antibody gpcr ligands and tyrosine sulfation on hiv 1 infection

... synthesis and binding to HIV- 1 gp120 Bioorg Med Chem 2006, 14 :11 82 -11 88 Sattentau QJ, Dalgleish AG, Weiss RA, Beverley PC: Epitopes of the CD4 antigen and HIV infection Science 19 86, 234 :11 20 -11 23 ... 77 :10 119 -10 124 Freed EO: HIV- 1 and the host cell: an intimate association Trends Microbiol 2004, 12 :17 0 -17 7 Gomez C, Hope TJ: The ins and outs of HIV replication Cell Microbiol 2005, 7:6 21- 626 ... peripheral blood mononuclear cells (PBMCs) in vitro (Fig 1A) After incubation with HIV- 1 (IIIb or BaL) for h, TE cells from some donors (nos 14 4, 19 5, 19 6, and 11 01) appeared to capture and transfer...

Ngày tải lên: 13/08/2014, 05:21

... HIV- 1 + 9G8 0 ,10 0,05 1: 0.5 1: 1 1: 3 1: 0.5 1: 1 1: 3 +9G8 1: 3 +SC35 1: 1 +ASF/SF2 B HIV- 1 1:0.5 Pr55Gag p48 p 41 αCAp24 CAp24/p25 p66 p 51 gp160 αRT αVpr αTMgp 41 TMgp 41 Figure Expression of viral proteins ... suboptimal 3' splice site and a 10 nucleotide exon Page 12 of 13 (page number not for citation purposes) Retrovirology 2005, 2:33 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 splicing silencer ... of 13 (page number not for citation purposes) Retrovirology 2005, 2:33 http://www.retrovirology.com/content/2 /1/ 33 A VIRION CAp24 (ng/µl) 0,20 HIV- 1 HIV- 1 + ASF/SF2 HIV- 1 + SC35 0 ,15 HIV- 1 +...

Ngày tải lên: 13/08/2014, 09:21

... without AZT (A) (B) 10 6 0 .14 10 5 1. 2x105 1x105 3.6x104 10 4 8.7x103 10 3 5x102 10 10 pNL ssDNA U3 FL FSpl intravirion (DNA/RNA) x100 intravirion DNA copy numbers 0 .1 0 .12 0 .10 0.08 0.06 0.06 0.04 ... recombination between FL and spliced forms and thus the infectious HIV recovery http://www.retrovirology.com/content/4 /1/ 30 10 11 Abbreviations HIV- 1, human immunodeficiency virus type 1, FL, fulllength, ... preintegrated HIV DNA Science 20 01, 293 :15 03 -15 06 Wu Y: HIV- 1 gene expression: lessons from provirus and nonintegrated DNA Retrovirology 2004, 1: 13 Saurya S, Lichtenstein Z, Karpas A: Defective rev response...

Ngày tải lên: 13/08/2014, 09:21

... containing C95A HIV- 1 protease Structural features of C95A mutation in HIV- 1 protease Eight structures deposited in the PDB (files 1DAZ, 1A94, 1HWR, 1HVR, 1QBS, 1DMP, 1QBR and 1QBT) are of complexes ... 270) 12 35 Table Rmsd values for pair-wise superposition of unliganded C95M/C1095A structure with each of the six cyclic urea-complexed structures shown in Fig 2B 1LV1 1LV1 1HWR 1DMP 1QBS 1HVR 1QBR ... atoms in the S1 pocket on Fig Superposition of peptide inhibitor-bound C95A HIV- 1 protease in PDB file 1DAZ (shown by green carbons) on unliganded HIV- 1 protease structure in PDB file 1LV1 (shown by...

Ngày tải lên: 31/03/2014, 07:20

... anticoagulant tubes, and plasma aliquots were stored frozen at -80°C HIV contained in plasma and vaginal secretion supernatant samples was collected by centrifugation and virion-associated HIV- 1 RNA copies ... Genotypes (n = 14 ) p-value* 62 .1% 14 .3% 0. 01 20.7% 71. 4% 0.004 48.3% 44.4% 1. 00 41. 4% 42.9% 0. 81 38.0% 34.6% 85.7% 44% 0. 01 0.44# *Chi Square (Χ2) analyses #Fisher Exact Test; data from 81% of the ... phenotype of HIV- 1 variants in saliva and blood during primary infection Aids 2003, 17 (14 ):2025-2033 Freel SA, Williams JM, Nelson JA, Patton LL, Fiscus SA, Swanstrom R, Shugars DC: Characterization of...

