analysis of human foxp3 treg cells phenotype and function
Báo cáo y học: "ene expression analysis of human red blood cells"
Ngày tải lên :
03/11/2012, 10:58
... 1-3 - total RNA of human RBCs from different donors; B) typical electropherogram of total RNA of human RBCs Microarray analysis of RNA from human RBCs Recent proteomic studies of RBCs based on ... mechanism of globin m-RNA stability in human RBCs Further experiments are needed to understand the mechanisms and the biological meaning of these findings But gene expression profiling of human erythrocyte ... Transcript profiling of human platelets using microarray and serial analysis of gene expression Blood 2003;101:2285-2293 Weyrich AS, Lindemann S, Tolley ND et al Change in protein phenotype without...
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Báo cáo hóa học: " Quantitative expression analysis of HHV-6 cell receptor CD46 on cells of human cord blood, peripheral blood and G-CSF mobilised leukapheresis cells" docx
Ngày tải lên :
20/06/2014, 01:20
... mononuclear cells and thereby inhibit in vitro expansion of the total cell number and of BFU-e, CFU-GM, as well as CD34+ or CD33+ cells Contrariwise we could show only less HHV6 mediated inhibition of ... surface antigen Figure of CD46 MACS blood of adult donors (PB)CD34+, CD38+, CD33+ hematopoietic from G-CSFcells from cord blood cells [triangles] and molecules on cell CD34+ cells of [circles], leukapheresis ... P: Human herpesvirus (HHV-6) Antiviral Res 1996, 31:1-21 Seya T, Hara T, Matsumoto M, Akedo H: Quantitative analysis of membrane cofactor protein (MCP) of complement High expression of MCP on human...
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Báo cáo khoa học: MicroRNA-23b mediates urokinase and c-met downmodulation and a decreased migration of human hepatocellular carcinoma cells doc
Ngày tải lên :
29/03/2014, 23:20
... development and progression of several types of malignant tumor An essential role of uPA and c-met in the migration and proliferation of human hepatocellular carcinoma (HCC) cells has been assessed by functional ... lower levels of uPA and c-met proteins and mRNAs were detectable in AB2 normal cells and in the differentiated HCC cells, HepG2 and HuH6 cells (Fig 2A,C) These data Fig The uPA and c-met 3¢-UTRs, ... uPA and c-met Fig Expression determination of mature miR-23b, uPA and c-met in human normal and HCC-derived cells (A) Real-time RT-PCR detection of miR-23b, uPA and c-met mRNAs The amounts of...
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Báo cáo khoa học: Comparative analysis of the site-specific N-glycosylation of human lactoferrin produced in maize and tobacco plants pdf
Ngày tải lên :
31/03/2014, 07:20
... glycosylation of native Lf and of recombinant Lf produced in other systems including mammalian cells [24], lepidopteran cells [25], and transgenic mice [26] N-glycosylation of milk derived human lactoferrin ... RP-HPLC and eluted with a linear gradient of 0–80% acetonitrile in Fig Fractionation of tryptic digests of natural and recombinant lactoferrins by RP-HPLC Trypsin digests of hLf (A), mLf (B) and ... peptide Glycopeptide T1 MALDI-MS analysis Ó FEBS 2003 N-glycosylation of maize and tobacco lactoferrin (Eur J Biochem 270) 3239 Fig MALDI-TOF mass spectra of hLf, mLf and tLf Asn479-glycopeptides...
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báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
Ngày tải lên :
18/06/2014, 15:20
... the function of natural killer cells, B cells, T cells and myeloid DC [16] Human pDC not express lineage specific markers, but are characterized by the expression of HLA-DR, CD4, CD123, BDCA2 and ... (CD123) and the CD4 [18] Moreover, as a hallmark of pDC, these cells are BDCA2 and BDCA4 (type II C lectin)-positive and CD11c- and CD1a-negative Trypan blue exclusion and cell counting of LV and ... and non-adherent cells in culture were harvested at day and transduced by LV-VSVG-PGK at an MOI of 18 TU/ml and at a fixed concentration of × 106 cells/ ml, in RPMI 1640 After hours at 37°C, cells...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... 8-fold greater in hES cells than in EB cells and it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected ... either hES cells or adult cells (Figure 6, panel B) For miR-106b, miR-92, miR-93, miR-130a and miR-190, the difference in their expression between EB and hES cells and between EB and adult cells were...
