applications of mass spectrometry to analyze structure and bioactivity of chitooligosaccharides

... structures of these proteins, all tetrameric, showed significant similarity to the published structures of transthyretin By way of example, a comparison of the structure of S dublin TLP with the structures ... in TLP structures as a result of alterations to the formation of hydrogen bonds between strands The carbonyls of residues V104 and P105 (zebrafish TLP numbering), in the middle of b-strand G, ... S dublin TLP and zebrafish TLP are found in the flexible portions of strands B and C that protrude towards the solvent and in the conformation of the long loop connecting strands D and E [17] Greater...

Ngày tải lên: 07/03/2014, 00:20

... THE PROGRESSION OF DM The first three of these historical phases of industrial DM serve to summarize and rationalize the scientific questions of yesteryear and today The questions of tomorrow are ... PART II MASS SPECTROMETRY IN DRUG METABOLISM: PRINCIPLES AND COMMON PRACTICE Theory and Instrumentation of Mass Spectrometry 255 257 Ge´rard Hopfgartner Common Liquid Chromatography Mass Spectrometry ... recent applications of imaging mass spectrometry for these studies are described Novel instrumentation and mass spectrometry scan functions of hybrid triple quadrupole–linear ion trap mass spectrometry...

Ngày tải lên: 28/03/2014, 10:20

... Number of unique reections Temperature (K) Rfactor (%) Rfree (%) Rmsd bond lengths (A) Rmsd bond angles () Number of all atoms Number of protein atoms Number of waters Number of inhibitor atoms ... the study of protein structure and function Of particular interest is the use of ESIMS in the studies of noncovalent interactions [5254], as valuable parameters such as stoichiometry and binding ... and [L] are the concentrations of the free protein and free ligand (i.e inhibitor), respectively [68] Expressed in terms of r, dened as the number of ligands bound to one protein molecule, Eqn...

Ngày tải lên: 07/03/2014, 17:20

... target (Opti-TOF; Applied Biosystems) MALDI-TOF-TOF MS and MS ⁄ MS spectra were acquired with a MALDI-TOF ⁄ TOFÔ 4800 mass spectrometer (Applied Biosystems) in the positive and reflector mode An ... using the available softwares (gpmaw [30], xquest [31] and msx-3d [12]) with a mass tolerance of p.p.m We therefore used MALDITOF-TOF and ⁄ or nanoLC-Orbitrap tandem MS to further identify peptides ... (A) MALDI-TOF-TOF mass spectrum of the light (d0) and heavy (d4) precursor ions presenting a mass difference of 4.024 Da (indicated by the curved arrow) (B) Fragmentation mass spectrum of the double-charged...

Ngày tải lên: 15/03/2014, 00:20

... plate (Applied Biosystems) 2.2.6 MALDI TOF/TOF MS/MS Analyses An ABI 4800 Proteomics Analyzer MALDI TOF/TOF mass 15 spectrometer (Applied Biosystems) was used to analyze the samples on the MALDI target ... Silver staining of different amount of BSA picomol, 500 femtomol, 100 femtomol, 50 femtomol, 10 femtomol, femtomol to femtomol BSA (From left to right)were loaded and run by SDS-PAGE and then stained ... region of the mass spectrometer known as the mass analyzer The mass analyzer is used to separate ions within a selected range of mass- to- charge (m/z) ratios The analyzer is an important part of the...

Ngày tải lên: 04/10/2015, 15:46

... of transmitting power and control to the hand If standards were defined, quick-change mechanisms and a family of hand grippers and robot tools would rapidly become available The development of ... www.Abika.com 32 APPLICATIONS OF ROBOTICS AND ARTIFICIAL INTELLIGENCE • • • • how to capture and deploy knowledge and duties of the operations, intelligence, logistics, and fire-support officers into operations, ... Manipulator and Actuation Research on the kinematics of design, models of dynamic behavior, and alternative design structures, joints, and force programming is leading to highly accurate new robot structures...

