aptamer targeted rnai for hiv 1 therapy

... HIV- 1 envelope protein-expressing cells [see comments] Cell 19 97, 90:8 41- 847 Page 13 of 15 (page number not for citation purposes) Retrovirology 2005, 2:80 10 11 12 13 14 15 16 17 18 19 20 21 ... Particleb Env+ Env- Env+ Env- Env+ Env- HIV- 1- Neo(w/o CD4) HIV- 1- Neo(CD4) HIV- 1- Neo(CD4/gpi) HIV- 1- Neo(G) 403 363 5 91 517 446 3 550 4 01 3 91 4 a105 Env+ TF228 or 10 5 Env- BJAB cells were infected and ... http://www.retrovirology.com/content/2 /1/ 80 A 749 - 787 6307 - 7755 86 51 - 9709 HDPack1 polyA HIV- 1 rev vpr pol gag env RRE vif tat vpu LTR tat rev LTR nef 64 01 - 7 613 Nde1 Bgl2 HIV- 1- Neo SV40 Promoter neomycinr 14 0 D 12 0 10 0...

Ngày tải lên: 13/08/2014, 09:21

... 0.089 E138K-PETT-2 SRS PX14.2 CCD ADSC-Q4 0.979 13 8.8, 11 4.8, 65.9 30.0–3.0 575 71 19095 88.0 11 .8 0.083 2.59–2.50 33 71 92.5 1. 0 2.59–2.50 3627 99.5 1. 1 3 .11 –3.00 15 37 72.6 1. 4 30.0–2.5 33264 ⁄ 17 40 ... K101E-nevirapine PF BL-6 A Fuji BAS III 1. 000 13 8.9, 11 4.9, 65.6 30.0–2.5 14 98 21 35057 94.4 9.8 0 .10 0 E138K-nevirapine APS SBC-2 CCD SBC 0.82657 13 9.6, 11 5.0, 65.6 30.0–2.5 2 516 96 3 710 4 99.5 12 .6 ... the HIV- 1 RT subunits as indicated (A) Lys101Glu in p66 and nevirapine (B) Glu138Lys in p 51 and nevirapine (C) Glu138Lys in p 51 and PETT-2 Fig 1, i.e for Lys101Glu in the p66 subunit (Fig 1A)...

Ngày tải lên: 16/03/2014, 13:20

... Virology Journal 2009, 6 :18 4 http://www.virologyj.com/content/6 /1/ 184 A Combination marker M M CM M 2.0 kb 1. 5 1. 0 0.75 0.5 Population # 11 12 13 14 10 16 17 18 19 15 M 21 22 23 24 Not detected ... Proc Natl Acad Sci USA 2004, 10 1:4 912 -4 917 Page 14 of 15 (page number not for citation purposes) Virology Journal 2009, 6 :18 4 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 Lee MT, ... profiles for each scenario modeled Scenario 6× 10 0 90 50 0 % of cells with combinations of the indicated shRNA number per scenario 5× 4× 3× 2× 0 0 10 0 0 10 0 2 2 10 10 10 10 90 50 20 15 1 0 10 15 The...

Ngày tải lên: 12/08/2014, 04:20

... Biol Interact 19 94, 91: 111 -12 1 Anderson ME: Glutathione: an overview of biosynthesis and modulation Chem Biol Interact Chem Biol Interact 19 98 Apr 24 ;11 111 2 :1- 14 19 98, 11 1 -11 2 :1- 14 Zhao M, Rudek ... type promoter J Virol 2007, 81: 10 914 -10 923 Page of 10 (page number not for citation purposes) Retrovirology 2009, 6:52 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Khan N, Jeffers ... here for file [http://www.biomedcentral.com/content/supplementary /17 424690-6-52-S2.ppt] 10 11 12 13 14 Hamer DH: Can HIV be Cured? Mechanisms of HIV persistence and strategies to combat it Curr HIV...

Ngày tải lên: 12/08/2014, 23:21

... milestone for our understanding of host cell – HIV- 1 interaction References Goff SP: Knockdown screens to knockout HIV- 1 Cell 2008, 13 5: 417 -420 10 11 12 13 14 15 16 Cherry S: What have RNAi screens ... http://www.retrovirology.com/content/6 /1/ 78 Table 1: Selected HIV- 1 cofactors identified in the siRNA and shRNA screens Gene name Protein function Possible role in HIV- 1 life cycle MED4 MED7 MED14 MED28 NUP98 NUP155 NUP 210 GCC1 ... different HIV- 1 cofactors in the screens [1] Figure for HIV- 1 replication cofactors A comparison between siRNA- and shRNA-based screens A comparison between siRNA- and shRNA-based screens for HIV- 1 replication...