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báo cáo hóa học:" Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations" pptx
Ngày tải lên :
20/06/2014, 00:20
... degenerated disc cells, the structure and function of the degenerated cells changed remarkably Alini et al[11] observed an increase and maintain in density of bovine NP cells and synthesis Page of 11 (page ... effect of growth factors on proliferation of human NP cells (10%FBS/F12) (n = 20) Figure Dose-response effect of growth factors on proliferation of human NP cells (10%FBS/F12) (n = 20) Cells were ... Dose-response effect of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Dose-response effect of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Cells were...
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Báo cáo hóa học: " Design and Analysis of Nanotube-Based Memory Cells" ppt
Ngày tải lên :
22/06/2014, 01:20
... nanotube-based memory cells in practical applications Fabrication of arrays of nanotube structures such as we propose in this paper is a subject of much ongoing research CNT geometric uniformity and the ability ... XO and XI are the configurations of the outer and inner tubes, respectively Then, the force applied on particle I can be derived as the first derivative of U with respect to the coordinates of ... more specific, and we quantitatively illustrate the proposed design as SRAM and DRAM cells In addition, the continuum model developed in this paper will help to study feasibility of large nanotube-based...
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báo cáo khoa học: "Adenovirus-mediated siRNA targeting Bcl-xL inhibits proliferation, reduces invasion and enhances radiosensitivity of human colorectal cancer cells" ppt
Ngày tải lên :
09/08/2014, 02:21
... overexpression of Bcl-xL is associated with radioresistance of human osteosarcoma cells In other human cancers, the association of Bcl-xL expression and radiosensitivity of tumor cells is also ... **P
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Báo cáo y học: "The potential of human regulatory T cells generated ex vivo as a treatment for lupus and other chronic inflammatory diseases" pot
Ngày tải lên :
09/08/2014, 03:24
... have been called ‘professional’ suppressor cells [5,8] In general, the properties of rodent and human CD4+ CD25+ T cells appear to be very similar In humans, 6–18% of CD4+ T cells constitutively ... naive fraction of CD4+ cells to expand and to become contact-dependent ‘professional’ regulatory T cells TGF-β also induces CD4+ and CD8+ cells that are CD25– to become Th3-like cells Although ... IL-2-activated T cells These effects include upregulation of CD25, CTLA-4 and CD40 ligand expression on CD4+ cells [14,47], and increased tumor necrosis factor-α production by both CD4+ and CD8+ cells [47]...
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Báo cáo y học: "Polarized subsets of human T-helper cells induce distinct patterns of chemokine production by normal and systemic sclerosis dermal fibroblasts" doc
Ngày tải lên :
09/08/2014, 07:20
... capacity of T cells to elicit the production of chemokines by dermal fibroblasts and, in particular, the capacity of polarized T cells (Th1 and Th2) to modulate fibroblast production of chemokines ... increases in IL-8, MCP-1 and IP-10 were observed in the presence of activated T cells However, Th1 cells were less potent inducers of IL-8 and stronger inducers of IP-10 than were Th2 cells, although ... of polarized T cell clones at a fixed ratio of T cells to fibroblasts of 10:1 Under these settings, Th1 cells induced statistically significantly higher amounts of IP-10 than did Th2 cells, and...
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Báo cáo y học: "Abnormal costimulatory phenotype and function of dendritic cells before and after the onset of severe murine lupus" pot
Ngày tải lên :
09/08/2014, 07:20
... CD11c+ CD11b+ cells) , the pDCs and the CD8α+ DCs (recognized as CD11c+ B220+ cells and CD11c+ CD8α+ cells, respectively) The large number of cells yielded a sufficiently large number of events (more ... > 0.05 ofBALB/c and B6 DCs, in terms of both percentage of positive cells (Figure 3a,b) and MFI (not shown) In the two control strains, the expression of costimulatory molecules CD80 and CD86 ... murine models for the study of human SLE CD80 and CD86 are ligands of CD28/CTLA4 and may have different functions [29]: although both CD80 and CD86 activate effector T cells, CD80 seems to be especially...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
Ngày tải lên :
09/08/2014, 14:22
... differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the limbs, ... proximity of the cells to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by ... of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
Ngày tải lên :
10/08/2014, 05:21
... conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing, LLC assisted with collection and assembly of data, data analysis and interpretation, ... phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater ... other hand, PD98059 decreased the activation of ERK both in cells treated with FN or LAM (Figure 5B and 5D) and increased the activation of Akt about 2.7-fold and 2.1fold compared to FN and LAM...
