... tem- plate to introduce, by PCR, the mutation K96R. After amplification the fragment was purified and subcloned into pET3d vector and DNA sequenced for confirma- tion. The resulting plasmid was used to ... containing a second CX 3 C motif. From top to bottom (with database accession numbers in parentheses): Sulfolobus solfataricus (P95896); Pseudomonas syringae pv. tomato str. DC3000 (AAO55930); Pseudomonas ... solfataricus belongs to the CX 3 C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles Elisa Cilia 1,2 , Armando Fabbri 1 , Monica...
Ngày tải lên: 07/03/2014, 21:20
... had difficulty accessing needed dental care reportedly were unable to get care because the provider refused to accept their insurance plan, compared to only 2 percent of privately insured children. ... charges, access to care, satisfaction with care, health insurance coverage, income, and employment. We analyzed responses to questions on the use of dental care as well as responses to questions ... necessary to pool together more than 1 year of MEPS data to yield sample sizes large enough to generate reliable estimates. To facilitate use of these data and the calculation of designed-based standard...
Ngày tải lên: 14/03/2014, 09:20
Báo cáo Y học: The unorthodox histidine kinases BvgS and EvgS are responsive to the oxidation status of a quinone electron carrier ppt
... of ubiquinone-0 (Q-0) (1 l M to 5 m M ), MK-3 (1 l M to 1 m M ), oxidized and reduced FAD and NAD (1 l M to 5 m M ), nicotinic acid (1 m M to 10 m M )orMgSO 4 (1 l M to 50 m M ). In the case of menadione, ... Georgellis and coworkers [22], the quinones are membrane localized electron transporters which are interesting candidates as redox indicators for sensor proteins such as ArcB, BvgS and EvgS. As ... BvgS and EvgS are responsive to the oxidation status of a quinone electron carrier Andreas Bock and Roy Gross Lehrstuhl fu ¨ r Mikrobiologie, Biozentrum, Universita ¨ tWu ¨ rzburg, Am Hubland,...
Ngày tải lên: 17/03/2014, 23:20
Describe wireless wide area networks (WWANs) and how they are used
... available bandwidth between the users • Business class service – Offers dedicated channels with dedicated bandwidth – Types of connectivity • Point -to- point, point -to- multipoint, and multipoint -to- multipoint 5 How ... Broadband Wireless • Use of satellites for personal wireless communication is fairly recent • Satellite use falls into three broad categories – Satellites are used to acquire scientific data and ... widely used applications – Allows for the delivery of short, text-based messages between wireless devices • Messages are limited to about 160 characters – Applications • Person -to- person • Agent -to- person • Information...
Ngày tải lên: 13/09/2012, 10:52
Tài liệu In this lab, 2 ISDN routers are required. If ISDN routers are not available, review the lab to become familiar with the process. An Adtran Atlas550 ISDN emulator is used to simulate the switch/ISDN cloud. pdf
... number to use to contact this remote device must also be specified. Use the following commands to do this: Tokyo(config)#interface dialer 1 Tokyo(config-if)#dialer remote-name Moscow Tokyo(config-if)#dialer ... Define switch type and spid numbers To configure the switch type and spid numbers use the following commands. Router(config)#hostname Tokyo Tokyo(config)#enable secret class Tokyo(config)#isdn ... Tokyo(config-if)#interface dialer 1 Tokyo(config-if)#encapsulation ppp Tokyo(config-if)#ppp authentication chap Tokyo(config-if)#interface dialer 2 Tokyo(config-if)#encapsulation ppp Tokyo(config-if)#ppp...
Ngày tải lên: 21/12/2013, 19:15
Tài liệu Are Corruption and Taxation Really Harmful to Growth? docx
... growth and corruption are likely to be jointly determined. Below we discuss how we attempt to deal with these issues. If bureaucrats can customize the nature and amount of harassment on firms to extract ... construction and tourism). The five sectors could be further classified into 14 three-digit ISIC-categories. Based on number of enterprises, the five sectors constituted 52 % of the private sector, and ... taxes and grow faster. 11 instrument contained roughly 150 questions and a handful were related to corruption. Fifth, each firm was typically visited at least twice by one or two enumerators (to accommodate...
Ngày tải lên: 23/12/2013, 06:17
Tài liệu Edit Data and Update Changes That Are Made to an ADO.NET pdf
... 4.1 Edit Data and Update Changes That Are Made to an ADO.NET DataSet Object Listing and viewing data is easy. What you really need to do is to be able to edit and update data. You know ... then stored back into the server, and the text boxes are changed to disabled. If the btnCancel is clicked, the text boxes are changed to disabled. Comments Although it takes a bit more code to ... Open and run the VB.NET-Chapter 4 solution. From the main form, click on the command button with the caption How -To 4.1. When the form loads, click on the Load List button to display the customers...
Ngày tải lên: 24/12/2013, 06:17
Tài liệu Are Corruption and Taxation Really Harmful to Growth? Firm Level Evidence doc
... growth and corruption are likely to be jointly determined. Below we discuss how we attempt to deal with these issues. If bureaucrats can customize the nature and amount of harassment on firms to extract ... construction and tourism). The five sectors could be further classified into 14 three-digit ISIC-categories. Based on number of enterprises, the five sectors constituted 52 % of the private sector, and ... Profit, and Risk”, in R. Reinikka and P. Collier (eds.), 2001, Uganda’s Recovery: The Role of Farms, Firms, and Government, Washington DC: The World Bank. Ruzindana, A., Langseth, P., Gakwandi,...
