anti nerve growth factor immunosympathectomy

... Cytokine Growth Factor Rev 11, 97–102 Kim, S.J., Park, K., Rudkin, B.B., Dey, B.R., Sporn, M.B & Roberts, A.B (1994) Nerve growth factor induces transcription of transforming growth factor- b1 ... of Smad1 (anti- PS1) and Smad2 (anti- PS2) were published previously [31,32] The P-Smad1 antibody shows crossreactivity to phosphorylated Smad3 and can thus be used as an anti- P-Smad3Õ (anti- PS3) ... methods Antibodies and reagents The monoclonal antibody against TGF-b1, -b2 and -b3 (clone #1D11) was purchased from R&D Systems Details of polyclonal antisera against Smad2 (anti- S2), Smad3 (anti- S3)...

Ngày tải lên: 16/03/2014, 16:20

... survival on target derived neurotrophic factors such as nerve growth factor (NGF), brainderived neurotrophic factor (BDNF) or glia-derived neurotrophic factor (GDNF) The same neurodegenerative ... KE, Taglialatela G: Tumour necrosis factor- alpha- vs growth factor deprivation-promoted cell death: different receptor requirements for mediating nerve growth factor- promoted rescue Aging Cell ... nitric oxide synthase; MAPK, mitogen activated protein kinase; NF-κB, nuclear factor kappa B; NGF, nerve growth factor; NO, nitric oxide; nNOS, neuronal nitric oxide synthase; NTR, neurotrophin...

Ngày tải lên: 19/06/2014, 22:20

... of interpretation Angiogenic factors of basic fibroblast growth factor (bFGF) [5,8,9,20], vessel endothelial growth factor (VEGF) [9,10,16,24] and epidermal growth factor (EGF) [24] have been ... Saito, H and Nishiyama, N Effects of pretreatment with basic fibroblast growth factor, epidermal growth factor and nerve growth factor on neuron survival and neovascularization of superior cervical ... Evaluation of periphral nerve regeneration by nerve growth factor locally administered with a novel system J Neurosci Methods 1998, 85, 119-127 30 Varon, S and Conner J Nerve growth factor in CNS repair...

Ngày tải lên: 07/08/2014, 15:20

... Richardson P.M., and Ebendal T Nerve growth activities in rat peripheral nerve Brain Res 1982, 19:57-64 Rush R.A Immunohistochemical localization of endogenous nerve growth factor Nature (London) 1984, ... characterization of nerve growth factor receptor in adult rat brain J Comp Neurol 1989, 290:585-598 Yan Q., Eugene M., and Johnson Jr An immunohistochemical study of the nerve growth factor receptor ... Development and survival responsiveness to brain-derived neurotrophic factor, neurotrophin and neurotrophin 4/5, but not to nerve growth factor, in cultured motor neurons from chick embryo spinal cord...

Ngày tải lên: 07/08/2014, 15:20

... of biologically active recombinant human nerve growth factor Biochem Biophys Res Commun 1990, 171, 116-122 Levi-Montalcini R, Angeletti PU Nerve growth factor Physiol Rev 1968, 48, 534-569 Mata ... 191-202 Angeletti RH, Bradshaw RA Nerve growth factor from mouse submaxillary gland: amino acid sequence Proc Natl Acad Sci USA 1971, 68, 2417-2420 Apfel SC Neurotrophic factors and diabetic peripheral ... axons or myelin in peripheral nerves (sciatic nerve) of the negative control group (Fig 5A) Severe vacuolation was seen in the myelin in peripheral nerves (sciatic nerve) of the positive control...

Ngày tải lên: 07/08/2014, 23:22

... release nerve growth factor and express high-affinity nerve growth factor receptors J Allergy Clin Immunol 1997, 100:408-414 Bonini S, Rasi G, Bracci-Laudiero ML, Procoli A, Aloe L: Nerve growth factor: ... the RT-PCR expression levels of mRNA transcripts of four neurotrophins (nerve growth factor (NGF), brain-derived growth factor (BDNF), and neurotrophin (NT)-3, NT-4) and their corresponding receptors ... functions of neurotrophins in development Cytokine Growth Factor Rev 1998, 9:125-137 Aloe L, Tuveri MA, Carcassi U, Levi-Montalcini R: Nerve growth factor in the synovial fluid of patients with chronic...

Ngày tải lên: 09/08/2014, 14:21

... Lindsay RM, Harmar AJ: Nerve growth factor regulates expression of neuropeptide genes in adult sensory neurons Nature 1989, 337:362-364 29 Skoff AM, Adler JE: Nerve growth factor regulates substance ... expression of nerve growth factor by human intervertebral disc cells Spine 2007, 32:635-642 37 Hattori A, Iwasaki S, Murase K, Tsujimoto M, Sato M, Hayashi K, Kohno M: Tumor necrosis factor is markedly ... 25 μm Page of (page number not for citation purposes) Gene expression and correlation of nerve growth factor and substance P in human nucleus pulposus NGF and substance P demonstrated similar...

