anfinsen apos s thermodynamic principle and the proteomic code
Báo cáo y học: "The Proteomic Code: a molecular recognition code for proteins" docx
Ngày tải lên :
13/08/2014, 16:21
... complementary bases at the right positions of these subsequences However, this might be the case only because the first and last codons form simpler subsequences and contain longer repeats of the same nucleotide ... content were also selected to ensure a wide range of structural representation Linear regression analyses and Student 's t-tests were used for statistical analyses of the results Observations were made ... [GC-NSF(antisense)] and from SNS repeating sequences [(SNS)n] similar to the GC-NSF(antisense) The similarity between GNC/SNS-type primitive codons (which are expressed even from the reverse-complement...
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Báo cáo khoa học: A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins docx
Ngày tải lên :
30/03/2014, 04:20
... possesses two intradisulfide bonds between Cys12–Cys45 and Cys88–Cys298 These disulfide bonds are required for assembly into hexamers and for the structural stability of the protein [17–19] Thus, ... proteins are synthesized and are translocated to the ER during the maturation stage of embryogenesis Previously, we demonstrated that the synthesis of glycinin was initiated when the seeds achieved ... repeats and the G-box) of napA gene in Brassica napus; Required for seed specific expression Soybean consensus sequence found in the 5’-upstream region of b-conglycinin gene Soybean consensus sequence...
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Báo cáo hóa học: "Reference values and physiological characterization of a specific isolated pig kidney perfusion model" doc
Ngày tải lên :
20/06/2014, 00:20
... represents the water reabsorption along the tubular system which is numerically present in the second scale of the y-axis in figure Statistics All assessed data are expressed as mean ± standard ... catheter in calibrated glass cylinders Perfusion system The perfusion system consisted of separated blood and dialysis circuits as described [2], that may also be used for the perfusion of other ... This situation is sometimes discussed as "decoupled" Because there is more than normal oxygen per unit sodium consumed, the generation of heat shock proteins (HSPs) is proposed [52] as one reason...
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Báo cáo hóa học: " Design and Characterization of a 5.2 GHz/2.4 GHz ΣΔ Fractional-N Frequency Synthesizer for Low-Phase Noise Performance" potx
Ngày tải lên :
22/06/2014, 22:20
... fabrication The measured integrated phase noise of the WLAN synthesizer was 0.5◦ rms for the lower band and 0.535◦ rms for the upper band and that is close to the predicted phase noise These results are ... plot as the cross-over point between these two curves at an offset frequency of 200 kHz Therefore the best possible out-of-band phase noise is the raw phase noise of the VCO and the inband phase ... capacitors and one resistor For offchip filters, the loss experienced by capacitors is negligible Thus, the loop filter contains only one noise source, the thermal noise associated with the resistor R The...
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Báo cáo khoa học: A strategy for the generation of specific human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx
Ngày tải lên :
23/03/2014, 13:20
... nucleotide sequences were compared with the databases using the BLAST algorithm The best scores corresponded to human immunoglobulins The nucleotide sequences were also compared with the IMGT databases ... Interestingly, clones selected from the standard procedure had fewer changes (usually one), whereas using the stringent strategy, the selected clones showed 2–6 changes Clone 6009F was selected and ... cells The clones selected with this procedure were evaluated by ELISA as soluble proteins Expression of single-chain antibodies The scFv inserts from the selected clones, were ligated into the...
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Báo cáo hóa học: " Nucleotide mismatches between the VP7 gene and the primer are associated with genotyping failure of a specific lineage from G1 rotavirus strains" potx
Ngày tải lên :
20/06/2014, 01:20
... rotavirus strains in phylogenetic lineages, as it was showed by Santos et al [7] in G9 strains when they used the Gouvea 's RTPCR strategy 15 Authors' contributions 17 GIP performed the sequences analysis, ... v3.65 softwares The statistical significance of the tree was preformed by bootstrapping, using 1000 pseudo-replicates data sets All the strains grouped within one of the four lineages previously ... the sequences analysis, phylogenetic analysis and wrote the manuscript; EEE download the sequences from database, performed the sequences analysis and critically revised the manuscript 14 16 18...
