analyzing temperature induced transitions in disordered proteins by nmr spectroscopy and secondary chemical shift analyses
DEVELOPING SOFTWARE TOOLS FOR STRUCTURE DETERMINATION OF LARGE PROTEINS BY NMR SPECTROSCOPY
Ngày tải lên :
04/10/2015, 15:45
... structural and dynamic variables and in principle contains a wealth of information on the state of the system under investigation 1.2 Spin-Spin Coupling Spin-active nuclei separated by three chemical ... molecule Spin-spin coupling has been extensively exploited in one dimensional (1D) NMR experiment to determine the structures of small organic compounds In proteins, spin-spin coupling opens a ... identifying possible NOE peaks for each of the spin triplets based on the imported chemical shifts, and finally resolving ambiguities by considering both intraresidue and sequential NOEs, and the...
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The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy
Ngày tải lên :
14/09/2015, 14:25
... C-terminus domain, to expose binding sites for Src-homology-2 (SH2) domain-containing proteins and phosphotyrosine-binding (PTB) domain-containing proteins Adaptor proteins with a SH2-binding domain such ... epitope tag heparin-binding epidermal growth factor protein kinase C-related kinase homology region IQ motif containing GTPase activating protein infrared insulin receptor tyrosine kinase substrate ... protein kinases, actin-associated proteins and adaptor proteins (65) Many of the effectors contain a conserved 18 amino-acid binding motif which is named CRIB (for Cdc42-Rac interactive binding)...
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Tài liệu Contagion in financial networks by Prasanna Gai and Sujit Kapadia pdf
Ngày tải lên :
17/02/2014, 21:20
... de ne a second single-argument generating function, G y/, for the number of links leaving a bank reached by following a randomly chosen incoming link Because we are interested in the propagation ... has two degrees, an in- degree, the number of links that point into the node, and an out-degree, which is the number pointing out Incoming links to a node or bank re ect the interbank assets/exposures ... the total interbank asset position of every bank is evenly distributed over each of its incoming links and is independent of the number of links the bank has (if a bank has no incoming links, AiI...
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Báo cáo khoa học: A simple protocol to study blue copper proteins by NMR pot
Ngày tải lên :
08/03/2014, 08:20
... Westler, W.M (1998) NMR investigations of Clostridium pasteurianum rubredoxin Origin of hyperfine 1H, 2H, 13C and 15N NMR chemical shifts in iron-sulfur proteins as determined by comparison of experimental ... Determination of the magnetic axes of cobalt (II) and nickel (II) azurins from 1H NMR data: in uence of the metal and axial ligands on the origin of magnetic anisotropy in blue copper proteins ... reduction potential, and geometry Inorg Chim Acta 243, 67–78 Werst, M.M., Davoust, E.E & Hoffman, B.M (1991) Ligand spin densities in blue copper proteins by Q-band 1H and 14N ENDOR spectroscopy J Am...
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Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc
Ngày tải lên :
08/03/2014, 08:20
... containing inactive versions of Rz-1 and Rz-2 were obtained in the same way by using templates as described above but in which the guanosine nucleotides, marked in bold, had been replaced by adenosine ... gene silencing RNAi is a valuable therapeutic tool for drug design and operates in the cytoplasm It is induced by small interfering RNAs (siRNAs), which are the products of a double-stranded RNA ... plasmids monitored by RT/PCR Plasmids encoding Rz-1, Rz-2 and their inactive versions Rz-1i and Rz-2i were used for cell transfection As controls, only lipofectin (NO) or lipofectin and an empty plasmid...
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
Ngày tải lên :
16/03/2014, 18:20
... loading mix containing 2% (w/v) SDS and heated for at 90 °C The reaction mixtures containing hCypA were clarified by ultracentrifugation (100 000 g, h) and stored at °C NMR spectroscopy All NMR ... isotopic dilution and thereby improved labelling efficiency and enhanced sensitivity of the NMR analysis In the absence of enzyme inhibitors, increased yields may be obtained by providing the rapidly ... Escherichia coli by in- cell NMR spectroscopy J Am Chem Soc 123, 8895–8901 Gronenborn, A.M & Clore, G.M (1996) Rapid screening for structural integrity of expressed proteins by heteronuclear NMR spectroscopy...
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Charles Lever, His Life in His Letters, by Edmund Downey and Charles James Lever1Charles Lever, His Life in His Letters, by Edmund Downey and Charles James LeverThe Project Gutenberg EBook of Charles Lever, His Life in His Letters, Vol. I (of II), by docx
Ngày tải lên :
23/03/2014, 05:20
... spent in relating it In a few minutes the room was cleared, the bystanders were drinking their coffee and enjoying their meerschaums, scattered through the gardens; and I returned to my lodgings ... finding his hand becoming unsteady, he deliberately lowered his arm to his side, stiffening and stretching it to its utmost length, and remaining thus for an instant, he appeared to be summoning ... chance and after dinner for invention; and last, not least, to the moneyed stimulus for material "I have been exceedingly gay dining at the Embassy and elsewhere, and thinking of everything save...
