antisera western blot expression analysis and quantification of particle release
Báo cáo y học: "Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells" potx
... effect of the mutation was also investigated on the structure of the viral particle by electron microscopy analysis, and normal-sized mature virions were found in preparations of WT and ASPmut12 particles ... its detection through Western blot analysis from cellular extracts has not yet been possible Different reasons can explain the lack of detection of the ASP protein First of all, antisense retroviral ... by ELISA at days 3, 7, 10 and 14, and represent the mean values +/- S.D of three independently infected samples Western blot Transfected 293T cells were washed in PBS and proteins were isolated...
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Báo cáo y học: "Biochip sensors for the rapid and sensitive detection of viral disease" pps
... been greatly expanded to include the production of all manner of arrayed probes: cells, glycans and carbohydrates, to name but a few The significance of the microarray to the field of infectious ... viral analysis can be divided into viral chips and host chips, and each can be applied not only to detection and identification but also to the monitoring of viral populations In 1999, we and colleagues ... Sample preparation and hybridization DNA array Scanning of array Protein array Image analysis Figure The structure of microarray experiments (a) To obtain gene -expression profile data from a cDNA...
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Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
... activity in both human and mouse skin [16,19] These studies have provided new insight into the substrate specificity of Tgases and have expanded the range of application of the enzyme reaction ... mono-avidin gel (SoftLinkÔ Soft Release Avidin Resin; Promega Corp., Madison, WI, USA) After washing with TBS containing 0.1 or 0.5% Tween 20 and mm EDTA and then with TBS, the bound phage particles ... The concentration of the purified protein was determined by quantification of the intensity for the separated bands in SDS ⁄ PAGE analysis using imaging software (multigauge software; Fujifilm,...
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Báo cáo sinh học: " Biochemical prevention and treatment of viral infections – A new paradigm in medicine for infectious diseases" doc
... or RNAi, is the inhibition of expression of specific genes by double-stranded RNAs (dsRNAs) It is becoming the method of choice to knockdown gene expression rapidly and robustly in mammalian cells ... to reduce the rate of hospitalization of at-risk infants by about 55% in clinical studies and now serves as the primary medical means of RSV prevention [11-13] Prevention of Human Rhinovirus ... Targeting viral mRNA is one of the most active areas of research and development Several strategies have emerged over the years and are being tested pre-clinically and clinically They include:...
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Báo cáo sinh học: " CODEHOP-mediated PCR – A powerful technique for the identification and characterization of viral genomes" pptx
... genomes Often the linear order of genes within the genomes of related viruses is maintained Thus, the spacing and orientation of specific genes can be predicted in the genomes of Page 15 of 24 ... phylogenies and tracing of evolutionary pathways Furthermore, comparison of multiple representatives of homologous viral proteins can be of importance for understanding the protein structure and function ... presented (in bold and boxed) and the other amino acids found at each position are aligned vertically above the consensus amino acid The sequences of the coding strand and complementary strand corresponding...
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báo cáo hóa học:" Biochemical prevention and treatment of viral infections – A new paradigm in medicine for infectious diseases" pdf
... or RNAi, is the inhibition of expression of specific genes by double-stranded RNAs (dsRNAs) It is becoming the method of choice to knockdown gene expression rapidly and robustly in mammalian cells ... to reduce the rate of hospitalization of at-risk infants by about 55% in clinical studies and now serves as the primary medical means of RSV prevention [11-13] Prevention of Human Rhinovirus ... Targeting viral mRNA is one of the most active areas of research and development Several strategies have emerged over the years and are being tested pre-clinically and clinically They include:...
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báo cáo khoa học: " Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen" pot
... YHTK YHTK YYTK YHTK Analysis of Bet v isoforms by Q-TOF LC-MSE The tryptic digests of the 1618 kDa bands were examined in detail to elucidate the expression of separate Bet v isoforms in pollen ... for isoform 02C01 and comprises 17% of all fragment IIIvariants, while peptides IIIb and Xb are unique for 01B01 and comprise 1819% of all fragment III and X-variants The isoforms 02A01 and 02B01 ... presence and relative abundances of individual isoforms The pollen of four birch species contained a mixture of Bet v isoforms, with abundant levels of isoforms that were similar to isoforms with...
