analysis of receptor ligand interactions by surface plasmon resonance

... J: In situ sensing of single binding events by localized surface plasmon resonance Nano Lett 2008, 8:3450-3455 11 Scarano S, Mascini M, Turner AP, Minunni M: Surface plasmon resonance imaging ... http://www.translational-medicine.com/content/9/1/85 Page of Figure Specificity of the detection with SPR biosensor A) Hybridization of PCR products of S aureus A total of 50 nM of the PCR products of S aureus were incubated ... laboratory detection of bacterial meningitis Am J Med 1983, 75:119-123 Nakatani K, Sando S, Saito I: Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance Nat...

Ngày tải lên: 18/06/2014, 19:20

... J: In situ sensing of single binding events by localized surface plasmon resonance Nano Lett 2008, 8:3450-3455 11 Scarano S, Mascini M, Turner AP, Minunni M: Surface plasmon resonance imaging ... http://www.translational-medicine.com/content/9/1/85 Page of Figure Specificity of the detection with SPR biosensor A) Hybridization of PCR products of S aureus A total of 50 nM of the PCR products of S aureus were incubated ... laboratory detection of bacterial meningitis Am J Med 1983, 75:119-123 Nakatani K, Sando S, Saito I: Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance Nat...

Ngày tải lên: 20/06/2014, 03:20

... internally reflecting base of the prism to the bound surface field of the surface plasmons Experimentally, the resonance is observed by monitoring the reflected light as a function of the incident angle ... Gratings fabricated by these two methods could be used for the surface plasmon coupling The process of PANI / SPANI multilayer film manipulated on the Au surface by the Layer -by- Layer technique ... the excitation of surface plasmons in SPS Fig 3.3 Comparison of angular and kinetic scans during adsorption of additional layer Fig 3.4 Sketch of the cell in EC-SPS Fig 3.5 Sketch of an evaporation...

Ngày tải lên: 08/11/2015, 17:00

... effect of various classes of ligands on this equilibrium Ligand modulation of HD–CoRNR ternary complexes In order to monitor the effect of different types of ligands on the recruitment of two ... corepressor–HD interaction Effects of RAR ligand binding on the stability of the HD– CoRNR2 complex ESI mass spectra obtained after the addition of a twofold molar excess of different ligands and CoRNR2 are ... induces some stabilization of the HD–CoRNR1 complex (51% in the presence of HX531 vs 43% in the absence of any ligand; Table 1) Because of the presence of a second ligand molecule bound to each...

Ngày tải lên: 23/03/2014, 13:20

... areas) Correlation coefficients (r2) of standard curves were > 0.98 Binding assays were performed by analysis of preincubated mixtures of various concentrations of the peptides (including a marker ... amount of LMW heparin or water (controls) Thus, the differences in the positions of the fitted straight lines are indicative of the amount of bound peptide It is clear that binding of heparin by the ... AP-1), the data cover only a limited part of the binding curve because of peptide solubility problems and because of the limits of detection offered by UV-based detection in CE The data therefore...

Ngày tải lên: 31/03/2014, 23:20

... 12 Hutter E, Fendler JH: Exploitation of localized surface plasmon resonance Adv Mater 2004, 16:1685 13 Willets KA, Van Duyne RP: Localized surface plasmon resonance spectroscopy and sensing Annu ... size-selective detection of proteins using a chip-based and heteroliganded gold nanoisland by localized surface plasmon resonance spectroscopy Nanoscale Research Letters 2011 6:336 Page of Submit your ... Schematic view of a heteroliganded gold nanoisland for the sensitive and molecular size-selective detection of a protein The enlarged schematic diagram shows the surface morphology of a heteroliganded...

Ngày tải lên: 21/06/2014, 04:20

... confined to the investigation of some discourse features of graduation speeches The scope of the study is just limited to the analysis of formal speeches delivered by guest speakers at graduation ... BACKGROUND 2.2.1 Text 2.2.1.1 Concept of Text 2.2.1.2 Features of Text 2.2.2 Discourse & Discourse Analysis 2.2.2.1 Concept of Discourse 2.2.2.2 Concept of Discourse Analysis 2.2.3 Written and Spoken ... The focus of the study is on the discourse analysis of EGSs so the concept of discourse analysis, text, cohesion, and coherence are included in this chapter I have chosen the viewpoints of some...