Ngày tải lên: 20/06/2014, 01:20

... RT and protease sequences from persons infected with nonsubtype B HIV- 1 Program and abstracts of the 10 th Conference on Retroviruses and Opportunistic Infections; February 10 14, 2003; Boston, ... Page of (page number not for citation purposes) Journal of the International AIDS Society 2005, 7: 71 10 11 12 13 14 15 http://www.jiasociety.org/content/7 /1/ 71 Kantor R, Katzenstein D, Camacho ... patterns and clinical response among non-B HIV- 1 For new drugs and certain combinations, there are insufficient data, even in subtype B HIV- 1 Worldwide collaboration, using common data collection instruments...

Ngày tải lên: 20/06/2014, 08:20

... Research and Therapy 2004, 1: 2 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Anderson J, Banerjea A, Akkina R: Bispecific short hairpin siRNA constructs targeted to CD4, CXCR4, and CCR5 confer HIV1 ... inhibitor of HIV- 1 replication Proc Natl Acad Sci USA 2002, 99 :14 047 -14 052 Rossi J: The application of ribozyme to HIV- 1 infection Curr Opin Mol Ther 19 99, 1: 316 -322 Coburn GA, Cullen BR: Potent and specific ... the HIV- 1 central flap sequence and the wood- Page of 11 (page number not for citation purposes) AIDS Research and Therapy 2004, 1: 2 http://www.aidsrestherapy.com/content /1/ 1/2 Figure HIV- 1 challenge...

Ngày tải lên: 10/08/2014, 05:20

... Pendleton Charitable Trust and the American Lebanese Syrian associated Charities (ALSAC) References 10 11 12 13 14 15 16 17 18 Perrin L, Kaiser L, Yerly S: Travel and the spread of HIV- 1 genetic ... Syndr 19 94, 7: 211 - 219 Richman DD, Wrin T, Little SJ, Petropoulos CJ: Rapid evolution of the neutralizing antibody response to HIV type infection Proc Natl Acad Sci U S A 2003, 10 0(7): 414 4- 414 9 ... Dawson D, Lockey TD, Hurwitz JL: Does the key to a successful HIV vaccine lie among the envelope sequences of infected individuals? Aids Res Hum Retroviruses 19 95, 11 :11 31- 113 3 Seward JF, Watson...

Ngày tải lên: 10/08/2014, 05:20

... T cells* C14 >10 >10 >10 HIV- 1Ba-L 1. 3 ± 01 HIV- 1NDK 0.02 ± 0.0 HIV- 1Ba-L 1. 3 ± 0 .1 HIV- 1NDK 1. 8 ± 0 .1 HIV- 1Ba-L 0.8 ± 0.0 HIV- 1NDK 0.7 ± 0 .1 Enfuviritid (T20) >10 >10 >10 0.08 ± 0 .1 ± 0.5 0.3 ... http://www.aidsrestherapy.com/content/5 /1/ 10 10 11 Effects of low and mucosa in the New Zealand20 μM) of C14 Figure model on cervicovaginal high concentrations (1 and White rabbit Effects of low and high concentrations (1 ... control wells containing triton X100-treated HIV- 1 and activated PBL (negative control) Figure into primary C14 Evaluation of cells inhibitory activity on HIV- 1 DNA content Evaluation of C14 inhibitory...

Ngày tải lên: 10/08/2014, 05:21

... Research and Therapy 2009, 6:20 10 11 12 13 14 15 16 17 18 19 20 21 Brenchley JM, Schacker TW, Ruff LE, Price DA, Taylor JH, Beilman GJ, et al.: CD4+ T cell depletion during all stages of HIV disease ... type infection and substantial delay in restoration following highly active antiretroviral therapy J Virol 2003, 77 :11 708 -11 717 Pope M, Haase AT: Transmission, acute HIV- 1 infection and the quest ... N/A 15 20 XX186 9/24/2008 N/A 839 XX148 9 /17 /2008 N/A 724 XX0 21 8 /19 /2008 N/A 845 XX280 8/26/2008 10 03 923 XX495 8 /16 /2008 354 71 238 XX326 8/23/2008 2 014 9 392 XX286 9 /17 /2008 2974 320 XX 119 8/26/2008...

