analytical methods and regulations

... severaland step series of reactions involvingring opening and amide formation with pyrrolidine,oxidationtothealdehyde,epimerization,reduction ,and ether formation ledto amide [34] Ozonolysis and ... theunexpectedloss of tosylate and aziridine formation Olefin formation from [56] via the N-oxide and chain extensiongaveacid [57J Iodolactonization and tri-n-butyltin hydride reduction in thestandard fashion ... protectionof the primaryand secondary hydroxyl groups as a tosylate and ethoxyethyl ether, respectively and reaction with lithium dibutylcuprate.Treatment of [l061 with acid ledtodeprotectionand lactonization...

Ngày tải lên: 17/02/2014, 05:20

Ngày tải lên: 23/03/2014, 17:20

... STEREOCHEMISTRY: ANALYTICAL METHODS AND PHARMACOLOGY h h h h FIGURE 1.2 Paratartrate of soda and ammonia formed by an equal mixture of hemihedral crystals of levo-tartrate (on left) and dextro-tartrate ... using a hand lens and pair of tweezers, laboriously separated a quantity of the sodium ammonium salt of paratartaric acid into two piles, one of left-handed crystals and the other of right-handed ... [16/3/012/17:30:21] [17–29] 18 DRUG STEREOCHEMISTRY: ANALYTICAL METHODS AND PHARMACOLOGY FIGURE 2.1 Examples of symmetrical and dissymmetrical letters in the Latin alphabet and their mirror images FIGURE 2.2 An...

Ngày tải lên: 16/06/2017, 14:51

... refer to variables by the first part name and the question mark Thus, if we type a command using GDP? EViews uses all three GDP series for the USA, Canada, and Mexico Notice the button PoolGenr PoolGenr ... cross-section weights or SUR methods or any AR(p) specification is used, the results all asymptotically based so that the t-stats are approximately standard normal and the Wald F-stats are approximately ... coefficients and intercepts Even though you may fail to reject the hypotheses that represent restrictions that you impose, the hypotheses may not be perfectly true, and that may affect the estimators and...

Ngày tải lên: 20/12/2013, 18:15

... standard (CEN) methods Scope and format of CEN methods CEN requirements for widely accepted multi-matrix/multi-residue methods Requirements for (newer) methods with limited scope Assessment and ... Introduction Residue analytical methods for plant materials Nature of the residue Analytical method Residue analytical methods for soil Nature of the residues Analytical method Analytical methodology ... in the 1950s and 1960s to include legal limits (tolerances) for residues on foods and in feeds and, with time, in water and air.2 Enforcing these regulations required analytical methods of ever-increasing...

Ngày tải lên: 17/03/2014, 02:20

... part-types A and B (compared to simulation) obtained from the CMT and CD methods 3.4 34 Errors in the estimates of production rates of part-types A and B obtained from the CMT and CD methods for ... Mohan Gunasekaran, Chen Nutan and Yu Deping My heartfelt thanks to my friends Rajika Wimalasena and Tharushi Victoria, and relatives Damayanthi, Jeffrey and Suranthi Fernando, for making life without ... friends and staff at Control and Mechatronics Lab I and II, especially my colleagues, Cao Yongxin, Chen Ruifeng, and Lin Yuheng who were selfless in lending their support, both emotional and technical...

Ngày tải lên: 09/09/2015, 18:59

... Aspects and Analytical Methods 205 Participating laboratories were: Department of Analytical Chemistry of L’Oréal, Department of Analytical Chemistry of University of Valencia and the IRMMAnalytical ... running buffer Colouring Agents in Decorative and other Cosmetics Analytical Methods a Sample is diluted with water and filtered 2:59 PM Wang and Huang (2005) Analytical techniqueb 1/10/2007 Target ... Zviak and Milléquant, 2005a) 4.3 Hair Dyes Regulatory Aspects and Analytical Methods Table 4.3.1 193 Ch004.qxd 1/10/2007 2:59 PM 194 Page 194 Colouring Agents in Decorative and other Cosmetics Analytical...