Ngày tải lên: 16/01/2014, 16:34
Tài liệu Báo cáo khoa học: PC1⁄3, PC2 and PC5⁄6A are targeted to dense core secretory granules by a common mechanism doc
... factors, and viruses. PC1 ⁄ 3, PC2 and PC5 ⁄ 6A are the only members of the PC family that are targeted to dense core secretory granules, where they carry out the process- ing of proteins that are ... secretory granules by PC enzymes such as PC1 ⁄ 3, PC2 or PC5 ⁄ 6A. Understand- ing how these enzymes and their substrates are tar- geted to secretory granules is thus critical in understanding ... 008792) and mouse PC5 ⁄ 6A (BC12619). The numbering used to identify the protein domains used is relative to the initiator methionine. Protein fragments were tested for their ability to sort heterologous...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: "Which words are hard to recognize? Prosodic, lexical, and disfluency factors that increase ASR error rates" ppt
... so- lution is to assign any insertion errors to each of 1 These conversations are not part of the standard Fisher and Switchboard corpora used to train most ASR systems. Ins Del Sub Total % data Full ... Edinburgh-Stanford LINK and ONR MURI award N000140510388. We thank Andreas Stolcke for providing the ASR out- put, language model, and forced alignments used here, and Raghunandan Kumaran and Katrin Kirch- hoff ... distributed random vari- able, with mean equal to the population mean, and variance to be estimated by the model. Stated dif- ferently, a random effect allows us to add a factor to the model...
Ngày tải lên: 20/02/2014, 09:20
Food and Beverage Marketing to Children and Adolescents: What Changes are Needed to Promote Healthy Eating Habits? ppt
... regulations. 42 Food and Beverage Marketing to Children and Adolescents: What Changes are Needed to Promote Healthy Eating Habits? Although many social, cultural and environmental factors inuence children’s and ... snacks, and ■ some restaurants have pledged to abandon supersized portions. Proctor and Gamble, General Mills and Nabisco are among those selling 100-calorie packs of cookies, crackers and popcorn. Logos ... television and restrict tobacco advertising in magazines? How can previous work on tobacco advertising be ■ used to inform future efforts to reduce food and beverage marketing? Prepared by Nicole...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: Intrinsic local disorder and a network of charge–charge interactions are key to actinoporin membrane disruption and cytotoxicity ppt
... wild-type protein (B) and the StnII-R29Q (C) and StnII-Y111N (D) variants. Blue and red correspond to positively and negatively charged areas, respectively. The side chains of R29 and Y111 are shown in ... incorporated into the structure calculation protocol. Initially, 100 conformers were generated that were forced to satisfy the experimental data during a standard automatic cyana protocol based ... cytolytic toxins from sea anemones (Actiniaria). FEMS Microbiol Immunol 5, 121–129. 2 Anderluh G & Macek P (2002) Cytolytic peptide and protein toxins from sea anemones (Anthozoa: Actinia- ria). Toxicon...
Ngày tải lên: 14/03/2014, 23:20
50% OFF ALL CHARLTONS OAK FURNITURE THIS MONTH ONLY! ALL PRICES INCLUDE VAT AND ARE SUBJECT TO STOCK AVAILABILITY. docx
... n/a CMIRR1200 Handle Options (wood as standard) Victorian chrome handle (each) £8.00 n/a £4.00 n/a KNOBCH Pewter finish handle (each) £4.00 n/a £2.00 n/a KNOBPEW Brass finish handle (each) £4.00 ... 01865 367888 www.everythingwood.org.uk 50% OFF ALL CHARLTONS OAK FURNITURE THIS MONTH ONLY! ALL PRICES INCLUDE VAT AND ARE SUBJECT TO STOCK AVAILABILITY. Bretagne Collection Tables Code Extending ... £288.00 £144.00 £158.00 £168.00 Farrington chair with fabric padded seat £220.00 £110.00 n/a n/a FOCH Farrington chair with leather seat (selection 1) £260.00 £130.00 n/a n/a Farrington chair with Saddle leather...
Ngày tải lên: 15/03/2014, 23:20
Báo cáo khoa học: Cytosolic phospholipase A2-a and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus pdf
... binds selectively to a-d-mannosyl and a -d-glucosyl residues, was used to label the ER and nuclear envelope (Fig. 2). The results A B D C Fig. 1. cPLA 2 -a expression levels and sub- cellular ... found to be coupled to both COX-1 and COX-2 to produce prostaglandin E 2 [51]. The physical colocalization of COX and cPLA 2 -a in these systems, however, has not been studied, and this is one of ... cPLA 2 -a and convert arachidonic acid into prostaglandin H 2 [8,9]. To date, two distinct COX iso- forms, COX-1 and COX-2, have been identified and characterized, and an alternative splice variant of COX-1,...
Ngày tải lên: 16/03/2014, 18:20
The a and b adapters are used as priming sites for both amplification
Ngày tải lên: 19/03/2014, 22:32
Authoring and Generating Health-Education Documents That Are Tailored to the Needs of the Individual Patient doc
Ngày tải lên: 22/03/2014, 15:21
New resource additions to the IDEAS Maternal and Child Health library during June to September 2012 ppt
Ngày tải lên: 23/03/2014, 06:20
The Future Isn’t What It Used To Be Changing Trends And Their Implications For Transport Planning doc
Ngày tải lên: 23/03/2014, 10:20
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