and biological activity of isothiocyanates and indoles
Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5 Structure of cyclic hexapeptides – cYT peptide The NMR data of the cYT peptide were indicative of its flexible nature ... biological activity and to study the effect of reducing the chain length of peptides on Ó FEBS 2004 Design of peptides for T-cell adhesion inhibition (Eur J Biochem 271) 2879 biological activity, ... intensity of ROE cross-peaks, and were assigned as strong, medium and weak ˚ represent the family Based on ROE violation > 0.2 A and allowed values of /, w in the Ramachandran map, only one family of...
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Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt
... presence of minute amounts of endotoxins The comparison of LPS and MfGl-II in the LAL assay shows, as expected, activity of LPS in the range of down to 10 pgÆmL)1, whereas MfGl-II has only activity ... respectively [23,30], and the bands at Fig Infrared spectrum in the spectral range 1800–900 cm)1 (A) and – in the range of the negatively charged phosphate band mas (PO2 ) 1300– )1 1190 cm (B) of hydrated ... water-binding capacity of lecithin headgroups [30] Finally, MfGl-II exhibits a main band at 1245 cm)1 and further weak bands at 1220 and 1260 cm)1 Thus, the phosphate groups of this glycolipid are...
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Tài liệu Báo cáo khoa học: Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas From target to transcript ppt
... (or both) of the above-mentioned processes may be relevant to moult control, we first investigated the biological activity of MIH and CHH during precisely timed stages of the moult cycle of Carcinus ... MIH content of the SG, relative to CHH, during late premoult Expression patterns of MIH and CHH in the X-organ during the moult cycle Expression patterns of mRNA levels of both MIH and CHH were ... investigated levels of MIH and CHH peptides in the sinus gland and levels of transcripts in XO neurones during the moult cycle, to see whether significant events, such as up- or down-regulation of transcription...
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Báo cáo khoa học: Construction and biological activity of a full-length molecular clone of human Torque teno virus (TTV) genotype 6 pptx
... [19], bone marrow [10] and several other organs [20] However, the main host cells and the target organs of this virus are still undefined The study of the biological functions of TTV is particularly ... interactions, and to find permissive cell lines for further molecular and cell biological studies of this peculiar human virus For this purpose, we have cloned and sequenced the full-length genome of TTV ... SalI double-digestions of the input linear construct yielded three restriction fragments of approximately 2100, 740 and 890 bp with the first enzyme pair, and of 2200, 630 and 890 bp with the latter...
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Báo cáo khoa học: Enhancement of intracellular concentration and biological activity of PNA after conjugation with a cell-penetrating synthetic model peptide docx
... influence of nociception on the beating rate of CM measured on day of culture without and with pretreatment with either 0.2 lM of I or II on day and after seeding The basal beating rate of the CM ... down-regulation of the nociceptin/orphanin FQ receptor to be the mechanism of biological activity of compounds I and II The antisense pretreatment remained without any influence on the basal beating rate of ... involvement of nonendocytic mechanisms (Fig 3) On the other hand, energy-dependent and -independent mechanisms contributed differently to the cellular uptake of the naked PNA and of the conjugate...
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Báo cáo Y học: Synthesis, conformational analysis and biological activity of cyclic analogs of the octadecaneuropeptide ODN Design of a potent endozepine antagonist pot
... analysis of the calculated structures revealed a short distances between NH of Leu7 and the side chain carboxyl group of the Asp6 residue (< 0.28 nm), and NH of Leu7 residue and C¼O of Leu5 (< ... of a potent endozepine antagonist (Eur J Biochem 268) 6049 Fig RP-HPLC monitoring of N- and C-terminal deprotections and on-resin cyclization of the linear precursor of cyclo128 OP Aliquots of ... the receptor affinity and selectivity of peptide ligands [13,14,27] The Ala-scan of OP has revealed that the side chain of each residue is required for the full activity of the peptide [12] These...
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báo cáo khoa học: " Biological activity of a genetically modified BMP-2 variant with inhibitory activity" potx
... in vitro evaluation of their biological activity, using ALP activity as a marker, revealed alterated effects for mutants of epitope and epitope as well But only alterations of epitope lead to ... established, using doses of 10, 40 and 160 μg Thus the number of animals was n = 18 After a period of 28 days the animals were sacrificed and the specimens were explanted Examination of the implants After ... method described by Kuberasampath and Ridge [12] The cylindric carriers with a diameter of mm and a length of 10 mm were autoclaved, soaked with the protein solution and lyophilized Animal studies...
