are they possible vehicles for gene therapy

... additional genetic events are required The majority of the cancers caused by these retroviruses are found in the haematopoietic system although sarcomas are also common They are also able to transform ... retroviral genes Oncogene capture The mechanism of oncogene capture appears to be dependent on the generation of a chimeric retroviraloncogene transcript (69, 70) This suggests that the risk of oncogene ... and insertional mutagenesis [71,72] are the two risk factors of significance in retroviral mediated gene therapy The two known instances where insertional mutagenesis/ oncogene activation has...

Ngày tải lên: 14/08/2014, 19:22

... vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 11 Kabanov, A V and Kabanov, V A (1995) DNA complexes with polycations for the ... molecular weight is possible The mobile phases we used are 0.1 M NaNO3 for HA fragment analysis and 0.2 M Na2SO4 containing mM sodium phosphate buffer (pH 8.0) for copolymer analysis For typical analysis, ... luciferase assays are performed as described in the protocol of PicaGene luminescence kit using a luminometer The protein concentrations of the cell lysates are measured by Bradford assay using...

Ngày tải lên: 11/04/2014, 09:59

... vectors for gene transfer and gene therapy I would like to thank the many chapter authors for their contributions They are all experts in various aspects of viral vectors, and I appreciate their efforts ... complement the deleted IE genes from the defective virus The cells are cotransfected with viral genomic DNA Plaques form as viral genes are expressed and virions are generated The recombination ... Krisky, D., Oligino, T., et al (2000) Genetically modified CD34+ cells as cellular vehicles for gene delivery into areas of angiogenesis in a rhesus model Gene Ther 7, 43–52 140 Laquerre, S., Anderson,...

Ngày tải lên: 11/04/2014, 10:39

... of a foreign gene, EGFP under the transcriptional control of the HCMV major immediate early (MIE) promoter was used Page of 11 (page number not for citation purposes) Genetic Vaccines and Therapy ... demonstrates that a foreign gene can be expressed in the context of a HCMV amplicon viral stock in infected HF cells To test the utility of the HCMV amplicon in gene therapy or gene delivery, we ... context of a lentiviral-based gene transfer system this promoter appeared to function less Page of 11 (page number not for citation purposes) Genetic Vaccines and Therapy 2005, 3:1 http://www.gvt-journal.com/content/3/1/1...

Ngày tải lên: 14/08/2014, 19:22

... gene therapy 14 2.1.2 Hurdles for gene therapy 15 2.1.3 Systems for gene delivery 16 2.2 Viral vectors for gene delivery…………………………………………… 17 2.3 Non-viral vectors for ... for gene therapy because they could achieve sustained and highly efficient transfection level in gene delivery The number of different viruses that are under development as vectors for gene therapy ... our lab to the gene delivery system 12 Chapter Literature Review 2.1 Gene therapy 2.1.1 Overview of gene therapy Gene therapy is a rapidly advancing field with great potential for the treatment...

Ngày tải lên: 05/10/2015, 21:33

... and thus the former is the vector of choice for future gene therapy trials The number of gene therapy trials in RA is in sharp contrast to the more than 600 clinical trials of gene therapy in patients ... safety of local gene therapy Gene therapy in RA is still in its adolescence, and more clinical studies must be initiated if it is to mature It is, however, imperative that gene therapy studies ... Sacchi A: Wild-type p53 gene transfer is not detrimental to normal cells in vivo: implications for tumor gene therapy Oncogene 2004, 23:418-425 Kruyt FA, Curiel DT: Toward a new generation of conditionally...

Ngày tải lên: 09/08/2014, 01:24

... source for MSCs and NSCs in suicide gene therapy 113 5.1.1 Baculoviruses as gene therapy vectors for human cancer 113 5.1.2 HESC- derived MSCs and NSCs as gene delivery vehicles for glioma therapy ... 1.1 Percentage of each gene therapy strategy for clinical trials (Michael Edelstein, 2012) 1.1.2 Gene carriers for cancer therapy Successful gene therapy relies largely on gene delivery efficiency ... for cancer gene therapy 24 1.2.3 New reliable sources for therapeutic stem cells 26 1.3 Current mouse models in cancer research 28 1.3.1 Syngeneic models 28 1.3.2 Xenogeneic models 29 1.3.3 Genetically...