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Báo cáo khoa học: Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli docx
Ngày tải lên :
23/03/2014, 18:20
... PaaZ, unknown, putative ring-cleavage enzyme, aldehyde dehydrogenase domain (N-terminus), MaoC-like protein domain (C-terminus) PaaX and PaaY may be involved in regulation (Andover, MA, USA) Oligonucleotides ... photometrically (260 nm) and by online liquidscintillation counting The retention times for phenylacetate, phenylacetyl-CoA and 2-hydroxyphenylacetate were 20, 21 and 12 min, respectively NMR spectroscopy ... mixtures containing 0.1 M phosphate, pH 6.5, 40 mg L-[U-13C9]phenylalanine or L-[ring-13C6]phenylalanine, 740 kBq [U-14C9]phenylalanine, and U L-amino acid oxidase (Fluka, Neu-Ulm, Germany) in a total...
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Báo cáo khoa học: Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling pdf
Ngày tải lên :
30/03/2014, 02:20
... structural domain constituting the Ca2+ binding motif is found in a large number of Ca2+-binding proteins, and is also known as the EF hand [16] Proteins containing the EF hand are divided into two ... equilibrium in the Qy state As shown in the inset to Fig 2A, the spectra shift slowly to blue against a temperature increase below 293 K, and the shift is faster and shows linear temperature ... maximum (fwhm)] on raising the temperature from 273 to 323 K The increase in spectral shift or bandwidth in response to the increase in temperature may indicate the involvement of more thermally...
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RECENT APPLICATION IN BIOMETRICS Edited by Jucheng Yang and Norman Poh potx
Ngày tải lên :
28/06/2014, 14:20
... combination of hand geometry and palmprint using only low cost devices for medium security environments The device uses infrared illumination and infrared camera in order to handle changing lighting ... Shuo Wang and Jing Liu Department of Biomedical Engineering, School of Medicine, Tsinghua University P R China Introduction In an era of information technology, mobile phones are more and more ... classified into the imaging type, mechanical type and electrical type The imaging type includes, but is not limited to the recognition of face, teeth and palm print in addition to fingerprint and iris,...
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STATE OF THE ART IN BIOMETRICS Edited by Jucheng Yang and Loris Nanni pdf
Ngày tải lên :
28/06/2014, 14:20
... Fingerprint Quality Analysis and Estimation for Fingerprint Matching Shan Juan Xie, JuCheng Yang, Dong Sun Park, Sook Yoon and Jinwook Shin Chapter Fingerprint Matching using A Hybrid Shape and ... concerning the Thin Plate Spline (T.P.S) and shape context will initially be established Following this, a fingerprint matching algorithm using Fingerprint Matching using AOrientation Descriptor and ... definition in the ridges on the pads of the fingers Purdue University has made several inquiries into the image quality of fingerprints and fingerprint recognition sensors involving elderly fingerprints...
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The Project Gutenberg EBook of Evolution in Modern Thought, by Ernst Haeckel and J. Arthur Thomson ppt
Ngày tải lên :
28/06/2014, 19:20
... it is, introducing order into masses of uncorrelated facts, interpreting enigmas both of structure and function, both bodily and mental, and, best of all, stimulating and guiding further investigation ... lock refused In a scholarly, critical, and pre-eminently fair-minded way, admitting difficulties and removing them, foreseeing objections and forestalling them, he showed that the doctrine of descent ... how intermediate species may once have linked those that now seem discontinuous "All natural orders of beings present but a single chain" "All advances by degrees in Nature, and nothing by leaps."...
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Báo cáo khoa học: "Intrinsic disorder in Viral Proteins Genome-Linked: experimental and predictive analyses" pot
Ngày tải lên :
12/08/2014, 04:21
... initiation factor 4E and the poly(A)-binding protein in planta Journal of General Virology 2004, 85:1055-1063 Beauchemin C, Laliberte J: The Poly(A) Binding Protein Is Internalized in Virus -Induced ... RYMV and LMV VPgs contain both intrinsically disordered domains but with different disordered states Using in silico analyses, ID domains were predicted to occur in 14 VPgs of sobemo-, poty -and ... predicted disordered regions (PDRs) is found in protein domains involved in protein-protein transient interactions (signalling and regulation) So far, disorder prediction data for viral proteins are...
