analysis of audiovisual expression

... scintillation counting of isolated products on sections of the filter Virtual Northern blotting Semi-quantitative analysis of gene expression profiles, either by Northern blot analysis or by differential ... the intensity of the signals in each band by using appropriate software, often supplied by the manufacturer of the imaging equipment These programs allow integration of the intensity of the band ... intensity of green versus red This gives a measure of the relative levels of the competing mRNAs bound to each spot and so provides information on the relative level of expression of the genes...

Ngày tải lên: 25/10/2013, 22:20

... al (2005) Analysis of long-lived C elegans daf-2 mutants using serial analysis of gene expression Genome Res 15, 603–615 10 Ryu EJ, Angelastro JM & Greene LA (2005) Analysis of gene expression ... gene expression: rapid RT-PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 34 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression ... Serial analysis of gene expression in non-small cell lung cancer Cancer Res 58, 5690– 5694 Hashimoto S, Suzuki T, Dong HY, Nagai S, Yamazaki N & Matsushima K (1999) Serial analysis of gene expression...

Ngày tải lên: 07/03/2014, 00:20

... Serial analysis of gene expression: rapid RT-PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis ... Ciriello KM et al (1999) Analysis of human transcriptomes Nat Genet 23, 387–388 Hashimoto S, Suzuki T, Dong HY, Nagai S, Yamazaki N & Matsushima K (1999) Serial analysis of gene expression in human ... 15 16 17 18 19 20 Characterization and quantification of mRNA transcripts in ejaculated spermatozoa of fertile men by serial analysis of gene expression Hum Reprod 21, 1583–1590 Matsumura H, Reuter...

Ngày tải lên: 07/03/2014, 04:20

... consisted of μl of diluted cDNA, 0.3 μM of forward and reverse primers (1 μl each), 10 μl of the 2X QuantiTect™ SYBR® green master mix, 0.1 μl of HK-UNG (Epicentre, Madison, WI), and μl of RNase-free ... content of the tissues between ACL-X and control joints for any of the regions tested Error bars indicate standard error of the mean Values are μg of HP/mg of tissue wet weight Differential gene expression ... sample processing, acquisition of data, analysis and interpretation of data, writing of manuscript KK: animal care, sample harvesting, acquisition of data, editing of manuscript DF: animal care,...

Ngày tải lên: 20/06/2014, 00:20

... data DIFFERENTIAL EXPRESSION PROFILE EXPERIMENTS This type of experiment is very common in genetics research [19, 20] and involves comparing gene expression profiles of a set of G genes expressed ... paired-t test of hypotheses (1) with fcmin = at a FDR level of 20% The second stage of the “Two-stage FDR-CI” algorithm selects Ᏻ2 as a subset of Ᏻ1 at the prescribed FDR-CI level of 20% Stage of the ... criteria such as the eigenvalue spread in a principal components analysis (PCA) of all pairs of gene expression profiles, the ratio of betweenpopulation-variation to within-population-variation,...

Ngày tải lên: 23/06/2014, 01:20

... studies as a measure of diagnosis or clinical outcome [11] Microarray analysis of gene expression at sites of tissue damage The next generation of reports describing global gene expression patterns ... we have confirmed the increased expression of several of those overexpressed genes by using real-time PCR analysis and in a second cohort of SLE patients [3] Of great interest is the 283 Arthritis ... required to determine the relative roles of type I and type II IFN in the gene expression profile observed in SLE peripheral blood Analysis of the age of study patients, time from diagnosis, organ...

Ngày tải lên: 09/08/2014, 01:23

... the miRNA expression profiles of the BCSCs were confirmed by Q-RT-PCR Bioinformatic analysis and preliminary functional analysis of BCSC-related miRNAs Chromosome localisation, sequence analysis ... and the primary functions of the target genes Table S4MiRNAs expression profile of MCF-7 cell from Ambion (signal value ≥++) The table shows MiRNAs expression profile of MCF-7 cells detected by ... Table S2- MiRNAs microarray- based miRNAs expression profile of ESA+CD44+CD24-/low cells (signal value ≥800) The table shows the miRNAs expression profile of ESA +CD44+CD24-/low cells obtained through...

Ngày tải lên: 10/08/2014, 10:20

... imbalance Correlation analysis of BCL11B in the T-ALL group revealed that the BCL11B expression level was negatively correlated with that of BCL2L1 (Bcl-xL), although over -expression of both genes was ... validated Correlation analysis of BCL11B in the T-ALL group revealed that the BCL11B expression level was positively correlated with the relative SPP1 expression level The expression of SPP1 was significantly ... CA: Inhibition of BCL11B expression leads to apoptosis of malignant but not normal mature T cells Oncogene 2007, 26:3797-3810 Livak KJ, Schmittgen TD: Analysis of relative gene expression data...

Ngày tải lên: 10/08/2014, 22:21

... up-regulation of CD127 expression on CD8 T cells is closely associated with the downregulation of CD38 and PD-1 and the upregulation of CCR7 expression [9,11,12] All occurred in concert with resolution of ... the CD127 expression of CD8 memory T cells of CHB patients and the main viral markers of HBV infection: serum HBV DNA and HBeAg There was a strong negative correlation between CD127 expression ... support the notion that increased expression of CD127 on memory CD8 T cells is linked to successful inhibition of viraemia These results indicate measurement of CD127 expression on memory CD8 T cells...

Ngày tải lên: 12/08/2014, 01:21

... http://www.biomedcentral.com/1471-2229/10/24 Page of 14 Figure Hierarchical clustering analysis of 53 selected genes based on their gene expression patterns Analysis of expression profiles of 53 ESTs (Additional File ... assess the reproducibility of the macroarray analysis Data presented for the expression profile analysis is an average of three independent experiments Expression profiles of the stress inducible ... their expression profiles in PUSABGD72 The expression profile of individual gene in the cluster is denoted by grey line and the mean expression profile is depicted by pink line The number of genes...

