anticancer activity and therapeutic applications of chitosan nanoparticles

... aim of the present study was to determine the effect of different cryoprotectants and their concentration on the physicochemical characteristics of chitosan nanoparticles (CS-NPs) The effect of ... polydispersity and size distributions and zeta potentials All the measurements were done in triplicate and the results were expressed in terms of ratio of Z-average before and after the process of lyophilization ... of aqueous phase into CS-NPs by the mechanism of imbibition and osmosis due to the presence of hydrophilic CS and TPP as excipients in the CS-NPs 3.2 Effect of cryoprotectant on CS-NPs, HA- and...

Ngày tải lên: 24/11/2022, 17:56

... safety and efficacy of cell therapy pro- ducts. The greater the understanding of the biology of stem cell self-renewal and differentiation, the more pre- cise the evaluation and prediction of potential ... ableinaverywiderangeofdevelopmentofnewdrugs and new procedures for the treatment of a great number of human diseases. At least 5-10 years will elapse until these products, not therapeutic yet and under ... processing; iv) specific and detailed determination of the type of cells forming the product and what are their exact purity and potency; v) in vivo safety and efficacy of the product. There is...

Ngày tải lên: 18/06/2014, 16:20

... fluorescence imaging, modeling and simulation to examine pharmacokinetics and therapeutic regimen of a novel anticancer prodrug Jing-Hung Wang1, Aaron N Endsley2, Carol E Green3 and A C Matin1* Abstract ... (GDEPT) It holds the promise of largely avoiding offtarget toxicity if the delivery of the gene and activation of the prodrug are confined specifically to the tumor, and considerable effort has ... Sciences of the National Institutes of Health under Award Number UH2TR000902 (to ACM) The content is solely the responsibility of the authors and does not necessarily represent the official views of...

Ngày tải lên: 20/09/2020, 14:10

... cDNA, and lanes 1, 3, and show the same using genomic DNA of C roseus Primer pairs were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and #GSP-4 (lanes and 4); #GSP-2 and #PFLR-1 (lanes and 6); and ... #PFLF-1 and #PFLR-1 (lanes and 8) (B) Schematic organization of the CrPrx gene The asterisk indicates the position of the codon encoding the first amino acid of the mature protein, and the regions of ... membranes of the central vacuole, and the wall thickening of xylem cells (fi) common with other plant peroxidases [18,21,22] The inclusion of Ser96 and Asp99 in a salt bridge motif at the beginning of...

Ngày tải lên: 23/03/2014, 09:21

... to the understanding of the underlying mechanism of calcium metabolism in the process of shell and pearl formation, but also offer the opportunity to promote the yield and quality of pearl In this ... second and third EF-hand domains in oyster CaM and CaLP, while it reflected Ca2+-binding to the first and second EF-hand domains in the case of VanScyoc et al CaLP and CaM chromatography of extracts ... major component of oyster shell as well as a key intracellular second messenger The shells of oyster consist of 90% CaCO3, products of calcium metabolism, and a few percent of matrix of biological...

Ngày tải lên: 16/03/2014, 23:20

... life, and for being my unfailing source of strength and help and hope throughout these years and this thesis i Table of Contents TABLE OF CONTENTS Acknowledgements i Table of Contents ii List of ... µl of 70% ethanol and air-dried The DNA pellet was dissolved in µl of loading dye (50 ml contains ml of 25 mM EDTA, pH8.0; 10 ml of deionised formamide; 50 mg Dextran blue and 39 ml of H2O) and ... for minutes and cooled in ice immediately µl each of dGTP, dTTP and dCTP with 7.5 µl of Random Primers Buffer Mixture and 2.5 µl of 32 P-dATP was added to make up to a total volume of 25 µl after...

Ngày tải lên: 03/10/2015, 20:57

... studies, high affinities and activities of CA had been found in the plume and in the trophosome CA from the branchial plume tissue had an affinity of 13.9 mmolÆL)1 and an activity of 253.7 lmol CO2Æmin)1Æg)1 ... branchial plume cDNA with an open reading frame of 726 nucleotides and 5¢- and 3¢ UTR sequences of 171 and 442 nucleotides, respectively Positions of the primers on the complete cDNA are given ... previously sequenced RpCAtr (BPNJ ¼ 100 and BPMP ¼ 99) Fungia scutaria (FCA-a and FCA-b) and Caenorhabditis elegans (CA1 and CA2) sequences fall outside of clade I and are more closely related to each...

Ngày tải lên: 18/02/2014, 16:20

... analysis of metabolites Reactions were initiated by the addition of 100 ll of a solution of enzyme (200 lgÆmL)1) to 0.9 mL of a solution of 50 lg of substrate in 10% (v/v) dimethyl sulfoxide and incubated ... measurement of activity is described above To examine the enzymatic incorporation of 18O2 and 18O-labeled water into GG, reactions were initiated by the addition of 20 lL of a Enzymic reaction and analysis ... 0.1 mL of EC or CY to 1.0 mL of VM containing 10 lg of GOU For HP and M, reactions were initiated by addition of 0.1 g of HP or M to 1.0 mL of VM containing 10 lg of GOU After incubation for 12...