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Báo cáo y học: " Distribution of hepatitis C virus genotypes in patients infected by different sources and its correlation with clinical and virological parameters: a preliminary study" pptx
Ngày tải lên :
13/08/2014, 13:20
... places of infection of the patients and genotype However, genotype was found only in north and west of country and mixed infection with genotype 1a and 1b only in center In this study, the dominant ... center In this study, the dominant genotype(s) in different regions of Iran consist: 1a, 1b and 3a in center and west, 1a and 3a in north and 1a in south and east (table 2) The geographic distribution ... results in drawing blood from cuts (less than 100 cc) It is usually done by a non-physician, using non-standard instruments (done for healing or cure purposes) It is also named "cupping" and related...
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Báo cáo y học: "Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling" doc
Ngày tải lên :
14/08/2014, 08:21
... Asparagine Threonine Methionine Isoleucine Thymine Pyrimidine Uracil Cis-aconitate Isocitrate α - Ketoglutarate Glutamate family Glutamate Glutamine Arginine Polyamines Proline 5-methyl -thioadenosine ... the protein samples obtained from the outer membrane of the ancestor and evolved lines showing the identified proteins by peptide mass fingerprinting lier study of adaptation of Ralstonia in selective ... diversion of key intermediates to biosynthetic pathways In the box and whisker plots, and represent MG and DH lines (ancestors), and and represent MGAdp and DHAdp lines (evolved) The top and bottom...
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Quantification of epidermal growth factor receptor dynamics and interactions in living cells by fluorescent correlation and cross correlation spectroscopy
Ngày tải lên :
10/09/2015, 09:25
... methods for studying rafts is interrupting rafts by changing the amount of cholesterol in cells, which includes increasing its amount by adding in extra cholesterol and extracting it by drug treatments ... (erlotinib, efitinib, and Gifitinib, for example), target to the ATP-binding pockets within the EGFR tyrosine kinase (TK) domain and block the phosphorylation and the following signalling 3) ... effector proteins via Src homology (SH2) and phosphotyrosine binding (PTB) domains After activation by its ligands, EGFR is internalized and degraded quickly, which is referred to as EGF-induced...
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Mapping the binding site of ligands to proteins using chemical exchange parameters by NMR spectroscopy
Ngày tải lên :
14/09/2015, 08:49
... determined by fitting of chemical shifts to model equations for hBclXL and BH3I-1 system 38 4.1 The parameters obtained by line shape analysis for binding and nonbinding ... 78 Bibliography 95 ix Summary Mapping the binding site of ligands to proteins using chemical exchange parameters by NMR Spectroscopy NMR spectroscopy, along with X-ray crystallography, ... pro-apoptotic proteins are classified into two types, namely, ‘Bax like’ proteins and ‘BH3 only proteins The ‘Bax like’ proteins contain three BH domains namely, BH1 through BH3, but lack the BH4 domain...
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Báo cáo khoa học: Structures and mode of membrane interaction of a short a helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy pdf
Ngày tải lên :
17/03/2014, 11:20
... structures of proteins by simulated annealing with interproton distance restraints Application to crambin, potato carboxypeptidase inhibitor and barley serine proteinase inhibitor Protein Eng 2, ... fluorescence and quenching experiments To determine the environment and the depth of penetration of the peptides, changes in the intrinsic Trp fluorescence were measured in NaCl/Pi and upon membrane binding ... both the blue shift in tryptophan fluorescence observed following membrane binding, and the quenching of Trp fluorescence by brominated lipids, indicate that the all-L-residue peptide and its diastereomer...
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DISCRIMINATION OF COLOR COPIER/LASER PRINTER TONERS BY RAMAN SPECTROSCOPY AND SUBSEQUENT CHEMOMETRIC ANALYSIS
Ngày tải lên :
24/08/2014, 11:52
... samples being obtained from Original Equipment Manufacturer (OEM) printers The toner was sampled by obtaining printouts from various printers The printouts were assigned to the printer make and model; ... commonly used and accomplishes its task by using a low order polynomial to fit to the points in a given window These methods all require a window width, where the points inside the window are considered ... evidence since it was first introduced into the forensic discipline, including accelerants, inks, fibers, document examination, ammunition, gun powder, glass and paint.32 As just mentioned, forensic...
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DISCRIMINATION OF COLOR COPIER/LASER PRINTER TONERS BY RAMAN SPECTROSCOPY AND SUBSEQUENT CHEMOMETRIC ANALYSIS
Ngày tải lên :
24/08/2014, 12:40
... contains multiple domains, each with a different function The N-domain, coiled-coil domain, linker domain, DNA binding domain, SH2 domain, and carboxy terminal transcriptional activation domain ... signalling One group of proteins known to suppress all STATs is the suppressors of cytokine signaling (SOCS) SOCS can inhibit STAT3 signaling by interacting with the cytokine receptors, inhibiting ... SH2 domain to bind a cytokine receptor through studies involving the deletion of α helixes and [56] The DNA binding domain appears similar to an immunoglobulin, with two long β strands running perpendicular...
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