andrea damascelli probing the electronic structure of complex systems by arpes

Ngày tải lên: 11/06/2014, 10:40

... studied the effect of the mutations on the thermodynamic and kinetic stability. The similarity of the impact on both the thermodynamic and kinetic stabilities suggests a predominant effect on the ... stability is caused by destabilization of the native state relative to th e unfolded state. The decrease in the thermodynamic s tability of the other less s table v ariants, all of which were exclusively obtained ... F start is the signal of the substrate before the addition of enzyme, F end is the signal after cleavage of all substrate, and [E] is the concentration of RNase A . CD spectroscopy CD spectra of RNase...

Ngày tải lên: 16/03/2014, 18:20

... present for the last four residues at the C-terminus. The stereochemistry of the model has been assessed using the program procheck [23]. Ninety per cent of the residues fall in the most favoured regions ... metal- bound hydroxyl group onto the substrate, as observed in other members of the amidohydrolase superfamily [24,25]. However, as no structure of complexes with either the substrate, product or inhibitors ... content of 50%. Data were pro- cessed using the program mosflm [38], and the ccp 4 suite of programs [39] was used for scaling. Structure deter- mination for hACMSD was performed by means of the...

Ngày tải lên: 18/02/2014, 06:20

... A ˚ resolution by the molecular replacement method. The schematic view of the overall structure is shown in Fig. 3A. Theenzymeexistsasadimeroftheabc heterotrimer. There is a noncrystallographic twofold ... cob(II)alamin by X-ray irradiation [59]. Therefore, we believe that the structure reported in this paper is also that of the glycerol dehydrataseÆcob(II)alamin complex. The dihedral angle of the northern ... from a bulk of water by the corrin ring of cobalamin. Figure 4B shows the structure around the enzyme-bound cobalamin. The cobalamin molecule is bound between the a and b subunits in the so-called...

Ngày tải lên: 21/02/2014, 03:20

Ngày tải lên: 17/03/2014, 03:20

... the form curl/π (2) + E (2) . 5. Strategy of the proof of the Asymptotics Theorem In this section we describe the main ideas in the proof of the Asymptotics Theorem. (1) Section 6. We start by ... given a detailed proof of the theorem by relying heav- ily on a result, which we have called the Asymptotics Theorem, concerning the geometric properties of the causal structure of appropriately ... I + 0 is the future domain of the origin on Σ 0 . Remark 9.3. The proof of Corollary 9.2 requires an extension argument. The estimates of the Asymptotics Theorem, which are uniform with respect to the...

Ngày tải lên: 16/02/2014, 05:20

... to the enzyme. This may indicate that the steric limitation of the enzyme’s binding pocket takes place in the area between the ortho and para positions of the aromatic substrate. On the basis of ... removes the steric hindrance and allows the approach of 2IPA towards the catalytic iron. Residue I248 lies at the entrance of the substrate- binding pocket of the enzyme, leading to the substrate channel. ... [14]. The lack of characterization of the structural determinant of the substrate specificity of the AtdA enzyme has thus limited its development as a biocatalyst for the bioremediation of a wide...

Ngày tải lên: 19/02/2014, 02:20

... best IM:Type full time IM:Title the godfather Other the, in, that Other the, in, that Other the, in, that Table 3: Label sets and their respective query segment examples for the intent class Movie, Job ... goal of this work is to identify the semantic labels of IMs, i.e., the attribute names they implicitly refer to. We use A c to denote the set of IM semantic labels for the intent class c. Other Additionally, ... name of an intent class. For example, the IH of the query “alice in won- derland 2010 cast” is “cast”, which is an attribute name of Movie. By issuing the query, the user in- tends to find out the...

Ngày tải lên: 20/02/2014, 04:20

... A ˚ , respectively, for the backbone of the two subunits. The difference between rmsd of monomeric and dimeric species is due to the indetermination of the reciprocal orientation of the two subunits. The average ... was used to back calculate the NOESY cross-peaks from the calculated structure to assess the quality of the structure. The final family was made up of 30 structures with the lowest target function. ... is c onsidered. The c omparison of the present structure with the X -ray structures of the human oxidized protein ( 1SOS) [11] and its G 37R mutant [50] show that the protein has the same folding...