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Báo cáo y học: "Validation of a specific measure to assess healthrelated quality of life in patients with schizophrenia and bipolar disorder: the ‘Tolerability and quality of life’ (TOOL) questionnaire" doc
Ngày tải lên :
09/08/2014, 01:21
... in these multidimensional scales we used the unweighted scores In both cases, the higher the scores, the better the HRQoL, and vice versa (2) The Spanish version of the TOOL questionnaire The ... of severity as the generalised UKU scale Despite these results, this study presents some limitations that should be considered A ceiling effect was observed in two items: stiffness-tremor and ... and several adverse events frequently associated with antipsychotic treatment (fatigue-weakness, weight gain, stiffness-tremor, physical restlessness, sexual dysfunction, and dizziness-nausea)...
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Báo cáo y học: "Association of a specific haplotype across the genes MMP1 and MMP3 with radiographic joint destruction in rheumatoid arthritis" ppt
Ngày tải lên :
09/08/2014, 01:23
... components of the immune system, such as the SE and the cytokine network [1,2,30] In the cohort investigated here, the association of the SE with radiographic progression was seen, as it has in previous ... between the two groups was made by the Ratingen score, which had to exceed 24 after years in the group with severe disease The hypothesis that a joint haplotype across the genes MMP3 and MMP1 shows ... (severity of disease as measured by the Ratingen score) was used as a quantitative measure (Ratingen score) and analysed by factorial regression All P values from regression analyses were also checked...
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Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx
Ngày tải lên :
09/08/2014, 10:20
... lining with some cells interspersed in the deeper tissues (Figure 4a,b) Staining in the perivascular regions of the small vessels is also noted (Figure 4c) In these cases, the staining appears to be ... microtome The slides were then rehydrated in descending graded ethanol and processed through the same immunostaining steps as for frozen sections Results Mass spectrometric analysis of two-dimensional ... differences in protein synthesis by FLSs in health and disease Previously, proteomic studies were initiated to determine the protein composition and expression patterns of FLSs [4] One of the proteins...
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báo cáo khoa học: "Design and characterization of protein-quercetin bioactive nanoparticles" doc
Ngày tải lên :
11/08/2014, 00:23
... Intensity increase in the intensity of fluorescence, as well as a blue shift, was observed when the concentration of DMSO in the BSA and Lys systems was less than 70% (Figures 13A and A’); this ... spectroscopy were combined to study the secondary and tertiary structures of the protein aggregates Results and Discussion Size and Zeta Potential Measurements Scanning transmission electron microscopy ... determine the hydrodynamic particle sizes of the system [24,25] The size of D-BSA (Figures 3A and 3A’) and D-Lys (Figures 4A and 4A’) was less than 50 nm when the concentration of DMSO was less than...
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Design and characterization of functional novel oligopeptides
Ngày tải lên :
04/10/2015, 10:25
... deprotection is also possible In this study, the oligopeptides were synthesized using the Pioneer peptide synthesizer from the Applied Biosystems The solid-state peptide synthesis principle using the Fmoc ... and other structures [3] There are many potential applications for self-assembled oligopeptides such as model systems for understanding the diseases like Alzheimer s disease, scaffold in tissue ... fold and refold proteins [20] The same is also true for other protein systems, involved in biomineralization processes responsible for the formation of hard tissues such as bones, teeth and seashells...
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Design and characterization of interposers for high speed fine pitch wafer level packaged device testing
Ngày tải lên :
04/10/2015, 10:25
... the series resistance of the conductors The series resistance a signal sees in propagating along the signal and return paths is related to the conductors’ bulk resistivity and the cross section ... characterized One of proposed interposers is a MEMS based interposer using silicon as the substrate and the other is an elastomer interposer The MEMS based interposer has special MEMS contacts to provide ... circuits in order to establish measurements results and demonstrate functional test performance The measurement results will serve as a good comparison to the simulations results as well The test specifications...
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Tài liệu Báo cáo khoa học: Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier pdf
Ngày tải lên :
21/02/2014, 03:20
... pastoris and screening for ASP3c expression The expression plasmid was linearized with BglII and transferred into the Pichia pastoris yeast host by the electroporation method as described in the ... strain DH5a was used for DNA subcloning and propagation of the recombinant plasmid Pichia pastoris strain GS115 (his4) was used in the expression study Oligonucleotides were synthesized by MGW ... This suggests a general similar global fold for insect CSPs, which would be composed of six a-helices as observed in the X-ray structure of a CSP from M brassicae [32] Like many CSPs from S gregaria...
