antitumoral potential of the streptococcal toxin streptolysin s

... were explored instead of a continuous range of pH values; the latter is indispensable for monitoring all of the successive steps and structural transitions of the toxin domains leading to membrane ... closed red triangles) These two successive transitions have been observed previously [3] The increase of the kmax observed during the first transition could be attributed to increased exposure of ... of this chaperone activity is that it stabilizes at least three different partially folded states of the C domain, each corresponding to one of the successive steps of the initiation of translocation:...

Ngày tải lên: 14/03/2014, 22:20

... superimposed Consistent with the CD spectra, the amide resonances of most of the residues in secondary structural elements were unperturbed; however, the Y190A mutation caused nearly all of the ... addition, the impact of the most disruptive mutant, Tyr to Ala, at this position on the active site structure was characterized by NMR spectroscopy Results CD spectroscopy and HSQC analyses of 15 ... elucidation of the catalytic mechanism of these enzymes would allow a more comprehensive understanding of the origin of substrate specicity and structure function relationships Previously, studies of initial...

Ngày tải lên: 16/03/2014, 00:20

... drugs could be used to help resolve AIDS-related infections, as well as serve as a new class of antibiotics – virally derived antibiotic peptides Methods Peptide Synthesis Peptides were synthesized ... Anti-Retroviral Therapy (HAART), which combines the use of protease inhibitors and reverse transcriptase inhibitors, and use of the newer fusion inhibitors such as T20, HIV continues to be a serious threat ... bilayers, and disrupt lipid membranes Since Env is known to associate in trimers on the cell surface and in virions [42,43], it is easy to speculate that the LLP regions of the Env trimers could associate...

Ngày tải lên: 18/06/2014, 18:20

... assess the relationship between ordinal data The two-tailed P value was considered significant when ≤ 0.05 SPSS software (version 10.00, SPSS, Chicago, IL, USA) was used for statistical analysis ... of the muscle fibers, and fibrosis (Figure 2) Since we inserted the needle within the posterior muscles of the thigh, a more severe damage was observed at the insertion points Similar results ... years, since the first phase III EP trial was performed [24] In these cohorts of patients different voltages, waveforms and delivery modes (i.e single pulses versus bursts) were tested [24-35] The...

Ngày tải lên: 18/06/2014, 22:20

... drugs could be used to help resolve AIDS-related infections, as well as serve as a new class of antibiotics – virally derived antibiotic peptides Methods Peptide Synthesis Peptides were synthesized ... Anti-Retroviral Therapy (HAART), which combines the use of protease inhibitors and reverse transcriptase inhibitors, and use of the newer fusion inhibitors such as T20, HIV continues to be a serious threat ... bilayers, and disrupt lipid membranes Since Env is known to associate in trimers on the cell surface and in virions [42,43], it is easy to speculate that the LLP regions of the Env trimers could associate...

Ngày tải lên: 20/06/2014, 01:20

... assess the relationship between ordinal data The two-tailed P value was considered significant when ≤ 0.05 SPSS software (version 10.00, SPSS, Chicago, IL, USA) was used for statistical analysis ... of the muscle fibers, and fibrosis (Figure 2) Since we inserted the needle within the posterior muscles of the thigh, a more severe damage was observed at the insertion points Similar results ... years, since the first phase III EP trial was performed [24] In these cohorts of patients different voltages, waveforms and delivery modes (i.e single pulses versus bursts) were tested [24-35] The...

Ngày tải lên: 20/06/2014, 04:20

... total of eight Norway spruces (Picea abies, Karst.) and eight Scots pines (Pinus sylvestris, L.) in two test regions in Saxony was selected as sample material for the development of the classification ... pure Scots pine stands The aim of the subproject was among others to analyse, how conversion influences the percentage of merchantable assortments in the growing stock with special consideration of ... transfer of the results and (2) of a later re-investigation of the sample trees for value increment monitoring supports thus the differentiated analysis of stem surface structures and allows in...

Ngày tải lên: 07/08/2014, 16:20

... approaches described above We also assessed the plasticity of thermostability by measuring seasonal changes of Tc in the four species, and by submitting the potted trees to increasing temperatures in ... chambers using stepwise increases of temperature (5 days steps) imposed to whole seedlings During the two experiments, Tc increased in all species (p < 0.0001) in response to the stepwise increase of ... [6] Heteromeles arbutifolia 48.5 [39] Pinus halepensis Quercus ilex Quercus suber Pinus halepensis Pinus nigra Cupressus sempervirens Cedrus atlantica S 30 °C Annuals S 25 °C Species S 20 °C Table...

Ngày tải lên: 08/08/2014, 01:22

... 264 isoforms of the unknown dataset Probe set expression levels were computed as the median of normalized expression values in the 11 tissues in the panel Isoform expression is estimated as the ... sites present in some other isoform of the same gene); false positive (FP) - an unknown or negative set isoform for which the considered descriptor is consistent with the hypothesis of the isoform ... an assessment of the plausibility of the modeled structure of the isoform increases the coverage by another 8%, with a decrease in sensitivity, but in this case the lower estimate for the specificity...

Ngày tải lên: 09/08/2014, 22:23

... potential statistics for an alignment of four plant fijivirus segment sequences Coding potential statistics for an alignment of four plant fijivirus segment sequences (1)-(3) The positions of stop ... synonymous sites in ORF5-1 Beginning with pairwise sequence comparisons, conservation at synonymous sites (only) was evaluated by comparing the observed number of base substitutions with the number ... the same frame as the other sequences via the arbitrary insertion of an extra 'U' into the run of five 'U 's at NC_007160 nucleotides 2714–2718, as discussed in the text Note the conserved absence...