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Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt
... the ADP ribosylation activity of ExoS and even more intriguingly, appears to be independent of serinephosphorylation [15,26] The amino acid sequence between 419 and 428 of ExoS is known to be ... 1, 2.5 lg of GST-ExoS(wt); lane 2, 1.25 lg of GST-ExoS(wt); lane 3, 0.75 lg of GST-ExoS(wt); lane 4, 2.5 lg of GST-ExoS(L426A); lane 5, 1.25 lg of GST-ExoS(L426A); lane 6, 0.75 lg of GST-ExoS(L426A); ... 2.5 lg of GST-ExoS(D427A); lane 8, 1.25 lg of GST-ExoS(D427A); lane 9, 0.75 lg of GST-ExoS(D427A); lane 10, 2.5 lg of GST-ExoS(L428A); lane 11, 1.25 lg of GST-ExoS(L428A); lane 12, 0.75 lg of GST-ExoS(L428A);...
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Báo cáo khoa học: Casein phosphopeptide promotion of calcium uptake in HT-29 cells ) relationship between biological activity and supramolecular structure ppt
... markers [alkaline phosphatase (ALP) and sucrase-isomaltase] and the rate of proliferation, and by electron-microscopic examination As shown in Fig 1A, the levels of ALP and sucrase-isomaltase in RPMI ... affect biological and laser light scattering measurements, we first explored whether the presence of phosphate was necessary for the biological effect of CPP To this end, a first dose–response set of ... individual CPPs, b-CN(1–25)4P and as1-CN(59–79)5P, (A) and (C), respectively, and of a mixture of CPPs (CPP DMV) (B) Each point on the graphs corresponds to the mean value of the [Ca2+]i peak rise...
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Báo cáo vật lý: "Resveratrol Derivatives from Stem Bark of Hopea and Their Biological Activity Test" docx
... isolated from the stem bark of Hopea consist of dimer, trimer and tetramer resveratrol Some compounds have biological activity as antioxidant and cytotoxic effect against Raji and HeLa-S3 cell lines ... For oligoresveratrol, that activity as hydroxyl radical scavenger was due to the existence of phenol ring, stability of molecular structure and existence of double bonds of olefinic unit Phenol ... (rings of A1 and C1), and one unit of a 1,2,4-trisubstituted benzene at δ 6.07 (1H, d, J = 2.6 Hz); 6.02 (1H, d, J = 8.4 Hz) Additionally, the 1H NMR spectrum exhibited signals for a set of aromatic...
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Development of a crosslinkable biomimetic collagen for mimicry of molecular architecture, biological activity and applications
... RGD and GFOGER (Knight et al., 1998; Knight et al., 2000), may result in the loss of full biological activity because of the absence of supplementary functional domains, such as synergy sites and ... diversity of collagen families makes development of a comprehensive, single model system challenging A system capable of emulating specific variables, such as the structure and biological activity, of ... chromatograms of purified (a) PT-CMP1, (b) PT-CMP2, (c) PT-CMP3, and (d) PT-CMP4 and their respective MALDI-TOF MS spectra (left) HPLC buffer gradient is from 90 %A and 10 %B to 55 %A and 45 %B in...
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ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
... microprofiles of O2, NH4+, NO2-, and NO3- in the autotrophic nitrifying biofilm (B) The distribution and magnitude of the estimated specific rates of net consumption and production of NH4+, NO2-, and ... organization of nitrifying bacterial populations and the in situ activity of these populations within the biofilms was discussed Microsensors For analysis of microbial structure and function (activity) of ... only net chemical profiles, and the spatial resolution is also above a single-cell level To address the question of the higher abundance and activity of SRB in oxic zones of biofilms (Okabe et al.,...
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Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt
... between EGF-induced reduction in Bid and tBid levels and the ubiquitin ligase activity of Itch In this study, we first examined the ability of Itch to interact with Bid and tBid We found that Itch specifically ... inset) n = conditions [29] Treatment of HEK-293T cells with TRAIL is known to induce caspase activity and cleavage of Bid in tBid [30] In our hands, treatment of HEK-293T cells with 200 ngÆmL)1 ... ± 0,02% of control; P = 0.001 for cell survival and 0.94 ± 0.03 of control for caspase activity) , and treatment with EGF slightly increased this effect on cell survival (88.4 ± 2.0% of control;...
