an important e in 2e

... layer of soft agar were transferred into each test tube, respectively After then, the mixture in each test tube was immediately transferred into the bottom rigid layers Coliphage T4 and f2 were ... were affected by the extracts, PFU would change in the short term The figure and figure indicated the inhibitive effect of the P hydropiper leaf extracts on coliphage T4 and f2 The inhibitive effect ... then these solutions were centrifuged for 15 at 8000 r/m, and the supernatants were filtered through 0.45μm membrane The filtrated liquids were preserved at ℃ Preparation of beef extract peptone...

Ngày tải lên: 05/09/2013, 09:38

... sources as indicated in Fig Expression of galA was only detected in the presence of sugar beet pectin, whereas expression of lacA was detected in the presence of L-arabinose, D-xylose, sugar beet ... detected in the presence of these compounds, but in the presence of D-xylose and xylan as well This can be explained by the structure of the polysaccharides Xylan contains single b-1,4-linked ... the b 1,4-endogalactanase encoding genes from A tubingensis and A aculeatus The similarity of the galA genes is higher between the two biseriate species, A niger and A tubingensis, than between...

Ngày tải lên: 21/02/2014, 01:21

... the covered scissile peptide bonds appear at the molecular surface and become exposed to the solvent; the exposed peptide bonds are digested by one another and the enzyme becomes inactive We ... enzymes were expressed in almost same quantities as the wild-type enzymes The expressed mutant enzymes were purified, and all of the enzymes were confirmed by SDS/ PAGE, immunoblot analysis and N-terminal ... °C The denaturation temperature indicates the stability of the structure of the protein The difference of the increments between the inactive temperature and the Tm should indicate that the mutations...

Ngày tải lên: 21/02/2014, 03:20

... several families, including serine, cysteine, aspartic and metalloproteinase inhibitors [11] Serine proteinases appears to be the largest family of proteinases, and plant serine PIs have been ... blot analyses (Fig 5A) Six T2 transgenic lines (three sense lines and three antisense lines) were constructed and employed to evaluate the disease resistance of transgenic tobacco using a standard ... The predicted genes were compared with their expressed sequence tags and cDNAs obtained from the Internet A phylogenetic tree was constructed online using the default settings of the web-based...

Ngày tải lên: 23/03/2014, 03:20

... immune system to fight off the infection completely Whereas HIV-1 IN is clearly the most medically relevant IN, and has been extensively investigated for over two decades, the enzyme encoded by ... the isolated CCD, except for the extreme tip, which is occupied by a conserved isoleucine (The presence of Glu203 in an equivalent location in the ASV IN sequence provides another exception in ... 5¢-dinucleotides and seal the gaps on both sides of the integrated viral DNA In the final product, the viral insert is flanked by the repeated stagger sequence, and begins with the conserved TG sequence...

Ngày tải lên: 29/03/2014, 23:20

... VV WR infection induced the first recombination The Neomycin resistance gene (NEOR) in the p7.5:TetOA12L:NEO plasmid was used as a transient selective marker in the presence of Geneticin G418 ... assembly, T-REx 293 cells were infected by vvtetOA12L in the presence (a, b) and the absence of Tet (c, d) In the presence of Tet, spherical IV particles were demonstrated, which evolved into ... center-compressed core in IMV particles In terms of the generation of viral membranes, A12L deficient virus introduced neither the absence of viral membrane nor unfinished or interrupted IV membranes,...

Ngày tải lên: 18/06/2014, 18:20

... between grass/ cereal and maize, which belong to the Pooideae and Panicoideae subfamilies, respectively Most major allergenic pollens from grasses, weeds and trees are derived from windpollinated ... 15 pollination in the greenhouse of the research department Acute changes in airway function (∆ of parameters) were expressed for each subject as a percentage of the value before exposure [22] ... were examined who were repeatedly exposed to maize pollen by pollinating maize during their work in a biological research department All filled in a questionnaire and underwent skin prick testing...

Ngày tải lên: 20/06/2014, 00:20

... VV WR infection induced the first recombination The Neomycin resistance gene (NEOR) in the p7.5:TetOA12L:NEO plasmid was used as a transient selective marker in the presence of Geneticin G418 ... assembly, T-REx 293 cells were infected by vvtetOA12L in the presence (a, b) and the absence of Tet (c, d) In the presence of Tet, spherical IV particles were demonstrated, which evolved into ... center-compressed core in IMV particles In terms of the generation of viral membranes, A12L deficient virus introduced neither the absence of viral membrane nor unfinished or interrupted IV membranes,...

Ngày tải lên: 20/06/2014, 01:20

... screen/ overhead projector Using PowerPoint slides is an excellent way to present clear and precise information Audio-visual aids can make a presentation more interesting and easier to understand ... really sharing this experience with the audience Keep the audience interested with intelligent recap questions asked in a dynamic tone Make the audience active participants with short but meaningful ... affected by her/ his Lang.1 controls her/ his voice to make her/ his speech effective and laudable keeps the audience alert and highly interested uses visual aids properly and effectively makes meaningful...

