apoptosis anoikis and senescence

... pathway-induced senescence correlates with increased b-galactosidase activity and induction of classical senescence- associated genes, such as p16 ⁄ INK4A, p53, p21 and p14-p19 ⁄ ARF (Table 2), senescence- associated ... between RasV12-, RasR61- and B-RafE600-induced senescence in human melanocytes suggests that the senescence programs are different: Ras-induced senescence was faster and was associated with massive ... ª 2009 The Authors Journal compilation ª 2009 FEBS ERK and cell death S Cagnol and J.-C Chambard Table Models of ERK-mediated apoptosis and autophagy LDH, lactate dehydrogenase; MDC, monodansylcadaverine;...

Ngày tải lên: 29/03/2014, 08:20

... maturation senescence proand estimate cesses, therebyeliminatingthe need to arbitrarily age at senescence We fit and compared4 variationsof a general model describing reproduction,maturation, and senescence, ... describingthe processes of maturationand senescence Ursus 14(2):109-119 (2003) REPRODUCTIVE MATURATION SENESCENCE Schwartzet al AND * 111 Table Geographic area, years of study, and sample size (n = 4,726 ... sampled Females observed (n) Reference 1985-99 1984-99 199 177 and Bjarvall Sandegren (1987) and Bjarvall Sandegren (1987) KodiakIsland BlackLake Game ManagementUnit13 Katmai NationalPark DenaliNationalPark...

Ngày tải lên: 05/03/2014, 17:20

... Mechanisms of Cellular Senescence Therese Becker and Sebastian Haferkamp Section Plant Senescence Chapter Plant Senescence and Nitrogen Mobilization and Signaling 53 Stefan Bieker and Ulrike Zentgraf ... and senescence [71, 72] and this requires functional p16INK4a [73] The best understood transcription factors driving p16 INK4a expression and thereby growth ar‐ rest and senescence are Ets1 and ... are essen‐ tial to initiate and maintain senescence and it is not surprising that all three molecules are considered important tumor suppressors 27 28 Senescence and Senescence- Related Disorders...

Ngày tải lên: 23/03/2014, 05:21

... amphiregulin and bFGF 269 269 273 273 274 277 Summary 278 References 279 14 Senescence and immortalization of human Cells 281 Karen Hubbard and Harvey L Ozer Introduction 281 Cellular replicative senescence ... differentiation between G2 and M and between post-mitotic and pre-synthetic G1 (from ref 5) Equipment and reagents • • • BrdU PBS RNase A 0.1 M HCI PBST: ml ice-cold PBS and 0.4% Tween-20 13 • ... and Zbigniew Darzynkiewicz Introduction 145 Cyclins D, E, A, and B1 and Cdk inhibitors (Ckis) 146 Expression of cyclins D, E, A, and Bl and Ckis detected by flow cytometry 151 Critical steps in...

Ngày tải lên: 10/04/2014, 11:13

... moves towards senescence depends sensitively on the value of the EPC homing parameter γ This parameter was varied between and 1, and we found that G(t) becomes slower if γ is increased, and faster ... the interactions of maintenance factors such as cell division and stem cell homing, and damaging factors such as cellular senescence and death Our model involves a system of differential equations ... Results and Discussion), λi h YW performed the numerical simulations BDA and AF formulated the model equations, wrote the manuscript, and jointly supervised YW's work All authors read and approved...

Ngày tải lên: 13/08/2014, 16:21

... glycosylation, neddylation, sumolyation and ribosylation (Meek, 1999; Appella and Anderson, 2001; Bode and Dong, 2004; Toledo and Wahl, 2006; Olsson et al., 2007; Anderson and Appella, 2009) These posttranslational ... senescence 1.1.3.1 p16INK4A and Retinoblastoma 1.2 11 11 p53, the guardian of the genome 12 1.2.1 p53 Domains 13 1.2.2 p53 functions 14 1.2.2.1 p53 and Senescence 16 1.2.3 p53 regulation in senescence ... 1.3.2 Stra13 functions 24 1.3.2.1 Stra13 in cancer, apoptosis, cell cycle arrest and senescence 26 1.3.2.1.1 Stra13 in relation with p53 and senescence 1.4 27 Sirt1, a nicotinamide adenine dinucleotide...

