and or or nor does not occur at all

... Measurements of cells that were incubated at °C with antibody 2189 ⁄ 90, and then with an FITC-labeled secondary antibody at °C, and then at 37 °C for before trypsinization and analysis The data shown in ... the expression vectors was performed according to standard laboratory procedures and according to the protocols supplied with enzymes and kits Site-specific mutations were generated with a QuickChange ... located at the same position as after washing at pH 7.4, indicating that the different pH values of the buffers used in the incubations did not affect the size or the morphology of the cells, and...

Ngày tải lên: 23/03/2014, 07:20

... important since it is known that explanted mouse neural tissues latent for HSV-1 demonstrate viral reactivation in culture This suggests that explantation itself or factors in tissue culture that ... investigation opens up new vistas for studying herpesvirus latency and reactivation Methods Mice Female BALB/cJ and BALB/c scidJ mice at weeks of age (The Jackson Laboratory, Bar Harbor, ME) ... ImmunoResearch Laboratories, Inc., West Grove, PA) Following washing, the plate wells were incubated in tetramethylbenzidine substrate (DakoCytomation, Inc., Carpinteria, CA) for 15 minutes at...

Ngày tải lên: 19/06/2014, 08:20

... important since it is known that explanted mouse neural tissues latent for HSV-1 demonstrate viral reactivation in culture This suggests that explantation itself or factors in tissue culture that ... investigation opens up new vistas for studying herpesvirus latency and reactivation Methods Mice Female BALB/cJ and BALB/c scidJ mice at weeks of age (The Jackson Laboratory, Bar Harbor, ME) ... ImmunoResearch Laboratories, Inc., West Grove, PA) Following washing, the plate wells were incubated in tetramethylbenzidine substrate (DakoCytomation, Inc., Carpinteria, CA) for 15 minutes at...

Ngày tải lên: 20/06/2014, 04:20

... (H3/H4 acetylation, H3K4 methylation) and negatively correlated with inhibitory modifications (H3K27 trimethylation and DNA CpG methylation) [10] Subtle differences in the integration site choice ... pathogenesis and of the potential use of lentiviral vectors for gene therapy applications Results Generation and characterization of a cell line carrying a stably integrated lentiviral vector ... pHIV-Intro, pEYFP-MS2nls and either mDsRed tagged Tat (top) or Tat and mDsRed A) HeLa cells were cotransfected with pHIV-Intro, pEYFP-MS2nls and either mDsRed tagged Tat (top) or Tat and mDsRed tagged...

Ngày tải lên: 13/08/2014, 05:21

... from mature, monomeric VEGF), but not human placenta-derived growth factor, platelet-derived growth factor, or transforming growth factor Inter- and intra-assay variances for VEGF, sVEGFR-1, and ... mean age of 57 ± 12 years and a mean disease duration of ± 4.5 years (21 patients had a disease duration of less than years) The clinical and laboratory data for these patients are presented in ... nonparametric statistical methods: Mann–Whitney (unpaired data) and Wil- coxon (paired data) tests for comparison of groups, and Spearman's rank correlation test for assessment of the relationships...

Ngày tải lên: 09/08/2014, 01:23

... CG and GG genotypes in terms of mortality or development of ESRD in the total population under study (Table 3) Also, in separate analyses of normoalbuminuric patients and patients with nephropathy ... diabetic nephropathy patients and normoalbuminuric patients at baseline Dendooven et al Journal of Negative Results in BioMedicine 2011, 10:4 http://www.jnrbm.com/content/10/1/4 Normoalbuminuria ... -945 promoter polymorphism Diabetic Nephropathy Normoalbuminuria Allele C 472 (52.7) 453 (54.0) Allele G 424 (47.3) 385 (46.0) CTGF -945GC polymorphism Diabetic Nephropathy Normoalbuminuria CC...

