an enveloped virus with a helical nucleocapsid

... Page of Figure Binding of parental TK04-190Asp and mutant TK04190Ala viruses to plasma membrane preparations (PMP) from human, pig and turkey primary tracheal/bronchial epithelial cells Both viruses ... evaluated the replication of TK04 parental and HA-mutant viruses (hereafter referred as 190Asp and 190Ala, respectively) in human, pig and turkey primary tracheal/bronchial epithelial cells Virus ... included a wider range of turkey H3N2 TR viruses in the cross reactivity test Again, anti-190Asp antisera reacted better against most turkey viruses compared to anti-190Ala antisera (Table 1) For example,...

Ngày tải lên: 12/08/2014, 01:22

... 19-NA at various concentrations, the conductance of Art KSI/mA51-labeled SiNW-FET rapidly increased to a constant value [Fig 5(b)] 19-NA at a greater concentration resulted in a greater conductance, ... novel nano-bio-device can attain a femtomolar level Acknowledgements National Science Council and the MOE-ATU Program in Taiwan provided financial support References [1] B.G England, G.H Parsons, ... Chang et al / Sensors and Actuators B 138 (2009) 148–153 149 with HF solution After defining the contact pad patterns, a stack of Ti (10 nm) and Au (100 nm) was then evaporated with a thermal...

Ngày tải lên: 16/03/2014, 15:23

... Rahman S, Almutawa A ASB, Al-Bedah AM, Al-Rabeah AM, Ali Bahaj A, El-Awa F, CW JN, Asma S: Prevalence of tobacco use among students aged 13-15 years in Health Ministers’ Council/Gulf Cooperation ... Dar-Odeh NS, Bakri FG, Al-Omiri MK, Al-Mashni HM, Eimar HA, Khraisat AS, Abu-Hammad SM, Dudeen AA, Abdallah MN, Alkilani SM, Al-Shami L, AbuHammad OA: Narghile (water pipe) smoking among university ... 20 Nafae A, Misra SP, Dhar SN, Shah SN: Bronchogenic carcinoma in Kashmir Valley Indian J Chest Dis 1973, 15(4):285-295 21 Gunaid AA, Sumairi AA, Shidrawi RG, al-Hanaki A, al-Haimi M, al-Absi...

Ngày tải lên: 11/08/2014, 18:20

... efficiency against both antibiotic-susceptible and antibiotic-resistant bacterial strains Killing of bacteria by fowlicidins starts immediately on contact with bacteria, in sharp contrast with human cathelicidin ... hydrophobicity, and helicity are among the most important factors that influence the antibacterial and toxicity of a- helical cationic peptides [23,24], rational changes of these structural and physicochemical ... Gram-positive bacteria (Listeria monocytogenes ATCC 19115, Staph aureus ATCC 25923, Staph aureus ATCC BAA-39, and Staph aureus ATCC 43300) were purchased from either ATCC (Manassas, VA, USA) or MicroBiologics...

Ngày tải lên: 07/03/2014, 11:20

... the transmembrane region of APP, and has the typical amino acid composition of transmembrane helices, i.e small (Gly and Ala) and hydrophobic (Ile, Leu, Met and Val) residues [36] The only charged ... with the routine CALIBA of the program package DYANA [29] After discarding redundant and duplicated constraints, the final list included 130 intraresidue and 283 interresidue (149 sequential and ... concentration and temperature conditions Several organic solvents and mixtures of organic solvents with water, such as trifluoroethanol, trifluoroethanol/H2O, hexafluoroacetone hydrate, hexafluoroacetone...

Ngày tải lên: 08/03/2014, 09:20

... and transfected with calf thymus DNA, show DAPI staining of cellular nuclei and little background staining with anti-HA and anti-Myc antibodies (negative control) (B) DAPI staining of cellular ... DAPI staining of cellular nuclei and little background staining with anti-HA and anti-Myc antibodies (negative control) (B) DAPI staining of cellular nuclei and viral DNA Discrete areas of DNA ... Rockland Immunochemicals Inc.) Actin was detected and visualized using rabbit IgG anti-actin primary antibody (Cat # A5 060, Sigma-Aldrich) and goat anti-rabbit IgG (H&L) IRDye 800 conjugate secondary...

