animal models for in vivo percutaneous penetration studies
Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
Ngày tải lên :
07/03/2014, 21:20
... selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core sequence and maintained allergen-specific IgE-binding epitopes and the use of a Sel-tag enabled labelling ... inhalation In this study, we tracked inhaled Der p in vivo using the recently developed Sel-tag method in a mouse model for Der p 2-sensitization Based upon our findings we conclude that the in ammatory ... In vivo tracking of 75 Se-labelled Der p intrinsic biological properties of the allergens may all contribute to their allergenicity [1–4] The intrinsic properties required for evoking an...
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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc
Ngày tải lên :
17/03/2014, 10:20
... bacterial dsbB protein in the periplasm also contains two cysteine pairs acting in concert for the transfer of disulfides to dsbA [25] Interestingly, the redox function of dsbB is linked to the bacterial ... motif Therefore the systematic functional analysis of the six cysteines in yeast Erv1p was the major goal of this paper All three pairs of cysteines in Erv1p are indispensable for in vivo activity ... cysteines of yeast Erv1p can be grouped into three pairs (Fig 1): one pair near the N-terminus of the protein, the central pair close to the FAD binding motif and two cysteines in the C-terminal...
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Báo cáo hóa học: " Are quantum dots ready for in vivo imaging in human subjects?" docx
Ngày tải lên :
22/06/2014, 18:20
... eventually translate QDs for use in clinical applications such as in vivo imaging in human subjects Modeling studies have revealed that two spectral windows exist for QD imaging in living subjects, one ... types of QDs tested in these studies was excreted in the feces within 1–2 days In vivo targeted imaging using QDs In order to make QDs more useful for in vivo imaging and other biomedical applications, ... exhibited high affinity integrin avb3 specific binding in cell culture and ex vivo In vivo NIR fluorescence (NIRF) imaging was carried out on athymic nude mice bearing subcutaneous integrin avb3-positive...
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Báo cáo sinh học: " Prediction of breeding values with additive animal models for crosses from 2 populations" ppt
Ngày tải lên :
09/08/2014, 18:22
... vector containing the BV ofq animals for the traits, ordered by trait within animal Then, G is the 2q x 2q covariance matrix between traits and individuals Following Elzo (1990) the inverse of ... compute for a general situation, involving animals from several crossbred genotypes Lo (1993) presented an efficient algorithm for computing BLUP in the case of 2- and 3-breed terminal crossbreeding ... an incidence matrix that relates phantom parents to genetic groups Elements in each row of [P are all zero, except for two 1/2’s in the columns pertaining to :P] b the parents of the animals in...
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Báo cáo sinh học: "Inequality of maximum a posteriori estimators with equivalent sire and animal models for threshold traits" pps
Ngày tải lên :
09/08/2014, 18:22
... evaluation for discrete polygenic traits in animal breeding In: Advances in Statistical Methods for Genetic Improvement of Livestock (D Gianola, K Hammond, eds), Springer-Verlag, Berlin, 361-409 ... evaluation for type traits with linear and non-linear procedures Livest Prod Sci 15, 165-171 Knuiman MW, Laird NM (1990) Parameter estimation in variance component models for binary response data In: ... data In: Proc 5th World Congr Genet L PP A Livest Prod 22, 49-50 Mayer M (1994b) Numerical integration techniques for point and interval estimation of breeding values for threshold traits In: ...
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báo cáo khoa học: "BRCAA1 monoclonal antibody conjugated fluorescent magnetic nanoparticles for in vivo targeted magnetofluorescent imaging of gastric cancer" ppsx
Ngày tải lên :
11/08/2014, 00:23
... cancer of mm in diameter and IVIS imaging system and Magnetic Resonance Imaging, investigated the feasibility of as-prepared nanoprobes for non-invasive in vivo targeted dual modal imaging of gastric ... shown in Figure 8, after injecting the nanoprobes, a significant change in signal intensity was observed in some regions of tumors, indicating that there existed accumulation of the nanoprobes in ... used in all animal imaging experiments Nanoprobes for MR imaging of nude mice loaded with Gastric Cancer For MR imaging [33], gastric MGC-803 cells (1 × 10 ) were injected subcutaneously into...
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Báo cáo y học: " Identification of unique reciprocal and non reciprocal cross packaging relationships between HIV-1, HIV-2 and SIV reveals an efficient SIV/HIV-2 lentiviral vector system with highly favourable features for in vivo testing and clinical usa
Ngày tải lên :
13/08/2014, 09:21
... as a high affinity binding site for Gag The resulting RNAprotein complex is then targeted to the plasma membrane where virion budding takes place Poly A pol ∆ Env RRE Figure packaging constructs ... transfer to the brain of primates for treatment of experimentally induced Parkinson's disease [6] Packaging of unspliced vector mRNA in the producer cell line is a key part in process of lentiviral ... The dotted line indicates a deletion damaged areas of the brain Such cells have the potential to be useful for the treatment of Parkinson's disease, spinal cord injury and other inflammatory...
