artemis dna pkcs and the nuclease of nhej
Báo cáo y học: "DNA transposons and the role of recombination in mutation accumulation in Daphnia pulex" pdf
Ngày tải lên :
09/08/2014, 20:21
... higher rates of both DNA transposon gain Page of 13 and loss than their asexual counterparts We describe the general landscape of DNA transposons in D pulex, survey the relative abundance of each ... outputs from BLASTN (e-value < 0.00001 and >20% of the length of the query) and RepeatMasker (RM; >50 bp in length, >70% similarity, and >20% of the length of the query), respectively Families with ... from the unmasking of recessive, negative effects of the DNA transposon once the insert is present at the same locus on both chromosomes The second is the dramatic reduction in the probability of...
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Báo cáo y học: "All motifs are not created equal: structural properties of transcription factor - dna interactions and the inference of sequence specificity" doc
Ngày tải lên :
14/08/2014, 14:21
... ( K is the number of components in the Dirichlet mixture) and marginal distribution of the class variable at the first position The number of prior classes (the number of components in the Dirichlet ... assumptions about the information profile sought The likelihood of PWMs deviating from the specified prior are penalized based on the extent of their deviation, with the strength of the penalty under ... between the structural footprint of a transcription factor bound to DNA and the information profile of its PWM, is that if we knew (or could predict) the footprint of a transcription factor on DNA then...
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DNA by Design. Stephen Meyer and the Return of the God Hypothesis
Ngày tải lên :
01/11/2013, 07:20
... Davis, and others – not even accept the scientific dating of the Cambrian However, even the Old Earthers, such as Behe and presumably Meyer, have offered no positive account Is their view that the ... basis is only the initial part of the ID program Rejecting naturalism and any evolutionary account as a basis for the possibility of human knowledge, Meyer and other ID theorists turn to the alternative ... legacies” and the renewal of “a broadly theistic understanding of nature.”10 Thaxton and Meyer say that according to the modern view, “only man’s material complexity distinguishes him from the other...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
Ngày tải lên :
19/02/2014, 07:20
... donor and acceptor dyes, will therefore unveil the status of physical union and separation of complementary strands during renaturation and strand exchange, respectively, and thereby assesses the ... monitor the time course of renaturation and strand exchange activities of OsDmc1 The renaturation activity was found to be stimulated in the presence of ATP and was saturated at 1.25 lm of OsDmc1 ... intriguing to note that the initial rate of renaturation in the presence of ATP-c-S was similar to that of ATP, but the reaction rate suddenly plummeted after about 50 s of the reaction There is no simple...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
Ngày tải lên :
20/02/2014, 01:20
... in the overall (tertiary) structure of the protein Figure shows the fluorescence spectra of wild-type SNase and the four mutants E142O, K133A, W140A and W140O The fluorescence spectra of E142O and ... as the sum of fractional multiples of the reference spectra for each structural type Figure 1A shows the CD spectra of W140A, E142O and K133A There are no significant differences from that of the ... analysis of protein unfolding The DSC curves of the wild-type protein and the mutants K133A, E142O, W140A and W140O are shown in Fig 3, with their thermodynamic parameters summarized in Table The...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
Ngày tải lên :
07/03/2014, 21:20
... capacity (DCp) and difference of DCp of mutant from wild-type (%) were determined and the results for the wild-type protein and 20 mutants are given in Table The DHcal and Tm of the wild-type ... 33% lower than that of the wild-type protein The thermodynamic parameters of the glycine sustitution mutants also differed greatly from the wild-type protein The Tm values of these mutants ranged ... calculated based on the description of Privalov and Potekhin [6] residue substitution on the destabilization of secondary structure that leads to the large perturbation of the stability of the protein...
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Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf
Ngày tải lên :
30/03/2014, 11:20
... using two different dilutions (1 : and : 729) of template cDNA from the control clone (lanes and 3) and the same dilutions of cDNA from the KD-1 clone (lanes and 4) Control reactions with b-actin ... decreases formation of 8-oxoG in mitochondrial DNA [20], but the role of this protein in protecting nuclear DNA from oxidation in human cells and the mechanisms of regulation of the PRDX5 gene are ... [33] and mouse cells deficient in the ribonuclease Dicer [34], which is involved in processing and formation of siRNA We compared cDNAs synthesized using random primer on total RNAs from the PRDX5...