Ngày tải lên: 24/11/2016, 18:08

... Red Nos 21 and 22 (Weisz et al., 2004), and hexachlorobenzene (HCB) and 2-bromo-3,4,5,6-tetrachloroaniline (2BTCA) in D&C Red Nos 27 and 28 (Andrzejewski and Weisz, 1999 and Weisz and Andrzejewski, ... Weisz A., D Andrzejewski, H M Fales and A Mandelbaum, 2001, J Mass Spectrom 36, 1024 Weisz A., D Andrzejewski, H M Fales and A Mandelbaum, 2002, J Mass Spectrom 37, 1025 Weisz A., D Andrzejewski, ... al., 1985), and several azo-acid, direct, and disperse dyes (Freeman and Williard, Ch004.qxd 1/10/2007 174 2:59 PM Page 174 Colouring Agents in Decorative and other Cosmetics Analytical Methods Figure...

Ngày tải lên: 24/11/2016, 18:09

... a UV monitor and collect fractions using a fraction collector and subject to the appropriate carbohydrate and antibody analyses 3.6 Analysis of Mucins Methods for the detection and analysis of ... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linked immunosorbent assays (ELISA) All these techniques have advantages and disadvantages Solution methods ... three principally different methods are available: solution assays such as colorimetric assays for hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic...

Ngày tải lên: 08/10/2012, 10:25

... mammary gland, defined by antibodies to cytokeratins J Cell Sci 75, 17–33 Kiernan, J A (1990) Histological and Histochemical Methods: Theory and Practice, Pergamon, Oxford, UK Andrew, S M and Jasani, ... M and Milton, J D (1986) Lectin Methods and Protocols, Humana, Totowa, NJ Liener, I E., Sharon, N., and Goldstein, I J (1998) The Lectins: Properties, Functions, and Applications in Biology and ... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory and Practice (45) provide...

Ngày tải lên: 08/10/2012, 10:25

... core protein specificity), and lower sensitivity than slot-blot and immunoassay methods Chap- Detection and Quantitation of Mucins 47 ter (2) discusses these assays and they are mentioned here ... greater sample volume, and superior washing) For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samples should ... NJ Nagma, K., Thornton, D J., Khan, N., and Sheehan, J K (2000) Separation and identification of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T.,...

Ngày tải lên: 08/10/2012, 10:25

... and held together by bolts tightened by hand Millipore filter paper to act as a backing for the mucosa during sectioning and subsequent manipulation of the section 2.2 Histological Staining and ... (w/v) poly-L-lysine for and dried at room temperature Methods 3.1 Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections (see Notes 1–4) This is a rapid and simple method for ... layer will be greatly reduced and discontinuous Careful handling of the cryostat sections on the slide during fixing and staining is important because excessive washing and so on can remove mucus...

Ngày tải lên: 08/10/2012, 10:25

... PhosphorImager The identification of mucin precursors and mature mucins using polypeptide-specific antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several references (1,4–11) The precursor ... Strous, G J and Dekker, J (1992) Mucin-type glycoproteins Crit Rev Biochem Mol Biol 27, 57–92 Van Klinken, B J W., Dekker, J., Büller, H A., and Einerhand, A W C (1995) Mucin gene structure and expression ... Einerhand, A W C., and Dekker, J (1995) Biosynthesis of intestinal mucins: MUC1, MUC2, MUC3, and more Biochem Soc Trans 23, 814–818 11 Van Klinken, B J W., Einerhand, A W C., Büller, H A., and Dekker,...

Ngày tải lên: 08/10/2012, 10:25

... a small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer Leave to cool before pouring (hand hot) and insert well-forming comb (see ... periodic acid-Schiff (PAS) assay and with a variety of lectins and mucin- or carbohydrate-specific antibodies (for a discussion of the relative merits and drawbacks of these analytical tools, see Chapter ... are 1.5 mm thick and cm wide There is a compromise between band broadness and amount of sample to be loaded Larger amounts of sample tend to yield poorer quality data; i.e., the bands become more...