Ngày tải lên: 09/09/2015, 10:08

... vectors in gene therapy for glioma Finally, this study demonstrated, for the first time, that human embryonic stem cells can provide a potentially unlimited source for glioma gene therapy Using ... of low gene transduction in patients Viral, chemical, and cellular vectors are being studied as vehicles for gene delivery 1.2.1 Viral vectors Viral vectors are the most effective in vivo gene ... cell vectors in glioma gene 20 therapy 1.3.6 Stem cell based glioma gene therapy 1.3.6.1 Therapeutic genes 22 22 1.3.6.1.1 Prodrug-converting enzymes 22 1.3.6.1.2 Other gene payloads 25 1.3.6.2...

Ngày tải lên: 11/09/2015, 09:00

... for BRCA1/2 mutation carriers Why are BRCA1- and BRCA2-deficient cells extremely sensitive to PARP-1 inhibition? The products of the genes, BRCA1, BRCA2 and PARP-1, are nuclear proteins and are ... PARP-1-null mice are viable and healthy [32], and inhibitors that are specific for PARP-1 are relatively non-toxic and not directly damage DNA, PARP-1 inhibitors might be both safe and effective for BRCA1/2-associated ... during spermatogenesis in BRCA1 full-length isoform deficient mice Development 2003; 130: 2001-12 Brodie SG, Xu X, Qiao W, et al Multiple genetic changes are associated with mammary tumorigenesis in...

Ngày tải lên: 31/10/2012, 16:57

... otherwise standard MuLV vectors for gene therapy has not been successful Beyond these HEMATOPOIETIC STEM AND PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 137 efforts, there are obviously major safety ... PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 143 144 GENE THERAPY FOR HEMATOLOGICAL DISORDERS restoration of cellular and humoral immunity were documented after gene therapy Data showed a surprisingly ... transfer techniques are advantageous for gene therapy approaches when applied to hematological diseases Aspects of ex vivo gene transfer as well as certain gene transfer vector systems are particularly...

Ngày tải lên: 10/04/2014, 22:10

... it is necessary for any vehicle used for in 156 GENE THERAPY FOR LIVER DISEASE vivo hepatic gene therapy to reach the liver efficiently For systemic application, the gene vectors are ideally targeted ... application for hepatic gene delivery, while the second part will address the clinical applications attempted so far GENERAL PRINCIPLES FOR HEPATIC GENE THERAPY There are two basic approaches for gene ... virus (HDV) are known to cause persistent infection and chronic disease of the liver and are serious threats to public health Gene therapy approaches to therapy are the 180 GENE THERAPY FOR LIVER...

Ngày tải lên: 10/04/2014, 22:11

... competes for an essential substrate or co-factor that is available in limiting amounts, or, for proteins that form multimeric complexes, the 270 GENE THERAPY FOR HIV INFECTION TABLE 11.1 Gene Therapy ... gene expression Thus, the antisense gene expression must be quantatively higher than the levels of HIV-1 gene expression for an antisense gene therapy strategy to be effective Standard gene therapy ... MKL, Morgan RA Gene therapy for acquired immune deficiency syndrome AIDS Updates 7:1–14, 1994 Yu M, Poeschla E, Wong-Staal F Progress towards gene therapy for HIV infection Gene Therapy 1:13–26,...

Ngày tải lên: 10/04/2014, 22:11

... assays were performed using a LightCycler (Roche Diagnostics) with specific primers for WT1 and the housekeeping gene porphobilinogen deaminase (PBGD) as described elsewhere [22] For quantification, ... could represent a possible immune escape mechanism as they may abolish proper HLA class I binding, T cell recognition or proteasomal processing Therefore, mutation analysis was performed in both ... stained positive for HLA-A2 In none of the patients a difference of HLA-A2 expression before therapy and at the time point of progression was observed Conclusions We have no evidence for an immune...

