animal and in vitro model studies future uses of dhea
Báo cáo y học: "Data from necropsy studies and in vitro tissue studies lead to a model for allometric scaling of basal metabolic rate" pot
... brain and the epithelial tissues of the skin and gastrointestinal tract, the midpoint of the values of bi is 0.71 The midpoint of the values of ki is -0.14, the midpoint of the values of scaling ... values of βi This midpoint is estimated as the midpoint of the values of ki plus the midpoint of the values of bi because values of ki are not available for certain tissues For scaling of the brain ... family of scaling models with an unspecified number of parameters Because the number of degrees of freedom is undefined, it is not possible to make a standard comparison of the goodness of fit of...
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Báo cáo y học: "In vitro model for the analysis of synovial fibroblast-mediated degradation of intact cartilage" pptx
... Neidhart M, Gay RE, Gay S: Anti-interleukin-1 and anti-CD44 interventions producing significant inhibition of cartilage destruction in an in vitro model of cartilage invasion by rheumatoid arthritis ... metalloproteinases in the catabolism of the interglobular domain of aggrecan in vitro Biochem J 1999, 344(Pt 1):61-68 Patwari P, Gao G, Lee JH, Grodzinsky AJ, Sandy JD: Analysis of ADAMTS4 and MT4-MMP indicates ... types in an experimental setting which reflects prominent features of joint destruction in RA In the long run, the system may allow the testing/screening of the molecular basis and efficacy of new...
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Báo cáo y học: "CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model" pdf
... regulates integrin-mediated events are being elucidated, and studies suggest that CD47/IAP ligation may lead to modulation of integrin conformation and affinity state or influence integrin-mediated ... Immunoprecipitation of α5 integrin and β1 integrin and subsequent Western blotting showed co-precipitation of CD47/IAP with α5 integrin precipitates only (results not shown) Roles for CD47/IAP in regulation of ... modulate integrin affinity without intracellular signalling has been suggested [31] The extracellular IgV domain of CD47/IAP may interact with the integrin, inducing change to a high-affinity state...
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Báo cáo y học: "Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children" pptx
... doses of LPS and Pokeweed Mitogen lectin as stimulants in vitro LPS-induced signalling in macrophages, and in other LPS-responsive cells such as neutrophils, is known to be initiated by interaction ... release of multiple inflammatory and antiinflammatory mediators [24,25] Recently a new model of LPS interaction has been proposed including a signalling complex of receptors, formed following LPS ... quantita- Page of tive and qualitative differences in the cytokine responses that could explain gender differences in the inflammatory profile Higher levels of cytokines in males could be explained by...
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Báo cáo y học: "Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children" pot
... doses of LPS and Pokeweed Mitogen lectin as stimulants in vitro LPS-induced signalling in macrophages, and in other LPS-responsive cells such as neutrophils, is known to be initiated by interaction ... release of multiple inflammatory and antiinflammatory mediators [24,25] Recently a new model of LPS interaction has been proposed including a signalling complex of receptors, formed following LPS ... quantita- Page of tive and qualitative differences in the cytokine responses that could explain gender differences in the inflammatory profile Higher levels of cytokines in males could be explained by...
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Pathobiological studies of zoonotic blastocystis subtypes using in vitro model systems
... disorder of gastrointestinal tract presenting with abdominal pain and altered bowel habits Compared to healthy controls, a higher incidence of Blastocystis in IBS patients is reported in several studies ... reaching intestine transform into the vacuolar form and undergo binary fission Following reproduction, the vacuolar forms encyst again and are passed out in the feces (Tan, 2004) Molecular typing ... Several intestinal and non-intestinal symptoms are associated with Blastocystis infections 1.6.1 Intestinal symptoms Blastocystosis frequently presents with diarrhea and abdominal pain (Tan,...
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In vivo and in vitro studies of th17 response to specific immunotherapy in house dust mite induced allergic rhinitis patients
... treatment were used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen ... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation IL10 producing CD4+ T cells showed a distinct ... markers in each study group tested in vitro 3.2 In vivo Study 3.3.1 Th17 specific gene mRNA/protein expression Following a 3-day in vitro stimulation, there were mRNA/protein levels of Th17 associated...
