annotation phylogeny genomic distribution and gene expression of poplar tlps
báo cáo khoa học: " The effect of experimental warming on leaf functional traits, leaf structure and leaf biochemistry in Arabidopsis thaliana" pdf
... warming is expected to have profound effects on plant physiology and growth, structure and function of plant populations, species distributions, and probabilities of extinction [6,7] Moreover, ... obtain an Page of 10 integrative understanding of the responses of leaf growth to global warming, we examined the effects of simulated climatic warming on SOD and CAT activities, contents of MDA, proline, ... palisade and spongy tissues) size and number of chloroplasts per cell were determined from 300 cells Chloroplast length and width, area of chloroplast profile, and ratio of total starch grains per chloroplast...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... of GLP-1 (7-37) peptide A sequence of nucleotide including 45 bases was used to connect the 3’ terminus of GLP-1-A gene and 5’ terminus of gAd gene The product of this nucleotide sequence was a ... Doria A Genetic influences of adiponectin on insulin resistance, type diabetes, and cardiovascular disease Diabetes 2007; 5: 1198-209 Hu XB, Zhang YJ, Zhang HT, et al Cloning and expression of adiponectin ... the 5’ terminus of the gene of gAd-GLP-1-A fusion protein, which was used to remove the N-terminus His-tag [9] The gene encoding the gAd-GLP-1-A fusion protein was cloned into the expression vector...
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Tài liệu Báo cáo khoa học: Specific interaction between the classical swine fever virus NS5B protein and the viral genome pdf
... (EMSA) and RdRp assays, and examined the influence of different cis-acting elements on binding of NS5B protein and RNA synthesis in vitro Materials and methods Expression and purification of NS5B ... in 50 lL of reaction mixtures following the standard method: 20 lL of 5· transcription buffer, lL of RNasin (20–40 UÆmL) (Promega), lL of each dNTP (2.5 mM), lg of the template, lL of T7 RNA ... B Fig Expression and RdRp-TNTase assays of the full-length CSFV NS5B protein (A) The NS5B proteins obtained throughout expression and extraction and purification were analyzed on SDS/PAGE and revealed...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... structure (strands B, C and part of strand D), along with part of the distant I strand in the C-terminus region that includes Cys160 and connects to the leftovers of strand D via a disulfide bridge ... of the structural relationship of CP1/CP2 and TP1/TP2 with the remainder of the BLG structure It is evident that all these fragments include a generous portion of the b-barrel structure (strands ... 37 °C and pH 8.1 and the same amounts of enzyme present in the heated mixtures We found 25% and 35% inhibition of trypsin activity on 0.5 mM BAPA when native BLG was added at 0.075 mM and 0.15...
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Báo cáo khóa học: Disruption of the interaction between the Rieske iron–sulfur protein and cytochrome b in the yeast bc1 complex owing to a human disease-associated mutation within cytochrome b potx
... kinetics of reduction of cytochromes b and c1 were measured in the mutant and revertant strains The rates of reduction of cytochromes c + c1, in mutants and revertants, was decreased to 35% of the ... of ISP and the extramembranous cd2 helix of cytochrome b are important for maintaining the structure of the hinge region and, by consequence, the movement of the headgroup and the integrity of ... sequence of the CYTB gene, was constructed by blunt end cloning of a PCR product of CYTB into the pCRscript vector (Stratagene) The mutagenesis was performed using the Quickchange Site-Directed Mutagenesis...
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Báo cáo khoa học: Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi pdf
... Post-transcriptional regulation of gene expression in trypanosomes and leishmanias Mol Biochem Parasitol 156, 93–101 Haile S & Papadopoulou B (2007) Developmental regulation of gene expression in trypanosomatid ... RNA polymerase II is constitutive and genes in the same polycistronic unit display different levels of processed mRNA [4–6] Consequently, the regulation of gene expression in trypanosomatids seems ... important for recruiting the argonaute A No of genes 61 52 31 34 1 1 1 1 1 1 1 1 protein (hAgo2) to SGs and for efficient biogenesis and ⁄ or stability of P-bodies in mammals Thus, it seems probable...
