and radiosensitization through inhibition of pi3k akt signaling
Apoptosis, Senescence, and Cancer docx
Ngày tải lên :
29/06/2014, 06:20
... Cabot 17 Chemo- and Radiosensitization Through Inhibition of PI3K/ Akt Signaling 313 Debra L Smith, Laura K Nolden, Gordon B Mills, and Yiling Lu 18 The Advancement of Epidermal Growth ... Cancer Institute, and Department of Medicine, Brigham and Women’s Hospital, Departments of Pathology and Medicine, Harvard Medical School, Boston, MA, and Broad Institute of Harvard and MIT, Cambridge, ... «Alexander Fleming», Institute of Molecular Biology and Genetics, Vari, Greece Andrzej Skladanowski • Laboratory of Molecular and Cellular Pharmacology, Department of Pharmaceutical Technology and...
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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf
Ngày tải lên :
07/03/2014, 03:20
... LY294002 (10 lm) or Akt siRNA (0.2 lg), and exhibited downregulation of P-gp at both the mRNA and protein levels (Fig 4A) Inhibition of the PI3K/ Akt pathway decreased the expression of P-gp induced ... results indicate that inhibition of the PI3K/ Akt signaling pathway may lead to inhibition of the MDR induced by PrPC in gastric cancer cells The mechanism underlying PI3K/ Akt- mediated PrPC-induced ... PrP-induced MDR in gastric cancer cells Inhibition of the PI3K/ Akt signaling pathway by LY2940002 or Akt siRNA leads to inhibition of PrPCinduced cell drug resistance and P-gp upregulation in gastric...
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Báo cáo y học: "Two specific drugs, BMS-345541 and purvalanol A induce apoptosis of HTLV-1 infected cells through inhibition of the NF-kappaB and cell cycle pathways" ppsx
Ngày tải lên :
10/08/2014, 05:21
... 14 15 Figure Effect of BMS-345541 on inhibition of IκB and p65 phosphorylation in vivo Effect of BMS-345541 on inhibition of IκB and p65 phosphorylation in vivo MT-2, C8166, and CEM cells were ... 52, and Flavopiridol showed less growth inhibition in inhibiting two out of four infected cell lines Consequently, we decided to focus and study the mechanism of BMS-345541 and Purvalanol A inhibition ... of Tax and an essential component in Tax-mediated NF-κB signaling in both canonical and non-canonical pathways Therefore, we reasoned that the specific targeting of both the NF-κB signaling and...
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Báo cáo khoa học: Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN ppt
Ngày tải lên :
06/03/2014, 00:21
... S6 and the GST-tag (C) Immunoblot analysis of the phosphorylation status of p70S6K and S6 in Paskin+ ⁄ + and Paskin) ⁄ ) MEFs (D) Immunoblot analysis of the phosphorylation status of p70S6K and ... phosphorylation of a microarray of 1176 peptides in the presence of [c-33P]ATP The magnified inset shows an example of the results obtained after detection by phosphorimaging (B) Peptide sequences of the ... Ser ⁄ Thr kinase (PSK)1 and PSK2, the budding yeast homologues of PASKIN, phosphorylate three translation factors and two enzymes involved in the regulation of glycogen and trehalose synthesis,...
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Báo cáo hóa học: " Synergistic Effect of Functionalized Nickel Nanoparticles and Quercetin on Inhibition of the SMMC-7721 Cells Proliferation" potx
Ngày tải lên :
22/06/2014, 00:20
... to the change of the cell membrane and formation of holes (arrows) on the surface of the relevant cell into cancer cells and increase the concentration of drug in cancer cells and thus could ... lmol/L of quercetin solution and cells incubated for h; d 50 lmol/L of quercetin solution and cells exposed to Ni nanoparticles cocultured for h and e 50 lmol/L of quercetin solution and cells ... results of electrochemical study of the amount of quercetin residues outside SMMC-7721 Fig Inverted fluorescence micrographs of SMMC-7721 cells after incubation with a 5.0 lmol/L of quercetin, and...
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Báo cáo y học: "Glucosamine affects intracellular signalling through inhibition of mitogen-activated protein kinase phosphorylation in human chondrocytes" pps
Ngày tải lên :
09/08/2014, 10:21
... collagenases MMP-1 and MMP-13 and the stromelysin MMP-3 Pretreatment with 2.5 and 10 mmol/l glucosamine was able, to differing degrees, to downregulate mRNA and protein levels of MMP-1, MMP-3 and MMP-13 ... reported by Chan and coworkers [34], who did not identify upregulation of MMP-2 and MMP-9 in bovine chondrocytes stimulated with IL-1β, and with those reported by Duerr [35] and Soder [36] and their ... classical markers of inflammation and cartilage degradation in OA joints It achieved this by affecting phosphorylation of JNK and p38 MAPKs and consequently inhibiting c-jun and junD activation...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
Ngày tải lên :
21/02/2014, 01:21
... Briefly, 90 lL of nuclei suspension were added with 100 lL of · reaction buffer (10 mM Tris/HCl, pH 7.5, mM MgCl2, 0.3M KCl, mM dithiothreitol, mM each of ATP, GTP, and CTP), and lL of [a-32P]UTP ... upper part of each panel shows representative autoradiograms corresponding to the immunoreactivity of ERK1/2 and phospho ERK1/2 (A,B), and MEK1/2 and phospho MEK1/2 (C,D) The lower part of each ... part of Panel A shows representative ethidium bromide stained gels of the reaction products obtained using lL of the RT products after 30 cycles of PCR amplification The lower part of panel A, and...