Ngày tải lên: 09/08/2014, 14:22

... 270:1326-1331 Wert MM, Palfrey HC: Divergence in the anti- apoptotic signalling pathways used by nerve growth factor and basic fibroblast growth factor (bFGF) in PC12 cells: rescue by bFGF involves ... incubated with anti- Flt-1 (sc-316), anti- Flk-1 (sc-504) or anti- Neuropilin (sc-5541) antibodies (2 µg/ml, Santa Cruz Biotechnology) in blocking buffer at 4°C o/n Unbound primary antibodies were ... be required for differentiation of PC12 cells Comparison of the effects of nerve growth factor and epidermal growth factor Biochem J 1992, 288 ( Pt 2):351-355 Publish with Bio Med Central and...

Ngày tải lên: 11/08/2014, 08:20

... ELISA, enzyme-linked immunosorbent assay; FLS, fibroblast-like synoviocyte; NGF, nerve growth factor; NGF-R, nerve growth factor receptor; OA, osteoarthritis; PsA, psoriatic arthritis; RA, rheumatoid ... M: Nerve growth factor and receptor expression in rheumatoid arthritis and spondyloarthritis Arthritis Res Ther 2009, 11:R82 Raychaudhuri SP, Raychaudhuri SK: The regulatory role of nerve growth ... with cytokines tumor necrosis factor- alpha (TNF-α) (1 ng/mL) and interleukin-1-beta (IL-1β) (1 ng/mL) Data are expressed as mean ± standard deviation Nerve growth factor (NGF), TrkA, and p75 data...

Ngày tải lên: 12/08/2014, 14:21

... hippocampal cultures xii Chapter Chapter Introduction Chapter 1.1 Nerve Growth Factor (NGF) Nerve growth factor (NGF) is among the first growth factor to be identified and characterized (Cohen et al., ... well as multiple system failure However, nerve growth factor (NGF) activity was first discovered in snake venom and two sarcoma tissues The nerve growth factor from Naja sputatrix has been purified ... formation in PC12 cells relative to the mouse nerve growth factor Two cDNAs encoding isoforms of NGF have been cloned and an active recombinant nerve growth factor, sputa NGF has been produced in E...

Ngày tải lên: 14/09/2015, 11:25

... Construction of Nerve Growth Factor Loop 4-Containing Polypeptides for Facilitated Gene Transfer to Neurons Jieming Zeng ... brain-derived neurotrophic factor BSA bovine serum albumin CR cysteine-rich cluster DMEM Dulbecco's modified Eagle's medium DRG dorsal root ganglia EGF epidermal growth factor ERK extracellular ... herpes simplex virus Ig immunoglobulin LLR leucine-rich region MND motor neuron disease NGF nerve growth factor NLS nuclear localization sequence NPC nuclear pore complex NT-3 neurotrophin-3 NT-4/5...

Ngày tải lên: 16/09/2015, 17:13

... blocking with BSA The primary antibodies used were: rabbit antimouse GFAP (1: 1000, Sigma), rat anti- mouse Mac-1 (1: 250, Serotec, Oxford, United Kingdom), rabbit anti- http://www.jneuroinflammation.com/content/1/1/11 ... films Three antibodies were used: rat anti- mouse GFAP (1: 5000, clone MAB 2.2B10, provided by Virginia Lee); mouse antiAPP C-terminus (1: 1000, clone C1/6.1, ref 24), and rabbit anti- human actin ... overexpressing transforming growth factor- beta Am J Pathol 1995, 147:53-67 Wyss-Coray T, Lin C, Sanan DA, Mucke L, Masliah E: Chronic overproduction of transforming growth factor- beta1 by astrocytes...

Ngày tải lên: 19/06/2014, 22:20

... as a potent inhibitor of hepatocyte growth factor activator (HGFA), a blood coagulation factor XII-like serine protease that converts pro-hepatocyte growth factor (pro-HGF) to the active form ... Morimoto Y & Kitamura N (1995) Activation of hepatocyte growth factor by two homologous proteases, blood coagulation factor XIIa and hepatocyte growth factor activator Eur J Biochem 229, 257–261 Miyazawa ... human hepatocyte growth factor Biochem Biophys Res Commun 163, 967– 973 Brinkmann V, Foroutan H, Sachs H, Weidner KM & Birchmeier W (1995) Hepatocyte growth factor ⁄ scatter factor induces a...

