applications of proteomics in prostate cancer

... retroelements (IAPs) Together with our data, these findings highlight the difficulty of avoiding DNA contamination in clinical samples and the risk of testing contaminated samples as XMRV-positive by sensitive ... RNASEL R462Q in our clinical samples (Table 5) Phylogenetic analyses of MLV-like sequences in prostate tissue DNA XMRV has been PCR amplified from prostate cancer samples in a number of studies ... sequences In order to establish consistency and to minimize the risk of contamination during the procedure, three individuals independently performed the nested PCR experiments using independently...

Ngày tải lên: 13/08/2014, 01:20

... overexpressed in a variety of tumor tissues, including prostate cancer AMACR was identified as a tissue biomarker for prostate cancer by gene expression profiling of primary human prostate cancer and ... Immunostaining of prostate cancer tissue with antibodies against AMACR and PSA Immunostaining of prostate cancer tissue with antibodies against AMACR and PSA Surgically resected prostate cancer tissue ... with HLA-A24 binding motif (underlines) The predicted anchor residues to HLA-A24 are indicated in boldface within the amino acid sequences of the peptides B Binding affinities of AMACR-derived...

Ngày tải lên: 18/06/2014, 15:20

... of innate immunity for productive infection in humans XMRV replicates efficiently in prostate epithelial cell lines specifically in LNCaP cells [3,18] In addition, the prostate cancer cell line ... source of infectious XMRV Virus infections were performed using cells plated day before infection Cells were at 50% confluency at the time of infection On the day of infection, fresh media containing ... replication in human peripheral blood mononuclear cells Interestingly, XMRV infects and replicates efficiently in prostate cancer cells of epithelial origin It has been proposed that due to lack off...

Ngày tải lên: 18/06/2014, 18:20

... effect of MDA-7 on prostate cancer cells Studies of adenoviral vectorinduced expression of MDA-7 in human prostate cancer cells demonstrated varying degree of inhibition of growth and induction of ... TGase-4 is linked to the invasiveness of prostate cancer cells [12] and participates in the regulation of the interactions between prostate cancer cells and endothelial cells, the later involving the ... interacting with TGase-4 using immunoprecipitation of proteins from the prostate gland, we identified a small panel of proteins that interacted with TGase-4, including RON (the HGF-like protein receptor)...

Ngày tải lên: 18/06/2014, 19:20

... effect of MDA-7 on prostate cancer cells Studies of adenoviral vectorinduced expression of MDA-7 in human prostate cancer cells demonstrated varying degree of inhibition of growth and induction of ... TGase-4 is linked to the invasiveness of prostate cancer cells [12] and participates in the regulation of the interactions between prostate cancer cells and endothelial cells, the later involving the ... interacting with TGase-4 using immunoprecipitation of proteins from the prostate gland, we identified a small panel of proteins that interacted with TGase-4, including RON (the HGF-like protein receptor)...

Ngày tải lên: 20/06/2014, 03:20

... value concerning the primary detection and the staging of prostate cancer [27] In principle, signal-generation is based on an increased choline metabolism in prostate cancer leading to an increased ... planning in prostate cancer Q J Nucl Med Mol Imag 2009, 53(2):245-268 28 Reske SN, Blumstein NM, Glatting G: [C-11]choline PET/CT imaging in occult local relapse of prostate cancer after radical prostatectomy ... planning of prostate cancer F-18-choline PET and CT scans of ten patients with histologically proven Page of prostate cancer without extra-capsular tumor extension were acquired using a combined...

Ngày tải lên: 09/08/2014, 08:22

... down-regulation of p-Akt may be required for the anti-neoplastic action of small molecule inhibitors of the PI3K/Akt pathway in prostate cancer cells Combined effects of ErPC3 and ionizing radiation in prostate ... paradigmatic intravenously applicable alkylphosphocholine ErPC3 potently induces apoptosis in prostate cancer cells in vitro These findings corroborate earlier reports on high efficacy of ErPC3 in human ... activation of Akt This process is counteracted by the action of the tumor suppressor PTEN which is lost or inactivated in a variety of solid human tumors, including prostate cancer Increased levels of...

Ngày tải lên: 09/08/2014, 09:20

... preclinical study in prostate cancer xenografts, and suggest that the combination of functional MRI parameters, in addition to standard clinical parameters, increases the power of predicting therapeutic ... training, testing and validating the network showed curve flattening after a few training epochs, this indicated that no overtraining occurred However, if such a model is to be applied on clinical ... from a preclinical study in prostate cancer xenografts, indicate that the combination of several functional MRI parameters obtained pre-treatment and early in the course of treatment, into an artificial...

Ngày tải lên: 09/08/2014, 09:20

... Background Quality of life is becoming one of the most significant issues in treatment decision-making, in general, and more so in prostate cancer [1] Thus late rectal and urinary damage became ... toxicity observed in patients during their last inspection are shown In the cohort of patients included in the WP group, pretreatment GI toxicity of grade was found in the history of patients (1.72%), ... recognition of prostate cancer cells Thus we can hypothesize, according to results of Hurwitz et al [12] that the enhanced immune function, involving resting and terminally differentiated NK cells during...

Ngày tải lên: 09/08/2014, 09:20

... use of FCS-containing media during genistein incubation and the incubation with higher genistein doses Mechanisms of interaction To search for mechanisms of interaction we investigated protein ... Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: possible involvement of inhibition of survival signal transduction pathways Int ... after incubation with genistein concentrations of 15 – 30 μM in FCS-containing media [41] In this study the NF-κB activity was investigated, too An inhibition of radiationinduced activation of NF-κB...

