antioxidant activity and antigenotoxicity of cassia tora

... fragility and oxidative damage of erythrocytes of zinc-deficient rats J Nutr 1997; 127: 1290–1296 Lajos N, Miki N, Sandor S Protein and non-protein sulfhydryls and disulfides in gastric mucosa and ... spectrophotometric method [20] A mixture of 200 μL of 8% sodium dodecyl sulfate, 200 μL of 0.9% thiobarbituric acid and 1.5 ml 20% acetic acid was prepared 200 μL of RBCs lysate and 1.9 ml distilled water ... oxidative modification of erythrocytes [10] Antioxidants can help in preservation of an adequate antioxidant status; therefore, they preserve the normal physiological function of living cells by...

Ngày tải lên: 25/10/2012, 11:40

Ngày tải lên: 28/06/2014, 11:20

... content of GSH (only one-half of that present in A549) and poor GPx activity (Figs and 2); however, RD cells differ significantly in GR activity and in particular in activity, gene expression and ... obtained SOD activity was expressed as unitsÆmg protein)1, with unit of SOD activity being defined as the amount of proteins causing half-maximal inhibition of the rate of NBT reduction GST activity ... mM NADPH, and % 300 lg protein The specific activity was calculated by using an extinction molar coefficient obtained by a standard curve of NADPH between 0.02 and 0.1 lmolesÆmL)1 and GR activity...

Ngày tải lên: 16/03/2014, 18:20

... were fixed and double-stained with polyclonal antip35 (C-19) and anti-AT8 Ig (a, c, and e) and with polyclonal antip35 (N-20) and anti-AT8 Ig (b, d, and f) a and b expressed transfected p25 and p35, ... combination of N-terminal and C-terminal truncations of p35 produces a nonactivating fragment (154–279, CIP) that inhibits Cdk5 activity and binds with high affinity (C) Inhibition of Cdk5 activity ... repositioning of its T-loop by interactions between the T-loop (particularly Ile153 of Cdk5) and a hydrophobic ridge and pocket formed by the C-terminal portions of the a3 and a6 helices of p25 [21],...

Ngày tải lên: 23/03/2014, 21:21

... component of the physical activity and QOL relationship Physical Activity, Quality of Life, and Demographics As noted earlier, relationships among physical activity and quality of life have been examined ... 0.9% of disability limitations data (n = 3), 0.3% of self-efficacy data (n = 1), 1.9% of GLTEQ physical activity data (n = 6), 1.9% of Page of (page number not for citation purposes) Health and ... contributions to conception and design, acquisition of data, analysis and interpretation of data, have been involved in drafting and revising the manuscripts, and given final approval of the version to...

Ngày tải lên: 18/06/2014, 19:20

... vitro antioxidant activity of rutin-loaded nanostructures and ethanolic solution after 30 of UV irradiation 123 120 100 Active release (%) The antioxidant activity (radical scavenging activity) of ... evaluate the influence of the association of rutin to different nanostructures (nanocapsules and nanoemulsions) on its in vitro antioxidant activity and UV photostability Nanocapsules and nanoemulsions ... 1604 of free radicals and chelation of transition metals, acting both in the initiation step and in the propagation of oxidation [6] The intermediate species formed by the action of antioxidant...

Ngày tải lên: 21/06/2014, 17:20

... depletion and were reversible within months of drug withdrawal The pharmacokinetic profile of one or two doses of belimumab was dose proportional and consistent with a fully human mAb A half-life of ... initiation of phase II studies investigating the safety and clinical activity of belimumab in patients with SLE and rheumatoid arthritis Competing interests JZ, WC, and WF were all employees of Human ... placebo and 55 (97%) patients treated with belimumab (Table 3) The majority of AEs were mild to moderate in severity, and the incidence of AEs was similar in the placebo and belimumab (single and...