Ngày tải lên: 12/08/2014, 23:22

... Temporal gene regulation during HIV- 1 infection of human CD4+ T cells Genome Res 20 01, 11 :11 98 -12 04 42 Imbeault M, Ouellet M, Tremblay MJ: Microarray study reveals that HIV- 1 induces rapid type-I interferon-dependent ... detected with a 1: 2000 dilution of rabbit antiGFP AB (Abcam) Viral envelope, GAG and Nef was visualized by 1: 5000 dilutions of human-anti -HIV- 1 gp120 Ab2G12, 1: 5000 diluted rabbit-anti -HIV- 1 p24 (provided ... ubiquitin-conjugating pathway J Virol 19 98, 72:2280-2288 doi :10 .11 86 /17 42-4690-7 -1 Cite this article as: Schindler et al.: Vpu serine 52 dependent counteraction of tetherin is required for HIV- 1 replication in macrophages,...

Ngày tải lên: 12/08/2014, 23:22

... RRE +RU5PS MLV /HIV RU5PS MLV 10 0 10 0 0.20% 75 -Rev 50 10 0 10 2 10 3 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4 GFP Neg 1. 04% 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4 0.30% 10 2 10 3 10 4 10 0 10 1 10 2 75 10 3 10 4 25.92% GFP ... 500 10 0 25 10 00 750 10 0 250 10 1 10 2 10 3 10 4 10 0 GFP Neg 50 +RT Inhibitor 10 1 75 10 1 10 2 10 3 10 4 -Rev 10 2 10 3 10 4 MLV /HIV RRE + RU5PS, P4 0.04% GFP Neg 50 25 10 00 10 00 12 .03% 750 MLV 500 10 0 10 2 ... 750 MLV 500 10 0 10 2 GFP 10 3 10 4 10 0 10 2 10 3 0.39% 75 250 10 1 10 1 10 4 10 0 GFP Neg 500 250 10 0 12 .70% 750 GFP Neg +Rev GFP Neg 50 25 10 1 10 2 10 3 10 4 GFP 10 0 10 1 10 2 10 3 10 4 GFP Figure Transduction...

Ngày tải lên: 13/08/2014, 01:21

... (unspliced) 10 2 10 1 10 4 10 3 1. 8 Kb (spliced) RFU 10 2 10 1 10 4 kb 1: 2.9 10 3 GAPDH 10 2 1: 2.3 kb 10 1 12 16 20 24 28 12 cycles 1. 8 kb 16 20 24 28 12 X 16 pCMV-HA 20 24 28 HA-Matrin 1: 1.2 Figure Matrin stabilizes ... NCOA6 Raver1 Raver1 NP_597709 Matrin MATR3 NP_0 011 818 83 RNA binding motif protein 10 RBM10 NP_005667 fibrosin -1- like protein KIAA1545; FBRSL1 NP_0 011 3 611 3 protein BAT2-like KIAA0 515 ; BAT2L1 NP_037450 ... 2 011 , 8: 61 http://www.retrovirology.com/content/8 /1/ 61 Page of 10 B A GAG/POL, genome ~9 TAT/REV/NEF ~4 total kb nuclear cytoplasmic kb 10 4 10 3 ~ 1. 8 ENV/VIF/VPR/VPU kb Kb (unspliced) 10 2 10 1 10 4...

Ngày tải lên: 13/08/2014, 01:21

... ing er Cell lysate Binding to Vif Wt + MDM2 D 320 C + 15 4 320 + CN1 15 4 + N1 3 21 - N2 16 7 412 16 8- 411 311 228 292 345 - Zn 16 8 16 8- 411 - AD 411 + Figure MDM2 bound Vif in its central domain MDM2 ... the Page of 12 (page number not for citation purposes) Retrovirology 2009, 6 :1 http://www.retrovirology.com/content/6 /1/ 1 MDM2 IP : anti-Vif 23 -7 4-4 23 -43 23 -74 75 -11 11 0 -1 4-4 41 Mo ck Wt ... MDM2 200 E 10 00 Control siRNA p24 (pg/ml) 11 12 RF + - D 0% C p55 Cell lysate 11 14 18 21 Post infection (days) Cell Lysate -actin MDM2 APOBEC3G Vif Figure MDM2 negatively regulated HIV- 1 replication...