Ngày tải lên: 10/09/2015, 09:23

... Apoptosis, Senescence, and Cancer Cancer Drug Discovery and Development Beverly A Teicher, Series Editor Apoptosis, Senescence, and Cancer, edited by David A Gewirtz, Shawn E Holt and Steven ... Cancer Drug Discovery and Development Apoptosis, Senescence, and Cancer Edited by: D A Gewirtz, S E Holt and S Grant © Humana Press Inc., Totowa, NJ Part I / Apoptosis and Alternative Modes of ... Cancer Drug Discovery and Development Apoptosis, Senescence, and Cancer Edited by: D A Gewirtz, S E Holt and S Grant © Humana Press Inc., Totowa, NJ 31 32 Part I / Apoptosis and Alternative Modes...

Ngày tải lên: 29/06/2014, 06:20

... and PARP in apoptosis, DNA repair, genomic stability and functions of p53 and E2F-1 Adv Enzyme Regul 2000;40:183-215 Wang H, Shimoji M, Yu SW, Dawson TM, Dawson VL Apoptosis inducing factor and ... interrupt replication and activate ATR-dependent DNA damage repair and apoptosis The pathway responsible for the repair of single-strand nicks in DNA is also important for NS1-induced apoptosis This ... in a covalent manner, and that both DNA-helix distorting and single strand nick forms of DNA damage are important pathways to apoptosis upon expression of NS1 Materials and Methods Transfection...

Ngày tải lên: 25/10/2012, 11:18

... 1,25-(OH)2D3 on podocyte apoptosis and podocytopenia The present study aimed to investigate the effects of 1,25-(OH)2D3 on podocyte apoptosis and podocytopenia induced by adriamycin (ADR) and the potential ... Bax/Bcl-2 ratio and finally limiting glomerular cell apoptosis Our study demonstrates the protective effect of 1,25-(OH)2D3 on podocyte apoptosis by down-regulating Fas, FADD and Bax and up-regulating ... collagenase and culture Early cellular outgrowths at 5-7 days were selectively removed by a cylinder cloning technique Cells were replated, and plates growing pure colonies were then expanded and identified...

Ngày tải lên: 25/10/2012, 11:40

... Ras and the telomerase catalytic subunit (hTERT) can transform HDFs and human keratinocytes (Hahn et al 1999) T-Ag inactivates the p53- and Rb-dependent senescence- inducing pathways and hTERT Senescence ... determination between senescence and immortalization These proteins bind to the E2F family transcription factors and maintain the repressor function of E2F (Hatakeyama and Weinberg 1995; Stevaux and Dyson ... predominantly upregulated during senescence by oncogene activation and/ or oxidative stress Senescence and Cell Cycle Control 265 In human cells undergoing replicative senescence, p16INK4a could be...

Ngày tải lên: 25/10/2013, 21:20

... and isoxanthohumol on 3T3-L1 cell apoptosis and adipogenesis Apoptosis 12, 1953–1963 15 Yang JY, Della-Fera MA & Baile CA (2008) Guggulsterone inhibits adipocyte differentiation and induces apoptosis ... 15-deoxy-D12,14-prostaglandin J2 (PGJ2), and reversed by GW9662, a PPARc antagonist Further analysis suggests that TZDs induce the downregulation of Akt-1 and lead to the activation of caspase-3-dependent apoptosis ... of GW9662 In addition, pioglitazone, another TZD, and PGJ2, which directly binds to and activates PPARc [29], also induce adipocyte apoptosis, and GW9662 treatment blocked this induction (Fig...

Ngày tải lên: 16/02/2014, 09:20

... resistance to apoptosis Apoptosis is a tightly regulated mechanism for the disposal of damaged cells and to remove cells during normal growth and development [27,40] Cells that undergo apoptosis ... with endosomal and Golgi structures using specific markers (EEA1, TfnR for endosomes and GM130, TGN46 for Golgi) and found that JSP1-wt partially colocalized with the Golgi apparatus and showed minimal ... dish and undergo apoptosis Interestingly, the cells could tolerate high levels of the myristoylationdeficient mutant and remain attached, whereas similar levels of the wild-type protein induced apoptosis...

Ngày tải lên: 16/02/2014, 14:20

... of TJ proteins such as claudin-1 and claudin-4 induces apoptosis and suppresses metastatic potencies [10,11] Occludin has also been reported to induce apoptosis and apoptotic sensitization, which ... occludin (OccWT) and variant occludin in apoptosis and invasion, as determined by assay, we revealed that exon played a major role in the induction of mitochondria-mediated apoptosis and the reduction ... concentration increases apoptosis and decreases invasiveness On the basis of the differences between OccWT and OccDE9 with regard to mitochondria-dependent apoptosis and reduction of invasion,...

