apoptosis and alternative modes of cell death

... xv Part I: Apoptosis and Alternative Modes of Cell Death The Intrinsic Pathway of Apoptosis Scott H Kaufmann The Extrinsic Pathway of Apoptosis 31 M Stacey Ricci, and Wafik S ... CD95/CD95 ligand system is not the major effector in anticancer drug-mediated apoptosis Cell Death Differ 1998; 5:735–42 28 Part I / Apoptosis and Alternative Modes of Cell Death 208 Landowski TH, ... mechanism of IAPs EMBO J 2005; 24(3):645–55 26 Part I / Apoptosis and Alternative Modes of Cell Death 160 Miller LK An exegesis of IAPs: salvation and surprises from BIR motifs Trends Cell Biol...

Ngày tải lên: 29/06/2014, 06:20

... no tumor cells exhibiting IR; 1, < 25% of the tumor cells exhibiting IR; 2, 25–50% of the tumor cells exhibiting IR; 3, 50–75% of the tumor cells exhibiting IR; 4, > 75% of the tumor cells exhibiting ... expression of Akt and PrPC was examined by immunohistochemistry and confocal microscope in human gastric cancer cases and cell lines Expression of total and p-Akt was detected in cells transfected ... intensity of intracellular adriamycin was determined using flow cytometry with an excitation wavelength of 488 nm and an emission wavelength of 575 nm (A) Fluorescence intensity analysis of intracellular...

Ngày tải lên: 07/03/2014, 03:20

... cells We sought to identify the targets of a range of NF-κB and CDK inhibitors in HTLV-1 infected and uninfected cells by culturing MT-2, MT-4, C8166, c10/MJ and uninfected CEM and Jurkat T-cells ... activity in vitro and induced higher level of apoptosis in infected cells Finally, the efficacy of combination of both BMS-345541 and Purvalanol A in inhibiting HTLV-1 infected cells was tested ... [43,44] and by bortezomib, a proteasome inhibitor [45], induced apoptosis of HTLV-I-infected T-cells and ATL cells, suggesting that inhibitors of NF-κB may be effective targets against ATL cells...

Ngày tải lên: 10/08/2014, 05:21

... S6 and the GST-tag (C) Immunoblot analysis of the phosphorylation status of p70S6K and S6 in Paskin+ ⁄ + and Paskin) ⁄ ) MEFs (D) Immunoblot analysis of the phosphorylation status of p70S6K and ... phosphorylation is a determinant of cell size and glucose homeostasis Genes Dev 19, 2199–2211 28 Meyuhas O (2008) Physiological roles of ribosomal protein S6: one of its kind Int Rev Cell Mol Biol 268, 1–37 ... purified from SF9 insect cells was used for in vitro phosphorylation of a microarray of 1176 peptides in the presence of [c-33P]ATP The magnified inset shows an example of the results obtained...

Ngày tải lên: 06/03/2014, 00:21

... uptake of Ni nanoparticles during the process of endocytosis that led to the change of the cell membrane and formation of holes (arrows) on the surface of the relevant cell into cancer cells and ... lmol/L of quercetin solution and cells incubated for h; d 50 lmol/L of quercetin solution and cells exposed to Ni nanoparticles cocultured for h and e 50 lmol/L of quercetin solution and cells ... effect of Ni nanoparticles can efficiently produce some holes and/ or result in the release of the cytosol out of the cancer cell and thus make the cell death, whereas at the lower concentrations of...

Ngày tải lên: 22/06/2014, 00:20

... (0.54 and 2.16 mg/ml, respectively) glucosamine (Sigma) and then stimulated with 10 ng/ml IL-1β for 22 hours Culture supernatants were collected and analyzed by ELISA, and cells were harvested and ... Effect of glucosamine on MMP-1 and MMP-13 expression in lbpva55 cells stimulated with 10 ng/ml IL-1β Cells were pretreated for hours with 2.5 on MMP-1 and MMP-13 expression in lbpva55 cells stimulated ... IL-1β-induced phosphorylation of JNK, p38 and ERK1/2 MAP kinases in lbpva55 cell line and HPCs We analyzed the phosphorylation levels of three MAPKs, namely JNK, p38 and ERK1/2, by Western blotting...