Ngày tải lên: 15/02/2014, 01:20

... for amphiregulin, EGF and transforming growth factor- a are viable [4] These ligands are synthesized as transmembrane proteins, and soluble ligands (growth factors) are released into the extracellular ... activation of the kinase domain of epidermal growth factor receptor Cell 125, 1137–1149 Voldborg BR, Damstrup L, Spang-Thomsen M & Poulsen HS (1997) Epidermal growth factor receptor (EGFR) and EGFR mutations, ... Mitsudomi T & Yatabe Y (2007) Mutations of the epidermal growth factor receptor gene and related genes as determinants of epidermal growth factor receptor tyrosine kinase inhibitors sensitivity...

Ngày tải lên: 16/02/2014, 09:20

... patients treated with monoclonal antibodies directed against the EGFR Cancer Treat Rev 35, 262–271 Peeters M, Price T & Van Laethem JL (2009) Antiepidermal growth factor receptor monotherapy in ... kinase inhibitors (TKIs) and triggered the development of this class of agents for targeting growth factor receptors frequently expressed in epithelial cancers [9] Two such inhibitors of the tyrosine ... EGFR-TKIs have been shown to inhibit EGFR phosphorylation, as well as the growth of NSCLC cell lines harboring the EGFR-targeted anticancer therapy T790M mutation of EGFR [32,33] Future clinical trials...

Ngày tải lên: 16/02/2014, 09:20

... of epidermal growth factor family in the regulation of postnatal somatic growth Endocr Rev 28, 284–296 Henson ES & Gibson SB (2006) Surviving cell death through epidermal growth factor (EGF) ... immunoprecipitated with an anti- GFP IgG and blotted with a monoclonal anti- Myc IgG to reveal tBid ubiquitylation Cell lysates were further blotted with anti- GFP to assess for tBid–GFP expression, and anti- FLAG ... immunoprecipitated with anti- FLAG and blotted with anti- FLAG and anti- GFP to show total protein coimmunoprecipitation of Itch and tBid (middle panels) The second half was immunoprecipitated with anti- GFP and...

Ngày tải lên: 16/02/2014, 09:20

... with rate of recovery from stroke A variety of angiogenic factors such as VEGF, fibroblast growth factor (FGF) and platelet-derived growth factor are secreted from neuronal cells, astrocytes and ... VEGF–Trap, anti- VEGFR neutralizing IgG and immunotherapy against VEGFRs are undergoing clinical trials Table Activities of vascular endothelial growth factor (VEGF) family members PlGF, placenta growth ... Murohara T (2007) Therapeutic angiogenesis using novel vascular endothelial growth factorE ⁄ human placental growth factor chimera genes Arterioscler Thromb Vasc Biol 27, 99–105 17 Arany Z, Foo...

Ngày tải lên: 18/02/2014, 11:20

... (1993) Role of insulin-like growth factors in embryonic and postnatal growth Cell 75, 73–82 26 Holt RIG (2002) Fetal programming of the growth hormone–insulin-like growth factor axis Trends Endocrinol ... J (1985) Insulin-like growth factors and insuln: comparative aspects Diabetologia 28, 485–493 Clemmons DR (1989) Structural and functional analysis of insulin-like growth factors Br Med Bull 45, ... Metab 12, 48–52 22 Yakar S, Liu J-L & LeRoith D (2000) The growth hormone ⁄ insulin-like growth factor- I system: implications for organ growth and development Pediatr Nephrol 14, 544–549 23 Satake...

Ngày tải lên: 18/02/2014, 13:20

... phospho-specific antibody (clone 36), p38a antibody (clone 27), MKP2 antibody (clone 48) and pan-JNK ⁄ SAPK1 antibody (clone 37), from BD Transduction Laboratories (San Jose, CA, USA); MKP-1 antibody ... Cruz, CA, USA); a-tubulin antibody (clone B-5-1-2) and MAP kinase antibody, from Sigma; phospho-SEK1 ⁄ MKK4 (Ser254 ⁄ Thr261) antibody and phospho-MKK7 (Ser271 ⁄ Thr275) antibody, from Cell Signaling ... (2002) Activation of BAD by therapeutic inhibition of epidermal growth factor receptor and transactivation by insulin-like growth factor receptor J Biol Chem 277, 27643–27650 10 Janmaat ML, Kruyt...

Ngày tải lên: 18/02/2014, 13:20

... studies indicate that growth factors such as nerve growth factor, insulin-like growth factor, fibroblast growth factor, epidermal growth factor (EGF), and hepatocyte growth factor can suppress apoptosis ... preventing it from exerting its proapoptotic effect on mitochondria Nerve growth factor, insulin-like growth factor, and fibroblast growth factor have been reported to transmit survival signals through ... Japan) Antibodies used and their sources were as follows: anti- Bad and anti- Bax, from BD Transduction Laboratories (San Jose, CA); anti( caspase p10) (H-83), anti- (Bcl-XS ⁄ L) (S-18), anti- (Bcl-2)...

Ngày tải lên: 19/02/2014, 06:20