Ngày tải lên: 09/08/2014, 13:21

... absorbance in the presence of the sample and H2 O2 , A0 is the absorbance of the control in the absence of the sample and H2 O2 , and A is the absorbance only in the presence of the H2 O2 2.6 Nuclear ... elucidation of TMP-A The intensity of bands around 3408.22 cm−1 in the IR spectrum (Fig 1) was due to the hydroxyl stretching vibration of the polysaccharide and as expected they were broad The bands ... important for the protection of living systems Fig shows the percentage hydroxyl radical scavenging effects of TMP-A at the dose of 0.5, 1, 2, 3, 4, and 10 mg/mL and IC50 value of TMP-A was about 7.1...

Ngày tải lên: 28/06/2014, 11:20

... percentage inhibition of LDL oxidation for the standards like BHA and AA was also determined and compared with those of the synthesised compounds (Fig 3) The antioxidant activity of BHA and AA was still ... SD (n = 3) Evaluation of In Vitro Antioxidant Activity 90 antioxidant activity of 5H-dibenz[b,f]azepine and its derivatives on human LDL oxidation and lipid peroxidation activity in the liposome ... mass spectroscopy and elemental analysis The IR spectra of compounds (e) and (f) showed the absent of the N–H absorption band at 3400 cm–1 and the presence of the C=O stretching band at 1600 cm–1,...

Ngày tải lên: 07/08/2014, 14:20

... doses of 40 and 80 µmol/kg reduces the activity by 39 and 82% respectively (Number of experimental animals: 3–6) ii In apomorphine pretreated rats, TPBIA (80 µmol/kg) reverses the hyperactivity and ... effects of TPBIA with specific focus on neuroleptic effects, 3) to test its antioxidant activity Figure The Ugo-Basile activity cage (type 7401) The Ugo-Basile activity cage (type 7401) Materials and ... (Number of experimental animals: 4) b) modifies the motor behavior of the experimental animals, c) shows antioxidant activity a) The Polar Surface Area (PSA) of a molecule is defined as the area of...

Ngày tải lên: 08/08/2014, 20:23

... hepatic (A) CAT activity; (B) SOD activity; (C) GPx activity; (D) protein expression of CAT, SOD and GPx; (E) representative Western Blot images of the protein expressions of CAT, SOD and GPx in STZ-diabetic ... Effects of metformin, baicalin and metformin + baicalin on the hepatic (A) SOD activity; (B) CAT activity; (C) GPx activity; (D) quantifications of the protein expressions of CAT, SOD and GPx ... (GPx) and catalase (CAT) are other antioxidant enzymes that catalyze the conversion of hydrogen peroxide to water Decreases in expression, and in some instances the inactivity of each of these antioxidant...

Ngày tải lên: 12/09/2015, 10:58

... = a.V 100% m100  w) V: volume of extracted liquid, mL m: mass of sample, g w: moisture 2.5 Verification of antioxidant activity and IC50 index The Antioxidant activity was calculated by: AA% ... temperature and distilled water ‒ The IC50 index of Basella rubra L was 1.8 µg/mL, profusely lower than garlic acid and ascorbic acid The lower of IC50 the more effective of antioxidant activity, ... Souza, R N Almeida and T S Assis 2012 Total Phenolic Content and Antioxidant Activity of Some Malvaceae Family Species Antioxidants, 1:33-43 Pawlowicz, P 2000 Administration of natural anthocyanins...

Ngày tải lên: 06/10/2015, 15:58

... Institute of Technology I want to particularly mention Åke Uhlin and Anders Nilsson for encouragement and support in an early stage of my doctoral studies, and Berthel Sutter for reading and commenting ... content, and methods in three integrated groups are compared and analyzed The analysis is based on staff and parent understanding and evaluation of methods and content The data consists of interviews, ... goal of integration were taken in 1996 and 1998: the authority of the child care institutions was transferred from the National Swedish Board of Health and Welfare to the Ministry of Education and...

Ngày tải lên: 16/01/2014, 16:33

... proportional random selection of primary and secondary sampling units (towns and sections, respectively), with the final units (individuals) being selected by means of random routes and sex- and age-based ... contributions DPC conceived of the study, and participated in its design and coordination and draft the manuscript CAB carried out the acquisition of the data, analysis and interpretation of data She has ... help to prevent physical inactivity and improve the health status of older people in Spain List of abbreviations PA: Physical activity; LTPA: Leisure time physical activity; SNHS: The Spanish...