Ngày tải lên: 11/08/2014, 07:21

... analysis and interpretation; and for drafting the manuscript HM was responsible for conception and design of the study; for acquisition of data, and its analysis and interpretation; and for drafting ... did not decrease to under 4.0 kPa before circulatory standstill took place [31] In a human validation study [32] a significant correlation between NIRS-measured StO2 and venous oxygen saturation ... oxygen saturation; SvO2, mixed venous oxygen saturation; VO2, oxygen consumption data were in concordance with a previous report from De Blasi and coworkers [16] Studies in animals and patients...

Ngày tải lên: 13/08/2014, 03:20

... distribution of professional status is however quite homogenous between both populations 10 Migratory status To build migratory status, we use information relating to the nationality and the country of birth ... field According to Putnam, social capital “refers to features of social organisation, such as trust, norms and networks that can improve the efficiency of society by facilitating coordinated actions” ... participation and health status according to occupation and age The estimation on the whole population (column 1) confirms that social participation is strongly and significantly associated with...

Ngày tải lên: 28/03/2014, 20:20

... IL) at room temperature for approximately minute and using Kodak BIOMAX MR films for exposure Normal human thyroid lysate was used as a positive control, and cells treated with GLV-1h68 and PBS ... noninvasively and serially identify sites of viral targeting and to measure the level of viral infection and spread in order to provide important information for correlation with safety, efficacy, and ... useful information regarding timing of viral dose and administration for optimization of therapy, as well as distribution and replication of the oncolytic virus, and would alleviate the need for multiple...

Ngày tải lên: 18/06/2014, 19:20

... IL) at room temperature for approximately minute and using Kodak BIOMAX MR films for exposure Normal human thyroid lysate was used as a positive control, and cells treated with GLV-1h68 and PBS ... noninvasively and serially identify sites of viral targeting and to measure the level of viral infection and spread in order to provide important information for correlation with safety, efficacy, and ... useful information regarding timing of viral dose and administration for optimization of therapy, as well as distribution and replication of the oncolytic virus, and would alleviate the need for multiple...

Ngày tải lên: 20/06/2014, 03:20

... weight, all three were sent for slaughter At the abattoir carcass weight, lean meat percentage, fat measurements and a health control of inner organs was recorded individually for every pig according ... group Treatment group Figure lesions (n = between daily weight gain and each of gastric Correlation indicates the number of pigs in score category) Correlation between daily weight gain and score ... reported that feed intake patterns influence fat and lean growth in pigs through an effect on utilisation of nutrients Both de Haer and de Vries and Ramaekers and co-workers [5,6] showed that...

Ngày tải lên: 12/08/2014, 18:22

... http://ccforum.com/content/6/5/452 Introduction Prone positioning for respiratory failure has recently gained popularity as an adjunct for the treatment of respiratory failure and adult respiratory ... coaxial rotation An intensivist, a respiratory therapist, and multiple nurses are present for all turning events Vital signs are monitored closely before and after the turn to ensure that the patient ... repositioned, and one was replaced for poor flow One femoral cannula was replaced for poor flow Discussion lae unrelated to prone position or turning In one patient, this occurred in the supine position and...

Ngày tải lên: 12/08/2014, 19:21

... responsible for the various multimeric forms observed Rather, it is more likely that one interface would lead to dimerization and another one to trimerization; together they would be responsible for hexamerization ... As2O3 does not modify the multimerization of TRIM5α and TRIMCyp Figure As2O3 does not modify the multimerization of TRIM5α and TRIMCyp A, human HeLa cells stably expressing TRIM5αrh and TRIMCyp, or ... (N-MLV; Fig 3B) No notable differences were found at the other glutaraldehyde concentrations Data from Fig and together suggest that the multimerization of TRIM5 proteins is not modulated by retroviral...

