... one-turn helix on the other face It is thought that the two inner strands ofthe b-sheet lie within the major groove ofthe DNA and that a hydrophobic pocket formed by the side chains of Y123 and I125 ... [18] The structure ofthe helical coil a2/a3 allows us to interpret the consequences ofthe six mutations As P153(152) and G162(161) are buried in the protein core, replacement of each of these ... cluster in these two regions Knowledge ofthe structure of a larger portion of MeCP2 including the transcriptional repression domain would resolve these speculations and clarify the role ofthe helical...
... mobility with respect to the rest ofthe structure Further studies ofthe receptor bound forms ofthe chimera and other related ligands will be necessary to define the nature ofthe interactions leading ... Several of these were identified in previous studies of EGF, and the backbone fold ofthe chimera is clearly similar to those ofthe EGF structures The presence of a backbone hydrogen bond from the ... interrupted by the presence of two proline residues, in which cases the following NOEs were observed: Ha/Hb ofthe previous residue to the Pro H* and NH ofthe following residue to the Pro Ha/Hb The assignments...
... (B) Representation of flexibility in thesolution structure ofthe RICH catalytic domain The width ofthe sausage is reversely proportional to the heteronuclear NOE values The figure was generated ... FEBS 1601 Solution structure ofthe RICH catalytic domain G Kozlov et al Results Structure ofthe RICH catalytic domain We determined the structure ofthe 24 kDa catalytic fragment of goldfish ... helical fragments group together in the vicinity ofthe N-terminus This helical patch is the most basic part ofthe molecule and a potential interaction surface for the preceding acidic N-terminal...
... establish the preferred geometry ofthe precursor We now report the chemical synthesis, biochemical production, and solution structure of preCbnB2, and compare it with the structure ofthe mature ... help determine the structural basis ofthe inhibition ofthe antimicrobial activity of CbnB2 by the leader and to assist future analysis ofthe interaction of preCbnB2 with its ABC transporter ... for cleavage ofthe leader peptide after the double glycine motif [16,17] This cleavage occurs on the cytoplasmic side ofthe membrane during export ofthe bioactive peptide [18] The leader peptide...
... ensemble of HPr(I14A) The average structure ofthe 10 lowest-energy structures out of 300 calculated with CNS is shown The radius ofthe spline reflects the RMSD values ofthe Ca atom positions The ... atoms of Ala19 In addition, the C-terminus folds back onto the core ofthe protein thereby allowing the side chain of Leu86 to partly fill the hole created by the removal of Ile14 Due to these ... computed as the ratio ofthe standard deviations ofthe chemical shifts ofthe amide nitrogen and proton nuclei Results Determination ofthe three-dimensional structure of HPr(I14A) To allow the comparison...
... either the LPS core structure or/and in the mode ofthe attachment ofthe OPS to the core Therefore, strains of pathovar porri are clearly distinct from other P syringae pathovars in serology of ... and the similarity ofthe C2-C6 chemical shifts ofthe GlcNAc residue to those of nonsubstituted b-GlcNAc [32] These data showed that the major O repeat ofthe OPS has structure 2: each other ... (Table 1) The OPS of chemotypes 3C, 8C and 9C differ from each other in the site ofthe attachment of b-D-GlcNAc residues to the main L-rhamnan chain (Table 4) The OPS of P syringae pv atrofaciens...
... some light on the steps involved in the fibrillogenesis, but, most of all, to evaluate the role of Ab-(1–42) in the interaction with the membrane The structure of Ab-(1–42) found in aqueous hexafluoroisopropanol, ... Furthermore, the Ab-(1–42) solution in aqueous HFIP was very stable, as there was no evidence of aggregation or precipitation and the NMR spectra did not change over several weeks The quality of ... to those ofthe lipid phase ofthe membrane, which promotes the formation of short-range H-bonds inducing helical structures The structural characterization of a monomeric, soluble form of Ab-(1–42)...
... elbowÕ of C5 is located at the upper left corner ofthe figure when C5 is in the presence ofthe cosolvents trifluoroethanol or hexafluoroisopropanol The high-resolution solution structure of HIV ... nonhydrogen) ofthe ensemble to the ˚ mean is 1.25 A In Fig 4B, the backbone RMSD ofthe C5 ensemble is plotted as a function of residue number to illustrate the relative uncertainties ofthe backbone ... map ofthe minimized mean structure of HIV gp120 C5 (A) The location ofthe furin/PC7 site is shown The locations ofthe missing gp120 domains and gp41 are denoted (B) In this orientation, the...
... mm of monomers at 30 °C Most ofthe 1H-15N HSQC signals ofthe dimeric form of PDI-bb¢ coincide with the signals ofthe monomeric form or are weak as a result ofthe high molecular weight ofthe ... structure, the catalytic cysteines face each other; in the other, the catalytic residues ofthe a domain face away from the a¢ domain The crystal structures also revealed the presence of a hydrophobic ... Superimposition ofthesolution structure ofthe human PDI-b¢ (blue) with the crystal structure of yeast PDI-b¢ (red, Protein Data Bank entry code 2B5E) (C) Superimposition ofthesolution structure of human...
... helices and of P16 (Fig 1) Helices 5002 Fig Stereo views ofthesolution and crystal structures of DnaG-C (A) Superposition ofthe backbone atoms of residues 447–581 ofthe 20 NMR conformers of DnaG-C ... resolve these difficulties and the discrepancies between the structure of P16 (which is monomeric in solution) and the different conformers in the crystal structure of DnaG-C, we here report thesolution ... stearothermophilus DnaB confirmed the importance of residues in these parts ofthe protein [3,16] Unlike in the wildtype protein, the individual N-terminal and C-terminal domains of B stearothermophilus...
