Báo cáo khoa học:The principle of flux minimization and its application to estimate stationary fluxes in metabolic networks docx
... fluxes brought about by thousands of chemical reactions and transport processes. The synthesis of the underlying enzymes and membrane t ransporters causes the cell a certain ÔeffortÕ of energy and external ... anucleated erythrocyte) and the substrates fuelling the reaction are both p resent. On the other hand, despite some s ystematic differences to the r esults of kinetic modelling, the flux-minimization method ... better understand the architecture and regulation of metabolic networks. These approaches have in common that they work without enzyme-kinetic information. Only the s toi- chiometry of the system and, ...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: The Rab5 effector Rabaptin-5 and its isoform Rabaptin-5d differ in their ability to interact with the small GTPase Rab4 doc
... codon and a NotI site. The amplified fragment was sequenced to confirm its authenticity. The amplified frag- ment was used to substitute the 3Â-ends in Rabaptin-5 and Rabaptin-5d cDNAs in the pRn5 and ... between Rab4 and Rabaptin-5d, and this was further supported by results of GST pull-down experiments. Interestingly, both the deletion found in the Rabaptin-5d polypep- tide chain and the Rab4 binding ... Rabaptin-5d, the respective cDNAs were excised from the pRn5 and pRn5d plasmids with XhoI and NotI (the NotI site was blunted after digestion) and cloned between the XhoI and SmaI sites in pEGFP-C2 vector (Clontech....
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Procarboxypeptidase A from the insect pest Helicoverpa armigera and its derived enzyme potx
... released. Interestingly, the sequence at the cleavage point for the release of the octapeptide is also found at the boundary between the activation peptide and the enzyme moieties. The active enzyme ... between the structure of CPAHa in the proenzyme and in the complex which are not reproduced by the model, that the geometry of binding of PCI to CPAHa may differ from that assumed with the model and ... of the activation is also lacking the C-terminal peptide. In Fig. 4B the analysis of the species isolated from the chromatograms in part A of the figure confirms that the protein expressed in the...
Ngày tải lên: 17/03/2014, 03:20
Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf
... will coincide within the limits of experimental accuracy and therefore the heat-capacity change on unfolding will be the same for the wild-type and the mutant protein. The quality of the multiple-curve ... 3.50 and the 4 °CdifferenceinT m between the wild-type and the E34D mutant at both pH 4.00 and 3.75 decreases to only 2 °CatpH3.50.These values, together with the fact that in HUBst, in which the Glu34-Lys13 ... to or lower than the corresponding ones for other nonthermo- philic proteins. Therefore, there seems to be no shift in the stability curve with the hyperthermophilic HUTmar protein. The average specific...
Ngày tải lên: 23/03/2014, 12:20
The French New Wave and the New Hollywood: Le Samourai and its American legacy pptx
... unkempt, and hardly handsome; moreover, as a cop and a surveillance expert respectively, they are ostensibly on the right side of the law, if, in fact, their world is as murky as Julian’s. The French ... films, Thomas Leitch writes: The irreducible complexity of the people pressed into service as suspects and criminals and detectives and the inevitable contamination of the natural resources by cultural ... takes a Tab from the refrigerator and orders a Perrier. They both meticulously construct their identities through costume; Jef’s is the fixed noir uniform of trench coat and trilby, the fetishization...
Ngày tải lên: 23/03/2014, 12:20
acquisition management in the u s air force and its predecessors ppt
Ngày tải lên: 01/04/2014, 18:20
Báo cáo sinh học: " Possible active origin of replication in the double stranded extended form of the left terminus of LuIII and its implication on the replication model of the parvovirus" pptx
... resulting from the transfection of LuIII Lt-Lt/pGlu∆Xba, contrib- uted in the analysis and interpretation of the data, partic- ipated in the idea and design of the models proposed and in the drafting and ... A model for the replication of the (+) and the (-) strand of LuIII is shown. The NS1 nick site and its complementary sequence (*) are indicated. The unpaired sequences present at the left hair- pin ... molecules of the hairpin should be generated for every copy of the stuffer sequence, there- with the intensity of the band corresponding to the hair- pin is expected to be greater than the band corresponding to...