Ngày tải lên: 09/08/2014, 10:21

... endothelial cell plays a crucial role in angiogenesis [39] and that it might affect the growth and differentiation of neighboring cells [40] The endothelial cell is also the target cell for tumor ... and GAPDH and PCR conditions were as previously described [20] Analysis of c-myc gene transcription by nuclear runoff assay To prepare ECV304 nuclei, cells were washed with ice-cold NaCl/Pi and ... Briefly, 90 lL of nuclei suspension were added with 100 lL of · reaction buffer (10 mM Tris/HCl, pH 7.5, mM MgCl2, 0.3M KCl, mM dithiothreitol, mM each of ATP, GTP, and CTP), and lL of [a-32P]UTP...

Ngày tải lên: 21/02/2014, 01:21

... proliferation of leukemic cells in AML and inhibition of activation of Akt can result in suppression of cell growth [40,41] In the present study, phosphorylation of Akt-1 and GSK3b, the downstream of Akt-1, ... equipped with CellQuest software (BD) Identification and quantification of apoptotic cells with Hoechst 33342 Nuclear morphology of control and VX-680 treated cells was observed by staining cell nuclei ... Apoptosis of NB4-R2 cells(%) B 80 *** 70 60 50 *** 40 *** *** 24h 30 48h ** 20 10 0 10 VX-680(nM) Figure VX-680 suppresses the proliferation of NB4-R2 cells and induces cell apoptosis NB4-R2 cells...

Ngày tải lên: 18/06/2014, 19:20

... inhibition of these pathways in regard to the expression of cyclooxygenase (COX)-2 and microsomal prostaglandin E synthase-1 (mPGES-1) and to the production of prostaglandin (PG) E2 and PGD2 Findings ... inhibition of GSK-3 reduced the expression of both COX-2 and mPGES-1 In contrast, the mTOR inhibitor rapamycin enhanced both COX-2 and mPGES-1 immunoreactivity and the release of PGE2 and PGD2 ... increases the production of prostaglandins It has been shown that mTOR inhibitors reduced NOS activity and iNOS expression induced by hypoxia and a mixture of cytokines in BV-2 cells and primary rat microglia,...

Ngày tải lên: 19/06/2014, 22:20

... solution, 0.01 g of TPTC powder was dissolved in 10 ml DMSO and then diluted to a final concentration of 1000 ppm Cell toxicity assay of TPTC The effect of TPTC on the survival of WB F344 cells was ... Journal of Occupational Medicine and Toxicology 2010, 5:17 http://www.occup-med.com/content/5/1/17 Page of 10 Figure Cytotoxicity of TPTC in WB-F344 liver cell (A) Cell viability of WB-F344 liver cells ... P0 band disappeared, and a shift to bands of higher molecular weight occurred (P1) (Fig 6) Effects of TPTC on connexin 43 in immunofluorescence staining The expression of Cx43 in WB-F344 cell...

Ngày tải lên: 20/06/2014, 00:20

... proliferation of leukemic cells in AML and inhibition of activation of Akt can result in suppression of cell growth [40,41] In the present study, phosphorylation of Akt-1 and GSK3b, the downstream of Akt-1, ... equipped with CellQuest software (BD) Identification and quantification of apoptotic cells with Hoechst 33342 Nuclear morphology of control and VX-680 treated cells was observed by staining cell nuclei ... Apoptosis of NB4-R2 cells(%) B 80 *** 70 60 50 *** 40 *** *** 24h 30 48h ** 20 10 0 10 VX-680(nM) Figure VX-680 suppresses the proliferation of NB4-R2 cells and induces cell apoptosis NB4-R2 cells...

Ngày tải lên: 20/06/2014, 03:20

... presence of IL-10, the induction of STAT1 and STAT3 phosphorylation by IL-10, and the expression of SOCS1 and SOCS3 mRNA in peripheral blood (PB) CD4+ T cells from RA patients Materials and methods ... in sustaining the activation of macrophages and synovial fibroblasts through cell surface signaling by means of cell surface CD69 and CD11, as well as the release of proinflammatory Th1 R568 cytokines ... treatment of active disease by selective inhibition of T -cell activation with fusion protein of cytotoxic T -cell- associated antigen (CD152)-IgG, which can block the engagement of CD28 on T cells...

Ngày tải lên: 09/08/2014, 01:24

... undertaken, and the research protocol was in accordance with the institutional guidelines of the Animal Care and Use Committee HeLa-pcDNA and HeLa-DPK3-scFv cells of 10 cells resuspended in 200 μl of ... therapeutic agent and the mechanistic study of DNA-PKcs in the cellular responses to radiation Methods Cell culture HeLa-DPK3-scFv and HeLa-pcDNA cell lines were generated from HeLa cells by transfecting ... hybridoma cell line expressing the mAb 18-2 antibody of DNA-PKcs However, it is necessary to expand this kind of study, especially to verify the efficacy and mechanisms of the radiosensitization of...

