and sp d lungs infected with s pneumoniae

... NAC Statistical analysis The data are expressed as mean ± SEM Statistical analysis was performed with Student 's t test for paired samples following analysis of variance Differences were considered ... concentrations, CSC has been shown to cause cell death, which seems to be due to high concentrations of oxidants and other radicals [27] and this study) Moderate oxidative stress induces apoptosis, whereas ... freshly prepared CSC (C) Next, BrdU was added and the cells were incubated for another h and subsequently washed and fixed BrdU incorporation was detected by immunocytochemistry (for details see...

Ngày tải lên: 12/08/2014, 18:20

... processes [Discher, 2005] 2.3 Stiffness of substrate Solid substrates used in many research for the adhesion of cells can range in stiffness from soft to rigid, and also vary in topography and thickness ... thickness Regardless of geometry, the stiffness of a substrate is given by its intrinsic resistance to a stress applied This is measured by the substrate s elastic modulus E, which is obtained by ... uniform and sustained contractile prestress from the edge to near the nucleus [Disher, 2005] Stress versus strain illustrated for several soft tissues extended by a force (per cross-sectional...

Ngày tải lên: 28/09/2015, 13:28

... biological studies and experiments and wrote the manuscript MP performed biological experiments OR synthesized the dendrimers used in this study COT designed and synthesized the dendrimers JJF designed ... experiment JPM supervised chemical achievements AMC designed dendrimers and supervised chemical achievements RP designed and supervised biological studies, coordinated the study and wrote the paper ... anti-CD3/ CD28 stimulation of PBMCs or purified CD4+ T cells, 5.104 CFSE labelled cells were mixed 1.2.103 anti-CD3/ CD28 mAb-coated Dynabeads (Invitrogen) and displayed in U-shaped 96 well plates...

Ngày tải lên: 18/06/2014, 15:20

... (PBS) were added to each well and incubated for hours at 37°C The medium was removed and formazan was dissolved in DMSO and the optical density was measured at 570 nm using a Bio-assay reader ... the dead animals, the predominant lesion was hemorrhagic gastroenterititis [3] Food additives are substances added to food to preserve flavor or improve its taste and appearance Food additives ... Medicine and Toxicology 2009, 4:27 animals Chunks of borax were used to control in the pH+ in drilling muds Cattle consumed these chunks They appeared depressed, were dehydrated and some had diarrhea...

Ngày tải lên: 20/06/2014, 00:20

... fusion procedures It has been anecdotal that, despite good surgery, the results are not always satisfactory This is possibly due to altered bone healing as a result of the disease process and/ or ... participated in the design of the study, performed the statistical analysis and helped to draft the manuscript PWL & NM conceived of the study, and participated in its coordination BA designed the study, ... it is assumed that cells of each donor would lose their identity and all cells despite their origin would behave consistently in a similar manner to various agents Our study suggests this probably...

Ngày tải lên: 20/06/2014, 01:20

... fluorescence microscopy (Olympus IX71, Japan) Statistics Data were expressed as the mean ± SD (standard deviation) from at least three independent experiments Onetailed unpaired Student s t-test ... electronic sensing (RT-CES) assay also provided the dynamic process and binding behavior between cells and related agents Experimental Reagents and Materials Three different-sized ZnO nanoparticles capped ... Student s t-test was used for significance testing, and P \ 0.05 is considered significant Results and Discussion Cytotoxicity of Different-Sized ZnO Nanoparticles for SMMC7721 Cancer Cells The Effect...

Ngày tải lên: 21/06/2014, 18:20

... triplicate All data were expressed as mean ± SD and analysed with SPSS 17 Comparisons for groups were made using Student s T-test One-way analysis of variance (ANOVA) with SNK or LSD test was used for ... was conducted after cells were harvested with trypsin and resuspended (8.0 × 105 cells/ml) in serum-free growth medium ASMC derived from asthmatic rats were divided into groups (control and intervention) ... 4°C), and the cell pellet was resuspended in ml saline To perform the differential leukocyte cell count, 0.1 ml of the cell suspension was drop on a glass slide and stained with Wright-Giemsa stain...