Ngày tải lên: 28/06/2014, 12:20

... iodide-negative cells The percentages of cells in each quadrant are indicated Each plot represents a staining pattern of cells from a single female mouse Identical profiles were observed in male ... Animal Centre of the University of Leuven The experiments were performed in mice to 10 weeks old, but in each experiment the mutant and wild-type mice were age-matched within 5-day limits The male ... gene Results Effect of treatment in vivo with depleting anti-CD25 antibodies on the development of CIA in wild-type DBA/ mice In a first set of experiments we tested the importance of Treg cells...

Ngày tải lên: 09/08/2014, 06:22

... germination After d, seed germination was recorded Seeds were considered to have germinated when radicles were mm long To assess salt tolerance, three-day old seedlings were carefully transferred ... file 6) The phylogenetic analysis revealed that DREB/CBF proteins were grouped by species, and all the CBFs were clustered together according to DREB type DREB1-type CBFs were clustered together ... Arabidopsis increased salt tolerance during both germination and early seedling growth Since over-expression of MtCBF4 enhanced drought and salt stress tolerance in transgenic Arabidopsis, we examined...

Ngày tải lên: 11/08/2014, 11:21

... antigens in these complexes After identification of these antigens, a better understanding of the immunological process in the affected joints can be achieved Since anti-citrullinated protein/peptide ... the perpetuation of RA Moreover, in contrast to Fibβ, vimentin is an intracellular antigen and therefore not expected in IC Additionally, the presence of intracellular citrullinated proteins in ... with anti-Fibβ The lower abundance of Fibβ could be due to residing fibrinogen in the synovial tissue A remarkable difference between our data and previous reports is the presence of citrullinated...

Ngày tải lên: 12/08/2014, 14:22

... the 24 hour intravenous infusions [26] An older, underpowered RCT showed no difference in outcomes between omeprazole and a combination of somatostatin and ranitidine in severe GI bleeding [27] ... hypertensive gastropathy, octreotide was found to be more effective than vasopressin and omeprazole in achieving complete bleeding control with less time and fewer blood transfusions required ... [28] Because of their effect on decreasing portal pressure, somatostatin and analogues have been used in acute variceal bleeding In this patient population, metaanalyses [29] have shown no benefit...

Ngày tải lên: 12/08/2014, 23:24

... cause and the cause of all women This, then, is the novelty in George Sand's way of presenting feminist grievances She had not invented these grievances They were already contained in Madame de ... leaving George Sand with Pagello in Venice The sentimental exaggeration continued, as we see from the letters exchanged between Musset and George Sand When crossing the Simplon the immutable grandeur ... comes back to Blanche, and makes her live over again her life as Denise In the mean time Rose had become a great singer She now arrives, just in time to be present at her friend's deathbed She enters...

Ngày tải lên: 06/03/2014, 23:21

... care better than the one who have less experience HA: Accountants who have less experience applied the code of professional competence and due care better than the one who have more experiences ... different research in difference province will give better result than the one province Therefore, they need to be have big size of research than now, and it may be have accurate result Secondly, ... have more experiences work more objectively than the one who have less experience Hypothesis 2: H0: Accountants who have more experiences apply the code of professional competence and due care...

Ngày tải lên: 13/03/2014, 14:20

... the presence of an amine substrate In the presence of xenon, plant enzymes form yellow TPQsq, whereas in pig enzyme the bleached species TPQaq is observed A lysine residue at the active site ... cadaverine and benzylamine These results are most compatible with two different mechanisms being involved in the interaction between enzyme and substrate It is possible, only in the plant enzyme, ... there is an interaction between the dissolved xenon and the interior of the protein However, such 129Xe NMR experiments cannot provide a more detailed characterization of the interaction between...

Ngày tải lên: 30/03/2014, 09:20

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Ngày tải lên: 24/04/2014, 17:07

... anthracycline-containing chemotherapy (e. g., IMEP [ifosfamide, etoposide, methotrexate, prednisone]) Therefore, 118 patients were included in the trial Medical records were retrospectively reviewed ... who were treated with anthracycline-containing chemotherapy as first-line treatment and excluded patients who did not receive any treatment or who received up-front ASCT Therefore, patients enrolled ... situations, inflammatory mediators seem to play an important role in the development and progression of cancers [26] There are some reports on the relationship between inflammation and cancer treatment...

Ngày tải lên: 10/08/2014, 21:23

... patient and terminated the pregnancy in the other SKS gave anesthesia to the patient and helped to collect the literature for this report SRS and SN wrote the article All the authors have read and ... approved the final manuscript Competing interests The authors declare that they have no competing interests Received: November 2009 Accepted: 26 May 2011 Published: 26 May 2011 References Del Brutto ... after two days She was followed-up regularly throughout the pregnancy The remainder of her antenatal period was uneventful but, at term, she had an emergency Cesarean section due to fetal distress...

Ngày tải lên: 11/08/2014, 00:23

... (occult) hypoperfusion related either to decreases in oxygen delivery or to increases in oxygen demand Competing interests None declared References From these studies it is clear that increased blood ... output) can normalize blood lactate levels in these patients Failure to normalize increased blood lactate levels with these interventions is generally associated with increased morbidity and mortality ... Available online http://ccforum.com/content/8/2/96 change intraoperatively, lactate levels increased Following surgery cardiac output increased, and those authors suggested that this represented physiological...

Ngày tải lên: 12/08/2014, 20:20