Ngày tải lên: 09/08/2014, 09:22

... less Interfraction position variation of the prostate as a source of treatment error is mainly caused by variable fillings of the bladder and/or rectum that displace the prostate mainly in SI ... Jaffray DA, Catton CN: On-line aSi portal imaging of implanted fiducial markers for the reduction of interfraction error during conformal radiotherapy of prostate carcinoma Int J Radiat Oncol Biol ... comparison of the use of bony anatomy and internal markers for offline verification and an evaluation of http://www.ro-journal.com/content/4/1/13 41 42 43 44 the potential benefit of online and offline...

Ngày tải lên: 09/08/2014, 09:22

... accuracy of the bladder scanner Such small differences are unlikely to result in any clinical significance in prostate motion Also of interest is that the difference from baseline PVR urine volumes in ... within a margin of accuracy of ± 20 cc Page of (page number not for citation purposes) Radiation Oncology 2009, 4:26 http://www.ro-journal.com/content/4/1/26 in the range of to 699 ml of urine ... aspect of clinical research in the delivery and treatment of prostate cancer The two organs receiving the greatest scrutiny are the bladder and rectum secondary to the close proximity to the prostate...

Ngày tải lên: 09/08/2014, 10:20

... be defined in current and future clinical trials In Canada, a clinical trial is underway evaluating linac based IMRT and helical tomotherapy, clinically assessing a dose regimen of 68 Gy in 25 ... rest of the pelvis The clinical impact of this difference in terms of acute and late effects is currently unknown Unfortunately, there are no defined dose limits to OARs in the setting of hypofractionated ... targeting is superior to 3DCRT in dose delivery and critical structure sparing in the setting of hypofractionation for high-risk prostate cancer This technical dosimetric feasibility study offers...

Ngày tải lên: 09/08/2014, 10:21

... somatostatin and Octreotide in prostate cancer cell lines To examine the regulatory effects of somatostatin and Octreotide on XAF1 mRNA and protein expression, prostate cancer cell lines (LNCaP, ... features of prostate cancer progression from early stages to androgen independent stages, could mimic the development of prostate cancer clinically Understanding the regulating effects of XAF1 during ... regulating the activities of phosphotyrosine phosphatases [23] In the current study, we examined XAF1 mRNA and protein expression in four cell lines, and determined regulatory effects of somatostatin...

Ngày tải lên: 10/08/2014, 10:20

... therapeutics for prostate cancer candidate predictor of efficacy in both prostate and breast cancer Finally, the observed sensitivity to dasatinib of prostate cancer cell lines with expression of basal ... level of uPA expression is enhanced [34] In addition, RNA interference-induced knockdown of uPA inhibits invasion, survival and in vivo tumorigenicity of prostate cancer cells [35] In the clinic, ... the difficulty in obtaining biopsies from prostate cancer patients It remains to be determined whether the molecular subtypes of prostate cancer identified through molecular profiling mimic the...

Ngày tải lên: 14/08/2014, 08:20

... and binds phosphatidic acid (PA) via a TAPAS domain in its unique N-terminal region An increase in intracellular CA2+ brings about binding of PDE4A1 to PA and insertion into lipid bilayers in an ... domain REC domains play a crucial role in bacterial signalling systems They may also be involved in protein-protein interactions (Galperin 2010) Phosphorylation of the REC domain may be involved ... Signalling in the Prostate and Prostate Cancer 105 1.10.1 cAMP PDEs in Prostate Cancer 105 1.10.2 Protein Kinase A and Prostate Cancer 106 1.10.3 EPAC and Prostate Cancer 108...

Ngày tải lên: 22/12/2014, 21:15

... Clinical Diagnostics Table 4a Interday Validation of A4 in g/L of PBS/BSA Table 4b Intraday Validation of A4 in g/L of PBS/BSA Table 5a Interday Validation of T in g/L of PBS/BSA Table 5b Intraday ... 5b Intraday Validation of T in g/L of PBS/BSA Table 6a Interday Validation of DHT in g/L of PBS/BSA Table 6b Intraday Validation of DHT in g/L of PBS/BSA Table Recoveries of A4, T and DHT at Low, ... Table 14a Interday Validation of A4 in 1g/L PBS/BSA Table 14b Intraday Validation of A4 in 1g/L PBS/BSA Table 15a Interday Validation of T in 1g/L PBS/BSA Table 15b Intraday Validation of T in 1g/L...

Ngày tải lên: 09/09/2015, 08:14

... Detecting cancer- specific peptides in prostate cancer using MHC tetramer technology 12 INTRODUCTION BACKGROUND OF PROSTATE CANCER Prostate cancer is the 5th most common male cancer in Singapore, ... Detecting cancer- specific peptides in prostate cancer using MHC tetramer technology 10 FIGURES PAGE 11 A 77 year old prostate cancer patient with six-fold increase in positive staining intensity of ... for prostate cancer in Singapore Detecting cancer- specific peptides in prostate cancer using MHC tetramer technology 14 Prostate cancer is also the most common non-cutaneous male cancer in North...

Ngày tải lên: 05/10/2015, 18:59

... allows the determination of in vivo protein binding using microdialysis sampling in blood and simultaneous blood sampling [33,34] The in vivo determination of protein binding using the microdialysis ... determination of the temporal course of protein binding in the same animal to determine saturation of the plasma protein binding [33] The utility of microdialysis sampling for estimation of the in ... connector Inlet (A) (B) Double tubing Membrane Inlet Steel shaft Outlet Membrane Introducer needle Inlet Outlet Introducer Needle Suture point Tubing From bile duct into small intestine Inserted into...

Ngày tải lên: 05/03/2014, 22:21