Ngày tải lên: 18/06/2014, 16:20

... important for the resistance to possible introduction of genes using viral vectors or for the resistance to oncolytic gene therapy We believe that this finding can be of crucial interest for the ... viral gene therapy approaches Adenoviruses and Adeno-Associated viruses are used to deliver genes to tumor cells with the goal of modifying the phenotype, as for example, by introducing suicide genes ... Baker SW, Khodarev N, Su AW, Shaikh AY, Roach P, Kreike B, et al: An interferon-related gene signature for DNA damage resistance is a predictive marker for chemotherapy and radiation for breast...

Ngày tải lên: 18/06/2014, 16:20

... as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading ... (FA), heparin and so on [4] Furthermore, several PEI-based delivery vehicles have been used to carry DNA for gene therapy [9,14,15] For example, a liner PEI-cholesterol conjugation was encapsulated ... electrostatic interaction to form nanocomplexes, are widely attempted to use as gene delivery systems The advantages associated with this kind of vectors include that they can protect DNA from nuclease...

Ngày tải lên: 18/06/2014, 19:20

... as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading ... (FA), heparin and so on [4] Furthermore, several PEI-based delivery vehicles have been used to carry DNA for gene therapy [9,14,15] For example, a liner PEI-cholesterol conjugation was encapsulated ... electrostatic interaction to form nanocomplexes, are widely attempted to use as gene delivery systems The advantages associated with this kind of vectors include that they can protect DNA from nuclease...

Ngày tải lên: 20/06/2014, 03:20

... to be answered for successful CF gene therapy have now been clearly defined, and gene therapy for CF remains the most promising possibility for curative rather than symptomatic therapy 12 13 14 ... models that successful gene therapy for cystic fibrosis requires only partial gene correction Gene Ther 1996, 3:797–801 28 Hyde SC, Southern KW, Gileadi U, Fitzjohn EM, Mofford KA, Waddell BE, ... are encouraging Respiratory Research Vol No Stern et al The inevitable pessimism that follows unrealistic expectations of a rapid cure has dogged the recent media view of gene therapy Gene therapy...

Ngày tải lên: 12/08/2014, 18:20

... Churchill are relevant: “We are not at the end, nor at the beginning, we are not at the beginning of the end but perhaps we are at the end of the beginning” Competing interests None declared References ... will be reforming their health care services in an attempt to limit costs escalating ICUs will not be immune from this, and it is therefore in our own interests to take responsibility for using ... Available online http://ccforum.com/content/7/6/420 a nurse’s or doctor’s time were spent in duties other than direct patient care, then this must have a deleterious effect on the care of the patient,...

Ngày tải lên: 12/08/2014, 20:20

... blood [1,19] Thus, CD34 progenitor cells are an essential ingredient for HIV gene therapy In view of the need for CD34 cells for HIV gene therapy as well as for other hematopoietic disorders, if ... lineages thus paving the way for utilization of these cells for hematopoietic cell therapy [20,27-29] For the effective utilization of hES-CD34 cells for HIV gene therapy, a number of parameters ... drug based therapies for a complete cure, new and innovative approaches are essential Gene therapy through intracellular immunization offers a promising alternative approach and possible supplement...

Ngày tải lên: 13/08/2014, 09:21

... I IFNs for the treatment of HIV infection could be increased by developing a gene therapy strategy based on the modified production of IFN-β in genetically engineered lymphocytes [18] For this ... macaques maintained high CD4+ T-lymphocyte counts for at least 478 days [24] However, a gene therapy strategy for HIV infection would only be possible during the chronic phase of infection At ... study, performed with animals infected for more than one year, cells transduced with the IFN-β construct rapidly disappeared from the bloodstream after infusion This suggests that gene therapy...

Ngày tải lên: 13/08/2014, 13:20