CELL SELECTION AND RESELECTION pot
... CELL SELECTION AND RESELECTION TTKTVT HCM NGUYỄN VĂN CƯỜNG VIETTEL CORPORATION CELL SELECTION AND RESELECTION TTKTVT HCM NGUYỄN VĂN CƯỜNG VIETTEL CORPORATION TTKTVT HCM CELL SELECTION AND RESELECTION ... MEASUMENTS FOR CELL SELECTION MS search tất kênh hệ thống (124 for P‑GSM, 174 for E‑GSM, 194 for R-GSM, and 374 for DCS 1 800) MS đọc sóng mang tính toán cường độ trung bình sóng trị trung bình tính toán ... VĂN CƯỜNG VIETTEL CORPORATION TTKTVT HCM CELL SELECTION AND RESELECTION THE END CELL SELECTION AND RESELECTION NGUYỄN VĂN CƯỜNG ...
Ngày tải lên: 11/03/2014, 09:20
... Sciences and Technology 23 (2007) 269-274 explored as potentially more efficient alternatives for the mass production of ginseng and its active components Research into ginseng cell and tissue ... ginseng culture has continued to attract considerable research and development effort in recent years and scientists seek to understand and optimize the culture conditions [2] As earlier reported ... increase of the growth ratio and dry weight were not accompanied by an increase of the saponin content Using MS medium minus NH4NO3 and plus 0.5% glucose and 2% sucrose and 2% sucrose added after...
Ngày tải lên: 12/02/2014, 10:20
... 215–224 Wu J.Y and J.J Zhong 1999 Production of ginseng and its bioactive components in plant cell culture: current technological and applied aspects J Biotech., 68: 89-99 Dornenburg H and D Knorr ... suspension culture of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of ginseng cell culture Materials and methods Stock cell culture and culture condition Stock ... until now were claimed to require 2,4-D which is a potent herbicide and carcinogen and therefore unsuitable for pharmaceutical and food industries [10] From this point of view, our system is apparently...
Ngày tải lên: 14/03/2014, 10:20
Báo cáo khoa học: Super life – how and why ‘cell selection’ leads to the fastest-growing eukaryote doc
... decreases, and the supply rate of Gibbs energy and chemical substrates becomes insufcient for cytoplasm-based catabolic and anabolic processes [43] With regard to identication of what limits the ... (chemostat) and substrate saturation (pH-auxostat) The specic cellular glucose and oxygen consumption rates, together with the specic protein and carbon dioxide production rates (qglu, qO2 , qCO2 and ... dilution rate was and remained 100% Both datasets indicate that no products other than biomass and CO2 were synthesized, conrming that the specic ows of catabolism (qCO2 and qO2 ) and anabolism (lmax)...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo " Effects of macro elements on biomass and ginsenoside production in cell suspension culture of Ngoc Linh ginseng " docx
... conditions were done as described by [9] Results and discussion Effects of macro elements on biomass and ginsenoside production Table and Figure show how growth and yield of ginseng cell were affected ... Higher strengths (1.0, 1.5, and 2.0) of MgSO4 were more favorable for both cell biomass growth and ginsenoside accumulation, as seen by the highest cell biomass DW (10.4 g) and ginsenoside content ... grants from the Department of Science and Technology, Vietnam National University Hanoi (QG.06.14), and Basic Research Program in Life Sciences, Ministry of Science and Technology (6.090.06) to Hanoi...
Ngày tải lên: 28/03/2014, 10:20
Báo cáo khoa học: ERK and cell death: ERK location and T cell selection pdf
... LAT They only activate Ras-GRP1 and induce its recruitment to the Golgi [16] However, the fact that negative selecting ligands activate and recruit both Ras-GRP1 and Grb2 ⁄ SOS to the plasma membrane ... the mechanism of its regulation is still unclear Negative selecting ligands induce a rapid and robust induction of phopho-ERK1 ⁄ 2, whereas positive selecting ligands induce slow and sustained activation ... pathway that ultimately leads to its phosphorylation by ERK5 [70,71] On the other hand, Akt-mediated phosphorylation of Nur77 inhibits its DNA-binding activity [72], and there is speculation that...