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Analysis, sequencing and in vitro expression of PCR products
... that represent protein domains are expressed and functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique ... separation in thin-coated capillaries containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and ... exhausted the primer in excess continues to allow linear accumulation of one strand of DNA suitable for subsequent DNA sequencing Analysis, sequencing and in vitro expression of PCR products 99...
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Tài liệu Báo cáo khoa học: Trophoblast-like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins pdf
... Binding studies Binding studies of human HDL3 to choriocarcinoma cells were performed at °C in medium (F12K/DMEM without FBS) with increasing amounts of 125I-labeled HDL3 (1, 5, 10, 25, 50, and ... inactivated fetal bovine serum (FBS, hyClone, Utah, USA) containing 100 UÆmL)1 penicillin/streptomycin and mM L-glutamine at 37 °C under 5% CO2 The JAr and Jeg3 cell lines (ATCC) were maintained ... [31] using bovine serum albumin as a standard Oxidation of LDL (ox-LDL) was performed with 1.66 lM of Cu2+ in NaCl/Pi as described [32] Lipoprotein labeling procedures Single-labeling (a) Iodination...
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Báo cáo Y học: A Ca2+/CaM-dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter ppt
... kinases are involved in stress signaling pathways [29] and recent work points towards the involvement of Ca2+/CaM-dependent protein kinases in stress signaling [16], changes in the level of PsCCaMK ... an in vitro substrate for the PsCCaMK, the effect of phosphorylation on its DNAbinding property was tested The protein was in vitro phosphorylated using cold ATP and used for DNA binding studies ... Protein fractions eluted from kinase antibody affinity column containing lg purified p40 (fraction A) and those containing lg of both p40 and p72 PsCCaMK kinase (fraction B) were used for in vitro...
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báo cáo hóa học: " A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices" potx
... present antigen and are responsible for the production and release of a variety of cytokines and chemokines, interact with immune cells and are intimately involved in immunoregulation within the CNS ... according to the Council of Europe (Directive 86/609) and Finnish guidelines, and approved by the State Provincial Office of Eastern Finland Exposure of slices to different stimuli A series of experiments ... this model Conclusion In this report, we used inflammation as a model to illustrate the efficacy of in vitro hippocampal slice culture as an intermediate model between single cell lines and in...
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báo cáo hóa học: " Beyond platinum: synthesis, characterization, and in vitro toxicity of Cu(II)-releasing polymer nanoparticles for potential use as a drug delivery vector" ppt
... containing no Cu (left) The difference between the amount released at 48 h and that contained in the original loading solution determined Cu loading, resulting in values ranging between 12 and ... synthesis, and metal loading RP conceived of the study, and participated in its design and coordination, carried out in vitro toxicity studies, SEM and EDX analysis and drafted the manuscript Competing ... Particle size of approximately 215 nm was determined via dynamic light scattering (DLS) ICP MS Cu-loading studies For Cu-loading studies, the Cu-loading solution containing CuSO4 and nanoparticles...
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Báo cáo lâm nghiệp: "Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations" pptx
... auxins, it promoted early rooting and increased the final rooting percent- age and the number of roots per explant [21, 22] Putrescine is involved in root induction by increasing the activity of ... the use of putrescine, which in combination with low concentration of auxin, in rooting medium, was essential in stimulating high rooting percentage with high quality of roots, resulting in fast ... reducing vitrification problems and aimed at improving shoot length and rooting percentage, by modifying the medium composition in terms of salt balance, type of gelling agent, by adding pectin of...
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Báo cáo khoa học: " Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos" pps
... gniniatnoc pord muidem noitalupinam a otni derrefsnart dna ,muidem noitalupinam hserf ni eciwt dehsaw ,)24333 tshceoH lm/gµ gniniatnoc ASB-HLT eerf-muiclac( muidem noitalupinam ni nim 51 rof ... ,h rof edimixeholcyc lm /gµ 01 gniniatnoc muidem CVI ni detabucni erew setycoo detalumits-ortcelE 2lCgM Mm 50.0 dna 2lCaC Mm 10.0 gniniatnoc lotinnam Mm 082 ni ces yb detarapes cesµ 06 rof V ... )v/w( %4.0 gniniatnoc muidem 32 -)USCN( ytisrevinU etatS aniloraC htroN saw tnempoleved oyrbme rof muidem )CVI( erutluc ehT stnemirepxe lla ni desu saw hctab emas eht morf diulf ralucillof enicroP...