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Báo cáo y học: " A remission spectroscopy system for in vivo monitoring of hemoglobin oxygen saturation in murine hepatic sinusoids, in early systemic inflammation" doc
Ngày tải lên :
13/08/2014, 13:20
... reduction in hepatic sinusoidal HbsO2 during the early stages of systemic inflammation In parallel, we detected an increasing NAD(P)H autofluorescence representing an intracellular inadequate ... requires changing the continuous intravenous infusion rates of the dye to provide stable plasma concentrations With mice (increasingly used as laboratory animals) there is a growing need for a method ... represents a simple and reliable method for hepatic sinusoidal HbsO2 determination in small rodents In combination with NAD(P)H autofluorescence, it provides information on the oxygen distribution,...
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Báo cáo y học: "New animal models for evolution and development" pdf
Ngày tải lên :
14/08/2014, 14:21
... Tijana Copf in his lab had shown that cad inactivation by RNAi in the larva can abolish all trunk segments, depending on the time of the interfering RNA injection At the molecular level cad inactivation ... Washington University, Bellingham, USA) deletes almost the entire anterior end of the animal This indicates that in Nasonia, hb functions as a major player, and that the otx-hb interaction in ... with bilateral symmetry, including both insects and vertebrates) Averof also reported work of Tassos Pavlopoulos in his group who has succeeded in establishing transgenesis in the amphipod crustacean...
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Báo cáo khoa học: LRRK2 in Parkinson’s disease: in vivo models and approaches for understanding pathogenic roles pdf
Ngày tải lên :
23/03/2014, 04:20
... model systems, including lower eukaryotes and invertebrates, in order to obtain clues for building important hypotheses The rapidity and efficiency of in vivo studies in many nonmammal models have ... Z Yue In summary, these studies in fruit flies have provided important in vivo information regarding the potential function of LRRK2 (Table 1) Indeed, certain observations reported in fly models ... ‘contaminants’ in the process of searching binding proteins, the in vivo evidence for the relationship between ROCO proteins and cytoskeletons in D discoideum suggests a need to further investigate...
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Báo cáo y học: " In vivo imaging approaches in animal models of rheumatoid arthritis" pot
Ngày tải lên :
09/08/2014, 01:22
... rheumatologists interested in surgical procedures for in vivo imaging, there is a rich arsenal of procedures for imaging within almost all major organs of mice or rats Almost all were developed originally for ... yield surprising results and will be highly informative in the effort to cure arthritis Competing interests None declared Acknowledgements The author thanks his laboratory group for inspiring discussions ... EC, Constantin G: Molecular mechanisms involved in lymphocyte recruitment in inflamed brain microvessels: critical roles for P-selectin glycoprotein ligand1 and heterotrimeric G(i)-linked receptors...
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Báo cáo y học: " Gene therapy in animal models of rheumatoid arthritis: are we ready for the patients" pdf
Ngày tải lên :
09/08/2014, 01:24
... of proinflammatory cytokines, thereby reducing their circulating levels and abolishing a sensitizing effect on naïve joints A conflicting finding was the protection of the ipsilateral hind paw ... accessory protein-producing NIH-3T3 fibroblasts into knee joint inhibited murine CIA in this joint, but it did not protect the distal paws from developing CIA Soluble forms of IL-1RacP, in contrast ... rates in experimental arthritis Apart from local effects in the injected joint, it is becoming increasingly clear that local treatment also affects arthritis in nearby joints This is an intriguing...
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Báo cáo y học: "The role of the Notch pathway in healthy and osteoarthritic articular cartilage: from experimental models to ex vivo studies" pdf
Ngày tải lên :
12/08/2014, 15:22
... ligand-receptor interaction and a cysteine rich region The intracellular domain consists of ankyrin repeats, a glutamine-rich domain and a PEST (proline, glutamate, serine, threonine) domain [14,15] ... AM, Zecchini V, Brennan K: CSL-independant Notch signaling: a checkpoint in cell fate decisions during development? Curr Opin Gene Dev 2002, 12:524-533 19 Fortini M: Notch and presenilin: a proteolytic ... also in proliferation and apoptosis in developing and mature articular cartilage, recent studies have focused on the involvement of this pathway in joint pathology Several studies were interested...