Ngày tải lên: 21/02/2014, 03:20

... to the active site. To dissect the structure function relationships of the LPL carboxyl- terminal domain, several residues predicted by the model structure to be essential for the functions of ... modeling of the dimeric structure of LPL. Two dimeric model structures of LPL were constructed using INSIGHT II version 2000 on a Silicon Graphics workstation. The model structure of the closed ... on the crystal structures of the human, porcine, and horse PLs (Fig. 1). A frontal view illustrates the overall saddle shape of the dimeric structure and the key heparin-binding residues in the...

Ngày tải lên: 21/02/2014, 15:20

... secure the services of eminent members of appropriate professions in the examination of policy matters pertaining to the health of the public. The Institute acts under the responsibility given to the ... 20418 NOTICE: The project that is the subject of this report was approved by the Governing Board of the National Research Council, whose members are drawn from the councils of the National Academy of Sciences, ... recognizes the superior achievements of engineers. Dr. Wm. A. Wulf is president of the National Academy of Engineering. The Institute of Medicine was established in 1970 by the National Academy of Sciences to...

Ngày tải lên: 06/03/2014, 15:20

... ª 2004 FEBS the full-length HOP, they act to facilitate the assembly of multichaperone regulatory complexes. The struc- tural independence of these TPR domains, and the presence of independent ... First, the only structures of natural TPRs in the PDB (13 TPR-containing coordi- nate sets) consist of nonglobular, extended arrays of helices [9,10,13–21]. Second, the majority of these (11 structures) ... TPR1 and TPR2A of AB DC Fig. 2. Solubility of NlpI constructs and the structure of mature NlpI. (A) 10–20% gradient SDS ⁄ PAGE of NlpI expression products, showing the insolubility of the 3-TPR construct...

Ngày tải lên: 07/03/2014, 16:20

... another than to any other profile in the data set. The profiles of G can be summarized by a vector s whose dimensionality is that of the data, and each of whose elements contains the mean of ... disparity in the frequency of its occurrence in A and B is the first significant finding of the proposed methodology. The scaling of the ‘Allah’ variable dominates all the other variables. ... understanding their thematic interrelationships the object of this exercise—but it is not sufficient because it provides no information about the thematic characteristics of the clusters and the thematic...

Ngày tải lên: 08/03/2014, 04:22

... [27]. On the basis of the Ping Pong mechanism, the acetylation rate (k 2 )of NAT2 by the acetyl donor is independent of the trans- acetylation rate (k 4 ) of the arylamine substrate. Because the values ... the active site. The more dras- tic mutations, Y190I and Y190A, however, revealed the importance of the phenyl ring of the Tyr side chain on the pH dependence of enzyme acetylation. Both of the ... trunca- tions, the availability of the Tyr190 mutants makes it feasible to conduct cell-based studies of the effects of the stability of the acetylated enzyme intermediate on the N-acetylation of aromatic...

Ngày tải lên: 16/03/2014, 00:20

... orientation. Accordingly, the present data on the molecular mass of the native Hb complex as well as the Hb subunits are inconsistent. In addition, there are only two hypo- thetical models concerning the structure of ... identification of the carbohydrates bound to D. magna Hb subunits, saccharides were released by methanolysis and separated by GC followed by MS. Chromatograms of the carbohydrates of D. magna Hb, of the ... of this interesting molecule. The present study has contributed to this approach: (a) the determination of the molecular mass of the native Hb complex and its subunits as well as the number of...

Ngày tải lên: 16/03/2014, 13:20

... reduce output by restricting the use of the property, and if so this is as much a cost of the property right as the cost of the fence is. Suppose the owner of a shop- ping center does charge for the use of ... for the original. In the case of books and other printed matter, the copier may not be able to match the quality of paper or binding of the original or the crispness of the printing, and there ... numerous, the trans- action costs of their buying the right to use the river from the factory may ex- ceed the value of the right to them. In such a case a liability rule would be better, whereby the...

Ngày tải lên: 16/03/2014, 15:20

... both by the orientation of the energy transfer partners and by the distance between them [28]. The fact that the efficiency of transfer varies with the 6th-power of the distance between the fluorophores presents ... is not promoted by the bivalent nature of the antibodies. In studies assessing the effect of ligand-binding on the quaternary structure of the receptors, the possibility that the antibodies could ... in 50% of the maximal efficacy (R 0 ). As indicated by the Fo ¨ rster equation, the efficiency of transfer varies inversely with the 6th-power of the distance. The extreme steepness of the relationship...

Ngày tải lên: 16/03/2014, 22:20

Ngày tải lên: 17/03/2014, 00:20

Ngày tải lên: 17/03/2014, 08:20

Ngày tải lên: 17/03/2014, 23:20