and tor regulate multiple processes in eukaryotic cells

... number of cells was analyzed (Fig 2B) In order to investigate whether CEdG has the potential to accumulate in genomic DNA of nongrowing cells, HSC-T6 cells were kept in minimal medium containing 1.5% ... CEdG was introduced into the luciferase reporter gene vector pGL3 Control CEdG-modified and unmodified vectors were transfected in parallel into HEK 293 T cells, and gene expression was investigated ... shown in Table The amount of CEdG formed during the DHA incubation of the vector pGL3 Control increased with both incubation time and quantity of glycating reagent The reaction mixture containing...

Ngày tải lên: 30/03/2014, 04:20

... are down-regulated both in vitro and in vivo Moreover, Hp induces genomic instability in nuclear CA repeats in mice and in mtDNA of AGS cells and chronic gastritis tissue, and this effect in mtDNA ... with continuous shaking E coli were maintained in the LB liquid medium (containing 100 µg/ml ampicillin) at 37°C for 12 h with continuous shaking Preparation of Hp and E.coli extract The Hp and E ... Medicine 57 Reagents F12 culture medium (Hangzhou Jino Biology Co., Ltd China), fetal bovine serum (FBS), ampicillin, penicillin and streptomycin (Shanghai Bio-engineering Co., Ltd China), brain...

Ngày tải lên: 25/10/2012, 11:18

... Insulin is a polypeptide hormone that regulates glucose, lipid and protein metabolism and promotes cell growth and differentiation On ligand binding, the insulin receptor tyrosine kinase initiates ... cells were pretreated with actinomycin D (AD) followed by insulin (Fig 2A,C) Basal levels of HO-1 protein were reduced following AD treatment, and the HO-1 protein and mRNA response to insulin ... (CHX) was administered to establish the role of protein synthesis in insulin-induced HO-1 expression (Fig 2B,C) CHX abrogated HO-1 protein induction following insulin treatment but, in agreement...

Ngày tải lên: 07/03/2014, 12:20

... galectin 3, an S-type lectin containing a carbohydrate-binding domain, as a physiological ligand of CD98 in vivo [18] Figure 5A shows the primary intracellular distribution of galectin in the ... and in intestine [26]) Finally, there is evidence for galectin as a factor in RNA splicing, based on the localization of the protein in the nucleus [27] In this article, we further examine to ... CD98 and galectin membrane trafficking P Dalton et al and formation of activated complexes with other proteins, in particular b1-integrin, galectin and CD147, has been proposed by various investigators...

Ngày tải lên: 23/03/2014, 09:20

... Meijer, L., Shaw, G & Strnad, M (1997) Cytokinin-derived cyclin-dependent kinase inhibitors: synthesis and cdc2 inhibitory activity of olomoucine and related compounds J Med Chem 40, 408–412 ... and b), Cdk5 and p25 (Fig 2B, c and d, respectively) in the transfected cells To determine whether CIP inhibits the protein phosphorylation activity of Cdk5/p25 in transfected cells, kinase activity ... alpha helices in p25 as determined by Tarricone et al [21] The sequences comprising p25, p16 and CIP are indicated by the labelled arrows (B) A combination of N-terminal and C-terminal truncations...

Ngày tải lên: 23/03/2014, 21:21

... involved in EGF-like factor and Tace/Adam17 expression in cumulus cells, we examined the effect of PKA inhibitor (H89), p38 MAPK inhibitor (SB203580), PI3K inhibitor (LY294002) and MEK inhibitor (U0126) ... expressed in porcine granulosa cells in vivo in response to hCG administration [12] Thus, in vivo during the ovulation process, LH induces EGF-like factor expression in granulosa cells and the ... factors, Amphiregulin (AREG), Epiregulin (EREG) and β-cellulin (BTC) were also recently reported as potent factor The EGF-like factor was induced by LH stimuli in granulusa cells, and EGF receptor...