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Tài liệu Báo cáo " Development of system of HydrodynamicEnvironmental models for coastal area (Case study in Quang Ninh - Hai Phong region) " doc
Ngày tải lên :
13/02/2014, 12:20
... through the water-sediment interface So we obtain a system of three models for the oil slick, oil in water column and in the bottom sediment environments Since these models are related to each ... Hai In this case, the oil slick is transformed into shape of an ellipse with centre in the oil slick origin In the case of NE wind, the oil slick is quickly approached Do Son - Hai Phong shoreline, ... oil concentration in sediment after (a) and 24 (b) hours in the NE wind Conclusion The preliminary results for the combined system of 2D, 3D hydrodynamicenvironmental models for SPM and bottom...
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Tài liệu Báo cáo khoa học: Animal models of amyloid-b-related pathologies in Alzheimer’s disease docx
Ngày tải lên :
16/02/2014, 09:20
... having sufficient power are therefore extremely important in minimizing the in uence of confounding factors arising from inherent problems associated with breeding APP transgenic mice and maintaining ... aggregation in sporadic AD brain There is now a renewed interest in studying APP processing in sporadic AD brain, and in understanding the mechanisms of Ab truncation and modification Most transgenic models ... understanding of AD pathogenesis Therapeutic studies in AD models Access to good animal models is crucial to success in developing disease-modifying therapeutics However, AD neuropathology is incomplete...
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Tài liệu Báo cáo khoa học: "You’ve Got Answers: Towards Personalized Models for Predicting Success in Community Question Answering" doc
Ngày tải lên :
20/02/2014, 09:20
... ”want in answer ”adenocarcinoma” in question ”was” in question ”live” in answer Q avg pos votes ”would” in answer ”answer” in question ”just” in answer ”me” in answer ”be” in answer in the” in ... 2000 Information retrieval on the web ACM Computing Surveys, 32(2) Y Liu, J Bian, and E Agichtein 2008 Predicting information seeker satisfaction in community question answering In Proceedings ... by information gain for ASP (trained for all askers) and ASP Group (trained for the group of askers with 20 to 29 questions) Conclusions We have presented preliminary results on personalizing...
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Tài liệu Báo cáo khoa học: "Phrase-Based Backoff Models for Machine Translation of Highly Inflected Languages" docx
Ngày tải lên :
22/02/2014, 02:20
... models for combining in- domain and 42 out-of-domain data for the purpose of bootstrapping a part-of-speech tagger for Chinese, outperforming standard methods such as EM i i i Backoff Models in ... significant differences to the baseline model are shown in boldface (p < 0.05) Set train1 train2 train3 train4 train5 Set train1 train2 train3 train4 train5 German-English baseline backoff BLEU PER BLEU ... the baseline system An analysis of the backoff system output showed that in some cases (e.g examples C and 45 Set train1 train2 train3 train4 train5 Set Set train1 train2 train3 train4 train5 German-English...
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Báo cáo khoa học: Measuring enzyme activities under standardized in vivo-like conditions for systems biology pdf
Ngày tải lên :
06/03/2014, 09:22
... enzyme of interest should be investigated in the context of the in vivo- like medium Finally, in vivo, the enzymes are present at much higher concentrations than in typical enzyme assays, in which ... at least three independent cell-free extracts from steady-state samples from a single chemostat culture Enzyme In vivo- like Vmax (mmolÆmin)1Æg protein)1) Flux (mmolÆmin)1Æg protein)1) HXK PGI ... determined above Vmax values are key paremeters of kinetic models of metabolic processes (see, for examples of kinetic models, [38–43] and the website JWS Online Cellular Systems Modelling [44];...
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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
Ngày tải lên :
07/03/2014, 00:20
... function of the increasingly large group of proteins that have been shown to contain intrinsically unstructured regions The intrinsic conformation flexibility that is inherent in this binding mechanism ... Protein A Agarose ⁄ Salmon Sperm DNA incubation for no more than h Beads were washed twice for 15 in lysis buffer containing 150 mm NaCl, followed by a 15-min wash in lysis buffer containing 500 ... min), in strain YPP310 (light grey bars, DDABDswi1 + snf5) lacking both activator-binding domains (SWI1D329-655, SNF5D2-327), strain YPP211 (white bars, DABDsnf5) lacking the Snf5 activator-binding...
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Báo cáo khoa học: A simple in vivo assay for measuring the efficiency of gene length-dependent processes in yeast mRNA biogenesis doc
Ngày tải lên :
07/03/2014, 12:20
... Kokubo T (2001) Mutations in the TATA-binding protein, affecting transcriptional activation, show synthetic lethality with the TAF145 gene lacking the TAF N-terminal domain in Saccharomyces cerevisiae ... biogenesis complex, involved in mRNP formation, show the lowest GLAM ratios measured in this work This is not the case in other RNA processing mutants that have been analyzed in this work In agreement ... timeconsuming and not easy to carry out in a high number of samples In addition, the information obtained by nuclear run-on shows the location of active polymerases, but it gives no information...
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