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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf
... Potential role of metal-binding/exchange properties of GIF in AD One of the primary pathological hallmarks of AD is the formation of b-amyloid (Ab) plaques, composed primarily of Ab(1–40) and Ab(1–42) ... levels of extracellular GIF would allow ROS-mediated attack on astrocytes and neurons, and may also promote excessive neurite sprouting Metal-binding properties of GIF and role in synaptic activity ... survival of cultured cortical neurons [31] The neuroinhibitory action of GIF is very specific to this MT isoform and can be attributed to the small differences in the protein sequence of this isoform...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
... selection of recombinants The sites incorporated were HaeIII, NcoI and BamHI (Table 1) The PCR mix contained 200 ng of each primer, 20 ng of the template, 200 lm of each dNTP and units of Taq DNA ... subunit dissociation [27] and also in maintaining the geometry of the active site The availability of crystal structures of TIMs from 21 sources and the large database of TIM sequences from various ... 5.5 and w = )144.1 7.0 [53] The distribution of the / and w values of all other Lys residues in the TIM structure is shown for comparison The possible role of energetically unfavorable Ramachandran...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... understanding of the generic principle of catalysis for this family of proteases Results and Discussion Fold of ervatamin-A The overall structure and fold of ervatamin-A is similar to that of ervatamin-B, ... [27,35] and other members of the papain family, and is made up of two domains, L and R, with a V-shaped active site cleft at the interface of the domains (Fig 1) Substrate specificity and activity of ... investigation of the catalytic mechanism and substrate specificity and to understand the differences therein for this class of enzymes In order to identify the subsites of the ervatamins and to understand...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf
... Electron micrographs of a-synucleinA53T at the end of the assay confirmed the formation of fibrils, which were long (between and nm), straight and unbranched (Fig 6C,D) Addition of Gly and Lys-HCl at ... the effect of various additives and ⁄ or modifications on the overall activity of chaperone proteins Of the target proteins tested, Arg-HCl was found to specifically increase the activity of aB-crystallin ... no influence of the additive on the chaperone activity of aB-crystallin (i.e the effect of the additive on the aggregation of the target protein is independent and different from that of the chaperone)...
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Tài liệu Báo cáo khoa học: Branched N-glycans regulate the biological functions of integrins and cadherins doc
... the upregulation of GnT-III In fact, the results of several studies suggest that E-cadherin can induce ligand-independent activation of EGFR and subsequent activation of Rac1 and MAP kinase, which ... overexpression of GnT-III inhibited a5b1-mediated cell spreading and migration, and phosphorylation of FAK [61] The binding affinity of a5b1 for fibronectin was significantly reduced after introduction of a ... dysregulation of TGF-b1 receptor activation and signaling Loss of core fucosylation also resulted in downregulation of the EGFR-mediated signaling pathway [43] Downregulation of TGF-b receptor, EGFR and...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
... strand exchange activity of OsDmc1 by FRET Strand exchange activity of OsDmc1 Protein concentration dependence Fig Renaturation activity mediated by OsDmc1 protein in the presence of ATP and ... between donor and acceptor dyes, will therefore unveil the status of physical union and separation of complementary strands during renaturation and strand exchange, respectively, and thereby assesses ... single and double stranded DNA binding activity Binding to single stranded DNA stimulated a significant level of DNA dependent ATPase activity OsDmc1 also showed renaturation of complementary strands...
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Tài liệu Báo cáo khoa học: Oxidized elafin and trappin poorly inhibit the elastolytic activity of neutrophil elastase and proteinase 3 pdf
... of increasing concentrations of native and oxidized elafin and trappin on the activity of a constant concentration of NE and kdiss Ki ¼ kdiss ⁄ kass [5,8] Trappin is a 9.9-kDa protein formed of ... of elafin and trappin oxidation on the affinity of the inhibitors for NE is caused by 5886 Elafin and the inhibitory domain of trappin each have two methionine residues (M25 and M51 for elafin, and ... results of competition experiments carried out with 5887 Inhibition of elastase and proteinase by elafin S M Nobar et al Table Theoretical and observed inhibition of the elastolytic activity of neutrophil...
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