of lung diseases, including acute lung injury [19-23] In addition to regulating angiogenic [24] and pro-inflammatory [25,26] cytokines associated ... formalin before embedding in paraffin and the tissue was sectioned at μm for light microscopic analysis After hematoxylin and eosin (H & E) staining, the number of alveolar sacs was determined in ... was defined as the region of thickened septa in lung parenchyma associated with partial or complete collapse of alveoli on H & Estained sections, was determined in a blinded fashion The scoring...

Ngày tải lên: 18/06/2014, 22:20

... blot assay As can be seen in figure 6, the expression of VEGF significantly increased in the simvastatin group than in the control group (p < 0.05) Moreover, the expression of VEGF was higher in ... concentration of simvastatin increased in vitro This indicated that the simvastatin has a biphasic dose-dependent effect on angiogenesis in vitro Weis et al previously demonstrated that statins have proangiogenic ... saline injection (control group), (2) simvastatin administration plus saline injection (simvastatin group), (3) vehicle administration plus MSCs transplantation (MSCs group), (4) simvastatin...

Ngày tải lên: 10/08/2014, 05:21

... Temporal expression patterns of various signaling molecules involved in fracture healing Dashed lines represent differences in opinions by the scientists in terms of the timing of expression (Reprinted ... Alkaline phosphatase (ALP) expression 107 4.2.6 Protein assay 107 4.2.7 Immunostaining 108 4.2.7.1 CD29 staining of BM-MSCs 108 4.2.7.2 Osteocalcin (OC) expression ... Haematoxylin and Eosin (H&E) Staining Method Appendix C: Masson Trichrome Staining Method Appendix D: Von Kossa Staining Method XV List of Abbreviations Ab: Antibody ALP: Alkaline phosphatase AFM:...

Ngày tải lên: 11/09/2015, 09:57

... cells are being investigated in a myriad of preclinical disease models [18,19,24,25] The safety of CBMC transplantation has been investigated in several human clinical trials with neurodegenerative ... >50% was deemed to be markedly effective; 5%-49% was classified as effective, while

Ngày tải lên: 18/06/2014, 19:20

... cells are being investigated in a myriad of preclinical disease models [18,19,24,25] The safety of CBMC transplantation has been investigated in several human clinical trials with neurodegenerative ... >50% was deemed to be markedly effective; 5%-49% was classified as effective, while

Ngày tải lên: 20/06/2014, 03:20

... heterotrimeric G proteins and intracellularly by undefined mechanisms Myocardial tissue engineering has now emerged as a promising treatment for heart diseases such as severe heart failure As a new transplantation ... findings provide a potential role for S1P in causing cardiomyocyte induction of HUMSCs under in vivo conditions and should be an exciting direction to explore in the future As demonstrated in ... Immunostaining with specific antibodies revealed that cardiomyocyte markers including myosin heavy chain (MHC) and sarcomeric aactinin were strongly expressed in differentiated myocytes in CMCM...

Ngày tải lên: 10/08/2014, 05:21

... was determined The invasive behavior of FLSs was assayed using the Cytoselect 24-Well Cell Migration and Invasion Assay (Cell Biolabs Inc, San Diego, CA, USA) according to the manufacturer’s instructions ... differentiation of UC-MSCs staining for alkaline phosphatase Arrows indicate the accumulation of intracytoplasmic alkaline phosphatase of osteoblast Original magnification × 40 involved in the suppression ... enzyme-linked immunosorbent assay; FLS: fibroblast-like synoviocyte; FOXP3: forkhead box P3; IL-10: interleukin 10; IDO: indoleamine 2,3-dioxygenase; INDO: indomethacin; L-NAME: N-nitro-Larginine...

Ngày tải lên: 12/08/2014, 15:22

... changes in STZ induced diabetic mice 163 Figure 7.7 Insulin staining of mouse pancreas……………………………………….165 Figure 7.8 Insulin staining of the CLEC cluster…………………………………… 169 Figure 7.9 GFP and insulin ... of cord lining cells in vivo using an immunocompetent mouse model To test the therapeutic application of cord lining cells in ex vivo insulin gene therapy using a streptozocin (STZ) induced type ... According to Drukker’s study, MHC I expression in human ESCs increased during differentiation, both in vitro into embryoid bodies and in vivo into 12 teratomas (Drukker et al 2002) In contrast,...

Ngày tải lên: 11/09/2015, 10:02

... amino- and carboxyl-binding antibodies After immunoblotting, PrPC proteins were detected using N- or C-terminal binding antibodies The signal intensity of each of the three protein bands was ... determining different PrPC banding patterns is a feature used especially to find heterogeneity based on protein conformation in one species [29] Independent of individual brain regions, in this ... N-terminal domain including octapeptide repeats, a central hydrophobic domain and a C-terminal region with two asparagine-linked glycosylation sites and a disulphide bond between cysteine residues...

Ngày tải lên: 19/02/2014, 02:20