Ngày tải lên: 18/06/2014, 18:20

... and transfected with calf thymus DNA, show DAPI staining of cellular nuclei and little background staining with anti-HA and anti-Myc antibodies (negative control) (B) DAPI staining of cellular ... DAPI staining of cellular nuclei and little background staining with anti-HA and anti-Myc antibodies (negative control) (B) DAPI staining of cellular nuclei and viral DNA Discrete areas of DNA ... Rockland Immunochemicals Inc.) Actin was detected and visualized using rabbit IgG anti-actin primary antibody (Cat # A5 060, Sigma-Aldrich) and goat anti-rabbit IgG (H&L) IRDye 800 conjugate secondary...

Ngày tải lên: 20/06/2014, 01:20

... Turkish patients Abbreviations ACC: American College of Cardiology; AHA: American Heart Association; CABG: coronary artery binding graft; CK: creatine kinase; MACE: major adverse cardiac events; ... log-rank test A P value < 0.05 was considered statistically significant RESULTS Baseline clinical, coronary angiographic and lesion characteristics are shown in Table and Table No significant ... the ZES is safe and reduces the rates of clinical and angiographic restenosis in patients with symptomatic coronary artery disease (CAD; 4) Also the safety and efficacy of Paclitaxel-eluting...

Ngày tải lên: 25/10/2012, 11:18

... received the PES Baseline angiographic characteristics were also similar according to the modified ACC/AHA (American College of Cardiology/American Heart Association) classification.22 Overall, most ... 001 04 307 Abbreviations: CABG, Coronary artery bypass graft; MACE, Major adverse cardiac event (ie, death, myocardial infarction, and target vessel revascularization a Indicates patients who ... Turkish patients Acknowledgments All support for this study came from institutional and departmental resources Abbreviations ACC: American College of Cardiology; AHA: American Heart Association; CABG:...

Ngày tải lên: 26/10/2012, 08:57

... necessarily mean that translation starts from a downstream AUG, as predicted by genome and mRNA analysis, but raises the possibility that the translated form may have used a CUG start codon with an ... from an 83-year-old man and a 71.0 g sample of occipital cortex from a 85-year-old man with a short (25 h) post mortem delay RNA isolation, reverse transcription and 5Â-RACE Total RNA was isolated ... Isolation and chemical identication of trypsinogen from human brain Different antihuman trypsinogen mAbs were raised separately against recombinant human trypsin (mAb B1, mAb B7) and the 28-amino...

Ngày tải lên: 07/03/2014, 10:20

... potential to form an a- helix with a balance between amphiphilicity and hydrophobicity, and structural characteristics that are associated with obliqueorientated a- helices (Figs and 2) It can be ... Moffat, National Research Council, Ottawa, Ontario, Canada The band shapes of the single components are superpositions of Gaussian and Lorentzian band shapes Best ts were obtained by assuming a ... amphipathic alpha helical antimicrobial peptides Biopolymers 47, 451463 48 Tytler EM, Segrest JP, Epand RM, Nie SQ, Epand RF, Mishra VK, Venkatachalapathi YV & Anantharamaiah FEBS Journal 273...

Ngày tải lên: 07/03/2014, 12:20

... backbone w angle restraints were obtained from analysis of dNa/daN ratios [38] The w angle restraint was set to )30° ± 110° for dNa/ daN ratios less than 1, and to 120° ± 100° for dNa/daN ratios ... Engineering Research Council of Canada, the Alberta Heritage Foundation for Medical Research, CanBiocin Ltd (Edmonton, AB), and the Canada Research Chair in Bioorganic and Medicinal Chemistry References ... Isolation, purification and partial characterization of plantaricin 423, a bacteriocin produced by Lactobacillus plantarum J Appl Microbiol 84, 1131–1137 22 Jack, R.W., Wan, J., Gordon, J., Harmark,...

Ngày tải lên: 07/03/2014, 15:20

... in green algae and vascular plants, i.e the green lineage of plants ClpP genes are also found in Cyanobacteria [22] and in the genome of the Cyanophora cyanelle, an ancestral chloroplast In the ... ClpP1 antibody The ClpP1H and ClpP1L bands decrease slowly and concomitantly after 24 h, indicating that both are stable in the cell As a loading control, a duplicate blot was reacted with an antibody ... Institute and the Chlamydomonas Genome Project for EST data and ´ cDNA material, and H Moreau (Observatoire Oceanologique de Banyuls, France) for granting us access to the Ostreococcus blast analysis...