Ngày tải lên: 18/02/2014, 18:20

... lane 6, Glu-Pg- and mAb 34D3-treated cells Bands in lanes and correspond to forms I and II of plasmin(ogen) High molecular weight bands in lanes and correspond to mAb A10.2- and mAb 34D3-plasminogen ... Lys-plasmin(ogen) binding and activation These structural–functional transitions are determinant in the initiation and development of both fibrinolysis and cell detachment-induced apoptosis and may therefore ... their respective contribution to Gluand Lys-Pg binding and activation onto fibrin and cells, as well as their effects on fibrinolysis and on plasmin-induced cell apoptosis The LBS specificity of these...

Ngày tải lên: 20/02/2014, 01:20

... (ATZ) and mercaptosuccinic acid inhibited catalase and glutathione peroxidase, which are antioxidative enzymes that eliminate hydrogen peroxide, and caused sustained increases in ROS levels and apoptosis ... lM) or vitamin C (1 mM), and then incubated for h in the presence or absence of ATZ (20 mM) and mercaptosuccinic acid (7 mM) Cell were collected and lysed, and both firefly and Renilla luciferase ... transactivation and apoptosis, supporting the hypothesis that c-Fos and c-Jun act coordinately to increase the expression of BimEL Increased c-Fos levels are therefore critical for BimEL expression and apoptosis...

Ngày tải lên: 06/03/2014, 00:21

... cells, and is implicated in the restoration of genomic integrity in response to DNA double-strand breaks [80] B23 negatively regulates p53 and antagonizes stress-induced apoptosis in human normal and ... the cytoskeleton in fibroblasts and delay the execution of apoptosis Cleavage of the p53–vimentin complex enhances TNF-a-related apoptosis- inducing ligand-mediated apoptosis in fibroblasts [50,52,53] ... Sprick MR, Weigand MA, Rieser E, Rauch CT, Juo P, Blenis J, Krammer PH & Walczak H (2000) FADD ⁄ MORT1 and caspase-8 are recruited to TRAIL receptors and and are essential for apoptosis mediated...

Ngày tải lên: 06/03/2014, 22:21

... 20 and 30 and overnight incubation (B) Lanes 2–8, proteolysis products of RhdA-PS (lane 1) at 0, 5, 10, 15, 20, 30, 60 and overnight incubation, respectively ‘a’ and ‘b’ bands are the parent and ... RhdA(ES), as shown in Fig 6B ,and a band rapid digestion was observed A stable daughter band (b band), of about 17.3 kDa, appeared after a few minutes of proteolysis and was present also after many ... bovine serum and antibiotics (1% penicillin and streptomycin sulfate) at 37 °C in air supplemented with 5% CO2 and were then exposed to 2-PTS for 24 and 48 h The cells were collected and counted,...

Ngày tải lên: 07/03/2014, 06:20

... indicative of an apoptosis ratio of 4.73% As the duration of treatment increased to 48 and 72 h, the ratio increased to 19.91% and 31.71% In contrast, apoptosis ratios were 4.01% and 4.09% when ... Ac-DEVDCHO and 28 lm compound 1, respectively Apoptosis ratios were 2.27% and 2.15%, respectively, when cells were treated with lm Ac-DEVD-CHO and 28 lm compound but not with Ab(25–35) The apoptosis ... isoquinoline-1,3,4-trione and its derivatives as selective, irreversible, slow-binding, pan-caspase inhibitors This compound and its derivatives protected PC12 cells and primary cortical neuronal cells against apoptosis...

Ngày tải lên: 07/03/2014, 11:20

... blue and red, respectively The BIR2 and BIR3 domains are shown in green The dashed yellow lines connect the ATIF peptides of each AFP monomer and the IBM-interacting grooves of the BIR2 and BIR3 ... cell lines and tissues has shown that many tumor cell lines and tissues have constitutively higher levels of active caspase and free cytochrome c in the absence of apoptotic stimuli and yet are ... equilibrium between pro- and antiapoptotic signals Taking into account that normal cells and tissues not overexpress apoptotic stimuli and IAPs, whereas cancer cells and tissues do, IAP-targeting...

Ngày tải lên: 07/03/2014, 12:20

... microvilli and junctional complexes, and blebbing can occur In several cases, an intense endocytosis is observed, and this probably Fig Multiple pathways to apoptosis, both dependent and independent ... activation (GluRd2-Lc) Yue and collaborators [113] showed that GluRd2, nPIST and beclin-1 interact, and that autophagy can be induced by nPIST and beclin-1 synergy and by the mutated GluRd2-Lc, ... section will examine its relationship with apoptosis Apoptosis vs autophagy Notwithstanding the abundant evidence for a role for both autophagy and apoptosis in various diseases, their interplay...

Ngày tải lên: 07/03/2014, 15:20