application of recombinant technology to cell based hts

... cloned into TOPO TA vector (Fig 1), and then subcloned into an expression vector pQE30 Expression of rpIL-2 was induced by the addition of IPTG to the bacterial culture (Fig 2) Fig Cloning of porcine ... reached an OD600 of 0.6, the expression of IL-2 was induced by the addition of IPTG into the bacterial culture to a final concentration of 1mM After h of culture in a shaking incubator at 185 rpm, ... cloned into TOPO TA cloning vector (Invitrogen, Carlsbad, CA) producing pTopoIL-2 The identity of the IL-2 gene was confirmed by DNA sequencing and restriction enzyme analysis Expression of recombinant...

Ngày tải lên: 07/08/2014, 15:20

... to be involved in the phagocytosis and entry of virulent M tuberculosis into host macrophages and suppression of their immune response A comparison of phagocytosis of beads coated with LAMs of ... covalently bound to the cell wall (131) However, the ability of antibodies to bind LAM on the surface of intact mycobacteria and the role of LAM in mannose receptor driven phagocytosis suggests ... incorporation of free LAM into the cell membrane of the infected host cell (142) In addition, other immunosuppressive effects of ManLAM have been observed, including modulation of cytokine secretion...

Ngày tải lên: 10/09/2015, 09:05

... APPLICATION OF ION IMPLANTATION TO THE FABRICATION OF GAN -BASED DEVICES WANG HAITING (M Eng., XJTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ELECTRICAL ... stopping and electronic stopping typically [Sze1998], the mechanism of energy loss of an incident ion is a combination of both the stopping mechanisms The ability of the target material to stop ... “sea” of both valence and core electrons of the host atoms Much of the lattice space is composed of this cloud of electrons and many of these interactions will occur The inelastic property of the...

Ngày tải lên: 11/09/2015, 14:24

Ngày tải lên: 23/06/2016, 20:49

... Supelco, Mỹ), tốc độ dòng chảy 1ml/min, Detector l Detector đo số khúc xạ - RI KếT QUả NGHIÊN CứU V THảO LUậN 3.1 Xác định hoạt tính enzyme -D fructofuranosidase chế phẩm pectinex Ultra SP-L v ... hoạt tính enzyme -D frutofuranosidase đ đợc xác định Số đơn vị hoạt tính 1ml dung dịch chế phẩm enzyme l 58,1 U/ml Do chế phảm pectin ex Ultra SP-L chứa enzyme -D frutofuranosidase, sử dụng chế ... fructose đợc vận chuyển tạo FOS đạt giá trị cao 10 H m lợng (%) Fructose vận chuyển 25 30 35 40 45 50 Nhiệt độ (0C) Hình ảnh hởng nhiệt độ đến khả vận chuyển Fructose 10 Hàm lợng (%) Fructose...

Ngày tải lên: 29/08/2013, 08:16

... conditions of 250 oC and MPa, biodegradability of DCAA increased to 0.54 within min, and did not vary much up to reaction time of 57 In case of TCAA, the biodegradability change was similar to that of ... the number of attached chlorine atoms, regardless of the concentration of refractory parts in CAAs The results could be applied as basic data to the treatment of Chlorinated refractory pollutants ... future, further inspection of hydrothermal reaction is also required to clarify reaction mechanism of other kinds of refractory pollutants and to apply this technology to real wastewater ACKNOWLEDGMENTS...

Ngày tải lên: 05/09/2013, 08:40

... participation of others CL offers many ways of promoting equal participation in groups Two of these are the use of rotating roles in a group, such as facilitator, checker (who checks to see that ... arrangement of the classroom, assigning students to groups and roles, and selecting materials and time An important role of the teacher is that of facilitator of learning In his or her role as facilitator, ... difficult to understand and produce words, idioms and phrases of colloquial language • Stress, rhythm and intonation: Learners of English often find it difficult to pronounce English words, to stress...

Ngày tải lên: 29/01/2014, 10:58

... ranking of a web page is given by the weighted sum of the rankings of every other web page, where the weights on a given page are small if that page points to a lot of places The vector of weights ... characterization of actual flows in terms of a network Data The data set used in the study consists of all Tranche transactions in the LVTS from October 1st 2005 to October 31st 2006 This data set consists of ... level of initial liquidity bank is over times more likely to hold onto liquidity sent to it than bank 11, and hence bank holds more liquidity over the course of the day Returning to the Sutton...

Ngày tải lên: 16/02/2014, 12:20

... way to bring about a goal from a range of alternatives The limitation of this stage of experiencing the agentive self is one of physicality Experimental studies of infants towards the end of their ... history of aggression, but there is no evidence of medical complications, hyperactivity or substance abuse in their histories I not wish to deny the importance of the above descriptive indicators ... nine months of age (Tomasello 1999) infants begin to differentiate actions from their outcomes and to think about actions as means to an end This is the beginning of their understanding of themselves...

Ngày tải lên: 16/02/2014, 22:20

... the results ofof the global monitoring of progress towards MDG target C: C: to halve, by 2015, results the global monitoring of progress towards MDG 7 target to halve, by 2015, results of the global ... Ghana) or lack indicators to assess performance of the instruments (China) This lack of monitoring tools to assess the performance of indicators will continue to impact the ability to measure progress ... of the global monitoring of progress towards MDG 77target C: to halve, by 2015, the global monitoring of progress towards MDG target C: to halve, by 2015, the proportion ofof the population without...