Ngày tải lên: 16/03/2014, 23:20

... and with a GAPDH probe (cf Fig 2) Relative background transcription was calculated from the ratios of signals for transporter mRNA and GAPDH mRNA With OCT1, OCT2, and CAT4 both vectors were analyzed ... graded transactivation potential Nucleic Acids Res 25, 2723–2729 13 Akagi K, Kanai M, Saya H, Kozu T & Berns A (2001) A novel tetracycline-dependent transactivator with E2F4 transcriptional activation ... were analyzed alongside on a single blot OCT, organic cation transporter; CAT, cationic amino acid transporter Transporter cDNA (human) Transporter mRNA ⁄ GAPDH mRNA Vector Doxycycline + Name Gene...

Ngày tải lên: 23/03/2014, 09:21

... believed that many membrane-active antimicrobial peptides pass through the peptidoglycan layer and then kill the target micro-organism by interacting with and permeabilizing the cytoplasmic membrane ... Membrane-active peptides mediate a wide range of biological events, including signal transduction, transport through the membrane, membrane fusion and lysis, ion channel formation, and antimicrobial ... concentrations exhibited an isodichroic point at 203 nm, indicating a two-state equilibrium between a random coil and an a- helix In pure water, M17 and M25 also became more a- helical as the NaCl...

Ngày tải lên: 23/03/2014, 10:21

... resonance of 4,4-dimethyl-4-silapentane-1-sulfonic acid used as an internal standard Distance restraints and structure calculations An initial survey of distance constraints was performed on a series ... zation of a novel conus peptide with apparent antinociceptive activity J Biol Chem 275, 32391–32397 18 Sharpe IA, Gehrmann J, Loughnan ML, Thomas L, Adams DA, Atkins A, Palant E, Craik DJ, Adams DJ, ... outcome was a set of 20 ˚ structures with a mean global rmsd of 0.56 ± 0.16 A and a ˚ mean global heavy atom rmsd of 1.30 ± 0.28 A Structural refinement was carried out using amber and structure quality...

Ngày tải lên: 30/03/2014, 09:20

... maser features and the simultaneous dimming of nearby features can be interpreted as originating from the passage of a magnetohydrodynamic (MHD) shock (Alakoz et al 2005) The shock was probably ... close to VLA2, at a distance of 55 mas (±40 mas), or at the projected distance of 110 AU (±80 AU) Therefore, the OH masers may well be located in the same shell as the water masers The magnetic ... This Zeeman pair was probably overlooked by the authors, or dismissed as showing too large a velocity separation d From EVN data are really new as they are related to the flare which took place between...

Ngày tải lên: 28/04/2014, 13:15

... by epitaxial mismatch with a substrate, will change phosphorene from a direct-gap to an indirect-gap semiconductor with a significantly smaller gap Details of the computational approach are listed ... transfer curves for drain bias values Vds = 0.01 and 0.5 V, which indicate a current on/ off ratio of ∼104, a very reasonable value for a material with a bulk band gap of 0.3 eV We also note that, ... Interest: The authors declare no competing financial interest 4040 2014 www.acsnano.org ARTICLE 36 Fang, H.; Chuang, S.; Chang, T C.; Takei, K.; Takahashi, T.; Javey, A High-Performance Single Layered...

Ngày tải lên: 06/05/2014, 08:54

... by epitaxial mismatch with a substrate, will change phosphorene from a direct-gap to an indirect-gap semiconductor with a significantly smaller gap Details of the computational approach are listed ... transfer curves for drain bias values Vds = 0.01 and 0.5 V, which indicate a current on/ off ratio of ∼104, a very reasonable value for a material with a bulk band gap of 0.3 eV We also note that, ... Interest: The authors declare no competing financial interest 4040 2014 www.acsnano.org ARTICLE 36 Fang, H.; Chuang, S.; Chang, T C.; Takei, K.; Takahashi, T.; Javey, A High-Performance Single Layered...

Ngày tải lên: 06/05/2014, 08:58

Ngày tải lên: 12/06/2014, 11:27