Ngày tải lên: 09/08/2014, 09:20

... phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater ... mechanism of pancreatic islets FN and LAM enhances activation of Akt and ERK The ECM bind to cells by activating signaling molecules such as Akt and ERK Therefore, we analyzed the effect of FN and ... other hand, PD98059 decreased the activation of ERK both in cells treated with FN or LAM (Figure 5B and 5D) and increased the activation of Akt about 2.7-fold and 2.1fold compared to FN and LAM...

Ngày tải lên: 10/08/2014, 05:21

... Page of Figure Knockdown of Rab5a decreases SiHa cell motility and invasion (A) In Control and Rab5a-siRNAs cells, the expression of Rab5a was confirmed by Western blot (B) The number of cells ... percentage of cells showing positive immunoreactivity (0, no staining; 1, 50% of cells), and the intensity of staining (graded 0, negative; 1, weak; 2, moderate; and 3, strong) ... metastasis of cancer cells Migratory cancer cells undergo series of morphological changes by the reconstruction of adhesion and actin cytoskeleton It is now widely accepted that the process of cancer cells...

Ngày tải lên: 10/08/2014, 10:20

... Effects of Cpc on cell viability tyrosinase activity, and melanin production Figure 1A shows the viability of B16F10 melanoma cells after treating with Cpc The viability of melanoma cells was ... antimelanogenesis in B16F10 melanoma cells Effects of Cpc on the up-regulation of MAPK/ERK pathway and the down-regulation of MITF Cellular localization analysis The Cpc-induced responses of MAPK/ERK pathwayassociated ... inhibitor of ATP binding of MAP kinase Wu et al Journal of Biomedical Science 2011, 18:74 http://www.jbiomedsci.com/content/18/1/74 Page of 11 Figure The entry of Cpc into B16F10 melanoma cells Cells...

Ngày tải lên: 10/08/2014, 10:20

... Page of Figure Inhibition of statin-induced apoptosis in C6 glioma cells by intermediates of the mevalonate pathway (A) Induction of caspase-3-like activity associated with statin-induced cell death ... determined the cell survival rate, which was defined as the ratio of the number of living cells after 24, 48, and 72 h of incubation with 1, 2.5, 5, 10 μM mevastatin, 1, 2.5, 5, and 10 μM fluvastatin ... number of U251MG cells significantly decreased at 72 h after the administration of 10 and 20 μM simvastatin Statins-mediated activation of caspase-3 The cytotoxic effects of statins on C6 glioma cells...

Ngày tải lên: 10/08/2014, 10:21

... emergence of order…[allowed by] a constant flow of energy and matter through the system…[the] emergence of new structures and new forms of behavior…[which are] the hallmark of self-organization, [and] ... illnesses such as cancer, heart disease and various kinds of inflammation… [and that] the capacity of stem cells to develop into different types of specific cells with well-defined functions in different ... point of oscillations which is characterized by a pre-set number of cell cycle repetitions and by some specificity of functioning Progenitor cells may serve as such an example The development of...

Ngày tải lên: 26/10/2012, 10:04

... the type of transference which has taken place The eye of a man - The eye of a potato The bowel of a person - The bowel of the earth The hand of a child - The hand of a lock The heart of a man ... percentage of the forms of each types of semantic transference and the percentage of the types of semantic transference used in these classes Table 2: The percentage of the forms of each type of semantic ... war The arm of a person - The arm of jacket 10 One's breast - The chest 11 One's foot - The foot of sock 12 One's hands - The hands of a farm 13 The heel of a person - The heel of the hand 14 One's...

Ngày tải lên: 18/12/2013, 21:45

... concentrated and used for biochemical analysis SDS/PAGE of purified BHs Fig Effect of camalexin and 3-phenylindole on the activity of BH from mycelial cultures of Leptosphaeria maculans (A) and Alternaria ... Table and Fig 4, the Hill equation provided an excellent fit, with coefficients of determination of 0.992 for BHLmL2 and 0.997 for BHAb In addition, the fits of the V = f(S) curves were sigmoidal and ... 85% inhibition of BHLmL2 and BHAb, respectively, while mm 2-mer- captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively The influence of pH on the activities of the BH enzymes...

Ngày tải lên: 18/02/2014, 14:20