Ngày tải lên: 17/03/2014, 03:20

... ACF7, neural isoform 2, is a member of the dystonin subfamily and the beta-spectrin superfamily Fig Preparation of an anti-ISP36 serum (A) Expression of human His6-ISP36 in E coli and its purification ... NaCl Animals Animal care, housing and killing were in accordance with the guidelines of the Ministry of Education, Science, Sports and Culture of Japan for the use of laboratory animals Cell fractionation ... Tween-20 for each, and detected using H2O2 and 3,3¢-diaminobenzidine This work was supported by a grant-in-aid from the Ministry of Education, Science, Sports and Culture of Japan and grants from...

Ngày tải lên: 30/03/2014, 20:20

... signals were band-pass filtered with a 5th order Butterworth filter with a cut-off frequency range of 5-100 Hz The low cut-off attenuates the effects of movement [30], while the high cut-off attenuates ... Alves and Chau Journal of NeuroEngineering and Rehabilitation 2010, 7:22 http://www.jneuroengrehab.com/content/7/1/22 Page of 10 Figure Signal denoising The microphone signal and RMS values of the ... initial burst of activity facilitates the detection of contraction onset, the eventual decay in activity encumbers activity- detection during sustained contractions This limits the potential of a secondary...

Ngày tải lên: 19/06/2014, 08:20

... basis of our data this might be a consequence of the rapid conversion of CGA to DCQs as by means of an unknown downstream enzymatic step Indeed the involvement of the HQT gene in the profile of ... distances on LG5 and LG9 have been improved with the integration of the HQT and HCT genes The former increased the number of bridge markers on LG5, and reduced some large gaps (of 10 cM and cM) affecting ... Figure A simplified diagram of enzymes and major products in the synthesis of chlorogenic acid in plants A simplified diagram of enzymes and major products in the synthesis of chlorogenic acid in...

Ngày tải lên: 12/08/2014, 03:20

... and treat infections Traditionally, the generation of new drugs involves screening hundreds of thousands of components against desired targets via in vitro screening and appropriate in vivo activity ... virus efficiently and the higher the concentration of the recombinant phages, the higher the signal irrespective of the concentration of the virus Antiviral activity of peptides and fusion phages ... eluted and titered [Percentage of phage binding = (number of phage bound in the presence of peptide competitor/number of phage bound in the absence of peptide competitor) × 100] In vivo study of...

Ngày tải lên: 12/08/2014, 04:21

... Figure contribution of unequal crossover and retroposition Relative Relative contribution of unequal crossover and retroposition 'TAG/Retro' is the ratio of the cumulative number of tandemly arrayed ... results of this study will further our understanding of the mechanisms of gene duplication in mammals Pan and Zhang R158.3 comment of the human genome [32], and usually not contain genes [33] Third, ... linear functions of which are Hn and Mn (red) The FAM2 gene set was plotted in bold lines, the linear functions of which are Hp1 and Mp1 (red) for the part with Ks ≤ 0.25, and Hp2 and Mp2 (green)...

Ngày tải lên: 14/08/2014, 08:20

... activity of the mammalian target of rapamycin (mTOR) is a major inhibitor of the progression of apoptosis, and stability of mitochondrial function may result from PINK-1-induced upregulation of Akt activity ... thorough overview of pathology and cell- and signal-specific mechanisms of survival and repair in a clinically relevant rodent SCI model Xiao-Ming Xu, Ph.D., Chair v TABLE OF CONTENTS List of Tables ... of inflammatory and foreign molecules and cells into the typically secluded parenchyma of the CNS (Tator and Fehlings, 1991, Casella et al., 2006) Currently, the involvement and interaction of...

Ngày tải lên: 24/08/2014, 09:14

... suspension with particle size of 100 and 150 nm and induction of apoptosis Ye HQ et al., College of Life Science and Technology, Huazhong University of Science and Technology, PRC Myeloid leukemia ... JZ and Ho PC, 2006] Inhibition of growth and promotion of apoptosis Observations of the clinical utility of realgar in the treatment of APL have triggered investigations into the mechanisms of ... outcomes of ATO and realgar in the treatment of leukemia and low toxicity of orpiment compared to ATO, and turned to orpiment, exploring its potential therapeutic effects for the treatment of cancer...

Ngày tải lên: 11/09/2015, 16:03

... Chromatograms of Arginine (a), hydrolysis product of soluble GRGDS peptide (b) and soluble galactose ligand (c) as standards, and hydrolysis product of the PET-Hybrid conjugated with GRGDS peptide and ... metabolic profiles and toxicity of hundreds of thousands of drug-like chemical compounds in order to screen out the promising candidates which can then enter the following animal testing and pre-clinical ... (I) therapeutic applications which involve the generation of functional tissue grafts in vitro and implantation in vivo to replace the functions of corresponding tissues and (II) diagnostic applications, ...

Ngày tải lên: 12/09/2015, 08:19

... in Iceland and Japan and highest in USA and France The overall prevalence was the lowest in Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique The burden of SLE ... humor, kindness, and help Thanks to all staff of Deparment of Rheumatology and Immunology, Singapore General Hospital for their help and friendship ii Thanks to all staff of Department of Clinical ... THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2007 ACKNOWLEDGEMENT First of all, I would like to express...

Ngày tải lên: 14/09/2015, 12:42