Ngày tải lên: 12/08/2014, 04:21

... the solution located at the right side, is a proof of compound diffusion from the donor liposomes, passing across the dialysis membrane and incorporation in the membrane of the acceptor liposomes ... Discovery Screen,” using the protein-binding dye sulforhodamine B to assess cell growth [15,16] Doxorubicin was tested following the same protocol and was used as positive control Results and discussion ... PMF supervised the organic synthesis and compound characterization and participated in the draft of the manuscript LAVS was responsible for the antitumoral evaluation of the compound EP supervised...

Ngày tải lên: 21/06/2014, 01:20

... on musical subjects using high ISIs, a clear link between musicality and larger MMN amplitudes suggests that musical subjects possess enhanced auditory sensory memories as compared to nonmusical ... levels (i.e., at lower amplitudes) with missed targets and standard stimulus presentations This suggests the contingency of the N2b amplitude not upon the recognition of the actual deviant targets ... observed in tasks which require the subject to respond to the rare stimulus with button-presses In visual oddball tasks, observed active and passive latencies for the P300 are comparable, suggesting...

Ngày tải lên: 02/11/2012, 11:08

... high cost of implementing such plants is compensated for by the reduction in the pathogen levels as the sludge stays in the digester longer The business-as-usual scenario at the KNUST sewage ... 100 The composition of biogas largely depends on the type of substrate Human excreta based biogas contains 65-66% CH4, 32-34% CO2 by volume and the rest is H 2S and other gases in traces whiles the ... university Figure Feeds into the sewage treatment plant After the sewage enters the treatment plant, it goes through various treatment processes discussed in subsection The flow of sewage to the...

Ngày tải lên: 05/09/2013, 16:11

... Biofumigants for the Control of Pest Infestation 395 sativa, Sisymbrium irio, Sinapis alba, S arvensis, E sativa, and Diplotaxis spp., only in the last three species was it possible to isolate from the ... hydrolysis of sinigrin to ITC by the enzyme myrosinase which is found inside the seeds The second step is steam distillation with use of the Dean–Stark apparatus (Leoni et al., 1997) The yellow ... Analysis, sorption and residues of a homologous series of isothiocyanates on wheat Pestic Sci 44: 249–253 Shaaya, E., Kostyukovsky, M 2006 Essential oils: potency against stored product insects...

Ngày tải lên: 25/10/2013, 05:20

... progress as a result of these decisions The state of Texas has sued CMS to prevent the loss of federal funds and a group of Planned Parenthood clinics has sued the state to prevent implementation of ... rise several-fold Paradoxically, as the number of WHP patients who can continue to be served climbs, the state s budget losses grow larger These are estimates based on some basic assumptions; the ... affiliate has a billing address in Dallas County, but some of its sites are in other adjacent counties and some of the patients served may be residents of other counties The list does not indicate...

Ngày tải lên: 13/02/2014, 06:20

... was gained from studies with synthetic iron complexes assumed to be model systems for BLM because of their capabilities to inict DNA strand scission in the presence of ascorbate/O2 [192,243] The ... scale of the measurements, of hydroperoxo-iron component relative to the standard redox situation (Scheme 2) This is likely to cause an increase in both the half-life of the scission process and the ... analysis of P450 function Steroidogenic P45 0s belong to the category of isozymes promoting multifunctional biosynthesis of endogenous compounds Thus, 17a-hydroxylase-17,20-lyase (CYP17) sustains...

Ngày tải lên: 19/02/2014, 16:20

... different sizes of the substituents In the case of TpT, intramolecular stacking of thymine residues brings the two C(5)ẳC(6) bonds in close proximity, thereby increasing the probability of their ... segments of the r70 and b, b RNA polymerase subunits The molecular mechanism of DNA strand separation is still unknown, however Studies with mutant polymerases harboring deletions in these subunits ... and absence of Mg2+ This conclusion also applies to RPo at kPR and other promoters Discussion The results show that (a) all nine thymine residues of the template and nontemplate strands in the...

Ngày tải lên: 19/02/2014, 18:20

... equilibrium shifts toward the free version of r32, leading to induction of the stress response Nevertheless, the nature and variability of the recombinant protein stress response suggests a far more ... aggregates is often limited by the intrinsic insolubility of the samples, responsible for a high level of light scattering disturbances and signal loss The characteristic presence of b-sheet structures ... mutations and overexpression, as discussed in the next paragraphs Also, ‘quality’ of protein synthesis is affected by environmental stress due to an increase in the rate of translational errors, amino...

Ngày tải lên: 06/03/2014, 00:20

... exercise is an assessment of the current market size and future market potential of various branches of the virtual economy There are two basic approaches to assessing the size of an industry ... each others’ virtual possessions As a result, virtual goods in the game obtain a social status value in the same way as consumer goods in physical environments Some players would rather buy those ... services served by gaming studios This is followed by an analysis of the size of the gaming studio market The analysis includes an overview of earlier estimates from the literature as well as...

Ngày tải lên: 06/03/2014, 10:20

... Foundation of Agricultural Economics Timothy J Richards is Power Professor of Agribusiness, Morrison School of Agribusiness, Arizona State University Paul M Patterson is Associate Professor, Morrison School ... cost of re-shelving, or simply to share the cost of promotion, the imposition of such charges nonetheless raises several economic and legal issues, especially when shippers realize few of the shared ... offering these fees and allowances In some cases, a supplier may feel that its rivals use these fees to block their access to retail shelves This argument, the “Essential Facility Doctrine,” is supported...

Ngày tải lên: 07/03/2014, 13:20