Ngày tải lên: 08/10/2012, 10:25

... Kadler, K E., and Chapman J A (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing J Ultrastruct Res 91, 66–76 14 Kawahara K and Tanford C (1966) Viscosity and density ... (Fullerton, CA) SW 40Ti swing-out rotor, and finally unloading and analysis of the tube contents Measure out 18 mL of M GuHCl into one chamber of the gradient maker and an equal volume of M GuHCl into ... into 0.5-mL fractions, and these were analyzed for reactivity with antisera for the MUC5AC (᭹) and MUC5B (᭺) mucins The MUC5B mucin is more polydisperse than the MUC5AC mucin and has molecules of...

Ngày tải lên: 08/10/2012, 10:25

... 114 Yan and Packer chromatography, and leads to a significantly lower recovery of all the amino acids, especially serine and threonine Strong acid, high temperature, and short time acid ... at least for pump and column equilibration with the initial composition of the mobile phase Figures and show typical chromatograms of the separation of amino acid standards and hydrolyzed bovine ... samples and Dr Andrew Gooley and Prof Keith Williams for their support References Yan, J X., Wilkins, M R., Ou, K., Gooley, A A., Williams, K L., Sanchez, J.-C., Golaz, O., Pasquali, C., and Hochstrasser,...

Ngày tải lên: 08/10/2012, 10:25

... Notes and 8) and the length of sequence obtained is dependent on the number and distribution of proline residues Proline cleaves with reduced efficiency in Edman sequencing (Fig 1, vessel 1), and ... I., Kirk, M., Barnes, S., Lowary, T L., and Hart, G W (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by β-elimination and tandem electrospray mass spectrometry Anal ... Membranes (see Note 1) Reagents are available in kit form from Beckman and PerSeptive Biosystems (MA; see Note 2) Milli Q water and analytical grade methanol are required for wash steps 2.4 Edman Degradation...

Ngày tải lên: 08/10/2012, 10:25

... tandem repeat domain and overlapping each other by six amino acids (16), and the other used octamers overlapping each other by seven amino acids (17) Twelve antibodies were analyzed in total and ... information on antibody specificity and permits identification of significant features of an epitope that may contribute to antibody recognition and binding (see Notes 10 and 11) 1.3 Purification of ... must be pure and free from amines Several methods may be used to purify DMF (see Note 16) Piperidine 20% v/v: used for Fmoc deprotection Piperidine should be redistilled before use and made up...

Ngày tải lên: 08/10/2012, 10:25

... lysates and media Nonspecific bands may be present and can be eliminated from consideration using the pSVL vector control lanes for comparison (Fig 2A,B, lanes and 3) The sizes of specific bands ... polymerase buffer, 2.5 mM MgCl2, 400 µM dNTPs, 200 ng of primers and or and 4, ng of template DNA (with only primers and 4), and U of Taq polymerase Perform a PCR program in a suitable thermal ... polymerase, and incubate the reaction at 72˚C to allow extension of the linked templates Add µg each of primers and 6, and continue the PCR with 30 cycles at 94˚C for 30 s, 50˚C for and 72˚C for...

Ngày tải lên: 08/10/2012, 10:25

... gas Program the oven temperature from 150 to 230°C and 3°C/min and then 230 to 250°C at 5°C/min Use injector and detector temperatures of 240 and 250°C, respectively Use an injection volume of ... Nakamura, M., and Ohkura, Y (1987) Fluorometric high performance liquid chromatography of N-acetyl- and N-glycolyl neuraminic acids and its application to their microdetermination in human and animal ... monosaccharides, hexosamines, and uronic acids (see Subheading 3.3.3.2.) a Eluent 1: Deionized water b Eluent 2: 25 mM NaOH and 0.25 mM sodium acetate c Eluent 3: 200 mM NaOH and 300 mM sodium acetate...

Ngày tải lên: 08/10/2012, 10:25