Ngày tải lên: 13/08/2014, 05:21

... Inhibiting hiv- 1 entry with fusion inhibitors Curr Med Chem 2003, 10 :16 33 -16 42 Page 21 of 23 (page number not for citation purposes) Retrovirology 2005, 2:20 10 11 12 13 14 15 16 17 18 19 Kashanchi ... HIV- 1 infected cells Probe Set ID Accession # 34083_at 35323_at 318 58_at 3 216 5_at 32556_at 33372_at 39628_at 2029_at 35255_at 11 91_ s_at 11 92_at 37350_at 11 04_s_at 36 614 _at 35467_g_at AA 311 1 81 ... 37350_at 11 04_s_at 36 614 _at 35467_g_at AA 311 1 81 U78525 X07 315 L 418 87 X64044 AI189226 AI6 715 47 N36267 AF098799 AB00 310 2 AB00 310 3 AL0 311 77 M 117 17 X87949 W73046 Gene Description splicing factor, arginine/serine-rich...

Ngày tải lên: 13/08/2014, 09:21

... therapeutic targets William Pardridge (University of California, Los Angeles, USA) used intravenous injection of RNAi as an astoundingly successful therapy for brain cancer in mice The epidermal growth ... contends that, unlike Iressa, RNAi- based gene therapies can be designed for both normal EGFR and the mutant forms of EGFR found in many tumors Continuing the theme of RNAi- based therapies, Beverly ... approach to therapy for neurodegenerative disease Spinocerebellar ataxia type (SCA1) results from the expansion of a trinucleotide repeat (CAG) in the coding region of the ataxin -1 gene, resulting...

Ngày tải lên: 14/08/2014, 14:21

... tested for resistance mutations at Week postpartum, 11 (15 .1% ) Page of had detectable 3TC resistance mutations Among 11 1 of the 13 3 sdNVP-exposed women tested at Week postpartum, 19 (17 .1% ) had ... nevirapine to prevent HIV- 1 vertical transmission (HIVNET 012 ) AIDS 20 01, 15 (15 ) :19 51- 1957 Pujades-Rodriguez M, O’Brien D, Humblet P, Calmy A: Second-line antiretroviral therapy in resource-limited ... Perinatal HIV Prevention Trial Group: Intrapartum exposure to Ekouevi et al Journal of the International AIDS Society 2 010 , 13 :28 http://www.jiasociety.org/content /13 /1/ 28 10 11 12 13 Page of...

Ngày tải lên: 20/06/2014, 08:20

... 40 327 ( 41. 4%) 277 (35 .1% ) 1. 10(0.68, 1. 78) 1. 09 (0.67, 1. 80) HIV- 1 647 (82.0%) 1. 0 HIV- 2 10 3 (13 .1% ) 0.73 (0.42, 1. 26) 39 (4.9%) 1. 07 (0.49, 2. 31) 0.484 CD4 count: (n = 787)

Ngày tải lên: 10/08/2014, 05:22

... Gen Virol 2 010 , 91( Pt 1) :1- 12 Pollard V, Malim M: The HIV- 1 Rev Protein Ann Rev Microbiol 19 98, 52:4 91- 532 Hope TJ: The ins and outs of HIV Rev Arch Biochem Biophys 19 99, 365 :18 6 -19 1 Madsen JM, ... and enhancer-bound SR proteins Mol Cell 20 01, 8(6) :13 51- 13 61 15 Graveley BR: Sorting out the complexity of SR protein functions RNA 2000, 6(9) :11 97 -12 11 16 Hallay H, Locker N, Ayadi L, Ropers D, ... Received: 13 April 2 011 Accepted: 17 June 2 011 Published: 17 June 2 011 References Stoltzfus CM, Madsen JM: Role of viral splicing elements and cellular RNA binding proteins in regulation of HIV- 1 alternative...

Ngày tải lên: 13/08/2014, 01:20

... cCGTCTTCGTc 15 25 RGQELRR; QQTNLTR; QGNTLTR 15 18 -15 28 57 15 18 aGAAGCAGAAt 15 28 15 18 tCTTCGTCTTa 15 28 QGSNLAR; QSTTLKR; QRNNLGR 315 6- 316 6 11 3 315 6 cGGAGAGGCTa 316 6 315 6 gCCTCTCCGAt 316 6 NKQALDR; ... a-Helix (F1;F2;F3) 21- 32 31 tGCAGAGTGTc 21 31 aCGTCTCACAg 21 RHQHLKL; RQDNLGR; QSNVLSR 50-60 50 aGAAGACAGGg 60 50 tCTTCTGTCCc 60 RRAHLLN; DRGNLTR; QSNNLNR 15 15 -15 25 56 15 15 gGCAGAAGCAg 15 25 15 15 cCGTCTTCGTc ... cure for HIV: the identification and characterization of HIV reservoirs in optimally treated people Cell Res 2 011 , 20 (11 ) :11 85 -11 87 The Council of the Global HIV Vaccine Enterprise: The 2 010 scientific...