Ngày tải lên: 10/08/2014, 05:21

... attention as an alternative strategy for HIV prevention [11 ,12 ] Infection prevention agents, such as vaginal microbicides, must be controlled by women [13 ] and provide a defense against HIV infection ... participated in the conception and experimental design of the in vitro HIV- 1 manipulation and infection assays, in the analysis and interpretation of the data, and in the writing and revision of this report ... 60 :18 1 -19 2 76 Borkow G, Lara HH, Lapidot A: Mutations in gp 41 and gp120 of HIV- 1 isolates resistant to hexa-arginine neomycin B conjugate Biochem Biophys Res Commun 2003, 312 :10 47 -10 52 77 Montefiori...

Ngày tải lên: 11/08/2014, 00:22

... Charo IF, Herndier BG, Speck RF, Gold- Page 11 of 12 (page number not for citation purposes) Retrovirology 2009, 6:43 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 smith MA: Progress toward ... molecular clones of HIV- 1LAI, HIV- 1MAL, and HIV1 ELI Virology 19 91, 18 5:6 61- 672 Collman R, Balliet JW, Gregory SA, Friedman H, Kolson DL, Nathanson N, Srinivasan A: An infectious molecular clone of ... http://www.retrovirology.com/content/6 /1/ 43 the HIV- 1 LTR, to examine the effect of hCycT1 and hCRM1 on Tat-directed gene expression Expression of hCycT1, but not hCRM1, enhanced LTR-derived expression several fold, consistent...

Ngày tải lên: 12/08/2014, 23:20

... of Possible Initial Prop Visits 1, 5 0.80 44 12 49 50 54 1* 1* 58* env env env pol pol env env* env* env* 1 1 1 1 1, 6 1, 4 ,11 1, 3,5 1, 5 1, 2,8 1, 1, 10 1, 10 1, 3 1 1 1 1 3 2 2 0.80 0.20 0.20 0.40 0.40 ... 0.60 0.30 0.57 0.50 0.22 39 pol 1, 7 1 1.00 0 43 45 pol env 10 2,3 1, 5,6 ,10 1 1 1. 00 1. 00 15 pol 1, 4 1 0.20 1 20 13 17 55 pol pol env pol 1, 4,9 1, 1, 6 1, 2 1 1 1 1 0.20 0.50 0.80 0.20 Average: Superinfected ... 0.20 0.20 58* env* 1, 3 1, 3 2 0.20 19 env 1, 2 ,10 1, 2 ,10 3 0.60 23 env 3,4,6 0.90 38 10 * 10 * 10 * 14 pol env* env* pol env pol 5,8 3 3,5,8,9 1, 3,5,7 1, 3,5,7 1, 3 1, 9 ,11 1, 3,6 1 1 3 2 0.20 0.60 0.30...

Ngày tải lên: 12/08/2014, 23:21

... (1. 9) 17 8 (7.9) 25 (2.2) 69 (6 .1) 19 (1. 7) 10 9 (9.6) (1. 7) 42 (8.8) 11 (1. 7) 67 (10 .2) HHD 370 (16 .3 19 0 (16 .8) 18 0 (15 .9) 75 (15 .6) 10 5 (16 .1) HHE 310 (13 .7) 13 8 (12 .2) 17 2 (15 .2) 61 (12 .7) 11 1 ... 203 1. 1 0.85 -1. 46 0. 417 10 4 1. 1 0.77 -1. 56 0. 61 99 1. 2 0.75 -1. 75 0.5 31 HHD 16 7 1. 0 0.74 -1. 32 0.983 94 0.9 0.59 -1. 23 0.385 73 1. 2 0.75 -1. 92 0.442 HHE HHD/HHE 15 8 31 1 .1 1.9 0.82 -1. 51 1 .14 -3 .16 0.495 ... 3.6 2.65-4.93 < 00 01 162 3.0 2.04-4. 51 < 00 01 137 4.6 2.79-7.65 < 00 01 Donor VL (per 1. 0 log10 unit) 523 1. 6 1. 32 -1. 91 < 00 01 263 1. 3 1. 03 -1. 74 0.028 260 1. 8 1. 35-2.47 < 00 01 *N represents the...

Ngày tải lên: 13/08/2014, 01:20

... Retrovirology 2 011 , 8:42 http://www.retrovirology.com/content/8 /1/ 42 SHORT REPORT Open Access Conformational alterations in the CD4 binding cavity of HIV- 1 gp120 influencing gp120-CD4 interactions and fusogenicity ... read and approved the final version Competing interests The authors declare that they have no competing interests Page of 14 15 16 17 18 19 20 21 Received: 20 April 2 011 Accepted: June 2 011 Published: ... of the HIV gp120 envelope glycoprotein Nature 19 98, 393:705- 711 12 Wyatt R, Sodroski J: The HIV- 1 envelope glycoproteins: fusogens, antigens, and immunogens Science 19 98, 280 :18 84 -18 88 13 Sterjovski...