Ngày tải lên: 21/02/2014, 12:20

... thnh v trỡ biofloc ao nuụi tụm i T l N/P T lờ N/P quyt nh thnh phn to (t dng) hay vi khun d dng chim u th ao N/P < 10, dinoflagellata (to giỏp) chim u th N/P = 10-20, to lc, to silic, to giỏp v ... Thnh phn biofloc bao gm: Hn hp cỏc vi sinh vt d dng (vi khun to floc v vi khun si), mnh vn, keo, polymer sinh hc, cation, t bo cht, mui tinh th, Bỏm vo biofloc cũn cú vi to (to si, to silic), ... quy trỡnh Biofloc quỏ dy: Loi b bt biofloc bng cỏch thay nc hoc cho nc chy trn qua ng chng trn Biofloc gim, nc cú mu xanh lỏ cõy: Khụng pha loóng nc 5-6 ngy, dit to lam hoc to lc Biofloc gim,...

Ngày tải lên: 07/03/2014, 18:21

... employed in this probe ESR: to 20 ml of benzyl alcohol a small amount of a TEMPO trap was added and the sonication took place under identical conditions to those of the synthesis of the metal oxides ... possible mechanism is related to radicals involved in the reaction as a result of the bubble’s collapse Application of ultrasound to chemical processes involves the use of acoustic cavitation Acoustic ... precursor to form metal–oxygen–metal groups (Scheme 2, Eq 2) The combination of these two equations leads to the elimination of R–X and H–X The other possibility is the elimination of only hydrogen...

Ngày tải lên: 20/03/2014, 13:11

... fractions of 900 µl were collected during the run, corresponding to a total of 720 fractions for each experiment Aliquots of 200 µl of each fraction, corresponding to approximately 20 µg of protein, ... History of myocardial infarction No 50 100.0 History of angina No Yes 47 94.0 6.0 History of coronary artery bypass graft/percutaneous transluminal coronary angioplasty No 47 100.0 History of ... indicative of a substantial effect of CEE on the serum proteome, based on a systematic, unbiased analysis It was of interest to determine the contribution of EREs to upregulation of protein levels...

Ngày tải lên: 28/03/2014, 14:20

... idea of counting word lengths to judge the authorship of texts, followed by Yule (1939) and Morton (1965) with the use of sentence lengths In this paper, we measure the overall byte length of SMS ... non-spammers often use special characters to create emoticons to express their mood, such as “:-)” (smiling) or “T T” (crying), whereas spammers tend to use special character or patterns related to monetary ... significantly lessened) In order to evaluate the contribution of different types of stylistic features, we conducted a series of experiments by removing features of a specific type at a time from...

Ngày tải lên: 31/03/2014, 00:20

Ngày tải lên: 28/04/2014, 10:16

... THE APPLICATION OF HYPERBOLIC FUNCTIONS TO WIRE TELEPHONY 112 !X X 86 THE APPLICATION OF HYPERBOLIC FUNCTIONS TO WIRE TELEGRAPHY 179 HYPERBOLIC APPLICATIONS OF MISCELLANEOUS FUNCTIONS TO ... permits of tfre extension of the " " notion of an angle from the circular to the hyperbolic sector The conception of the "hyperbolic angle" of a continuous(2) That there is ÆTHE ORCE RF PREFACE TO ... that each of the sectors contains, and each of the arcs subtends, for ; a hyperbolic angle of 0*2 hyp.; while the total sector AOF contains, and the arc ABCDEF subtends, a hyperbolic angle of hyp...

Ngày tải lên: 04/06/2014, 12:30

... fragment of tobacco mosaic virus (b) 1.5 & 2.1 kb fragments of tobacco etch virus (c) 1.5 & 2.1 kb fragments of potato virus Y In all cases, although generating signals of lower strength compared to ... virus; TYLCV – tomato yellow leaf curl virus; TMV – tobacco mosaic virus; TEV – tobacco etch virus; PVY – potato virus Y EAB555/F &EAB555/R: PCR conditions consisted of 30 cycles of 94°C for min, ... were carried out to further quantify the ability of FTA technology to fix, store and release geminivirus genomic components Recombinant plasmid DNA carrying the B component of EACMCV [8] in quantities...

Ngày tải lên: 19/06/2014, 08:20

... Another way of examining cross-hybridization of the mouse liver mRNA would be to apply the mouse mRNA to the microarray in the absence of human mRNA However, the purpose of this study was to determine ... transplanted with hepatocytes from different donors, facilitating the analysis of host-specific responses to HCV Global gene expression profiling, in which the expression levels of thousands of genes are ... amount of mouse liver mRNA Only the gene expression in human hepatocytes is of interest as it is only these cells, not the mouse hepatocytes, which are infected with HCV In addition, the percent of...

Ngày tải lên: 20/06/2014, 01:20

... fragment of tobacco mosaic virus (b) 1.5 & 2.1 kb fragments of tobacco etch virus (c) 1.5 & 2.1 kb fragments of potato virus Y In all cases, although generating signals of lower strength compared to ... virus; TYLCV – tomato yellow leaf curl virus; TMV – tobacco mosaic virus; TEV – tobacco etch virus; PVY – potato virus Y EAB555/F &EAB555/R: PCR conditions consisted of 30 cycles of 94°C for min, ... were carried out to further quantify the ability of FTA technology to fix, store and release geminivirus genomic components Recombinant plasmid DNA carrying the B component of EACMCV [8] in quantities...

Ngày tải lên: 20/06/2014, 04:20