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The mammalian transcriptome and the function of non-coding DNA sequences pdf
Ngày tải lên :
09/08/2014, 20:20
... hand and the developmental complexity of organisms on the other, although the reasons for the abundance of intron sequences and their functions in higher organisms are not fully understood The ... reviews Our understanding of the cellular function of ncRNAs has expanded far beyond the initial notion of their being intermediates and accessories in protein biosynthesis The size of ncRNA molecules ... first, the transcribed, but nontranslated, part of the genome; second, the length and number of introns in protein-coding genes; third, the number and complexity of cis-control elements and the...
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báo cáo khoa học: " DNA sequence diversity and the origin of cultivated safflower (Carthamus tinctorius L.; Asteraceae)" ppsx
Ngày tải lên :
12/08/2014, 05:20
... detected by the presence of double peaks and coded following the conventions of the International Union of Biochemistry and Molecular Biology From these, haplotypes were resolved using the maximum ... native to the deserts of southern Israel and western Iraq, is the wild progenitor of safflower Safflower and C palaestinus share a self-compatible breeding system [12]; thus, the near absence of heterozygous ... stocks (Fig 2) Further investigation of the 'seven centers' hypothesis, will require the development and application of more variable markers to a much more robust sampling of the available safflower...
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Báo cáo y học: "Regulatory RNAs and the demise of ‘junk’ DNA" potx
Ngày tải lên :
14/08/2014, 17:22
... (Dictyostelium), and flatworms (planarians) The function of most of these RNAs remains unknown and awaits further experimentation, but the impact of all these studies showed us how much there is left ... physical entities such as DNA and RNA, while red text refers to activities Figure courtesy of and adapted from J Mattick Opening out the RNA world The ramifications of the behavior of some noncoding ... this is probably just the tip of the iceberg” The next few years are certain to bring new discoveries that will provide a greater appreciation and understanding of the role of RNA in regulating...
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Báo cáo sinh học: "Comparison of different delivery systems of DNA vaccination for the induction of protection against tuberculosis in mice and guinea pigs" pps
Ngày tải lên :
14/08/2014, 19:22
... VLDB and CRZB in the laboratory of CLS and the Company Farmacore Biotecnologia Ltda, who also shared their expertise in the DNA vaccine The majority of the research was done in the laboratory of ... reduction in the cellular infiltrate in the lung parenchyma of mice and guinea pigs Thus, this combination of DNA vaccine and adjuvants with immunomodulatory and carrier properties holds the potential ... concerning the safety of using live attenuated viruses that have been replaced by subunit or DNA vaccines alone In the prevention of TB, the prime-boost strategy of combining DNA priming and boosting...
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Cambridge.University.Press.Gender.Race.and.the.Writing.of.Empire.Public.Discourse.and.the.Boer.War.Sep.1999.pdf
Ngày tải lên :
21/09/2012, 10:54
... the food stocks, and the mood of the garrison The tactics of the Daily Mail captured the attention of the nation; the newspaper dramatized the situation of the town by emphasizing the danger that ... examine both the role of the press in the formation of public opinion about imperialism and the role of the press in the formation of the concept of public opinion itself Mafeking Night marks the powerful ... powerful beginning of the New Journalism at the same time as it marks the beginning of the end of the New Imperialism The coincidence of these occasions arises from the nature of the South African...
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Cambridge.University.Press.War.and.the.Law.of.Nations.A.General.History.Sep.2005.pdf
Ngày tải lên :
21/09/2012, 11:02
... of individual, hand-to-hand combat Grand strategy, on the other hand, remained the preserve of Tiw.11 In India, too, much the same phenomenon was apparent in the role of Indra, the chief of the ... the mainstream of legal analysis, largely as a result of the crumbling of older conceptions of legitimacy and the rise of new aspirations for democracy and the self-determination of peoples The ... testimony both to the warlike character of the Greeks and to their feeling of cultural unity, the cities of the Amphictyonic League of Delphi agreed that, in the event of war, the combatant states...