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Cd toxicity effects on growth, mineral and chlorophyll contents and activities of stress related enzymes
... and Khan N S 1983 Influence of lead and cadmium on the growth and nutrient concentration of tomato (Lycopersicon esculentum) and egg-plant (Solanum melongena) Plant Soil 74, 387–394 Khan D H and ... blanks and a reference material (ryegrass BCR 281, Community Bureau of Reference, Commission of the European Communities) treated in the same way Only one determination of the concentration of Cd and ... activation of enzymes already present in plant cells (Van Assche and Clijsters, 1990a) Changes in SOD and POD activities, and in the (iso)SOD and (iso)POD patterns have been reported both in leaves and...
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Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
... monodon As there is a high degree of sequence similarity and structural conservation of these genes and gene products, confirmation of the true identity and function of these neuropeptides remains ... effect of sinus gland extract and neuropeptide on vitellogenin gene expression in M japonicus In that study, the effect of a CHH peptide and two MIH-like peptides on ovary vitellogenin gene expression ... PC (2000) Clustering of mandibular organ-inhibiting hormone and moult-inhibiting hormone genes in the crab, Cancer pagurus, and implications for regulation of expression Gene 253, 197–207 Gu...
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Báo cáo khoa học: A (1fi3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of fibrinogen-like protein and epidermal growth factor gene expression pot
... SDEGFI-PREC gene (Fig 6) The level of expression of the housekeeping gene, tubulin, was not altered by the presence of curdlan In view of the above finding, that curdlan causes phosphorylation of Tyr ... macrophages and expression of those genes which are involved in inflammation [22] At present, our knowledge of sponge immune molecules, including cytokines and enzymes involved in blood coagulation and ... the expression level of the glucanbinding protein at the beginning of the experiments is low However, after only day of incubation in the presence of 10 lgÆmL)1 curdlan, a strong upregulation of...
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Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding pot
... determined The effects of the replacement of C74, C105 and C265 with a serine residue on the expression and complementation of the mutant phenotype of the gas1 null mutant of S cerevisiae were ... features of proteins of the GH72 family and demonstrate that the most N-terminal highly conserved cysteine is crucial for the folding and maturation of Gas1p Materials and methods Strains and growth ... presence of the His-tag (sGas1-H) did not appreciably modify the expression level of sGas1523 and sGas1482, suggesting no deleterious effects of the additional amino acids on Gas1p expression and...
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Role of C-Reactive Protein and Procalcitonin in Differentiation of Tuberculosis from Bacterial Community Acquired Pneumonia potx
... sensitivity of 90.0% and a specificity of 58.9%; at a cutoff value of 0.25 ng/mL, the PCT concentration had a sensitivity of 93.1% and a specificity of 59.6% (Fig 1, Table 2) CRP and PCT concentrations ... Clinical and laboratory characteristics of the patients Of the 87 patients who met the inclusion criteria, 57 had bacterial CAP and 30 had pulmonary TB The median age of the bacterial CAP and pulmonary ... PCT is a good predictor of the severity of pneumonia and sepsis, as described previously [31,32], and implies that PCT can be used effectively for site -of- care decisions and for predicting CAP...
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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
... presence of both DNA and bis-ANS decreased the stability of the protein (Fig 7B) Effects of high temperature on the secondary and tertiary structure of dlFG and sc-dlFG in the absence and presence of ... et al Fig Urea and guanidine treatment of dlFG and sc-dlFG in the presence of bis-ANS (A) Effects of increasing urea concentrations on the spectral center of mass of (m) dlFG and (r) sc-dlFG ... on stability of genetic fusion of the two subunits of the dlFG dimer using the variant named 2CTdlFG, which takes advantage of the physical proximity of the N-terminus and C-termius of the two...
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basic protein and peptide protocols
... wells at the very ends of the row of wells, for samples in these often suffer distortion during the electrophoresis 14 Be wary of the dangers of electric shock and of fire, and of the neurotoxic acrylamide ... faults and their remedies are given in Table Some examples are shown in Fig Be wary of the dangers of electric shock and of fire, and of the neutrotoxic acrylamide monomer Ready-made solutions of ... prepared by increasing %T and adjustment of %C Such adjustment of %T and %C may be found empirically to improve resolution of closely migrating species A combination of large and small pore gels,...
