and endokinins induced responses in human airways

... responses in guinea pig airways Contractile effects of hHK-1 and endokinins in isolated guinea pig airways Human hemokinin-1 induced concentration-dependent contractions of the guinea-pig trachea ... sample of human fetal brain total mRNA (Ozyme, Saint Quentin en Yvelines, France) was also included in each run In vitro bronchomotor responses Human bronchial rings and guinea pig tracheal and bronchial ... bronchi Our findings indicate that hHK-1 could induce contraction of human bronchi and guinea pig airways This hHK-1 -induced contraction could be mainly attributed to NK2 receptors in humans and to...

Ngày tải lên: 12/08/2014, 11:22

... signal-regulated kinases (ERK1/2), stress-activated protein kinases [c-Jun NH2-terminal kinases (JNK)], and p38 mitogen-activated protein kinases (p38MAPK) ERK1/2 are activated mainly by growth factors and ... aspects in the regulation of apoptosis is the involvement of mitogen-activated protein kinases (MAPKs), a family of serine/threonine kinases that mediate intracellular signal transduction in response ... DNA ladder induced by Py/Zn treatment (Fig 1D) To determine the relationship between intracellular Zn levels and induction of apoptosis, we investigate the appearance of a DNA ladder induced by...

Ngày tải lên: 23/03/2014, 21:20

... attenuates the inflammatory stimuli -induced activation and DNA binding activity of NF-κB in human chondrocytes In order to gain further insight into the mechanism, we used an in vitro kinase activity ... ligands including S100 protein, high-mobility group box chromosomal protein and AGEs (complex and specific AGEs) in OA chondrocytes and synoviocytes results in increased production of various inflammatory ... VM, Haqqi TM: Epigallocatechin-3-gallate selectively inhibits interleukin-1 -induced activation of mitogen activated protein kinase subgroup c-Jun N-terminal kinase in human osteoarthritis chondrocytes...

Ngày tải lên: 09/08/2014, 14:20

... grown and maintained in Eagles Minimal Essential Media (MEM) containing 2% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin (all from Gibco, Life Technology) Infection of cell culture with influenza ... phase of H1N1 infection TNF-a was induced by all subtypes beginning at the early phase of infection A 3-fold increase in TNF-a secretion in late H5N1/2004 infection was also observed, and these results ... qualitative and quantitative differences in the profiles of cytokines/chemokines induced by influenza viruses of different pathogenicity H5N1 was a more potent inducer of inflammatory cytokines/chemokines;...

Ngày tải lên: 12/08/2014, 02:20

... beginning and end of each branch of G4, and at the beginning of the branches in G5 The labeling of the planes and profiles was in accordance to the generation in which the plane or profile was in ... flow at the inlet was assumed to be inspiratory, laminar and incompressible Using FLUENT 6.1, the inlet Reynolds numbers of 514, 1070 and 2194 were chosen to represent resting, light and moderate ... xii Summary 1 Introduction and Scope 2 Literature Survey 2.1 Flow in Curved Tubes 2.2 Flow in Single Bifurcated Airways 2.3 Factors Affecting Particle Deposition in Single Bifurcated Airways 2.4...

Ngày tải lên: 26/09/2015, 11:07

... paintings had been a well-known phenomenon since Roman times, and such paintings would create the illusion of, for instance, walls containing a window to the outside world or a ceiling containing ... rendering Image cube trajectory Institute of Electrical and Electronics Engineers Internet Engineering Task Force Interaural intensity difference Interaural level difference Infrared light-emitting ... multidisciplinary perspective that integrates media design and engineering, multisensory perception, and social psychology Add to this the increasingly social nature of interfaces and the increase in...

Ngày tải lên: 12/05/2013, 10:21

... cationic amino acids lysine, arginine and ornithine at the basolateral membrane of the polar epithelial cells in the intestine and renal tubules LPI is caused by mutations in the SLC7A7 gene encoding ... in which the L-leucine effect was tested (B), L-leucine and L-arginine were added simultaneously After min, incubation was stopped and L-arginine uptake measured as described in Experimental procedures ... experiments in the absence of Na+ were performed L-Leucine was ineffective on + L-arginine in ux in the absence of Na , confirming the presence of the system y+L in human platelets (Fig 2) Data indicate...

