aptamer gold nanoparticle conjugates for the detection of proteins
nanotechnology for cancer therapy, 2007, p.810
... anti-tumor therapy Although the development of nanoplatforms for cancer therapeutics is a critical component to the successful use of nanovectors for anti-tumor therapy and tumor imaging, there are other ... reduction of inherent targeting tendencies for the material(s) used to prepare the nanoparticle Depending upon the physical and chemical nature of the nanoparticle and the mode of administration, there ... In the delivery of chemotherapeutic agents to solid tumors this is particularly critical, since the therapeutic window for these agents is often small and the dose–response curve steep Therefore,...
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Báo cáo khoa học: Protein transport in chloroplasts ) targeting to the intermembrane space pptx
... same concentrations of the precursor form In an effort to address the involvement of known Toc subunits more directly, we expressed the soluble domain of one of the receptor proteins, Toc34, and ... ATP The yield of pTic22 import in the absence of ATP varied between 20–50% of that in the presence of ATP The import of pMGD1 was efficient only at FEBS Journal 274 (2007) 5043–5054 ª 2007 The ... between each of the two and pSSU Another requirement for the import competence of chloroplasts Intermembrane space targeting in chloroplasts for preproteins such as pSSU is the presence of protease...
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Báo cáo khóa học: Fidelity of targeting to chloroplasts is not affected by removal of the phosphorylation site from the transit peptide doc
... Furthermore, the stability of GFP ensures that problems of degradation of mistargeted proteins, which is characteristic of the in vitro system, not occur Ó FEBS 2004 As well as the role of the ... reisolation of the organelles, and the proteins were analysed by SDS/PAGE and fluorography (Fig 2) Figure 2A shows the results for GFP alone The translation product of 27 kDa, corresponding to the size of ... observed for all three constructs From these experiments we can conclude that the presence of the GFP as passenger protein, rather than the mature SSU protein, did not affect the ability of the SSU...
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Báo cáo Y học: The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains pptx
... affect the conformation of native structure Furthermore, bovine serum albumin was required for the renaturation of r-d4, resulting in failure in analysis of the toxin structure On the other hand, ... corresponds to the N-terminal sequence of the intact D4 fragment, indicating that no cleavage occurred On the other hand, the N-terminal amino acid sequence of the digested product of the liposome-bound ... background of the whole h-toxin structure The arrow indicates the position of cleavage by the protease in the presence of cholesterol-containing liposomes The N-terminal sequences of T2¢ and...
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drug targeting, strategies, principles, and applications
... by the ability of the IT to inhibit the incorporation of 3H-thymidine or 3H-leucine into the target cells (29) The potency of the IT is defined as the concentration of IT that inhibits 50% of the ... the IT is therefore expressed as a percentage of the activity of the free antibody The most widely used procedure is to radiolabel both the IT and the antibody and to measure the percentage of ... recognized by the lectin receptor of the reticuloendothelial cells of the liver and spleen and is responsible for the liver toxicity of both RT and RTA (28) Therefore, deglycosylation of RTA is a...
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Nanosized magnetofluorescent fe3o4–curcumin conjugate for multimodal monitoring and drug targeting
... cancer cell hyperthermia Furthermore, to the best of our knowledge, the magnetization values of the fabricated Fe3 O4 fluids are comparable to the best result, reported in literature for magnetic ... In the case of CSF–Cur, IR spectra demonstrated the fingerprint band shift of bending vibration of ı(N–H) from 1638 to 1681 cm−1 , indicating binding of iron ions to NH2 group of CS (Fig 3) Further, ... spectrum of pure Cur, IR spectra of OLF–Cur and CSF–Cur showed a significant change (peak form and position) in the range of 3600–3500 cm−1 , which was assigned to the vibration of –OH group of Cur...