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protein and peptide analysis by mass spectrometry
... be capable of the analysis of large underivatized peptides (8), and, soon after, of proteins, by the demonstration of the first mass spectrum of insulin in 1982 (9) and of a number of snake toxms ... Separation and solvatton of analyte molecules m the solid phase, avoiding agglomeratron of analyte material, Uptake of laser energy used for desorptton and ronizatlon of matrix and analyte molecules, and ... the performance of lower flow columns ES and ion-spray iomzation offer methods for the mol-wt determination of protems and other biopolymers with very acceptable accuracy, and also offer convenient...
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Báo cáo sinh học: " Vaccinia virus A12L protein and its AG/A proteolysis play an important role in viral morphogenic transition potx
... of IV toward IMV by the failure of AG/A cleavage event Figure Morphology defects in the absence of A12L expression Morphology defects in the absence of A12L expression To investigate a role of ... synthetic promoter (pA12L) and AG/A mutant plasmid of A12L (AG/A), and infected with vvtetOA12L in the absence of Tet The transient expression of A12L induced regular IV and IMV particles (panel ... the presence and absence of Tet (30 µg/mL) and harvested at 3, 5, 8, 12, and 24 hpi Each virus titer (PFU/ ml) was scaled in log phase Morphology defects in the absence of A12L expression In...
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Báo cáo sinh học: "Alpha-1 antitrypsin protein and gene therapies decrease autoimmunity and delay arthritis development in mouse model" potx
... (DK58327) and University of Florida Office of Research Author details Department of Pharmaceutics, University of Florida, Gainesville, FL 32610, USA 2Department of Pathology, University of Florida, ... received saline injection ELISA for the Detection of Serum hAAT and BAFF Levels and Antibodies against hAAT, bCII and mCII Detection of hAAT and anti-hAAT antibodies in mouse serum was performed ... the effect of hAAT on autoantibody production, we evaluated the levels of anti-CII autoantibodies in total Ig, and IgG1 and IgG2a subclass at early (day 35) and late (day 49) stages of the disease...
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Báo cáo hóa học: " Vaccinia virus A12L protein and its AG/A proteolysis play an important role in viral morphogenic transition" pdf
... of IV toward IMV by the failure of AG/A cleavage event Figure Morphology defects in the absence of A12L expression Morphology defects in the absence of A12L expression To investigate a role of ... synthetic promoter (pA12L) and AG/A mutant plasmid of A12L (AG/A), and infected with vvtetOA12L in the absence of Tet The transient expression of A12L induced regular IV and IMV particles (panel ... the presence and absence of Tet (30 µg/mL) and harvested at 3, 5, 8, 12, and 24 hpi Each virus titer (PFU/ ml) was scaled in log phase Morphology defects in the absence of A12L expression In...
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Báo cáo hóa học: " Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions" pdf
... Schell J: The promoter of TL-DNA gene controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector Molecular and General Genetics 1986, 204:383-396 ... traditional concept of the gene, this notion of neatly delineated, modular genes has become increasingly blurred by the discovery of the myriad complex interactions governing gene expression and protein ... are often employed in attempts to identify the genetic basis of important aspects of a pathogen's phenotype For example, in order to understand the genomic determinants of pathogenicity, genetic...
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Statin Therapy, LDL Cholesterol, C-Reactive Protein, and Coronary Artery Disease pot
... important implications for understanding the pathogenesis of the progression of atherosclerosis and the mechanism of benefit of statin therapy The Pravastatin or Atorvastatin and Infection Therapy (PROVE ... presenting antigen and activating T cells, and their expression is often increased in inflammation Increased production of interferon-g by activated T cells is characteristic of a number of autoimmune ... evaluated a broad range of reductions in LDL cholesterol and CRP, permitting a post hoc analysis of the relationship between these two biomarkers and the rate of progression of atherosclerosis across...
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