Ngày tải lên: 11/08/2014, 03:20

... triplicate All data were expressed as mean ± SD and analysed with SPSS 17 Comparisons for groups were made using Student s T-test One-way analysis of variance (ANOVA) with SNK or LSD test was used for ... was conducted after cells were harvested with trypsin and resuspended (8.0 × 105 cells/ml) in serum-free growth medium ASMC derived from asthmatic rats were divided into groups (control and intervention) ... 4°C), and the cell pellet was resuspended in ml saline To perform the differential leukocyte cell count, 0.1 ml of the cell suspension was drop on a glass slide and stained with Wright-Giemsa stain...

Ngày tải lên: 11/08/2014, 06:23

... were washed with PBS 2% FCS, stained with the indicated antibodies for 30 minutes and washed twice with PBS 2% FCS For the biotin-conjugated antibody, a second staining step with streptavidin conjugated ... CA, USA) For differentiation, MSCs were plated in six-well plates and grown to confluence Osteogenesis and adipogenesis were induced as described previously [29] and [30], respectively) Anti-CD3-induced ... medium and phenotyped (a -d) MSCs were incubated with the indicated antibodies and analyzed by flow cytometry Grey histograms show stained cells, and black lines represent cells incubated with isotype...

Ngày tải lên: 12/08/2014, 11:23

... increased, whereas BPS significantly decreased ratios of S plus G2/M and G2/M phases Histograms are representative and bar graph shows data expressed as means ± SE (n = 4) Open bars, ratios of ... transfected cells and confirmed target gene silencing by p27kip1 siRNA using Western blotting Statistical analysis The results are expressed as means ± SE Statistical analysis was performed using ... which has a random sequence, did not affect p27kip1 protein expression We found that BPS increased p27kip1 protein expression and significantly suppressed DNA synthesis in cells transfected with...

Ngày tải lên: 12/08/2014, 15:21

... design of the study FP conceived of the study with RLM, and participated in its design and coordination RLM conceived of the study, and participated in its design and coordination and statistical ... antigen-induced lymphoproliferative responses in cord blood with repeat measures in later childhood, and assessed the relative strengths of their associations with childhood asthma or eczema In addition, ... study with RLM, and participated in its design and coordination, its statistical analysis and helped to draft the manuscript KGK participated in the conduction and coordination of the study and...

Ngày tải lên: 13/08/2014, 13:22

... most exciting questions in the field addresses the issues of what failsafe processes limit Myc-induced proliferation and transformation and which mutations disable such failsafe mechanisms during ... missing A related issue is whether Cdk2 activation is required for Myc-induced apoptosis and there are models suggesting that suppression of p21Cip1 by Myc favors Myc-dependent apoptosis and ... however, several Myc-induced tumors are genomically unstable and show complex karyotypes and enhanced rates of double-strand breaks, which suggests that deregulated expression of Myc can also promote...

Ngày tải lên: 25/10/2013, 21:20

... a substrate for caspases and 7; Ac-YVAD-AMC is a substrate for caspase 1; Ac-IETD-AMC is a substrate for caspase and 10; Ac-LEHD-AMC is a substrate for caspases 2, 4, and Ac-DVPD-AMC, Ac-DPSD-AMC ... preferred substrate (bar A) for each was as follows: caspase 1, WEHD-AMC; caspase 2, VDVADAMC; caspase 3, DEVD-AMC; caspase 4, WEHD-AMC; caspase 5, WEHD-AMC, caspase 6, VEID-AMC; caspase 7, DEVD-AMC; ... fluorescent units and values for a DPSD-cleavage assay were 1165 relative fluorescent units Caspases and inhibitors Caspase substrates and their inhibitors were purchased from Biomol Ac-DEVD-AMC is...

Ngày tải lên: 19/02/2014, 13:20

... cycles) Individual results were normalized to GAPDH mRNA detected in each sample and expressed as a ratio to GAPDH DISCUSSION The process of angiogenesis involves the formation of new blood vessels ... analysis of phospho ERK1/2 immunodensity expressed as percentage of control (individual densities from ERK1 and bands were added up to generate one single ERK1/2 value) serum-cultured ECV304 cells ... *, significantly different from fetal bovine serum synthesis in cells cultured under standard conditions This effect was evident after 24 h of treatment and was still consistent in cells exposed...