Ngày tải lên: 13/08/2014, 01:20

... AI0 614 82 to W.L Authors’ contributions AR performed the experiments on HIV infection and HIV attachment by HIV p24 ELISA JD performed the experiments on HIV infection and HIV attachment by FACS and ... 2 011 , 8:45 http://www.retrovirology.com/content/8 /1/ 45 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 alpha defensins and LL-37 with genital infections and increased HIV acquisition ... USA 20 01, 98 (18 ) :10 037 -10 0 41 doi :10 .11 86 /17 42-4690-8-45 Cite this article as: Rapista et al.: Human defensins and enhance HIV- 1 infectivity through promoting HIV attachment Retrovirology 2 011 8:45...

Ngày tải lên: 13/08/2014, 01:20

... deamination mediates Page 11 of 13 (page number not for citation purposes) Retrovirology 2008, 5:72 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 innate immunity to retroviral infection ... Vif(-) HIV- 1NL4-3 P24 antigen was measured post-infected day 3, 5, 7, 10 14 , 21 a results of wild type Vif(+) HIV- 1NL4-3 infection; b results of Vif(-) HIV- 1NL4-3 Δ Vif infection Page of 13 (page ... standard errors of the means a results of wild type Vif(+) HIV- 1NL4-3 infection; b results of Vif(-) HIV- 1NL4-3ΔVif infection * p < 0. 01 C Effect of APOBEC3G variants on spread viral infection...

Ngày tải lên: 13/08/2014, 05:21

... http://www.retrovirology.com/content/5 /1/ 97 a RNPs Absorbance 254 nm 0 .18 Monosomes polysomes 0 .16 0 .14 0 .12 0 .1 pcDNA +EDTA 0.08 0.06 0.04 0.02 0 10 15 Fraction number 20 25 b Sam68∆C Sam68∆C +EDTA 11 13 15 17 19 21 11 13 ... cloned Sam68Δ28ΔC Antibodies were generously provided by N Sonenberg, and H Schaal References 10 11 12 13 14 15 16 Schwartz S, Felber BK, Benko DM, Fenyo E-M, Pavlakis GN: Cloning and functional ... in regulation of HIV- 1 alternative RNA splicing Curr HIV Res 2006, 4 (1) :43-55 Hope TJ: The ins and outs of HIV Rev Arch Biochem Biophys 19 99, 365 :18 6 -19 1 Pollard V, Malim M: The HIV- 1 Rev Protein...

Ngày tải lên: 13/08/2014, 05:21

... features in the HIV- 1 repeat region facilitate strand transfer during reverse transcription RNA 20 01, 7 :10 97 -11 14 Bennasser Y, Le SY, Yeung ML, Jeang KT: HIV- 1 encoded candidate micro-RNAs and their ... promoter proximity and upstream sequences EMBO J 19 92, 11 :15 13 -15 24 Weichs an der Glon C, Monks J, Proudfoot NJ: Occlusion of the HIV poly(A) site Genes Dev 19 91, 5:244-253 McCracken S, Fong N, Yankulov ... Transcription ++ ++ ++ ++ ++ Replication ++ ++ - - ++ A B AB Δ39-48 11 -24 Δ39-48 TARwt HIV- LAI TARm HIV- rtTA 11 -24 Figure The HIV- rtTA genome and mutations in the TAR hairpin The HIV- rtTA genome and...

Ngày tải lên: 13/08/2014, 05:21

... U2OSHIVexo U2OSHIVintro + 10 + 11 WL 12 IP + + + + + + + + + IP + + + WL + + + + 400bp 300bp 200bp β-actin 10 11 12 13 14 15 16 17 18 spliced (A+C, 280bp) unspliced (A+B, 372bp) Figure Detection ... ARP40922_T100, 1: 1000) or a gift from Yosef Shiloh and Maayan Salton (Tel Aviv University, Israel, 1: 10000); p17 (NIH AIDS Reference Reagents Program, 1: 1000); GFP (Roche, 11 814 4600 01, 1: 1000); ... Sci 20 01, 11 4:28 91- 2893 16 Cook PR: The organization of replication and transcription Science 19 99, 284 :17 90 -17 95 17 Chakalova L, Debrand E, Mitchell JA, Osborne CS, Fraser P: Replication and transcription:...

Ngày tải lên: 13/08/2014, 01:21