... of GalA GLC analyses ofthe acetylated (S)-2-(+)-butyl glycosides demonstrated the d configuration of Glc, GlcN, GalN, and GalA The d configuration of Qui3N was established by the analysis ofthe ... Proteus [16] Studies ofthe O -polysaccharides of Proteus genomospecies and and the comparison ofthe corresponding 13C NMR spectra indicated that they are identical to those of P vulgaris O8 [17] ... Proteus genomospecies Fig Part of a 2D ROESY spectrum ofthe polysaccharide of Proteus genomospecies The corresponding parts ofthe 1H-NMR spectrum are displayed along the axes Arabic numerals refer...
... though they use the Internet at libraries 22% of adults 65 and older have access to the Internet Ofthe 22% ofthe US adults aged 65 and over using the Internet, it is estimated that 66% use the ... was one ofthe most popular past-times ofthe elderly interviewed in his study The majority of them used the library to rent books, audio, videos and participate in library programs If the elderly ... time they have with their doctor count Complicating the results of other studies that indicate the elderly prefer communication with their doctors to gather medical information, the Gladden study...
... with the large size ofthe cavity easily allowing substrate entry However, the side chains of residues Tyr988 and Lys987 ofthe second molecule ofthe ‘dimer’ clash with the region where the adenosyl ... that either: (1) the concentration ofthe dimer species under crosslinking conditions is very small; (2) the location ofthe N-terminus in solution is not consistent with models ofthe dimer (the ... represents the change in fluorescence intensity, F0 is the measure ofthe fluorescence intensity in the absence ofthe activation domain, E0 is the concentration ofthe FMN domain, L denotes the concentration...
... understanding ofthe characteristics of this peptidase, which would be of industrial use in the debittering of fermented foods Results and Discussion Cloning and expression of Lc lactis prolidase The prolidase ... activity was seen in the presence of manganese Moreover, the preference for dipeptide changed from Leu-Pro to Arg-Pro in the presence of manganese A comparison ofthe crystal structure of P furiosus ... is the length ofthe loop structure that is contributed by the other subunit and covers the S1 site (yellow ribbon for P furiosus and cyan ribbon for Lc lactis in Fig 5) The crystal structure of...
... investigated thebehaviorofthe solutions, HAE-M found the solutions ofthe special cases and EMEls carried out the theoretical proof and gave the examples All authors read and approved the final ... investigated thebehaviorofthesolutionof difference equations for example: Elabbasy et al [5] investigated the global stability, periodicity character and gave thesolutionof special case ofthe ... and rewarding, and the results about these equations offer prototypes towards the development ofthe basic theory ofthe global behaviorof nonlinear difference equations of a big order, recently,...
... , the bifurcation structure ofthe nodal solutions of 1.8 , 1.9 becomes more complicated: the component ofthe solutions of 1.8 , 1.9 from the trivial solution at λk /f0 , and the component of ... 1.9 , and use the global bifurcation theorems from Section to analyze the global behaviorofthe components of nodal solutions of 1.8 , 1.9 Preliminary definitions and eigenvalues of corresponding ... Let (H0) and 3.6 hold If μ, u ∈ E is a nontrivial solutionof 3.3 , then u ∈ Tk for some k, ν Proof The proof of Lemma 3.6 is similar to the proof of Lemma 3.1 omit it 4, Proposition 4.1 ; we ν...
... completes the proof ofthe proposition Behaviorofthe positive solutions of fuzzy equation (1.1) In this section, we study thebehaviorofthe positive solutions of (1.1) Firstly, we study the periodicity ... order to study thebehaviorof a parametric fuzzy difference equation we use the following technique: we investigate thebehaviorofthe solutions of a related family of systems of two parametric ... Existence and uniqueness ofthe positive solutions of fuzzy difference equations (1.1) and (1.2) In this section, we study the existence and the uniqueness ofthe positive solutions ofthe fuzzy difference...
... bounded, then the height distribution Derivation of Results We establish the five parts of Theorem Since the analysis involves a routine use ofthe saddle point method (cf [1, 12]), we only give the ... for large b (cf Theorem 2) The proof is delayed until Section It is based on an asymptotic evaluation of a certain integral Summary of Results We let Hn be the height of a b-trie of size n We denote ... better understood by viewing the problem as first fixing k and b, and then varying n (cf Section 4) Theorem For b → ∞ the distribution ofthe height of b-tries has the following asymptotic expansions:...
... then yields the result 3.2 (λ([0, x])(1 − λ([0, x])))p/2 The proof of Theorem Proof The proof is completely analogous to the proof of Theorem 1, due to the fact that the entire structure ofthe ... |g(a) − g(b)| dadb The result then follows from Theorem and ess sup0 3.5 x ∗ |g(x)| = DN (P) The proof of Theorem P Proof We go back to our definition of DN as the expected value ofthe random variable ... suitable decompositions ofthe unit cube (i.e bounds on the volume ofthe set A from the proof) The second way seems to lead to a very technical path while the first way seems to be the more manageable...
... investigate thebehaviorofthe Lannes-Zarati homomorphism in the ranks five and six The vanishing ofthe fifth Lannes-Zarati homomorphism is given in the following theorem Theorem 5.1 The homomorphism ... other words, the diagram Exts,t A (F2 , F2 ) Sq ϕs P Rs / Exts,2t (F2 , F2 ) A ϕs Sq / P Rs commutes The detail description ofthe action of Sq i s on the dual ofthe Dickson algebra and the ... Then the Dyer-Lashof algebra is defined as the quotient of lambda algebra by the two-sided ideal generated by all monomials of negative excess / R A Denote by Qi is the image of λi through the...