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Báo cáo y học: "PTPN22 polymorphism and anti-cyclic citrullinated peptide antibodies in combination strongly predicts future onset of rheumatoid arthritis and has a specificity of 100% for the disease" ppsx
... having any of them As none of the controls had this combination, the OR was calculated assuming one individual did, resulting in a value of 132.03, although the relative risk was actually infinite ... negative regulation of T-cell signalling, as demonstrated in an animal model [17] and in human cell lines [5] The functional effect of the PTPN22 1858 polymorphism on T-cells in humans is yet to ... showed an increased number of germinal centres and increased immunoglobulin levels, although autoantibodies were not detected in these animals Changes in B-cell function were not found, suggesting...
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Báo cáo y học: "yclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ – prostaglandin E2 dependent proliferation of growth plate chondrocytes" ppsx
... chondrocytes in vitro and in situ Protein expression of COX-1 and COX-2 was studied using isoform-specific antibodies Both COX isoforms could be detected in all zones of the growth plate In cultured ... results obtained in studies investigating the effects of PGE2 This indicates the crucial role played by species and culture conditions used in the various in vitro systems According to our in vivo ... analysis and interpretation of data and was involved in drafting the manuscript CJK was involved in data interpretation, drafting the manuscript and revised it critically for the physiological and...
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báo cáo khoa học: " A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas in vivo and in vitro" pptx
... existence of VM in GBC by using immunohistochemistry staining, TEM and micro-MRA technology in vitro and in vivo, respectively All authors have read and approved the final manuscript 19 Competing interests ... participate in vessel-like structures formation in vitro, and succeeded in establishing GBC-SD and SGC-996 nude mouse xenograft models In addition, Page of 12 highly invasive GBC-SD cells when grown in ... staining: 12 paraffin-embedded tissue specimens of tumor xenografts were deparaffinized, hydrated, and stained with H&E Companion serial section were stained with double staining of CD31 and...
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Báo cáo y học: "Phenotype and in vitro function of mature MDDC generated from cryopreserved PBMC of cancer patients are equivalent to those from healthy donors" ppt
... repertoire of DC matured in the presence of inflammatory stimuli comprises pro-inflammatory cytokines and chemokines, including the T cell inhibitory cytokine IL-10, the Th-1 promoting cytokine IL-12, ... Mixtures of peptides consisting of 15 amino acid residues, overlapping by 11 amino acids each, were designed to span the sequences of CMV pp65, CEA, MAGE-3, and the intracellular domain (ICD) of HER-2/neu ... PerCP-Cy5.5 and CD4 APC and acquired on a flow cytometer CD3+CD4+ lymphocytes were gated including the blasts and excluding CD209+ MDDC The percentage of cells showing decreased CFSE staining intensity...
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Báo cáo y học: " Cysteinyl-leukotrienes in the regulation of β2-adrenoceptor function: an in vitro model of asthma" ppsx
... of the experiments, was involved in HASMC culture, performed in vitro cAMP studies and helped in the manuscript preparation LB participated in the design of the experiments and was involved in ... no competing interests SC declare no competing interests LB declare no competing interests SR declare no competing interests MM declare no competing interests EC declare no competing interests ... concentrations inhibiting 50% of active force in Salbutamol concentrations inhibiting 50% of active force in carbachol-contracted human bronchial rings Effects of montelukast on salbutamol concentrations inhibiting...
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Báo cáo khoa học: "In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair" ppt
... Differences in the manufacturing according to initial concentrations of carbon sources, surface/volume ratios, strain of Acetobacter and extended times of fermentation interfere in the final product ... Committee at the School of Veterinary Medicine and Animal Science, São Paulo State University Before surgery, anesthesia was induced by intramuscular injection of a combination of xylazine 2%, 10 mg/kg ... of inflammatory and immune response, means of disintegration and its longevity in the host tissue [1] The inflammatory response may delay the healing process [23] In the present study a low inflammatory...
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