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Circuit theory of finance and the role of incentives in financial sector reform
Ngày tải lên :
24/10/2012, 09:33
... creating and advancing liquidity to producers; along the circuit, the financial system governs the supply and demand of funds and, at the end of the process, it determines the condition for the closure ... institutions and strengthen the complementarity between the formal and the informal sectors The poorest people of rural and urban societies live beyond the frontier of formal finance, or outside the formal ... affected by the creation and use of fiat-money Crucial to this purpose is the functional separation that the theory operates between banks and firms The macroeconomic focus of the theory, however,...
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Ensuring Financial Stability: Financial Structure and the Impact of Monetary Policy on Asset Prices
Ngày tải lên :
24/10/2012, 09:40
... zero even at the 95% level in Canada, Finland, the Netherlands, Norway, Sweden, Switzerland, the UK and the US Moreover, while in some countries, (including Finland, the UK and the US) residential ... also for these countries the existence of cointegration could not be rejected We therefore specify the VAR models in the level of the variables Nevertheless, we neither impose the number of cointegrating ... to offset the macro economic effects of the collapse of the bubble).2 Of course, it is by no means clear that central banks are better able to judge the appropriate level of asset prices and the...
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Facts of Vietnam Freight forwarding industry and the role of Vietnam Freight Forwarders Association (VIFFAS) to the industry in international economic integration processhelpful to Oristar.doc
Ngày tải lên :
27/10/2012, 16:42
... kinds: + The change of consumer-demand modal, market and the attitude to the availability and diversity of goods + The pressure of reducing cost + The development of Information technology + The pressure ... learning and expanding With their experiences, they have learned the value of offering quality services In the way of growing, they have also open branches to establish a worldwide presence These ... The Association has the role of coordinating the activities of the members in order to protect the interests of the members and improve the position of Vietnam in the international freight forwarding...
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Báo cáo y học: "The association of meat intake and the risk of type 2 diabetes may be modified by body weight"
Ngày tải lên :
31/10/2012, 16:49
... the amount consumed in lians (50g/lian) per unit of time A lian is the unit of weight in China equivalent to 50 g The reproducibility and validity of the FFQ was assessed in a random sample of ... For other participants the average of the baseline and follow-up FFQ data were used in the analyses The average daily intake of individual food items (g/day) was combined to compute intake of red ... knowledge there are no other reports of a direct link between poultry consumption and risk of diabetes or of interactions between poultry consumption and BMI and the risk of type DM Red meat consumption...
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Báo cáo y học: "HLA-DR regulation and the influence of GM-CSF on transcription, surface expression and shedding
Ngày tải lên :
03/11/2012, 09:57
... than the level of HLA-DR in the cell lysates (5ul of a in 10 dilution, from x 106 PBMC) The shed HLA-DR therefore seemed to represent a small fraction of the total HLA-DR of the cell, however the ... product and one reverse transcriptase negative product This allowed identification of DNA contamination The sequence of the amplified primer product revealed homology to the primer target sequence The ... expression of both the percentage of monocytes expressing HLA-DR and the density of HLA-DR expression have been observed as a feature of sepsis [4;5;7;8;22-25] Only recently have the possible...
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Báo cáo y học: "Human toxoplasmosis and the role of veterinary clinicians"
Ngày tải lên :
03/11/2012, 11:06
... where the consequences of infection can be very severe, while the risk of living with one’s beloved cat is practically nill References Angulo FJ, Glaser CA, Juranek DD et al Caring for pets of immunocompromised ... with the human immunodeficiency virus Clin Infect Dis.1994;18:14-24 Han K, Shin DW, Lee TY, et al Seroprevalence of Toxoplasma gondii infection and risk factors associated with seropositivity of ... women in Korea J Parasitol 2008; 94: 963-965 Grant S and Olsen CW Preventing zoonotic diseases in immunocompromised persons: the role of physicians and veterinarians Emerg Infect Dis 1999; 5: 159-163...
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UN-REDD programme and the progress of implementing REDD in Vietnam
Ngày tải lên :
23/01/2013, 22:06
... well as the decentralization of power to the Ministry of Agriculture and Rural Development and Ministry of Natural Resources and Environment in the land allocation under Decree 99 The goal of phase ... The participation of the private sector is not strong enough especially the companies working in the field of fisheries, agriculture related to conversion of agricultural land The awareness of ... governments of Vietnam and Norway on the implementation of phase II, Norwegian Government has also agreed to further sponsor Vietnam an amount of US$30 million What is the progress of implementing...
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