Ngày tải lên: 08/03/2014, 02:20

... proteins into the ER [18] The strong inhibition of the processing of pro-cathepsin E into the mature enzyme by brefeldin A indicates that the processing and maturation of this protein occur in ... propeptides, and N-glycosylation of cathepsin E in its processing, maturation, and trafficking must be of particular importance In this study, to understand the molecular basis of the processing and intracellular ... oligosaccharides Cathepsin E is also detected in the ER and Golgi complex in various cell types, including gastric cells [12,13], human M cells [5], Langerhans cells, and interdigitating reticulum cells...

Ngày tải lên: 16/03/2014, 14:20

... In Eurospeech '97 M Swerts and M Ostendorf 1995 Discourse prosody in human- machine interactions In Proceedings of the ECSA Tutorial and Research Workshop on Spoken Dialog Systems - Theories and ... found, and this difference can easily be explained by the combination of two simple trends First, there was a decrease in the number of final rising contours, and second, t h e r e were increases in ... accent and contour had shown some utility in identifying certain discourse relations [ (Pierrehumbert and Hirschberg, 1990), (Hirschberg and Litman, 1993) Although changes in pitch maxima and minima...

Ngày tải lên: 17/03/2014, 07:20

... data points in the F2 dimension, 16–32 transients and 720–1024 complex increments in the F1 dimension Raw data were multiplied in both dimensions by a pure cosine-squared bell window function and ... studies carried out in 40% TFE in an H2O mixture [38] According to studies of CRH binding to single bilayer egg phosphatidylcholine vesicles, the development of the intramolecular interaction with ... E.B., Insel, T.R., Perrin, M.H., Rivier, J., Vale, W.W & Kuhar, M.J (1985) Differential regulation of corticotropinreleasing factor receptors in anterior and intermediate lobes of pituitary and in...

Ngày tải lên: 17/03/2014, 10:20

... Ca2+ in ux Effect of EPCG, catechin hydrate, curcumin, apigenin, naringenin and gallotannin on QUIN -induced intracellular Ca2+ in cultured human neurons As mentioned above, QUIN stimulation induced ... increased Ca2+ in ux was greatest with EPCG and curcumin 372 compared with apigenin and naringenin (Fig 5) Interestingly, catechin hydrate did not ameliorate a QUIN -induced increase in intracellular ... catechin hydrate, curcumin, apigenin, naringenin and gallotannin on QUIN -induced nNOS activity and extracellular nitrite production in human neurons Effect of EPCG, catechin hydrate, curcumin,...

Ngày tải lên: 22/03/2014, 21:20

... indomethacin, methotrexate and E217bG was 77.3 ± 5.2 pmolÆmg)1 The control uptake for the remaining drugs was 136.5 ± pmolÆ mg)1 Compound GSH Vincristine + mM GSH Calcein + mM GSH Indomethacin ... Methotrexate and E217bG were found to inhibit the cGMP uptake in human erythrocyte vesicles with E217bG at 65 lM inhibiting about 90% and methotrexate inhibiting about 75% at 275 lM and completely ... The L23/R and MOR/R sublines were maintained in the presence of 0.2 lgÆmL)1 and 0.4 lgÆmL)1 doxorubicin, respectively The cells were kept in drug-free medium for at least 48 h before use in experiments...

Ngày tải lên: 23/03/2014, 21:20

... including malarial infection and Parkinson’s disease [42] Harmine and related b-carbolines have cytotoxic activity towards human tumour cell lines in culture [43], suggesting a possible use in ... suggesting a role in neurogenesis like its Drosophila orthologue, minibrain (mnb) [4] Studies in both mouse and Drosophila have found an important role for DYRK1A ⁄ minibrain kinase in determining ... cell lysate was retained as an input sample and FLAG-immunoprecipitations were performed on the remainder *Indicates bands resulting from IgG; arrows indicate bands of interest In (B–D), cell lysates...