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Báo cáo y học: "Nonimmunoglobulin E–Mediated Immune Reactions to Foods" pptx
... and high levels of specific IgE The clinical removal of the allergens that react with the specific IgE of the patient can decrease AD symptoms in the affected patient.14 The role of T cells is ... tests As opposed to the immediateness of IgE-mediated reactions, the onset of the symptoms of FPIES is delayed from to 10 hours, with a median of hours after the ingestion of food Symptoms typically ... because of the delay in the onset of symptoms after ingestion of the food IgE-mediated reactions typically occur seconds to hours after ingestion of the food and have a clear clinical pattern In the...
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báo cáo khoa học: "Adenovirus-mediated siRNA targeting Bcl-xL inhibits proliferation, reduces invasion and enhances radiosensitivity of human colorectal cancer cells" ppt
... metastasis-related proteins in CRC cells The Bcl-2 family members are important regulators of the mitochondrial pathway of apoptosis Then, we analyzed the effect of siRNA-mediated Bcl-xL inhibition on the expression ... to improve the therapeutic efficacy of radiotherapy for gliomas [30] However, up to date, there have been no reports about the associations of Bcl-xL expression and radiosensitivity of human CRC ... the bottom of the membrane were fixed, followed by staining with 0.05% crystal violet The number of invaded cells on the membrane was then counted under a microscope Flow cytometry analysis of...
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Báo cáo y học: "CD134 as target for specific drug delivery to T cells in adjuvant arthritis" pps
... elevated throughout the active disease phase, indicating a continuous activation of T cells, probably because of the presence of an Mt depot at the base of the tail However, in the PLN the percentage ... expressed as the mean SI for quadruple wells The cut-off value for proliferation was set at SI (indicated by line) may affect the response of auto-aggressive T cells in vivo Optimizing the therapeutic ... interpretation of the results WVE participated in the design of the study and in its coordination LE participated in the experiments, statistical analysis and interpretation of the results MW supervised the...
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Báo cáo y học: "Magnetically retainable microparticles for drug delivery to the joint: efficacy studies in an antigen-induced arthritis model in mice" doc
... Figure These images allowed the calculations of the distance between the magnet and the knee and of the magnetic flux density exerted on the microparticles in the joint, using ViziMag software ... histological analysis, performed on the knees of all of the animals days after the injection, validated the macroscopic observations as well as the results obtained for the uptake of 99mTc Discussion ... than the 1-μm microparticles, we chose the 10-μm microparticles for further therapeutic application The next step was to determine the influence of an external magnet on the i-a retention of this...
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báo cáo khoa học: "Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells" pdf
... combination of saikosaponin and cisplatin for another 30 Cells were stained with DHE (C) or CM-H2DCFDA (D) 30 before collecting cells and then analyzed by flow cytometer Page of the effect of ROS ... However, the effect of combination of saikosaponins and chemotherapeutics has never been addressed In the present study we found that non-toxic dose of either SSa or SSd could sensitize a panel of ... In addition, the cleavage of the caspase-3 substrate PARP (115 kDa) and generation of the small fragment (23-kDa) in the co-treated cells were also significantly Wang et al Journal of Experimental...
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báo cáo khoa học: " The Washington Needle Depot: fitting healthcare to injection drug users rather than injection drug users to healthcare: moving from a syringe exchange to syringe distribution model" pps
... programs For example, the monthly statistics form for needle distribution from the VCH carries an official "performance target" of 90% written at the top of the form The separation of syringe ... lives There was, as a result, some tension between the supporters of the program: the VCH, VANDU and the PHS The hard-line approach of the PHS was in direct contravention of the wishes of VCH ... pharmacies, the requests of IDUs for these life saving items are often turned down [33] As obstacles to syringes for IDUs elevate the risk for the spread of infectious diseases like HIV[13], these...
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Báo cáo sinh học: " Anti-metastatic effects of viral and non-viral mediated Nk4 delivery to tumours" doc
... utilised for Nk4 gene therapy of cancer [9-11], the short lived expression in tumours associated with these vectors may Page of (page number not for citation purposes) Genetic Vaccines and Therapy ... generated by cloning of the Nco1, Xba1 fragment of pGL3 (Promega) containing the firefly luciferase gene, by blunt end ligation in the EcoRI, Xba1 region downstream of the CMV promoter of AAV-MCS cloning ... sections show the morphological appearance of tumours on the inside of the lungs Lungs were harvested from mice at day 21 post tumour induction (e) Average volume of lung metastases The Nk4 group...