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Báo cáo y học: "Eukaryotic large nucleo-cytoplasmic DNA viruses: Clusters of orthologous genes and reconstruction of viral genome evolution" pps
... platform for the functional and evolutionary analysis of new NCLDV genomes We also report some patterns of evolution of the NCLDV that can be inferred from a preliminary analysis of the NCVOGs http://www.virologyj.com/content/6/1/223 ... compendium of the core NCLDV functions Phylogenies of the core proteins of the NCLDV As the number of genomes of cellular life available for comparative analysis increases, the set of universal ... of genes of cellular organisms, in all likelihood, were acquired in the course of evolution of the mimivirus lineage, probably, from a variety of distinct cellular sources; the same process of...
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Báo cáo y học: " Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies" potx
... http://www.retrovirology.com/content/7/1/68 Diagnostics), and expressed in XL1-Blue cells and DH5a cells, respectively p15 (aa 1-129 of MA), p12 (aa 1-84 of p12), and p10, (aa 1-56 of NC) were cloned into pJO200 and expressed in XL1-Blue ... Diagnostics and the NCRR grant to the Yerkes National Primate Center DRR000165 and to RHS, grants from NIH (NCI) CA103943, US Department of Defense W81XWH-07-1-338; and Mal and Lea Bank and (to RHS and ... HIV and HTLV are also primarily to the envelope proteins (HIV-gp120, HTLV-gp46), transmembrane proteins (HIV-gp41, HTLV-gp21), and core proteins of capsid and matrix (HIV-p24 and p17, HTLVp24 and...
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Báo cáo y học: "Oral keratinocytes support non-replicative infection and transfer of harbored HIV-1 to permissive cells" ppsx
... min, 95°C, min, and 95°C, 15s and 60°C, 30s, for 50 cycles 50°C, min, 95°C, min, and 95°C, 15s and 60°C, 30s, for 50 cycles 95°C, and 95°C, s, 54°C, 10 s, 72°C, s for 40 cycles 95°C, and 95°C, 10 ... surface-bound particles Both surface-bound and internalized particles are infectious and effectively trans infect CD4+ cells (Fig 6) Cell-associated particles effectively trans infect PBMCs and MOLT-4 ... Use of surgical waste TE cells in research was reviewed and approved by the Research and Development Committee of the Minneapolis VA Medical Center and the Human Subjects Research Committee of...
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Báo cáo y học: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model" pptx
... Page of 16 (page number not for citation purposes) Retrovirology 2008, 5:90 http://www.retrovirology.com/content/5/1/90 Expression of SCTs of RT1.Al fused with EGFP Figure Expression of SCTs of ... BamH1 and Bsp1407I sites at the 5' and 3' end of the fusion constructs, respectively and were ligated between BamH1 and Bsp1407I sites of pEFGFP vector [27] In this study, we constructed expression ... epitopes and different lengths of linkers The diagram of SCT expression vectors established in this study was shown in Figure 1B The short or long linkers consist of 10 or 15 residues of L1 and 15...
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Báo cáo y học: "Common principles and intermediates of viral protein-mediated fusion: the HIV-1 paradigm" pps
... model of lipid bilayer fusion The stalk-pore model of lipid bilayer fusion (A) and consensus models for class I and class II protein-mediated membrane fusion (B and C) SU and TM are the surface and ... HIV-1 and Avian Sarcoma and Leukosis Virus (ASLV) particles and target cells [48,49] Model systems for studying viral fusion Entry pathways and modes of activation While the structures of ectodomains ... activities of fusion peptide mutants of influenza virus hemagglutinin J Virol 1995, 69:6643-6651 Hernandez LD, White JM: Mutational Analysis of the Candidate Internal Fusion Peptide of the Avian...