Ngày tải lên: 29/03/2014, 08:20
Báo cáo y học: "VLA-4-dependent and -independent pathways in cell contactinduced proinflammatory cytokine production by synovial nurselike cells from rheumatoid arthritis patients" potx
... 0.1% hyaluronidase (Sigma), and 0.01% DNAse (Sigma) The resultant single-cell suspension was plated onto culture dishes and maintained in DMEM (Gibco BLR, Grand Island, NY, USA) containing 10 ... streptomycin, and 10% heat-inactivated FCS (Hyclone Laboratories, Logan, UT, USA) After four to five passages, leukocytes and macrophages were removed from the culture and only the adherent, and apparently ... CD29, CD49e, CD44, and CD54, and only weakly positive for CD106, but were negative for CD11a, CD18, and CD49d (Fig 3) RA-SNC77 cells also expressed a CS-1 isoform of fibronectin and a chemokine...
Ngày tải lên: 09/08/2014, 06:22
Báo cáo y học: "B cells in Sjögren’s syndrome: indications for disturbed selection and differentiation in ectopic lymphoid tissue" doc
... of salivary glands and its relationship to myoepithelial sialadenitis Hum Pathol 1988, 19:766-776 Jaffe ES: Lymphoid lesions of the head and neck: a model of lymphocyte homing and lymphomagenesis ... B-cell-targeted cytokines and survival factors, including BAFF and APRIL (a proliferation-inducing ligand) [50,55] Thus, a tight interplay between activated SGECs, infiltrating lymphocytes and DCs leads ... different types of exocrine gland [66] In patients with pSS and recurrent or persistent swelling of the major salivary glands, the lymphocytic lesions of the involved glands often contain massive...
Ngày tải lên: 09/08/2014, 10:20
Báo cáo y học: "DNA-like class R inhibitory oligonucleotides (INH-ODNs) preferentially block autoantigen-induced B-cell and dendritic cell activation in vitro and autoantibody production in lupus-prone MRL-Faslpr/lpr mice in vivo" potx
... (18, 44, 46, and 48) and palindromic (1, 43, 45, and 47) INH-ODNs lacking the CCT element (45 and 46), GGG element (43 and 44), or both (47 and 48) Similar to control ODN 4173, INH-47 and INH-48 ... to complex TLR ligands [1318] This enhancement may be due to multiple mechanisms (for example, TLR7 and upregulation, increased ligand uptake, BCR-mediated delivery of TLR ligands to 'autophagosomes' ... INH-43, INH-45, and INH-47 are palindromic variants of INH-1 in which the CCT and/ or GGG elements were replaced with random nucleotide triplets Similarly, ODNs INH-44, INH-46, and INH-48 are linear...
Ngày tải lên: 09/08/2014, 14:21
Báo cáo y học: "Type I interferon receptor controls B-cell expression of nucleic acid-sensing Toll-like receptors and autoantibody production in a murine model of lupus" doc
... both transmembrane and soluble isoforms and is critical for ligand binding and signal transduction through the receptor [27,28] Negative regulators of IFN and other proinflammatory cytokine signaling, ... metric and complete linkage method was applied, and results were depicted as a heatmap and dendogram generated using Java Treeview software [36] A full list of antigens included on the array and ... containing 3% FBS and 0.05% Tween-20 followed by isotype-specific HRP-conjugated GAM-Ig (SouthernBiotech) Standard curves were constructed using mouse Ig isotype standards (SouthernBiotech), and total...
Ngày tải lên: 09/08/2014, 14:22
Báo cáo y học: " Transcriptome analysis of functional differentiation between haploid and diploid cells of Emiliania huxleyi, a globally significant photosynthetic calcifying cell" potx
... ribosomal structure and biogenesis Carbohydrate transport and metabolism Energy production and conversion Intracell traffic., secretion and vesicular transport Amino acid transport and metabolism ... Replication, recombination, and repair Coenzyme transport and metabolism Chromatin structure and dynamics Nucleotide transport and metabolism Cell cycle control, division and chromosome partition ... those which are best-hits to Viridiplantae) The differences between 1N-unique and 2N-unique, and between 1N-unique and shared clusters were significant (P < 0.002) Validation and exploration of...
Ngày tải lên: 09/08/2014, 20:20
báo cáo khoa học: " Effect of clone selection, nitrogen supply, leaf damage and mycorrhizal fungi on stilbene and emodin production in knotweed" doc
... treatment (because of its ability to accumulate heavy metals, especially Cd and Pb) and riverbank and sand hill reinforcement [7] However, these qualities also contribute to its competitive advantage ... statistics (F-values and degrees of freedom) and several other plant characteristics, such as stem, branch and leaf numbers, leaf area, SLA, stem and leaf water contents and carbon content, reflecting ... associate with knotweeds and potentionally alter their growth characteristics, their genotype and accumulation of plant secondary compounds [36] Synthesis of resveratrol and its derivatives, especially...