Ngày tải lên: 30/03/2014, 02:20

... significant And, as system developers move toward more closely modeling human- human interaction, the percentage is likely to rise The basic aspects of the notation include marking the interruption point, ... varying lengths, it pulled out 500 as possibly containing a repair and missed 97 sentences actually containing a repair Of the 500 that it proposed as containing a repair, 62% actually did and ... can be helpful in detecting repairs is acoustics In this section we describe firsthow prosodic information can help in distinguishing repairs from false positives for patterns involving matched...

Ngày tải lên: 31/03/2014, 06:20

... chemotactic interaction during hematopoietic stem cell mobilization induced by G-CSF or cyclophosphamide J Clin Inves 2003, 111:187-196 69 Cavaillon JMH-CN: Signals involved in interleukin synthesis and ... subsequent studies have expanded this finding to situations of inflammation [44], and peripheral tissue injury [45-47] Hematopoietic stem cells are being increasingly recognized as having diverse non-hematopoietic ... functions including production of angiogenic cytokines [48], and acting as an “innate” immune cell capable of rapidly differentiating into dendritic cells for protection of the host against infections...

Ngày tải lên: 18/06/2014, 16:20

... obtained from heparinised rabbit blood and used for CTLs and cytokine assays; the PBMCs were grown in RPMI supplemented with glutamine, 10% FCS, and P/S Rabbit skin fibroblasts were obtained ... priming and after the E7 protein boosting IFN-γ and IL-4 levels are expressed as fold-differences vs baseline, obtained from non-stimulated pre-immune PBMCs, and normalized against β-actin expression ... JT: HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes EMBO J 1989, 8:3905-3910 Duensing S, Münger K: Mechanisms of genomic instability in human cancer: insights from...

Ngày tải lên: 18/06/2014, 16:20

... cells in tissue culture provides a potential mechanism for increasing invasive mammary carcinoma [5] and induced colonic neoplasia in animal models [6] Borax is used as a food additive in some ... (Invitrogen, Carlsbad, CA, USA) and penicillin-streptomycin antibiotic Phytohemagglutinin was added in RPMI medium as the mitogen and incubated for 72 hours After 72 hours incubation, a total of × 105 ... nephrotoxicity and neurotoxicity in human but the borax toxicity about human genetic materials and humoral immune cell still need to be investigated for food additive carcinogenesis The defect in genetic...

Ngày tải lên: 20/06/2014, 00:20

... Low) in HCV polyprotein expressing cells and in staurosporine treated cells, in contrast with cells infected with the VT7-HCV7.9 in the absence of IPTG or in uninfected cells These results indicate ... shown in Fig 5A, the cytochrome c remained confined to the mitochondria in both uninfected cells and VT7-HCV7.9infected cells in the absence of IPTG However, in cells infected with VT7HCV7.9 in ... labelling on ultrathin sections Due to the fixation and embedding procedures used in immunostaining, the cell structures are less visible than by embedding in an epoxy resin While in cells infected with...

Ngày tải lên: 20/06/2014, 01:20

... significant, increase in levels of proMMP-2 and active MMP-2 in the media C2-ceramide -induced regulation of MMP-2 is abrogated by inhibition of PKR R48 To determine a role for PKR in ceramide -induced ... of protein synthesis and increased apoptosis, which may also affect cartilage integrity Increased expression and activation of MMPs, in the absence of a corresponding increase in their inhibitors, ... protein kinase R (PKR)activating protein (PACT) [14] is up-regulated in regions of cartilage that subsequently develop osteoarthritis-like changes in vivo [15] and that PACT and PKR are involved in...

Ngày tải lên: 09/08/2014, 01:23

... 1996, 39:968-978 Morales TI: The insulin-like growth factor binding proteins in uncultured human cartilage Increases in insulin-like growth factor binding protein during osteoarthritis Arthritis ... (IGF)-1 to IGF-binding proteins, on 125I-labeledinsulinbinding NBI-31772, a small-molecule inhibitor like growth factor (IGF)-1 to IGF-binding proteins, on 125I-labeled IGF-1 binding to human osteoarthritic ... washes of minutes each in 10 mM Tris-buffered saline, pH 7.4, containing 0.15 M NaCl (TBS), membranes were incubated for 30 minutes in TBS containing 3% NP40 and blocked in TBS containing 0.5%...

Ngày tải lên: 09/08/2014, 01:23