... incorporation/[3H]leucine incorporation) of MG6X-TNF was compared by quantitative analysis ofthe fluorograms of [3H]myristic acid- and [3H]leucine-labeled proteins shown in the lower and upper panels of (A) Relative ... in lanes and These results clearly suggest that the specific determinant that permits the Lys residue at position is the Lys residue at position To further confirm this idea, the effect ofthe ... obtain transcripts of these cDNAs subcloned into pB vector These transcripts were purified by phenol/chloroform extraction and ethanol precipitation prior to use Subsequently, the translation...
... unlike algebraic analysis in that it produces a range of possible (although inexact) fits to the data, accounts for the effect of simultaneously varying the kinetics ofthe other steps, and is also ... so the gulf between the results ofthe original model and the set of fitted and experimental concentrations is much greater than that which separates the fitted and experimental concentrations themselves ... compared to the difference between the original and fitted model values (Table 3) The steady-state fluxes ofthe original model lie well within the range covered by the steady-state fluxes ofthe fitted...
... ω(k), the time ofthe first entry in the ε-neighbourhood ofthe set of limit points is included into the time of being outside of this neighbourhood, and the last is not larger than the time of final ... → The compactness means that from any sequence a convergent subsequence can be chosen The states ofthe system are represented by the points ofthe phase space X The reader can think of X and ... 4.17 For any k ∈ K the set ω (k) is closed and k-invariant, and the function ω (k) is upper semicontinuous in K Proof The k-invariance of ω (k) follows from the k-invariance of ω (x, k) for any x...
... of 11 ALDHhiLin-transplanted animals (Figure 2) and in four of six ALDH lo Lin - transplanted animals (data not shown) The human engraftment in the ALDH hi Lin transplanted animals was generally ... with human cell present in the spleen, lung, liver, heart, and kidney Only sporadic human cells were detected in ALDHloLin- transplanted animals and never in multiple organs ofthe same animal ... to the site of tissue damage, as verified by FACS and ALU analysis [23] Human cells were found in only one ofthe ALDHloLin- transplanted animals For each section analyzed, we found to 10 human...
... participated in the design ofthe study, in the statistical analysis and revising manuscript draft CG conceived ofthe study, and participated in its design and coordination and helped to draft the manuscript ... immunohistochemistry studies and participated in the interpretation of data and revising manuscript draft FC, FL and FD participated in the design ofthe study, interpretation of data and revision of manuscript ... [21,25] Handling of animals was performed in compliance with the guidelines of animal care set by the European Union (86/609/ EEC) and the Spanish regulations (BOE67/8509-12; BOE1201/2005) on the...
... JP conceived and designed the study, DS and VN performed and analyzed the experiments DS and JP drafted the manuscript All authors have read and approved the final version ofthe manuscript Acknowledgements ... were included in the frequency analysis In the case of amplitude analysis, the same events and data segments were used Data are expressed as means ± standard error ofthe mean (SEM) Some data ... approved by the local Institutional Animal Care and Use Committee and were consistent with the guidelines ofthe International Association for the Study of Pain, the National Institutes of Health...
... http://www.occup-med.com/content/1/1/12 the long term-exposed animals In the glands, the only significant differences were present between the 20 ppm group and the control group (p < 0.05) In the area ofthe surface epithelium, ... weighing 350–400 g were used The animals were housed under standard laboratory conditions and fed ad libitum in accordance to the rules ofthe animal welfare act and the local animal committee Exposure ... after 20 and 25 days of exposure the surface epithelium appeared atypically reorganized with an increased height ofthe epithelial cells and shortened/decreased ciliae Also, 20, 40 and 80 ppm...
... is the random family effect with mean zero and variance σ f ; Iij is the random interaction or plot effect with mean zero and variance σ I2 ; and eijk is the random residual effect with mean ... ofthe original non-transformed data and phenotypic standard deviations ofthe traits X and Y, respectively Thus, the new family covariance (transformed data) represents the contribution ofthe ... than that of mature wood [40, 41] Therefore, possible consequences ofan increase in juvenile wood proportion in the stems of future plantings are a decrease of wood mechanical properties and an...
... mislearn from their private experiments faster than they make progress in therapy with analysis ofthe transference and of scenarios of old betrayals The crisis intervention ofthe Cape Cod Model ... recreate semblances of dependency in later relationships, semblances of others' betrayal and of their own powerlessness The hypothesized shared mechanism of disorder for complex PTSD and BPD has ... underlying the experimental intervention makes it relevant to review the efficacy also for deep structural reparation ofthe mechanism of disorder The theory ofthe Cape Cod Model claims that the experimental...