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Báo cáo khoa học: Characterization of the role of a trimeric protein phosphatase complex in recovery from cisplatin-induced versus noncrosslinking DNA damage potx
Ngày tải lên :
30/03/2014, 04:20
... the yeast strains and drug sensitivity studies were as described previously [9] The strains used in this study are listed in Table The S cerevisiae strain AY925, in which Psy4p bears a C-terminal ... pph3D, psy4D and psy2D mutant cells with cisplatin causes enhanced accumulation of cells in S- phase as compared with WT cells, although the effect is less conclusive in the case of the psy4D cells ... pph3D, psy4D and psy2D cells, and separated by PFGE These gels resolved a characteristic ladder of bands corresponding to the 16 S cerevisiae chromosomes, visualized after ethidium bromide staining...
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Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx
Ngày tải lên :
30/03/2014, 20:20
... chromosomes (Fig 9, panels and 7) Some of the ISP36 did not diffuse into the cytoplasm and remained near the chromosomes These results suggest that ISP36 may still be a constituent of some macro-structure ... mouse C3H cells A plasmid harboring GFP-tagged ISP36 cDNA was transfected into mouse C3H cells and the GFP–ISP36 protein was transiently expressed The cells were immobilized, and the DNA was stained ... corresponds to that in Fig 1B ‘UniProt Accession no.’ shows the accession number in the Universal Protein Resource Database ‘Molecular mass’ values were obtained from the amino acid sequences S ...
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Knowledge transfer in Tehran University of Medical Sciences: an academic example of a developing country pdf
Ngày tải lên :
11/08/2014, 05:21
... 0.02 Sex (male/female) Associate professor (in comparison to an assistant professor) Professor (in comparison to an assistant professor) Instructor (in comparison to an assistant professor) Non-academic ... topic based on other organizations' request or needs assessment increases the score significantly According to the results of the linear regression analysis in table 4, health system research and ... interests Authors' contributions SN and RM participated in the design, statistical analysis, and manuscript writing JG designed and conducted the study SN, MG, MS, and MA gathered the data KM assisted...
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báo cáo khoa học: " Knowledge transfer in Tehran University of Medical Sciences: an academic example of a developing country" pot
Ngày tải lên :
11/08/2014, 16:21
... 0.02 Sex (male/female) Associate professor (in comparison to an assistant professor) Professor (in comparison to an assistant professor) Instructor (in comparison to an assistant professor) Non-academic ... topic based on other organizations' request or needs assessment increases the score significantly According to the results of the linear regression analysis in table 4, health system research and ... interests Authors' contributions SN and RM participated in the design, statistical analysis, and manuscript writing JG designed and conducted the study SN, MG, MS, and MA gathered the data KM assisted...
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Magnetic resonance spectroscopy correlation with histological analysis in gliomas and structure determination of a hypothetical protein
Ngày tải lên :
10/11/2015, 11:35
... HRMAS 1HMRS is that it s rapid, nondestructive and requires only small amounts of unprocessed samples Unlike chemical extraction or other forms of tissue processing, this method analyzes tissue directly, ... susceptibility-induced line-shape distortions, the presence of broad uncharacterized resonances from macromolecules, lipids and unsuppressed water, and low signal-to-noise ratios The analysis of such data is greatly ... Aliphatic side chain assignment 3.2.2 Secondary structure 3.2.2.1 Chemical Shift Index 3.2.2.2 NOE analysis 3.2.3 NOE assignments 3.2.4 Structural statistics 3.3 Discussion 3.3.1 Sequential assignment...
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Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
Ngày tải lên :
16/03/2014, 16:20
... electrospray mass spectroscopy The measured mass of PfPDO was 25 648 ± 0.5 Da The measured mass of C3 5S and C14 6S was 25 628 ± 0.5 Da, and that of C3 5S/ C14 6S was 25 613 ± 0.4 Da Thus, the difference ... cysteine was Cys146, as this was observed at the lowest ratio of iodoacetate to iodoacetamide This was followed by Cys149, Cys35, and Cys38, which was the last to react and the least accessible ... filtration and in the presence of the cross-linking agent DMS In all the conditions tested, the presence of the dimer was never observed It was observed only in the presence of the cross-linking...
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