Ngày tải lên: 13/08/2014, 16:20

... this codon using equation (18 ) is given by  21 = 2.5 × 10 -5 Then, using equation (17 ), we have  31 = f3  1. 4 × 10 -14 and 32 = f2  4 .1 × 10 -10 13 14 15 16 17 18 19 20 Acknowledgements This ... http://www.tbiomed.com/content/5 /1/ 25 Probability that a mutant has occurred 0.8 14 10 14 10 ε 31= 1.4× 10 ε 31= 1.4× 10 , ε32=4 .1 10 , ε32=4 .1 10 , Equilibrium , Transient 0.6 0.4 0.2 0 10 20 30 40 50 Weeks ... by setting zero T = P1 = P2 = † 21 and other higher-order terms to P + O(† 21) fk1 ⎛ T †21k ⎞ (R′ 01 − 1) ⎜ − ⎟ + O(† 21) k1 f (k1 − k ) ⎠ ⎝ † 21 T (R′ 01 − 1) + O(† 21) , f (k1 − k ) where the wild-type...

Ngày tải lên: 13/08/2014, 16:21

... receptor and inhibition of steroidogenesis by an Ó FEBS 2002 15 16 17 18 19 20 21 22 HIV TAT-CRAC peptide Proc Natl Acad Sci USA 98, 12 67± 12 72 Hughes, J., Astriab, A., Yoo, H., Alahari, S., Liang, ... & Lebleu, B (19 97) A truncated HIV- 1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus J Biol Chem 272, 16 010 16 017 Á 10 Vives, E., ... Chem 2 71, 18 188 18 193 Brugidou, J., Legrand, C., Mery, J & Rabie, A (19 95) The retroinverso form of a homeobox-derived short peptide is rapidly internalised by cultured neurones: a new basis for...

Ngày tải lên: 21/02/2014, 03:20

... tract Antivir Ther 2 011 ;16 (8) :11 49 -11 67 Available at http://www.ncbi.nlm.nih.gov/pubmed/2 215 5899 Recommendations for Use of Antiretroviral Drugs in Pregnant HIV- 1- Infected Women for Maternal Health ... reduce mother-to-child transmission of HIV- 1: NVAZ randomised clinical trial Lancet Oct 11 2003;362(93 91) :11 71- 117 7 Available at http://www.ncbi.nlm.nih.gov/pubmed /14 568737 Taha TE, Kumwenda NI, Hoover ... reduce mother-to-child transmission of HIV- 1: NVAZ randomised clinical trial Lancet Oct 11 2003;362(93 91) :11 71- 117 7 Available at http://www.ncbi.nlm.nih.gov/pubmed /14 568737 Gaillard P, Fowler MG, Dabis...

Ngày tải lên: 05/03/2014, 13:20

... Acad Sci USA 10 0, 411 4– 411 9 50 Ryther RC, Flynt AS, Phillips JA III and Patton JG (2005) siRNA therapeutics: big potential from small RNAs Gene Ther 12 , 5 11 FEBS Journal 277 (2 010 ) 867–876 Journal ... addition, hnRNP E1 ⁄ E2 are also able to interact with an HIV- 1 segment including the ESS3 element in tat ⁄ rev exon of HIV- 1 and modulation of hnRNP E1 expression alters HIV- 1 protein synthesis ... A novel splice donor site in the gag-pol gene is required for HIV- 1 RNA stability J Biol Chem 2 81, 18 644 18 6 51 Purcell DF & Martin MA (19 93) Alternative splicing of human immunodeficiency virus...

Ngày tải lên: 06/03/2014, 09:22

... B-factor (A2)d K103N-Efavirenz K103N-MSC194 K103N-PNU1427 21 MAX-lab beam line 711 MAR-research C22 21 1.0232 11 9.54, 15 7. 31, 15 7 .17 25–3.0 254 669 30 024 96.4 19 795 0 .11 3 MAX-lab beam line 711 MAR-research ... MAR-research C22 21 1.0232 11 9.63, 15 7 .17 , 15 6 .19 25–3.0 48 782 29 948 97.6 16 687 0 .15 2 ESRF beam line BM14 MAR-research C22 21 0.9 31 120.34, 15 6.54, 15 6.47 50–3.0 2 61 528 32 18 8 85.2 24 6 01 0 .11 4 MAX-lab ... line 711 MAR-research C22 21 1. 015 9 11 9.90, 15 6.40, 15 6.90 25–2.8 289 ,12 0 36,534 90.3 27,906 0.079 3 .11 –3.00 2873 97.9 765 3 .11 –3.00 2856 97.8 383 3 .16 –3.09 11 70 73.6 12 4 2.90–2.80 3,306 92.3 1, 488...

Ngày tải lên: 18/03/2014, 01:20