Ngày tải lên: 20/06/2014, 07:20

... not defined Institute Information Project Name Investigation of rice kernel cracking and its control in the field and during post-harvest processes in the Mekong Delta of Vietnam Vietnamese Institution ... millers and extension workers aware of various factors responsible for harvesting and milling losses and degradation of rice quality To increase the research and teaching capability of institution and ... also undertaken in Can Tho and Long An provinces Cracking behaviour of the grain due to threshing was also investigated in Can Tho and Kien Giang provinces Data of the actual harvesting losses due...

Ngày tải lên: 21/06/2014, 06:20

... tour Information was gathered from those institutes with regard to rice drying, post-harvest handling, farmers’ training and milling Dr Bhandari and Prof Shu Fukai (Australian cooridinators) ... participated in the training session The harvesting and drying demonstrations and results obtained in the previous research were presented during these sessions Dr Vinh Truong, Mr Tran van Khanh, and ... losses during spring/dry harvesting season was also undertaken in Kien Giang, Can Tho and Long An provinces Cracking behaviour of the grain due to threshing was also investigated in Can Tho and Kien...

Ngày tải lên: 21/06/2014, 06:20

... Province Project: INVESTIGATION OF RICE KERNEL CRACKING AND ITS CONTROL IN THE FIELD AND DURING POST-HARVEST PROCESSES IN THE MEKONG DELTA OF VIETNAM Figure 1: Opening by Dr Truong Vinh, CARD 026/VIE05 ... combine-harvester at Tan Phat A co-operative, after training lesson Nong Lam University Ho Chi Minh city CARD Project 026/VIE05 Report on training program in July-2007 At Kien Giang Province ... Figure 1: Opening by Dr Truong Vinh, CARD 026/VIE05 project coordinator Training program 02/2007 Place: Meeting hall of people’s committee of Tân Hiệp district...

Ngày tải lên: 21/06/2014, 06:20

... KERNEL CRACKING AND ITS CONTROL IN THE FIELD AND DURING POST-HARVEST PROCESSES IN THE MEKONG DELTA OF VIETNAM Figure 1: Opening by Dr Truong Vinh, CARD 026/VIE05 project coordinator Training program ... after training lesson Nong Lam University Ho Chi Minh city CARD Project 026/VIE05 Report on training program on 29-30 Sept-2007 At Can Tho Province (Vinh Thanh & Thot Not Districts) Project: INVESTIGATION ... after training lesson Figure 8b: Discussion on the drying performance at Tan Thoi co-operative, after training lesson Figure 9: Visit the reaper in field at Tan Thoi co-operative, after training lesson...

Ngày tải lên: 21/06/2014, 06:20

... operation Losses during crop and grain handling: • Handling loss: mature grain lost during lifting, hauling, stacking, pouring, and bagging of crop and grains Incorrect time of harvesting increases harvest ... rats, and handling Lodging loss: plants with mature grains fall on the ground making the grains difficult to recover Standing crop loss: standing plants with mature grains are left standing in the ... combined collection-threshing Cutting by reaper Combined collection and harvesting Cutting and laying the crop on a windrow is done using a reaper, followed by collection and threshing using...

Ngày tải lên: 21/06/2014, 06:20

... first drying pass (drying temperature used 73, 75, 80, 85, 87 oC), drying time for pass was 2.5 mins, and then tempered in incubator for 40 mins Drying temperature and drying time for pass and low ... gelatinization, the enhancement of kernel integrity with respect to hardness and stiffness during tempering, were identified in this study These findings assisted in improving our understanding ... multilevel factorial design, comprising two drying temperatures (80 and 90 oC), two drying durations (2.5 and 3.0 min), and four tempering durations (0, 30, 40, and 60 min), was used in the experiment...

Ngày tải lên: 21/06/2014, 06:20

... harvesting, reaping, threshing, sun/mechanical drying, loading/unloading, transporting, milling processing and storage conditions Milling processing is an important stage as it produces the final product ... MILLING SYSTEM INTRODUCTION There are many factors accounting for the post harvesting losses of rice and occurring as early as pre-harvesting stage and subsequent periods from harvesting to storage ... the main causes leading to low rice recovery in a small scale factory By proper awareness, training of operators and maintance of mills the head rice recovery can be substantially improved In Tien...

