applications of htrf for high throughput screening
báo cáo khoa học: " A high-throughput screening system for barley/powdery mildew interactions based on automated analysis of light micrographs" docx
Ngày tải lên :
12/08/2014, 05:20
... before detailed images of these regions can be generated A naive complete high- resolution scanning is out of the question due to the tight time constraints of a high- throughput system Therefore, ... susceptibility indices for each slide in terms of the number of infected transformed cells over the total number of transformed cells according to Equation (1) Page of (page number not for citation purposes) ... according to Equation (1) for each scanned slide in terms of the number of infected transformed cells and the total number of transformed cells The detailed description of necessary image processing...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
Ngày tải lên :
07/03/2014, 12:20
... demonstrated for each of the 19 nonproline residues [18] Time and expense can be drastically reduced by use of cell-free systems [11,18,21], opening many avenues for strategic applications of selectively ... accessible This allows the synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced insoluble ... can be produced in high yields in vivo are also suitable for efficient production by cell-free synthesis In order to compensate for the increased effort and expense required for the production...
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Báo cáo sinh học: "Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" doc
Ngày tải lên :
18/06/2014, 18:20
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... modeling enables high- throughput analysis of complex nonlinear systems in various applications [1-2] In particular it was shown that generalized models can be used to obtain statistically highly-significant...
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Báo cáo hóa học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" pdf
Ngày tải lên :
20/06/2014, 01:20
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... modeling enables high- throughput analysis of complex nonlinear systems in various applications [1-2] In particular it was shown that generalized models can be used to obtain statistically highly-significant...
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Báo cáo toán học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" ppt
Ngày tải lên :
20/06/2014, 21:20
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... modeling enables high- throughput analysis of complex nonlinear systems in various applications [1-2] In particular it was shown that generalized models can be used to obtain statistically highly-significant...
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Báo cáo y học: "miRTRAP, a computational method for the systematic identification of miRNAs from high throughput sequencing data" pptx
Ngày tải lên :
09/08/2014, 20:21
... for the discovery of novel miRNA genes A computational approach called miRTRAP (miRNA Tests for Read Analysis and Prediction) was developed for the systematic prediction of miRNAs from high- throughput ... miR Our approach for the identification of microRNAs using high- throughput sequencing reads is to compute a set of quantities for each candidate locus, and by using thresholds for each quantity ... regions of unannotated alternative splicing variants Discussion We have presented a new computational method for the systematic identification of miRs using high- throughput sequence information...
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Báo cáo y học: "ISsaga is an ensemble of web-based methods for high throughput identification and semiautomatic annotation of insertion sequences in prokaryotic genomes" pdf
Ngày tải lên :
09/08/2014, 22:24
... For example, for an IS composed of two ORFs, ISsaga will extract the nucleotide sequence starting from the coordinates of the beginning of the first ORF to the coordinates of the end of the second ... introduced a number of rules that operate automatically to remove many of the major annotation ambiguities encountered due to the diversity and complexity of ISs (for example, the presence of more than ... Exploitation of ISsaga Genome context One useful feature of ISsaga is that it supplies the genome context (that is, flanking genes) for each annotated Page of IS, allowing identification of IS-induced...
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Báo cáo y học: "Establishment of a novel CCR5 and CXCR4 expressing line which is highly sensitive to HIV and suitable for high-throughput evaluation of CCR5 and CXCR4 antagonists" pdf
Ngày tải lên :
13/08/2014, 13:20
... vitro models is a prerequisite for the successful setup of an accurate screening program for chemokine receptor antagonists Here, we Page of 13 (page number not for citation purposes) Retrovirology ... concentrations (IC50) of SCH-C for inhibition of LD78β-induced calcium flux were ng/ml in the single and ng/ml in the double-transfected cells For AMD3100, the IC50 values for inhibition of SDF-1-induced ... after infection of cells The coreceptor use of each isolate is shown between brackets The data of one representative experiment out of two are presented Page of 13 (page number not for citation...
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Development and application of advanced proteomic techniques for high throughput identification of proteins
Ngày tải lên :
16/09/2015, 08:30
... AND APPLICATION OF ADVANCED PROTEOMIC TECHNIQUES FOR HIGHTHROUGHPUT IDENTIFICATION OF PROTEINS HU YI (B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES ... Activity-based high throughput screening of enzymes using DNA microarray Angew Chem Int Ed Engl 44, 1048-1053 (2005) Hu, Y., Uttamchandani, M & Yao, S.Q Microarray: a versatile platform for highthroughput ... different aspects for high- throughput identification of enzymes and their associated proteins in yeast proteome (catalomics) Firstly, to validate the high- throughput capacity of differential gel...
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The Meaning of Marketing for High-Tech Firms
Ngày tải lên :
24/10/2013, 07:20
... very often the case The Meaning of Marketing for High- Tech Firms The Irresistible Rise of High- Tech Services A revolution is at work in the high technology industry: the irresistible growth of ... programmed for each customer), or specific software developed for the particular needs of a customer This distinction between the different types of high- technology products is of importance for a ... challenge for a high tech firm is how to structure the development activities before the full potential of one technology and of its market appeal is established 1.2 What is a high- tech product? 15 For...