Ngày tải lên: 13/12/2013, 01:15

... labeling mass spectrometry, a total of 438 proteins were identified by our proteomic platform To better understand the roles of these identified proteins, they were grouped and analyzed according to ... Interestingly, Clostridium perfringens enterotoxin is a ligand for CLDN3 and CLDN4 proteins, and binding of the toxin to these claudins leads to rapid cytolysis of cells [26] Preclinical studies have ... quantitation, we used multi-q [41] software to analyze the iTRAQ data Raw data files from the Waters Q-Tof premier mass spectrometer were converted into files of mzXML format using masswolf (Institute for...

Ngày tải lên: 16/02/2014, 15:20

... of AAP The pH was adjusted to 2.5, 8.0 and 11.5 and the samples were incubated at 37 °C, 50 °C and 70 °C Aliquots were taken at different time-points from each sample and analyzed by MALDI-TOF ... (M23L) and rONC was taken from a previous publication [20] Curves were normalized to facilitate comparison of the unfolding transitions induced by temperature Cytotoxicity assays MALDI-TOF MS All mass ... recombinant protein, the conversion of reagents and products of the exopeptidasecatalyzed hydrolysis of Met1 and cyclization of Gln1 to pyroglutamyl were monitored at different time-points during...

Ngày tải lên: 19/02/2014, 12:20

... as the b-anomer of Gal II H1 gives an rOe to this proton Gal II is a branching point and substituted at positions and 6, as GlcNAc III H1 gives an rOe to H6a and H6b of Gal II, and GlcNAc VII ... the 6-branch is substituted at positions and 4, as Gal IV H1 gives an rOe to H4 of GlcNAc III, and Fuc IX H1 gives an rOe to H3 and to the NAc protons of this residue The trisaccharide, Gal IVb1– ... shows an rOe to H3 of Gal IV GlcNAc V is substituted at the 3- and 4-position, as Gal VI H1 gives an rOe to GlcNAc V H3 and to the NAc protons of GlcNAc V, while Fuc X H1 gives an rOe to GlcNAc...

Ngày tải lên: 19/02/2014, 12:20

... of pleABD/2a as found in structures I and II is stable and not subject to conformational changes due to different crystal packing Quality of protein structure models The final R and Rfree factors ... atoms in superpositioned structures (B) are plotted as a function of residue numbers IA, structure I molecule A; IB structure I molecule B; II, structure II; HP, structure of human plectin and ... statistics of both structures, average temperature factors, and target deviations against stereochemical restraints are given in Table For visualization and rebuilding of the structures O and XTALVIEW...

Ngày tải lên: 07/03/2014, 15:20

... carbon atoms of pyruvate, the mass isotopomer distribution of which is reflected in this of alanine Therefore, the mass isotopomer distribution of Glu can be estimated from the mass isotopomer ... carbon atoms of OAA/Asp become the first three carbon atoms of aKG/Glu (see Fig 7), while the carbon atoms of acetyl-CoA (AcCoA) become the last two carbon atoms of aKG/Glu The carbon atoms of AcCoA ... The mass isotopomer measurements of threonine and methionine validated this assumption Neither the mass isotopomer distribution of threonine nor that of methionine indicated the presence of isotopic...

Ngày tải lên: 08/03/2014, 08:20

... d(G4C4), possesses composite features of both A and BDNA In view of these, it is perhaps appropriate to regard the structure of iso d(G4C4) as a hybrid structure of A and B forms It is gratifying that ... Chattopadhyaya, J (1994) Quantitation of anomeric effect in adenosine and guanosine by comparison of the thermodynamics of the pseudorotational equilibrium of the pentofuranose moiety in N- and ... to 18 ps of high-temperature dynamics followed by slow cooling to 100 K, at steps of 50 K During each cooling step the structures were subjected to 500 fs of molecular dynamics Finally, the structures...