Ngày tải lên: 11/08/2014, 11:20
Báo cáo y học: "Dendritic cell subsets dynamics and cytokine production in SIVmac239-infected Chinese rhesus macaques" pptx
... contributions YTZ and HJX designed this study HJX and GHZ carried out the experiments JPM, ZXD, SYL, and JBH coordinated and performed the primate studies YTZ, HJX, and GHZ analyzed the results and drafted ... day 76 and increased Finally, the number declined and remained at a low level because of the death of 00317 and 98081 (Figure 3A) The mean count of mDC reached the highest site on days 202 and 265, ... 728.3 pg/ml; range, 13 to 3591 pg/ml), and then recovered and remained at a subnormal level until monkeys 98081 and 00079 lost the ability for IFN-a production and died of AIDS (mean, 201 pg/ml;...
Ngày tải lên: 13/08/2014, 01:20
Báo cáo y học: "The global role of ppGpp synthesis in morphological differentiation and antibiotic production in Streptomyces coelicolor A3(2)" pdf
... (0.7 ml) and sterile sand (0.25 g: Sigma -50+70 mesh; Sigma, Gillingham, Dorset, UK), and vortexed vigorously for 90 s to shear genomic DNA and homogenize the sample Cell debris and sand were ... harvested at 0, 30, 60 and 90 minutes Values shown are in pmol mg-1 dry cell weight and are the average of biological triplicate experiments for (a) and duplicates for (b), with standard deviations ... Materials and methods and those referred to in the text are noted in Additional data file reports Illustration of (b) M570 (relA- sampling on cultures of (a) M600 (relA+ Figure and the growth and ppGpp-)...
Ngày tải lên: 14/08/2014, 08:20
Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system
... to pursue PhD and offering me the scholarship And last but not least, this dissertation is dedicated to my family My parents are always standing with me and giving me endless love and encouragement ... for validating and comparing hNESPCs morphology and functional activity in the cells from NP and healthy controls, and demonstrated that the intrinsic factors related to cell growth and differentiation ... and encouragement My husband is always showing his patience and thoughtfulness to my work, and teaching me how to simplify works by using software Without their help and support, it’s hard for...
Ngày tải lên: 09/09/2015, 11:29
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... groups for companionship, support and helpful discussions Most importantly, my family has given me incredible support and encouragement Their love and understanding helped me overcome the obstacles ... an incredible environment to grow and nurture; my sister was amazingly supportive, understanding and a great companion I also thank my fiancé for his patience and companionship, who made long ... co-bound and co-regulated by OCT4, SOX2 and NANOG have been identified (Figure 1.1A) In addition, genes that are co-bound by OCT4, NANOG and SOX2 1 include those that promote pluripotency and self-renewal,...
Ngày tải lên: 09/09/2015, 17:54
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 2
Ngày tải lên: 09/09/2015, 17:54
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... the hippocampus, and its functionally distinct CA1, CA2, CA3, and the dentate gyrus (DG) (e-h) The lncRNAs AK043817, AK159011 and AK017893 were enriched in the cerebral cortex and the labeled ... binding site in its vicinity, and its expression decreased in response to OCT4 RNAi (Figure 6.5A) Pluripotency lncRNAs with a neighboring NANOG binding site (namely lncRNA_ES1 and lncRNA_ES3) ... the nuclear and cytoplasmic fractions, and qPCR was performed to compute nuclear/cytoplasmic ratio HOTAIR and KCNQ1OT1 are well-characterized, nuclear-localized RNAs, while GAPDH and ACTB are...
Ngày tải lên: 09/09/2015, 17:54
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... hnRNPA2B1 and SOX2 as protein partners of the lncRNA Two additional protein bands corresponding to 37 kDa and 35 kDa were observed in the RMST pulldown, and not in the controls (beads only and antisenseRMST, ... steps were performed and proteins were eventually eluted, ran on an SDS-PAGE gel The SDS-PAGE gel was Coomassie-stained, and two protein bands observed in the RMST pulldown and not in the control ... AK056164, AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas...
Ngày tải lên: 09/09/2015, 17:54