Ngày tải lên: 12/08/2014, 03:21

... methods and more detailed analysis of expression of selected genes to broaden our understanding of cotton fiber development Results Isolation of RNA from dpa fiber One goal of these experiments was ... their expression has been investigated in young fiber The agreement of the known expression of these genes with expression of these genes on microarrays validated the microarray results Global analysis ... meaning of the changes of expression of large sets of genes [28] Gene ontologies are definitions of genes using a well defined species-independent vocabulary GO were not available for most of the...

Ngày tải lên: 12/08/2014, 05:20

... important Suppression of osteoblast and osteoclast precursor production, increased apoptosis of osteoblasts and osteocytes, prolongation of the lifespan of osteoclasts and apoptosis of endothelial cells ... temperature of 20°C, and humidity of 48% Food and water were provided ad libitum with a standard rodent diet The weight of the rats was followed Page of 12 before and after the implant of a prednisone ... set of criteria in which the altered expression of a gene must have at least a change of ± 1.5-fold (FC = fold change) and a Pvalue less than 0.05 These criteria resulted in the identification of...

Ngày tải lên: 12/08/2014, 14:22

... wary of accepting the results of Whitfield et al as a synchrony experiment Summary The work of Liu, Gaido and Wolfinger [1] is a statistical analysis of a well-regarded paper [2] on the pattern of ... synchronized Synchronized divisions are the sine qua non of synchrony Criteria for successful analysis of gene expression during the division cycle 12) Gene expression results should be replicated (with ... different experiments in terms of variation in the phase angles of peaking of gene expression in different individual cells Thus, given a particular variation in gene expression in different cells,...

Ngày tải lên: 13/08/2014, 23:20

... Figure Properties of the set of 893 patterns Properties of the set of 893 patterns (a) Number of patterns with a given number of probe sets associated with a score of 90% (b) Number of patterns with ... Phylogenetic analysis of one example from each family (Figure 5) led to the study of the evolution of a total of 13 genes An ancestral gene existed for each of the four families before divergence of Deuterostomia, ... functions in the analyses of other collections of gene expression data sampling multiple tissues Examination of markers of stem cell differentiation One distinctive feature of the gap method is that...

Ngày tải lên: 14/08/2014, 08:20

... artifact of hybridization interactions Figure Profile of microRNA expression in the developing mouse brain Profile of microRNA expression in the developing mouse brain (a) Relative expression ... wave of expression of sequential classes of microRNAs (Figure 3a) Adult Genome Biology 2004, 5:R68 information range of the array was from 0.1 fmoles to at least 10 fmoles, or two orders of magnitude ... differences in the apparent expression of a given microRNA could be dampened or exaggerated depending on the expression of the paralogs (Figure 2a) To determine the detection range of the microarray,...

Ngày tải lên: 14/08/2014, 14:21

... expression (13.4%) using analysis of variance (ANOVA) with a nominal significance cutoff of 0.01 (false discovery rate (FDR) = 4.12%, permutation test) and 8,643 using significance analysis of ... agreement of the measurements of expression differences in the two sources of sample material, the amplitude of expression differences measured in the autopsy samples is, on average, half of that ... time of tissue freezing, the method of freezing, and the duration of storage of the frozen brain material Prior studies have indicated that these factors have relatively small effects on gene expression...

Ngày tải lên: 14/08/2014, 16:20

... SCO4662 Normalised log(2) expression −2 −4 −6 10 20 30 40 50 60 Experiments 70 80 90 100 Figure Example1operon expression profile Example operon expression profile Expression profile across all 107 ... not necessarily in terms of absolute expression level This idea is illustrated in Figure 1, which depicts the normalized expression profile of 107 experiments for each of the four genes found ... expression _ ratio _ of _ gene _ i _ in _ experiment_j ) Median _ of _ all _ expression _ ratios _ in _ experiment_j We define the expression level of each gene in a given operon of m genes as gi...

Ngày tải lên: 14/08/2014, 16:21

... time Analysis of differential gene expression and construction of developmental profiles Differential gene expression between tumors and corresponding controls was determined using significance analysis ... about 30% of the measured genes will follow this pattern The construction of the DT takes advantage of this behavior, ordering the genes in a linear array based on their temporal pattern of expression ... and vice versa The expression of certain sets of genes seems to be mutually exclusive; if one set is active, the other set is invariably turned off Third, a limited number of patterns consistently...

Ngày tải lên: 14/08/2014, 20:22

... methodology for global analysis of transcriptomes is serial analysis of gene expression (SAGE) This sequencingbased technique generates 14-bp sequences (tags) to evaluate thousands of transcripts in ... quantitative method The frequency of tag occurrence observed in a SAGE library is a measure of the expression level of each transcript, allowing comparative analysis of two or more experimental conditions ... DV22 (1, 10) DV38 (1, 9) Figure of the differential expression of X tropicalis transcripts identified by SAGE Verification Verification of the differential expression of X tropicalis transcripts...

Ngày tải lên: 14/08/2014, 21:20

... well as critical analysis of football discourse Up to now, a lot of the researches on critical analysis of political discourse have been carried out whereas critical analysis of football discourse ... and inculcated in ways of being identities (and therefore styles) Analysis of texts…is an important aspect of ideological analysis and critique…” Regarding the relationships of language, power and ... concepts of Critical Discourse Analysis and the review of previous researches related to the research are provided 1.1 Overview of Critical Discourse Analysis (CDA) 1.1.1 The history of CDA Before...

Ngày tải lên: 28/03/2015, 08:55