... With the exception of DVU1083, which is an ortholog ofthe E coli PhoB [6], none ofthe RRs have any characterized orthologs The targets of these 29 RRs represent the transcriptional portion ofthe ... 80% ethanol, and air-dried The pellets were resuspended in 25 µl water, and quantified using the nanodrop µg each ofthe Cy3 and Cy5 labeled DNA were pooled together, and vacuum dried The pellet ... LR designed the experiments, conducted the experiments, conducted the data analysis and wrote the manuscript AM designed the experiments, conducted the data analysis and wrote the manuscript EGL...
... in the data analysis and review ofthe manuscript YN performed project planning, participated in the data analysis and helped to draft the manuscript All authors read and approved the final manuscript ... with these plasmids were verified using western blotting: with anti-Nedd4 and anti-GFP antibodies, for Nedd4-GFP; and anti-UL56 and anti-RFP antibodies, for UL56 (data not shown) Transfection and ... vesicles The unstable overlap between the localization of UL56 and that of Nedd4 suggests that the interaction ofthe two proteins is dynamic and transient Given that Nedd4 participates in many cellular...
... involved in the study design and in revising the manuscript GH has made a substantial contribution to the design and conception ofthe study, and to the interpretation of data Acknowledgements The present ... values ofthe MPAP in responders The separation between responders and nonresponders made on the basis of only two mediators (ET-1 and IL-8) may therefore support the hypothesis of a distinct mechanism, ... injury Nine out ofthe 20 animals exposed to INO at hours received a second INO challenge at hours after the onset of lung injury, with the purpose of observing whether an animal changes its response...
... more ofthe symptoms now and then or more often The score distribution for the independent variables was similar to the distribution ofthe outcomes, with the majority of children reporting the ... the study AL and SL did the analyses AL, SL, and LJV interpreted the data and wrote the paper All authors read and approved the final manuscript Competing interests The authors declare that they ... headmasters, teachers and parents who contributed, and a special thanks to the children The study was financially supported by the National Education Office, Møre and Romsdal County, and by the Central...
... performance of experimental work, analysis of mechanical data, and drafting ofthe manuscript GPO contributed to animal preparation, performance of experimental work, and analysis of mechanical and ... drafting ofthe manuscript TMG contributed to analysis of molecular biology data, and drafting ofthe manuscript NNR contributed to analysis of echocardiography, and drafting ofthe manuscript ... to animal preparation, performance of experimental work, analysis of mechanical and morphometrical data, and drafting ofthe manuscript DSO contributed to analysis of molecular biology data, and...
... is the man, the marlin, the sharks, the sea and the effort ofthe man to take the marlin offshore 2.2 The hidden part and its components There are many layers of meaning interpretation in The ... Mexico On land, the action takes place in a small village on the northern coast of Cuba, below the Tropic of Cancer and not far from the capital city of Havana Havana is the capital of Cuba and forms ... Hemingway‘s Theory of Iceberg? How is the Theory revealed in The Old Man and the Sea? What can be withdrawn from the surface ofthe story? Design ofthe study This paper is organized in the following...
... irrespective ofthe nature ofthe condensing ligands and the specific surface interaction Another important part of this thesis is the study of single T4-DNA molecules confined in rectangular-shaped nano-channels ... which the biomolecule is subjected to a tensional force The role ofthe strength ofthe stretching force is the same as the value ofthe cross-sectional diameter ofthe nano-channel, in the sense ... inside the channel and will only bend when it bounces off the wall [10] In this thesis, the channel diameters exceed the persistence length, but they are smaller than the radius of gyration of the...
... status in the handshaking stage An ACK indicates the function has completed the command is now ready to accept another command If an error occurred during the processing of this command, then the function ... important information about the device such as the supported USB version, maximum packet size, vendor and product IDs and the number of possible configurations the device can have The format ofthe ... within 500ms ofthe successful transmission ofthe previous packet • The status stage must complete within 50ms after the transmission ofthe last data packet The SetAddress command (which contains...
... were manually annotated The annotation was performed at word and phrase level, and the sentiment expressions identified in the corpus were associated to the source ofthe private-state, the target ... in the sentiment annotated corpus To measure the reliability ofthe sentiment annotations, we conducted an inter-annotator agreement trial, with two annotators This was performed based on the analysis ... collection of comments posted by the readers ofthe Portuguese newspaper Público to a series of 10 news articles covering the TV debates on the 2009 election ofthe Portuguese Parliament These took...