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Báo cáo sinh học: "Inhibition of mitotic kinase Aurora suppresses Akt-1 activation and induces apoptotic cell death in all-trans retinoid acid-resistant acute promyelocytic leukemia cells" ppt
Ngày tải lên :
18/06/2014, 19:20
... Bad and forkhead transcription factors [39] It has been reported that high expression of Akt is relative with survival, proliferation of leukemic cells in AML and inhibition of activation of Akt ... suppression of cell growth [40,41] In the present study, phosphorylation of Akt- 1 and GSK3b, the downstream of Akt- 1, was decreased in VX-680 treated NB4-R2 cells In addition, we also found that Akt signaling ... down-regulation of Akt- 1 phosphorylation at the activation site, Ser473 and decreased the level of phosphorylated GSK-3b at Ser9, the downstream of Akt- 1 (Figure 7B) Thus, VX-680 Xu et al Journal of Translational...
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báo cáo hóa học: " Pharmacological inhibition of Akt and downstream pathways modulates the expression of COX-2 and mPGES-1 in activated microglia" potx
Ngày tải lên :
19/06/2014, 22:20
... cyclooxygenase (COX)-2 and microsomal prostaglandin E synthase-1 (mPGES-1) and to the production of prostaglandin (PG) E2 and PGD2 Findings We show that inhibition of Akt by the Akt inhibitor X enhanced ... we demonstrate that blockade of mTOR and/ or PI3K/ Akt enhances prostanoid production and that PI3K/ Akt, GSK-3 and mTOR differently regulate the expression of mPGES-1 and COX-2 in activated primary ... pleckstrin homology domain of one of the Akt (also known as protein kinase B) isoforms and facilitates the phosphorylation of Akt1 , Akt2 or Akt3 at Thr308/309/305 and Ser273/474/472, respectively,...
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báo cáo hóa học: " Inhibition of gap junctional Intercellular communication in WB-F344 rat liver epithelial cells by triphenyltin chloride through MAPK and PI3-kinase pathways" pdf
Ngày tải lên :
20/06/2014, 00:20
... MAPK and PI3K However, both PD58059 and LY294002 abolished com- pletely the effect of TPTC downregulation of Cx43, implicating both MAPK and PI3K signaling cascades in a common mechanism of Cx ... TPTC, the P0 band disappeared, and a shift to bands of higher molecular weight occurred (P1) (Fig 6) Effects of TPTC on connexin 43 in immunofluorescence staining The expression of Cx43 in WB-F344 ... modulates Cx expression predominantly through activation of MAPK and PI3K signaling pathways Several in vivo and in vitro studies have revealed potential effects of organotins in broad spectrum including...
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báo cáo hóa học:" Inhibition of mitotic kinase Aurora suppresses Akt-1 activation and induces apoptotic cell death in all-trans retinoid acid-resistant acute promyelocytic leukemia cells" ppt
Ngày tải lên :
20/06/2014, 03:20
... Bad and forkhead transcription factors [39] It has been reported that high expression of Akt is relative with survival, proliferation of leukemic cells in AML and inhibition of activation of Akt ... suppression of cell growth [40,41] In the present study, phosphorylation of Akt- 1 and GSK3b, the downstream of Akt- 1, was decreased in VX-680 treated NB4-R2 cells In addition, we also found that Akt signaling ... down-regulation of Akt- 1 phosphorylation at the activation site, Ser473 and decreased the level of phosphorylated GSK-3b at Ser9, the downstream of Akt- 1 (Figure 7B) Thus, VX-680 Xu et al Journal of Translational...
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Báo cáo y học: "Resistance to IL-10 inhibition of interferon gamma production and expression of suppressor of cytokine signaling 1 in CD4+ T cells from patients with rheumatoid arthritis" ppsx
Ngày tải lên :
09/08/2014, 01:24
... in sustaining the activation of macrophages and synovial fibroblasts through cell surface signaling by means of cell surface CD69 and CD11, as well as the release of proinflammatory Th1 R568 cytokines ... presence of IL-10, the induction of STAT1 and STAT3 phosphorylation by IL-10, and the expression of SOCS1 and SOCS3 mRNA in peripheral blood (PB) CD4+ T cells from RA patients Materials and methods ... defective IL-10 inhibition of CD28-costimulated production of both IFN-γ and IL-2 Numerous cytokines, both proinflammatory and anti-inflammatory, have been detected in the ST of RA, and the balance...