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high throughput screening, methods and protocols
Ngày tải lên :
11/04/2014, 09:46
... internalization as well as a range of other applications (6) High Throughput Screening Assays 3.3 Matching Assay Method to Target Type Often, one has a choice of assay method for a given target type (Table ... Saussy, D L (1996) Experimental design for high- throughput screening Drug Discovery Today 1(7), 277–286 Major, J S (1995) Challenges of high throughput screening against cell surface receptors ... Fluorescence Polarization Assays for high throughput screening of G protein-coupled receptors J Biomol Screening 5(3), 159–167 14 Allen, M., Reeves, J., and Mellor, G (2000) High throughput fluorescence...
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![bioinformatics for high throughput sequencing [electronic resource]](https://media.store123doc.com/images/document/14/y/gu/medium_Yb8L87hXir.jpg)
bioinformatics for high throughput sequencing [electronic resource]
Ngày tải lên :
29/05/2014, 14:33
... Bioinformatics for High Throughput Sequencing Naiara Rodríguez-Ezpeleta Ana M Aransay ● Michael Hackenberg Editors Bioinformatics for High Throughput Sequencing Editors ... approaches such as microarrays, furthermore offering finer outcomes A review of each of the applications of high- throughput sequencing in the context of specific research areas is presented in Chap ... range of high- throughput sequencing applications together with the falling cost for generating vast amounts of data suggests that these technologies will generate new opportunities for software...
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Báo cáo y học: "A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language" ppt
Ngày tải lên :
09/08/2014, 22:23
... accuracy Therefore, we always set the threshold for signal-to-noise ratios of peaks at the value of 3.0, which is a commonly accepted estimate for calculating the limit of detection (LOD) of analytical ... University of Dresden and University of Heidelberg for valuable discussions and betatesting of LipidXplorer software; to Mrs Kathy Eisenhofer and Dr Christer S Ejsing (University of Southern ... time -of- flight mass spectrometer [16] The interpretation of the MPIS dataset by LipidProfiler software confirmed 36 species representing 95% of the species identified manually The intersection of...
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báo cáo khoa học: " HAPIscreen, a method for high-throughput aptamer identification" pptx
Ngày tải lên :
11/08/2014, 00:23
... candidates Page of 10 Given the increasing demand for aptamers [13] it would therefore be more efficient to screen directly for the desired property i.e affinity of the target for a candidate ... demonstrate the interest of HAPIscreen (High throughput APtamer Identification screen), an AlphaScreen ® -based method for the detection of aptamers targeted to different RNA hairpins of eukaryotic or ... molecules of interest respectively immobilized on the two beads [20] (Figure 2A) Therefore monitoring the emission signal allows for the detection of complex formation We used this technology for monitoring...
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báo cáo khoa học: " SNP high-throughput screening in grapevine using the " docx
Ngày tải lên :
12/08/2014, 05:20
... 0.32 μM of primer and μl of BigDye Terminator in a final volume of 10 μl Sequencing reactions were performed using a initial denaturation step, followed by 40 cycles at 96°C for 10 sec, 50°C for ... few low- and mid -throughput methods have been used for SNP discovery and genotyping in grapevine (Vitis vinifera L.) [9-12] The sequencing of the highly heterozygous genome of Pinot Noir [13], ... unexpectedly high Table 2: Comparison between gDNA and WGA-DNA SNPlex genotyping assay gDNA Number of genotypes Number of SNPset Number of eSNPs Failed assay Passed assay Average call rate Number of successful...
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Báo cáo y học: "A method for high-throughput gene expression signature analysis" ppt
Ngày tải lên :
14/08/2014, 16:21
... were highly correlated, with 97.9% of data points falling within twofold of their corresponding means (Figure 2) Much of the variability was explained by a single transcript, accounting for 34% of ... of variation of mean expression level for each of the 90 features across all three independent evaluations had a mean of 13.8% (range 1.1-49.8%) indicating high stability of the platform LMF makes ... makes possible a wide variety of gene expression-based applications that have the potential to be transforming, particularly in the area of small molecule screening Highthroughput small molecule...
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Design and characterization of interposers for high speed fine pitch wafer level packaged device testing
Ngày tải lên :
04/10/2015, 10:25
... selection of materials were important as well It often involved a lot of trade-offs between mechanical and electrical performance However, meeting the requirements of being capable of probing ... was found to have limited bandwidth of GHz and an insertion loss of 12 dB at GHz, the target high- speed I/Os of the specifications Therefore, it is not suited for the required wafer level packaged ... Parametric variation study of the interposer is performed for the rest of the chapter Chapter 5: The limitations of the proposed interposer designs are discussed Suggestions for possible improvements...
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Báo cáo khoa học: TICL – a web tool for network-based interpretation of compound lists inferred by high-throughput metabolomics doc
Ngày tải lên :
16/03/2014, 01:20
... KEGGanim: pathway animations for high- throughput data Bioinformatics 24, 588–590 Antonov AV & Mewes HW (2006) Complex functionality of gene groups identified from high- throughput data J Mol Biol ... the share of random simulations where the size of the inferred models for random compound lists of size N are equal to or greater than the size S1, S2, , Sz of the inferred models for input ... amount of data per experiment, high- throughput technologies also brought the challenge of translating such data into a better understanding of the underlying biological phenomena A number of tools...
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