Ngày tải lên: 12/08/2014, 23:22

... calculations Statistical analysis Data are presented for the whole study period Data are presented as mean ± standard deviation for normally distributed data, or as median (interquartile range) for ... for sedation to maintain ventilator synchrony and comfort ('education') The same strategy was employed for the ICU nurse team This intervention was repeated three times Finally, the patient data ... Netherlands) was equipped with a special tool that automatically calculated the ideal VT from patient's height, after which the targets were automatically generated in the 'respiratory tab' (for all...

Ngày tải lên: 13/08/2014, 08:20

... -AACTTCCGCTCCAGCAAT-, antisense: -TCCCGAGGCTTCATTCAT- Conditions for realtime PCR were as follows: 10 denaturing at 95°C, 10 s annealing at 64 or 62°C, and 5–9 s amplification at 72°C Forty cycles were performed ... early and a late state of activation We used 1st passage and 4th passage HSC as an early and Page of 12 (page number not for citation purposes) Comparative Hepatology 2006, 5:5 http://www.comparative-hepatology.com/content/5/1/5 ... apoptosis and proliferation of activated rat hepatic stellate cells Hepatology 1999, 30:196-202 Gandhi CR, Kuddus RH, Uemura T, Rao AS: Endothelin stimulates transforming growth factor-beta1 and collagen...

Ngày tải lên: 13/08/2014, 13:20

... those generally accepted and applied by auditors in other countries Accordingly, the French financial statements and the auditor's and statutory auditor's attestation of which a translation is ... unconditional, unsubordinated and unsecured obligations of the Company, and rank equally amongst themselves and pari passu with all others, present or future unsecured and unsubordinated obligations of ... incorporated by reference into this final prospectus This information remains, at the date of this final prospectus, accurate - 40 - Translation into English for information purposes only - Original...

Ngày tải lên: 15/03/2014, 07:20

... services and to take, in the light of their results, an informed decision as to whether or not to seek an abortion Given that Polish domestic law allowed for abortion in cases of foetal malformation, ... particular that the applicants had been given misleading and contradictory information and had not received objective medical counselling; and, the fact that access to abortion was a subject of heated ... obtaining a legal abortion in Ireland, they had to go to the United Kingdom for an abortion and that the procedure was humiliating, stigmatising and risked damaging their health Having or helping anyone...

Ngày tải lên: 28/03/2014, 16:20

... record Note: Be careful with the NOT operator You would exclude works that are both on goats and sheep, thus miss useful information on goats Database management systems - Textual databases and ... query goats + sheep retrieves records where either goats or sheep occur, or both Addition NOT ^ Exclusion a query goats ^ sheep retrieves all records where goats occurs, unless sheep occurs in ... “logical” or “ boolean” operators The three most important ones are: Operator Action AND ISIS Syntax * Example a query goats * sheep retrieves records where both goats and sheep occurs Intersection OR...

Ngày tải lên: 31/03/2014, 20:20

... platform and its controller enable investigators to precisely control the platform displacement and acceleration The use of a non-contact linear motor and air bearing slides essentially eliminates ... horizontal translating force platform and data collection system [14] The SLIPFALLS platform was specifically designed and built to assess psychophysical thresholds to postural perturbations This ... adaptive alternative forced choice (2AFC) protocol [16], the acceleration thresholds for detecting an anterior-posterior, horizontal translation of the platform at displacements of mm, mm, and 16 mm...

Ngày tải lên: 19/06/2014, 08:20

... abrogate any acute or subtle signaling events mediated through the IL-1 R1 receptor Certainly, it is possible that other receptors for IL-1 may compensate for the lack for IL-1 R1 in this situation ... activation, on IL-1-mediated inflammatory responses following Aβ vaccination, and on Aβ deposition during normal aging, we used interleukin-1 receptor 1-knockout (IL-1 R1-/-) mice [40-42] that ... interleukin-1 receptor, but develop normally Moreover, with a few exceptions, these mice are normal, showing alterations in IL-1-mediated immune response to certain stimuli Following penetrating brain...

Ngày tải lên: 19/06/2014, 22:20