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Báo cáo khoa học: "Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo" docx
Ngày tải lên :
09/08/2014, 09:20
... antibody of DNA-PKcs However, it is necessary to expand this kind of study, especially to verify the efficacy and mechanisms of the radiosensitization of this kind of scFv molecules through the ... detection of Gy-induced DNA-PKcs activity using the Signa-TECT® DNA-Dependent Protein Kinase Assay System Immunoblotting pattern (C) and relative p -Akt level (the ratio of p -Akt/ Akt signals) (D) of Akt, ... excitation wavelength of 485 nm and an emission wavelength of 538 nm Data were expressed as arbitrary units of fluorescence per microgram of protein (F/mg protein) The average level of F/mg protein...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
Ngày tải lên :
10/08/2014, 05:21
... of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater effects on Akt ... mechanism of pancreatic islets FN and LAM enhances activation of Akt and ERK The ECM bind to cells by activating signaling molecules such as Akt and ERK Therefore, we analyzed the effect of FN and ... Page of 10 FN and LAM-induced enhancement of IPC differentiation depends on Akt and ERK activation To examine the involvement of Akt and ERK activation in enhancing IPC differentiation by FN and...
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Báo cáo y học: "Knockdown of Rab5a expression decreases cancer cell motility and invasion through integrin-mediated signaling pathway" ppsx
Ngày tải lên :
10/08/2014, 10:20
... depletion of Rab5a influented the internalization and recycling of integrins from the trailing edge to the leading edge, and interrupted the ‘outside-in’ signaling The assembly and activities of the ... overexpression of Rab5a in cancer may enhance the integrin-mediated signaling pathway, and induce the cancer cell migration and invasion Page of Figure Rab5a regulates the activity of Rho GTPases and reorganization ... reconstruction of adhesion and actin cytoskeleton It is now widely accepted that the process of cancer cells migration mainly consists of four steps: the formation and extension of filopodium and lamellipodium...
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Báo cáo y học: "Antimelanogenic effect of c-phycocyanin through modulation of tyrosinase expression by upregulation of ERK and downregulation of p38 MAPK signaling pathway" docx
Ngày tải lên :
10/08/2014, 10:20
... antimelanogenic mechanism of Cpc The expression of tyrosinase and the production of melanin were determined to examine the antimelanogenic effect of Cpc The levels of signaling molecules such ... mechanisms: the promoted degradation of MITF protein through the up-regulation of MAPK/ERK signaling pathway, and the suppressed activation of CREB via the down-regulation of p38 MAPK pathway Cpc elevates ... upregulation of ERK and downregulation of p38 MAPK signaling pathways Journal of Biomedical Science 2011 18:74 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient...
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báo cáo khoa học: " Statin-induced apoptosis via the suppression of ERK1/2 and Akt activation by inhibition of the geranylgeranyl-pyrophosphate biosynthesis in glioblastoma" potx
Ngày tải lên :
10/08/2014, 10:21
... ratio of the number of living cells after 24, 48, and 72 h of incubation with 1, 2.5, 5, 10 μM mevastatin, 1, 2.5, 5, and 10 μM fluvastatin or 2.5, 5, 10, and 20 μM simvastatin to the number of ... the activation of ERK1/2 Akt [4] These findings are in agreement with the results of the present study and indicate that statins induce apoptosis via suppression of Ras/ERK and Ras /Akt pathways ... functions of Ras by inhibiting prenylation through the inhibition of GGPP synthesis; this enables localization of Ras at the plasma membrane [14,15] Ras is involved in the activation of the MEK/ERK and...
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Báo cáo y học: "Cancer control through principles of systems science, complexity, and chaos theory: A mode"
Ngày tải lên :
26/10/2012, 10:04
... emergence of order…[allowed by] a constant flow of energy and matter through the system…[the] emergence of new structures and new forms of behavior…[which are] the hallmark of self-organization, [and] ... one hand, shift the balance of system’s oscillations toward the outer edge of chaos and increase the potential for cancer growth, and on the other hand, it could choose the path of stability through ... analogous to the movement of sperms prior to fertilization The systems’ evolutionary phase follows the completion of the initial state but prior to a stage of a of the zone of order and the fully-functioning...
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The transference of meaning through class of words denoting parts of the human body in english and vietnames
Ngày tải lên :
18/12/2013, 21:45
... the type of transference which has taken place The eye of a man - The eye of a potato The bowel of a person - The bowel of the earth The hand of a child - The hand of a lock The heart of a man ... percentage of the forms of each types of semantic transference and the percentage of the types of semantic transference used in these classes Table 2: The percentage of the forms of each type of semantic ... war The arm of a person - The arm of jacket 10 One's breast - The chest 11 One's foot - The foot of sock 12 One's hands - The hands of a farm 13 The heel of a person - The heel of the hand 14 One's...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
Ngày tải lên :
18/02/2014, 14:20
... 85% inhibition of BHLmL2 and BHAb, respectively, while mm 2-mer- captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively The influence of pH on the activities of the BH enzymes ... concentrated and used for biochemical analysis SDS/PAGE of purified BHs Fig Effect of camalexin and 3-phenylindole on the activity of BH from mycelial cultures of Leptosphaeria maculans (A) and Alternaria ... multiple roles, including microbial growth inhibition and inhibition of certain fungal enzymes [7,10] The antifungal activity of brassinin (1) is partly a result of its dithiocarbamate group, known...
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