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Báo cáo y học: "Custom astrocyte-mediated vasomotor responses to neuronal energy demand" pptx
... PGT-mediated exchange of intracellular lactate for extracellular PGE2 These findings establish lactate as a novel operator of the astrocyte vasomotor switch and are consistent with the ability of lactate ... flow [3], and the current findings by Gordon et al [11] show that vasodilation dominated near the low end of physiological PO2 in brain slices Determination of where the switch point for dilation ... (95%), the same treatment yielded PGE2-dependent vasodilation in slightly hypoxic medium (20% O2) The mechanism for this functional switch is twofold: first, mGluR activation increased the rate of...
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Advanced control strategies for automatic drug delivery to regulate anesthesia during surgery
... anesthetics Inhaled anesthetics are used by many anesthesiologists for the maintenance of anesthesia while the intravenous anesthetics are used at the start of the surgical procedure as they ... performance with the performance of other control structures The second objective of this study is to develop and evaluate the MPC control structure to find the best infusion rates of the anesthetic ... 83 Comparison of the performance of the proposed MPC controller for several patient parameters 87 Comparison of the performance of the MPC, CIMC and CMEC controllers...
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Báo cáo sinh học: " In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter" potx
... of infection (MOI) of 100 in the presence or absence of mM butyrate, a histone deacetylase inhibitor The percentage of GFP positive (GFP+) cells and the levels of expression, represented by the ... containing the human flt-1 promoter is enhanced by the addition of HDAC inhibitors, and the viral genome or proteins coupled to the DNA from the virus are implicated in the silencing of gene expression, ... autocrine-paracrine effect or into the bloodstream for a systemic effect Within the vasculature, endothelial cells are the main target for gene therapy because they are closely related with disease...
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Báo cáo hóa học: " In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter" docx
... of infection (MOI) of 100 in the presence or absence of mM butyrate, a histone deacetylase inhibitor The percentage of GFP positive (GFP+) cells and the levels of expression, represented by the ... containing the human flt-1 promoter is enhanced by the addition of HDAC inhibitors, and the viral genome or proteins coupled to the DNA from the virus are implicated in the silencing of gene expression, ... autocrine-paracrine effect or into the bloodstream for a systemic effect Within the vasculature, endothelial cells are the main target for gene therapy because they are closely related with disease...
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Báo cáo y học: "Blood autoantibody and cytokine profiles predict response to anti-tumor necrosis factor therapy in rheumatoid arthriti" pps
... separation of the actual cytokine values P values are shown for each cytokine The grey bar links the actual responder or nonresponder label of a sample with the logistic regression result of the same ... cross-validation of the dataset was performed; red, overall error of the cross-validation For better readability, error bars are shown for the cross-validation graph only The number of markers is shown ... of the identified markers is not presented in this paper Because of the small sample numbers in the individual cohorts and the fact that the experiments were performed over a 3-year period, the...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... in the mobility of helix A of the protein As Trp13 is located in the vicinity of the activation region AR2 of the protein [1], which is responsible for the activation of the second class of E ... 0.09 for apo-CRP to the value of 0.094 for the CRP(cAMP)2 complex, and no substantial changes in the shape of the emission spectra of the donor have been observed, this justies the lack of the ... Cys178 The dark grey dotted curve shows the intensity decay of the donor alone (D), and the grey dotted curve shows the intensity decay of the donor in the presence of the acceptor (DA) The black...
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Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx
... inactivation of the AMPK by insulin (Fig 7), suggesting a dominance of the fatty acid-driven pathway for activation of AMPK over at least some aspects of insulin signalling This dominance of the fatty ... in all other experiments Although these fed/ starved measurements were closely time-matched with each other they were made some time after all of the perfusion experiments It is therefore possible ... the immunoprecipitates were again recovered by centrifugation and washed three times (4 °C) in 300 lL of AMPK assay buffer before resuspension in 75 lL of the same buffer together with the other...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25