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báo cáo khoa học: " Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells" pdf
Ngày tải lên :
10/08/2014, 10:21
... Siha cells Effects of DNMT1 silencing on cell growth and proliferation Cell growth and proliferation of Hela and Siha cells were examined using MTT assay As shown in Figure 3, viabilities of Hela ... methylation of tumor suppressor genes and tumorigenesis in its early status Currently, functions and mechanisms of DNMTs in cervical cancer cells remained unclear, and whether DNMT1 and DNMT3b ... SFRP4, TSLC1 and CHFR in Hela cells and CCNA1, PTEN, SFRP4 and TSLC1 in Siha cells were analyzed Surprisingly, transfection did not affect the methylation status and mRNA expression of FHIT and PTEN...
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báo cáo khoa học: "Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications" pps
Ngày tải lên :
10/08/2014, 22:20
... Memory 7and effector T cell analyses of DC-activated immune effector cells Memory and effector T cell analyses of DC-activated immune effector cells (A) & (B) Memory and effector phenotype analysis of ... http://www.jhoonline.org/content/2/1/34 Functional(see previous DC-activated and ex vivo expanded immune effector cells Figure analyses of page) Functional analyses of DC-activated and ex vivo expanded immune effector cells EBV ... expression of IFN-γ, IL-2 and CD107a in the CD8 T cells, and to a lesser extent, the CD4 T cells (Fig 8A top: IL-2 and IFN-γ, and bottom: CD107a and IFN-γ) The antigen-specific effector function...
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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx
Ngày tải lên :
10/08/2014, 23:21
... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... and lateral sides of the inferior patella Using a 22-gauge 1-inch needle, 8.5 cm3 of ADSCs, PRP, dexamethasone and hyaluronic acid mixture was injected into the medial and the lateral sides of ... height of her meniscus cartilage on the anterior medial side of the left knee (Figures and 6) Figure MRI sagittal T2 view of the knee Pre-treatment and post-treatment MRI shows increased height of...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
Ngày tải lên :
11/08/2014, 12:21
... tri) methylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; H3K36me3, trimethylation of lysine 36 of histone H3; ... H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; ... JA, Andrews RM, Flicek P, Boyle PJ, Cao H, Carter NP, Clelland GK, Davis S, Day N, Dhami P, Dillon SC, Dorschner MO, Fiegler H: Identification and analysis of functional elements in 1% of the human...
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Báo cáo y học: " Antibody microarray analysis of cell surface antigens on CD4+ and CD8+ T cells from HIV+ individuals correlates with disease stages" ppt
Ngày tải lên :
13/08/2014, 05:22
... investigation of the causes and control of HIV disease progression and eventually lead to a better understanding of the pathogenesis of the disease Our study is the first to demonstrate how density of cell ... cell proliferation [24] DotScan analysis showed that CD4+ T cells of LTNP and VIR groups expressed significantly higher levels of expression of CD71 than that of the BDL group Consistent with ... identification of novel differential markers expressed or co-expressed on CD4+ and CD8+ T cells, which could aid in defining the stage of evolution of HIV infection and the immune status of the patient...
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Báo cáo y học: " Functional analysis of human and chimpanzee promoters" docx
Ngày tải lên :
14/08/2014, 14:21
... availability of human and chimpanzee DNA sequence of high quality, the possibility to place primers for amplification of the promoters as well as the successful amplification and cloning of the promoter ... W, Johnson RM, Hof PR, Kapatos G, Grossman LI, Goodman M: Sister grouping of chimpanzees and humans as revealed by genome-wide phylogenetic analysis of brain gene expression profiles Proc Natl ... Myers RM: Identification and functional analysis of human transcriptional promoters Genome Res 2003, 13:308-312 Buckland PR: Allele-specific gene expression differences in humans Hum Mol Genet 2004,...
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