Ngày tải lên: 26/11/2013, 13:27

... The error analysis supplanted Contrastive Analysis and became a recognized part of Applied Linguistics owing to the work of Corder (1967) who saw it from a different point of view Error analysis ... valuable aspects of contrastive analysis have been incorporated into the study of language transfer, it was unable to predict a great majority of errors 28 A key finding of error analysis has been ... error analysis, errors in language learning are not caused by the interference of learners’ mother tongue but by learners’ learning strategies Corder proposes that the process of error analysis...

Ngày tải lên: 18/12/2013, 10:04

... AN ANALYSIS OF THE INAUGURAL ADDRESS BY G.W BUSH IN THE U.S PRESIDENTIAL ELECTION 2004 FROM A PERSPECTIVE OF D.A VINH, 2007 VINH UNIVERSITY DEPARTMENT OF FOREIGN LANGUAGES AN ANALYSIS OF THE ... of language but the interpretation of that use needs non-linguistic features of the discourse 1.1.3.3 Features of context The best known overview of the context of situation is perhaps that of ... communism came years of relative quiet, years of repose, years of sabbatical - and then there came a day of fire (5) Nguyen Thi Huyen Le Vinh Uni 37 AN ANALYSIS OF THE INAUGURAL ADDRESS BY G.W BUSH IN...

Ngày tải lên: 18/12/2013, 10:08

... distribution of the size of the inclusions was noticed Moreover, the percentage of cells presenting inclusions as well as the distribution of the size of the inclusions were dependent on when the analysis ... percentage of the cell population with an FL2-H fluorescence greater than · 101 was recorded during the FACS analysis The percentage of decrease of DYm was calculated as the ratio of the percentage of ... stimulate some of its activities because of better substrate availability is not yet known The partial restoration of proteasome activities by Hsps contrasts with the lack of effect induced by NAC Hence,...

Ngày tải lên: 19/02/2014, 06:20

... involved identification of interaction-deficient mutants as determined by loss of the His3+ phenotype and loss of activation of the lacZ reporter gene Subsequently, clones showing loss of interaction were ... not to GST alone, which further confirms the specificity of this interaction (Fig 2B) Analysis of TIPRL protein expression by immunoblot analysis identified similar levels in the immortalized cell ... detected by immunoblot analysis TIPRL was detected in association with GST–PP2Ac but not with GST alone (C) Analysis of TIPRL subcellular distribution HEK293 cells were treated with 50 nM of the...

Ngày tải lên: 07/03/2014, 05:20

... conditions by different methods varied by as much as two orders of magnitude [12,13,17] We therefore decided to investigate membrane binding by surface plasmon resonance With this method the kinetics of ... Characterization of the surface generated by liposome binding to the modified dextran matrix of a surface plasmon resonance sensor chip An Biochem 280, 29–35 Abbott, A.J & Nelsestuen, G.L (1987) Association of ... AB koff;1 kon;2 AB þ B )* AB2 koff;2 ð2Þ ð3Þ where d½BŠ=dt ¼ À 2kon;1 ½AŠ½BŠ þ koff;1 ½ABŠ À kon;2 ½ABŠ[B] þ 2koff;2 ½AB2 Š ð4Þ d½ABŠ=dt ¼ 2kon;1 ½AŠ½BŠ À koff;1 ½ABŠ À kon;2 ½ABŠ[B] þ 2koff;2...

Ngày tải lên: 08/03/2014, 23:20

... between the two typesof people is necessary, whether either one realizes it, for the world to function The "Astronomer's Wife" is an excellent short story that brings out the often forgottenpoint ... eachother's survival While Mrs Ames perhaps could never get along without her husband, itwas no fault of her own that she didn't She provided a comfortable existance for the astronomer so that he would ... happier if Mr Ames recognized all that she had done, and had considered her lifestyle avalid one Of course an understanding was never reached, because otherwise the authorwould not have been able...