Ngày tải lên: 21/06/2014, 06:20

... drying and sun drying According to report of Cuong (2003) (presented at the seminar on post-harvest handling in Can Tho dated 28/4/2003), field drying increased grain cracking by 10% resulting in ... LongAn (Bu Tin, VN 95-20) Harvesting method Notation Hand and heaped immediately Hand and dried in the sun (one day) Reaper and heaped immediately Reaper and dried in the sun (one day) Hand and heaped ... summation of handling losses and value losses For instance, improper harvesting resulted in high shattering (handling losses) and low head rice recovery (value losses) Similarly, sun drying in bad weather...

Ngày tải lên: 21/06/2014, 06:20

... to be trained in the project II THE TRAINING DOCUMENT The training documents were updated and improved time by time with more information in the later training series They are shown in the Appendices ... all into English) Nong Lam University Ho Chi Minh city CARD Project 026/VIE05 FARMERS AND EXTENSION WORKERS TRAINING 2007 Summary of the training activities and documents used in the training ... 1 Institute Information Project Name Investigation of rice kernel cracking and its control in the field and during post-harvest processes in the Mekong Delta of Vietnam Vietnamese Institution...

Ngày tải lên: 21/06/2014, 06:20

... 1 Institute Information Project Name Investigation of rice kernel cracking and its control in the field and during post-harvest processes in the Mekong Delta of Vietnam Vietnamese Institution ... temperature fluidised bed drying (using two rice varieties) Experimental results on flat bed drying including survey results on the drying costs of various drying methods practiced in Mekong delta ... temperature compact dryers and flat-bed driers (year results) The report is divided into sections: Experimental results on harvesting time and methods in two seasons (rainy 2006 and dry 2007) High temperature...

Ngày tải lên: 21/06/2014, 06:20

... Changes in cracking behaviour and milling quality due to post-drying annealing and subsequent storage This study investigated the effect of drying temperatures, tempering regimes and storage conditions ... dried in multi-pass mode has being undertaken at Nong Lam University Training activities to disseminate the information on harvesting and drying practices, and demonstrations of dryers and combine ... making the kernels then being strong enough to withstand breakage during subsequent milling This study also enhanced the understanding of rice ageing during storage in relation to changes in...

Ngày tải lên: 21/06/2014, 06:20

... conducted during two years (2006-2008), introducing various milling lines and equipments, and evaluating the investment efficiency, industrialization planning of milling system in MRD (Figures ... first drying pass (drying temperature used 73, 75, 80, 85, 87 oC), drying time for pass was 2.5 mins, and then tempered in incubator for 40 mins Drying temperature and drying time for pass and low ... about the interesting information received during the training to friends or neighbours who did not participate in the training In addition, farmers also play an active role in self-learning to...

Ngày tải lên: 21/06/2014, 06:20

... -15b and let-7f were downregulated in effector cells compare to naïve cells and increased back in memory T cells [14] Effector T cells originate from naïve T cells following antigen exposure and ... role in innate and acquired immune response Elucidating the mechanisms by which miR-155 expression is controlled and finding its targets will bring more understanding of the mechanisms of tumor induction ... miR-150, is mainly expressed in the lymph nodes and spleen and is highly up-regulated during the differentiation of mature T and B cells suggesting that it may participate in B and/ or T lymphopoiesis...

Ngày tải lên: 08/08/2014, 17:20

... HNF4α binding than FOXA2-3 (Table and Figure 5a-c, e-g) In addition, FOXA12-3 binding is highly correlated with HNF4α binding in HepG2 cells (Table and Figure 5d, h) Allele-specific DNA-protein interactions ... genes in the liver Therefore, we examined the binding pattern at the FOXA genes and compared this with our data on upstream stimulatory factor (USF)1 and USF2 [26], and HNF4α and GABP (GA binding ... detect and verify possible co-bindings and to further understand whether these are due to binding of different FOXA molecules at the same site in different cells or due to co-binding in the same...

Ngày tải lên: 09/08/2014, 20:21