Ngày tải lên: 14/02/2014, 06:20

... of genetic engineering of the secondary binding site of the B subtilis and A niger xylanases on the screening ratio The screening ratio is defined as the ratio of activity on Xylazyme AX and activity ... NMR-monitored titrations of Bacillus circulans xylanase [13] and X-ray analysis of crystals of catalytically incompetent mutants of the xylanases of Bacillus subtilis (XBS) and Aspergillus niger ... reflected by the activity measurements Unexpectedly, Kd values of D32A are higher than those of the wild-type, whereas the activity of the mutant was unaffected Comparison of XBS and XAN In general,...

Ngày tải lên: 14/02/2014, 19:20

... between EGF-induced reduction in Bid and tBid levels and the ubiquitin ligase activity of Itch In this study, we first examined the ability of Itch to interact with Bid and tBid We found that Itch specifically ... inset) n = conditions [29] Treatment of HEK-293T cells with TRAIL is known to induce caspase activity and cleavage of Bid in tBid [30] In our hands, treatment of HEK-293T cells with 200 ngÆmL)1 ... ± 0,02% of control; P = 0.001 for cell survival and 0.94 ± 0.03 of control for caspase activity) , and treatment with EGF slightly increased this effect on cell survival (88.4 ± 2.0% of control;...

Ngày tải lên: 16/02/2014, 09:20

... pH 7.0 and °C for h, followed by SDS ⁄ PAGE The rate of ligation was estimated from percentage intensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and 19-kDa ... at pH 7.0 and °C for indicated periods of time Lane c, the nicked precursor was incubated at pH 7.0 and °C for 24 h and then dialyzed overnight at °C and pH 4.0 (B) Effects of addition of different ... different amounts of the 38-kDa mature form (s) and the wild-type enzyme (d) on the rate of ligation Catalytic and substoichiometric amounts (molar ratio of 0.01 and 0.1, respectively) of these proteins...

Ngày tải lên: 19/02/2014, 07:20

... behaviour and a low unstable inhibitory activity, and for I137A a biphasic loss of activity (Fig and Table 3) The phenotype of these substitutions is therefore different from that of N139A, T146A and ... underlined) allows radioactive labelling of the molecule, a feature not used in the present study The reactivity of His- and HMK-tagged PAI-1 in terms of uPA inhibition and vitronectin binding was not ... addition of 25 lL 0.3 mgÆmL)1 S-2444 (Chromogenix, Sweden) and further incubation for 40 The specific inhibitory activity of PAI-1 at the various timepoints, i.e the fraction of the total amount of...

Ngày tải lên: 20/02/2014, 11:20

... a ratio of : : 0.01 (v/v/v) and sprayed at a flow rate of lLÆmin)1 MALDI-TOF MS of LPS and free lipid A were performed on a Reflex III (Bruker-Daltonik, Bremen, Germany) in the linear TOF configuration ... from C trachomatis serotype E and L2 were of similar activity and both less active than smooth LPS by a factor of 100 whereas, LPS from Chl psittaci was of lower activity than the two other chlamydial ... complex pattern of several groups of ion peaks representing molecular ions (centered around m/z 2510, 2300 and 2005) and of laser-induced fragment ions [18] representing the lipid A moiety of LPS (centered...

Ngày tải lên: 20/02/2014, 23:20

... form of b strand [18] The positive bands at » 1675 cm)1 in the amide I region of the ROA spectra of b- and j-casein, which originate mainly in the peptide C ˆ O stretch, are characteristic of disordered ... types of loops and turns A negative ROA band in the region » 1238±1253 cm)1 appears to be a reliable signature of b strand, individually or within b sheet, so the well-de®ned negative band at ... the theme of PPII structure and rheomorphism is explored by a comparative ROA study, supplemented with DSC, of caseins, synucleins and tau, together with several mutants of a-synuclein and tau...

Ngày tải lên: 21/02/2014, 15:20