Ngày tải lên: 19/06/2014, 08:20
báo cáo hóa học:" Possible active origin of replication in the double stranded extended form of the left terminus of LuIII and its implication on the replication model of the parvovirus" ppt
... A model for the replication of the (+) and the (-) strand of LuIII is shown. The NS1 nick site and its complementary sequence (*) are indicated. The unpaired sequences present at the left hair- pin ... resulting from the transfection of LuIII Lt-Lt/pGlu∆Xba, contrib- uted in the analysis and interpretation of the data, partic- ipated in the idea and design of the models proposed and in the drafting and ... replication in the double stranded extended form of the left terminus of LuIII and its implication on the replication model of the parvovirus Nanette Diffoot-Carlo*, Lisandra Vélez-Pérez and Idaris...
Ngày tải lên: 20/06/2014, 04:20
báo cáo hóa học:" The impact of iron overload and its treatment on quality of life: results from a literature review" pptx
... contributions JFB and PJ conceived the study and all authors partici- pated in the design of the study. PJ performed the litera- ture review, and LA, DR and JFB drafted and finalized the manuscript. Acknowledgements This ... that the impact of such therapy included pain at the injection site (3/9 [33%]), although one patient reported that the problem was not the pain from the injection site but rather awareness of the ... years old) and continue throughout their life. As a result, the impact on the parent can also be quite high since they have to endure the daily task of inserting a nee- dle into their child and constant...
Ngày tải lên: 20/06/2014, 16:20
The Modulation of Optical Property and its Correlation with Microstructures of ZnO Nanowires ppt
... toward both the understanding and exploitation of its intrinsic properties and the performance in optoelec- tronic applications due to its direct wide band gap of 3.35 eV at 300 K and the high exciton ... is equal to the band gap of ZnO crystal, some electrons in the valence band (VB) can be excited to the conduction band (CB) to form the photo-generated elec- trons in the CB and the same amount ... solution and 60 mg ZnO nanowires. The UV lamp (6 W) was placed in the center of the tube and surrounded by the reactor. Prior to irradiation, the solution was sonicated for 30 min and then stirred...
Ngày tải lên: 22/06/2014, 00:20
Báo cáo hóa học: " The Local Maximum Clustering Method and Its Application in Microarray Gene Expression Data Analysis" docx
... between the results from the four cluster- ing methods and the known clusters of the model data sets. Comparing the matches and agreements between the clustering results and the known clusters of the ... using the K-mean and the self-organized map method, and only compare the agreement between the clustering results with the bottom 6 clusters of the model data sets. Table 2 listed the matches and ... trees, the agreement between the model and re- sult from the hierarchic method is low. The K-mean and the self-organized map methods produce worse matches to the clusters in the models than the...
Ngày tải lên: 23/06/2014, 01:20
Thirst: Everything You Need To Know About Water and Its Importance
Ngày tải lên: 12/07/2014, 20:05
Báo cáo khoa học: "The incidence of recurrent flushing and its effect on branch production in Quercus petraea (Matt) Liebl growing in southern England" ppt
... apart in the Alice Holt Forest, southern England (Harmer, 1991) were felled and returned to the laboratory for further study. The 4 major crown branches and the leading ... shoot were then cut from each tree. The leading shoot was defined as that part of the main stem between the tip and the junction with the main stem of the first large ... growth, further studies should include the timing of reactivation, growth and development of the vascular and root systems and their ability to supply water and nutrients...
Ngày tải lên: 08/08/2014, 23:22
Báo cáo y học: "Dermatology for the practicing allergist: Tinea pedis and its complications" doc
Ngày tải lên: 13/08/2014, 13:22
Báo cáo y học: "The allosteric modulation of lipases and its possible biological relevance" docx
Ngày tải lên: 13/08/2014, 16:21
Báo cáo sinh học: " Identification of gametes and treatment of linear dependencies in the gametic QTL-relationship matrix and its inverse" pptx
Ngày tải lên: 14/08/2014, 13:22