Ngày tải lên: 21/03/2014, 21:56

... direction ͑c͒ FWHM of the emerging field of structure in ͑b͒ normalized by ͑a͒ as a function of groove periodicity The slits are illuminated by a 400-nm-wavelength linearly polarized light of the interaction ... other hand, the width of the groove controls the relative phase of the SPPs generated at the two edges of each groove The depth of the grooves also influences the relative phase of the SPPs For a ... wavelength which is a function of free space wavelength, metal film, and media properties Our optimized periodicity of about ␭SPP / differs from the results of far-field collimation by grooves for which...

Ngày tải lên: 06/05/2014, 08:53

... of resultant CuO nanoparticle In order to understand the peak shift for CuO nanoparticle synthesized by both solvent cases, we carried out Williamson–Hall (W–H) analysis[ 15] of the FWHM (β) of ... for a variety of reasons, such as internal electric fields within the crystal, deformation of lattice due to strain caused by imperfection and inelastic scattering of charge carriers by phonons[16-18] ... with the strain analysis using W-H plot discussed above Figure Variation of absorption coefficient of NiO nanoparticles as a function of wavelength Figure X-ray diffraction pattern of CuO nanoparticles...

Ngày tải lên: 06/05/2014, 08:55

... of resultant CuO nanoparticle In order to understand the peak shift for CuO nanoparticle synthesized by both solvent cases, we carried out Williamson–Hall (W–H) analysis[ 15] of the FWHM (β) of ... for a variety of reasons, such as internal electric fields within the crystal, deformation of lattice due to strain caused by imperfection and inelastic scattering of charge carriers by phonons[16-18] ... with the strain analysis using W-H plot discussed above Figure Variation of absorption coefficient of NiO nanoparticles as a function of wavelength Figure X-ray diffraction pattern of CuO nanoparticles...

Ngày tải lên: 06/05/2014, 08:59

... and the quantitative analysis of various components in a complex mixture [14,15] Analysis of characteristic group frequencies in a spectrum allows qualitative estimates of chemical composition ... of been extensively employed in the field of cancer research to address the problems of tumor biology [24-30] The results of our previous research have shown its advantage in discrimination of ... corroboration with the findings of Yamada et al where the analysis of secondary structure of proteins reveal increased amount of beta sheet in necrotic area of carcinoma as compared to alpha helix [24] Moreover...

Ngày tải lên: 20/06/2014, 07:20

... effect, namely, increase of IQE via emitter /plasmon coupling and increase of light extraction efficiency [LEE] by means of out-coupling of the generated photons Enhancement of IQE Figure Enhanced ... LED by mediating surface plasmon polaritons Proc SPIE 2008, 7032:70320R 24 Gifford DK, Hall DG: Emission through one of two metal electrodes of an organic light-emitting diode via surface- plasmon ... Exploitation of localized surface plasmon resonance Adv Mater 2004, 16:1685 52 Eustis S, El-Sayed MA: Why gold nanoparticles are more precious than pretty gold: nobel metal surface plasmon resonance...

Ngày tải lên: 21/06/2014, 05:20

... wet-etched surface, with the relationship of 0 xh À xh 2  2  2 0 þ yh À yh þ zh À zh ¼ te2 P0 is also on the normal to the surface expressed by Eq Based on Eqs and 8, P0 can be calculated by ,sffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi ... Continuous wet etching of gold nanostructures in the undiluted etchant a is SEM image of the sample before wet etching, which is obtained by evaporating 50 nm of gold onto the nanospheres of 175 nm in ... nanospheres in Fig were not well dispersed, by optimizing the concentration of the nanospheres by diluting it to a ratio of 1:15, better dispersion of silica nanospheres on glass substrate was...

Ngày tải lên: 22/06/2014, 00:20

... prolonged periods of time Furthermore, the complexity of the studies limits the number of agents that can be tested Because of these issues, we have focused on the development of reliable biomarker ... used for small proof -of- concept clinical trials R358 Biomarker measurement in arthritis patients originally relied on protein analysis of fluid compartments Blood sampling is often included in ... greater than that required for a clinical laboratory analysis [26], the precision is sufficient to allow detection of twofold differences Analysis of individual tissue fragments from within individual...

Ngày tải lên: 09/08/2014, 01:23