Ngày tải lên: 21/02/2014, 01:21

... rates are also found in sall4-disrupted, klf5-disrupted, HDAC1-disrupted, ronin-disrupted and dicer-disrupted ES cells relative to wild-type ES cells, and mice with knockout of these genes also show ... apoptosis assay, and AP staining For the proliferation assay, · 105 cells were seeded in growth medium and counted every day over days of culture Viable and total cells were counted with and without ... cells was hybridized with radiolabeled cDNA probes Five micrograms of total RNA was loaded onto each lane (E) SSEA-1 expression in wild-type and Ptb) ⁄ ) ES cells ES cells were cultured on a feeder...

Ngày tải lên: 07/03/2014, 00:20

... P Srivastava and D Panda Fig Rotenone suppressed the reassembly of spindle microtubules in HeLa cells Cells were fixed at different time intervals Microtubules (red) and DNA (blue) were stained ... spindle microtubules assembled fast and formed normal spindles (Fig 4) In the presence of rotenone (0.2 lm and lm), microtubule reassembly was slow and spindles were not observed, even after 15 ... then added to the reaction mixtures and incubated for an additional 60 at 37 °C and the fluorescence spectra were recorded The excitation and emission wavelengths were 360 nm and 430 nm, respectively...

Ngày tải lên: 07/03/2014, 05:20

... experiments, 50 mm NaH2PO4 (pH 8.0) containing mm EDTA and ⁄ or dimethylsulfoxide was added to the culture Statistical analysis An unpaired Student s t-test was used for statistical analysis of the ... mitogenesis is the phosphatidylinositol 3-kinase (PI3K) ⁄ Akt pathway It is also an important mediator of cell growth and survival in response to growth factors and other signals PI3K activates Akt serine ... cartducin was prepared as described [7] Anti-mouse cartducin (also called CORS26) serum was purchased from R &D Systems (Minneapolis, MN) Anti(ERK1 ⁄ 2), anti-(JNK1 ⁄ 2), and anti-(p38 MAPK) sera...

Ngày tải lên: 16/03/2014, 13:20

... and methods at the specified times (A) Microsomal (membrane) and cytosolic (cytosol) fractions of AngIIstimulated SMC were assayed for arg-mART activity as described in Materials and methods with ... RNA was extracted and c-fos mRNA levels assessed by RTPCR as described in Materials and methods Molecular mass markers (DNA Marker VI) were used to confirm the size of the PCR products RNA loading ... TLC plate is shown, with PtdInsP1, PtdInsP2 and PtdInsP3 indicated These results were confirmed in two independent experiments (E) Quiescent SMC were treated for 15 with PGE2 (1 lM) subsequent to...

Ngày tải lên: 17/03/2014, 09:20

... expression is spatially regulated during postnatal development in chicken RNF13 is abundantly expressed in skeletal muscle tissue at early stages of embryonic myogenesis but its expression gradually ... development Ubiquitin ligases play critical roles in cancer development and the well-studied enzymes are MDM2 and SCFSkp2 [23] Elevated expression of MDM2 is apparent in various tumors and is ... myoblasts and its expression gradually decreases during skeletal myogenesis Interestingly, our work has also demonstrated that RNF13 expression is upregulated by the muscle growth inhibitor myostatin...

Ngày tải lên: 22/03/2014, 17:20

... wild-type and mutant strains, obtained by using a poly(U)-direct cell-free translation system, as described in the Materials and methods Strain Fig Cell-free protein synthesis using 14 mouse cDNAs ... proteins In the present study, seven streptomycin-resistant mutants and two streptomycin-dependent mutants of E coli were isolated, and were tested in our cell-free translation system We found one S1 2 ... used S3 0 extracts in cell-free CAT protein synthesis (Fig 2) The plasmid pK7-CAT was used as a standard template, and the components in the reaction mixtures were described in the Materials and...

Ngày tải lên: 30/03/2014, 13:20