Ngày tải lên: 16/03/2014, 18:20

... 10-80% of the base peak Low -mass sensitivity 68 50% of Mass 198 High -mass resolution and sensitivity 443 15-24% of Mass 442 High-mass...

Ngày tải lên: 22/03/2014, 14:20

... quality of telephone-band (300-3400 Hz) and wide-band speech signals, while ITU-R Task Group 10/4 deals with the quality of speech and music signals in general 30 APPLICATIONS OF DSP TO AUDIO AND ... changes and improvements to the perceptual model even after a standard is fixed and a lot of decoders are deployed at the customers Perceptual models 60 APPLICATIONS OF DSP TO AUDIO AND ACOUSTICS ... introduction to the physical and perceptual aspects of reverberation The remainder of the chapter treats the different types of artificial reverberators known today The main quest in this topic is to generate...

Ngày tải lên: 22/03/2014, 23:20

... boundaries of quotations and inserted clauses as well as sentence boundaries is sensitive to the accuracy of dependency structure analysis and the improvements of the boundary detection of quotations and ... 2: Dependency structures of bunsetsus to left of beginning of quotations or inserted clauses For instance, “と思う, to- omou” (think) and “って言う, tte-iu” (say) are used at the ends of quotations Expressions ... structure analysis based on automatic detection of quotations and inserted clauses Dependency Structure Analysis and Detection of Quotations and Inserted Clauses The outline of the proposed processes...

Ngày tải lên: 23/03/2014, 18:20

... may be further subdivided into three classes (see Figure 1.6a, b): linear TOF, reflectron TOF (reTOF), and orthogonal acceleration TOF (oaTOF) The linear TOF is the first and hence “classic” design, ... xxii TDC TGA TIC TOF VOC WBA ACRONYMS time -to- digital converter thermogravimetric analysis total-ion chromatogram time of flight (oaTOF—orthogonal acceleration TOF; reTOF—reflectron TOF) volatile organic ... with TOF analyzers became very common, as the unlimited upper mass range of TOF meshed well with the high -mass ions and pulsed-ion generation characteristic of MALDI Through it all, TOF mass...

Ngày tải lên: 28/03/2014, 10:20

... HAMLET Cytotoxicity of ELOA complexes Similar to HAMLET, the assembly of equine lysozyme and oleic acid into ELOA complexes led to cytotoxic activity ELOA effectively reduced the viability of mouse ... shown to interact with mitochondria, causing mitochondrial swelling and loss of mitochondrial membrane potential [48,49], accompanied by cytochrome c release, proapoptotic caspase activation and ... acetylation and deacetylation of the histone tail Histone deacetylases, which close the chromatin, are often over-expressed in tumor cells and histone deacetylase inhibitors are therefore used to treat...

Ngày tải lên: 29/03/2014, 21:20

Ngày tải lên: 30/03/2014, 01:20

... turn our standpoint and take natural categories of nouns (concepts) as a base and associate to it through case relation pairs of a verb and its translation equivalent Let a structure of natural ... noun's relation to human becomes closer and the more the level of abstract of the noun becomes lower the numbers of verbs that are closely related to them ~id therefore have to associate to them (nouns) ... conceptual category, ,numbers of associated pairs of verb and its translation equivalent are generally small and can easily be found (3) Association of the pair of verb and its translation equivalent...

Ngày tải lên: 31/03/2014, 17:20

... combination of quadrupoles with TOF instruments are also used to perform tandem mass spectrometry in space (Hoffmann and Stroobant 2007) Tandem Mass Spectrometry –– Applications and Principles Higher order ... combine electric and magnetic sectors (E and B) or electric and magnetic sectors and quadrupoles (E, B and qQ) Time -of- Flight (TOF) instruments equipped with a reflectron or a combination of quadrupoles ... in describing tandem mass spectrometry As such, besides being the father of mass spectrometry, Thomson can also be considered the forefather of tandem mass spectrometry In fact, to demonstrate...

Ngày tải lên: 31/03/2014, 19:20