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Báo cáo y học: " PRMT6 diminishes HIV-1 Rev binding to and export of viral RNA" pps
... methylation of Rev by PRMT6 in vitro A, Sequences of recombinant histidine-tagged Tat86 and Rev Both sequences are chimeric and consist of BH10 (amino acids 2–66 and 2–15, respectively) and HXB2 ... 6% of control and R35K was not detectable The R39A and R39K substitutions resulted in band intensities that were either undetectable or 3% of wild-type, respectively Methylation of the R38A and ... methylation of HIV-1 Rev by PRMT6 represents a type of host defense mechanism that can limit rates of viral replication? Reduced binding of Rev to RRE and diminished export rates of unspliced and partially...
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Báo cáo sinh học: "Analysis of a simulated microarray dataset: Comparison of methods for data normalisation and detection of differential expression (Open Access publication)" pps
... simulation software Array number of grid rows Array number of grid columns Number of spots in a grid row Number of spots in a grid column Number of spot pins Number of technical replicates Number of genes ... those methods Data normalisation of gene expression analysis 677 Table III Summary of the results of the analysis of the simulated data set Table shows the number of genes identified by each method ... correct, the number of false positives and negatives, the number of correctly identified genes as a % of the total number of differentially expressed genes (624) and as a % of the number of genes identified...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... Temperature 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration ... recovery of soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that...
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Analysis and detection of human motion in time frequency domain
... decomposition and reconstruction process, we still need to understand the nature of the frequency bandwidth of decomposed and reconstructed signals Table 3.1 shows the frequency bandwidths of decomposed and ... mean and one side standard deviation of positive peak 122-124 and negative peak angular rates of gyroscope sensors at position FW, S and RU Both positive and negative peak angular rates of fall ... coefficients and 93 separated segments of human motion patterns in 12 the acceleration signals 4.20 44 pairs of Pa and Pv for ascending and descending stairs 96 and 66 pairs of Pa and Pv for level...
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Analysis and detection of human emotion and stress from speech signals
... fi center frequency of i subband bi bandwidth of i subband α logarithmic growth factor C bandwidth of first filter lm lower edge of m th filter bank hm upper edge of m th filter bank St ( m ) ... suggestion of universal facial expression and analyse the appropriateness of emotion display rules The display rules vary based on cultures And the influences of cultures on ways of behaving, modes of ... variety of other functions such as word and sentence stress or syntactic segmentation [27] The above studies concern the analysis of characteristics of emotional expressions and classification of...
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Application of support vector machine to prediction of cross reactive allergens and recognition of viral sequences
... receptors on mast cells and basophils This triggers effector cell degranulation and rapid release of stored mediators of allergic reactions (e.g histamines), and secretion of cytokines (Galli et ... CF of a codon is the total frequency of the codon over the total frequency of all codons (Eq 5) The term codon usage is of biological significance only to codons in coding genes and thus of little ... viruses, it is of particular interest to analyze these unknown sequences The methodic extension of a large number of such sequences by design of specific primers and sequencing is costly and time consuming...
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Comparative studies on the infection and colonization of maize leaves by fusarium graminearum, f proliferatum and f verticillioides
... moniliforme and F. subglutinans in 1983. In AustraliaandintheUnitedStates,F.graminearumwascapableofcausingheadblightof wheat,crownrotofwheatand stalkrotofmaize.F.culmorumfromfootrotofwheat and barley ... old stems andoncobsofmaize.Thestrawanddebrisofwheat,barleyandothercerealarethe mainreservoirofF.graminearum.Chlamydosporesorperitheciaofthisfungusformed on debris can survive over winter and infect ... preciseandreproduciblequantitationofDNAproducts.Typicalapplicationofrealtime PCR includes pathogen detection, gene expression analysis, single nucleotide polymorphism analysis, analysis of chromosome...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25