amplification of 34 rohu microsatellite loci in labeo bata and their transferability in six other species of the cyprinidae family
Báo cáo khoa hoc:"Microsatellite loci in Japanese quail and cross-species amplification in chicken and guinea fowl" pps
Ngày tải lên :
09/08/2014, 18:21
... determine the utility of markers isolated from other Phasianidae species in the chicken In a preliminary effort, we isolated 50 original quail microsatellite markers and found 46.0% of them to ... Cross -species amplification of Japanese quail markers in chicken and guinea fowl Table I also shows the results of cross -species amplification of all 100 quail markers in chicken and guinea fowl In ... were perfect repeats and (GUC0001 and GUC0010) were imperfect repeats as found in their corresponding quail loci For the remaining loci, the repeat array was either perfect in the chicken, as opposed...
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Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for amplification of mitochondrial apoptotic cell death in human cervical cancer cells pptx
Ngày tải lên :
07/03/2014, 05:20
... response of tumor cells However, the basis of the link between the signaling pathway and the apoptotic celldeath machinery in response to ionizing radiation remains largely unclear The aim of our investigation ... protein of the Bcl-2 family, in the presence of apoptotic stimuli The truncated Bid then triggers activation of Bax and ⁄ or Bak and mitochondrial release of pro-apototic molecules into the cytosol ... loop domain, including Ser70 and Ser87, is critically involved in the apoptotic process, and is induced by microtubule-damaging agents such as paclitaxel, docetaxel, vincristine and vinblastine [14]...
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Báo cáo khoa học: Cholinoceptive and cholinergic properties of cardiomyocytes involving an amplification mechanism for vagal efferent effects in sparsely innervated ventricular myocardium ppt
Ngày tải lên :
23/03/2014, 05:22
... suppressed remodeling of the myocardial infarction-induced failing heart despite the extreme sparseness of vagal nerve endings in the ventricle In other words, ACh released from vagal nerve endings sequentially ... Comparison of inhibitory activities of donepezil and other cholinesterase inhibitors on acetylcholinesterase and butyrylcholinesterase in vitro Methods Find Exp Clin Pharmacol 22, 609–613 28 Papandreou ... accordance with the guiding principles of the Physiological Society of Japan Materials and reagents ACh, the muscarinic receptor agonist pilocarpine and the muscarinic receptor antagonist atropine were...
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Báo cáo khoa học: Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp. BH72 ppt
Ngày tải lên :
07/03/2014, 02:20
... Fe-proteins or other proteins Results and Discussion Site-directed mutagenesis of the target arginine of dinitrogenase reductase An indication for the covalent modification of one subunit of dinitrogenase ... staining protocol; (b) a colloidal Coomassie staining solution [36]; (c) a zinc-imidazole stain [37]; and (d) a copper stain [38], as well as their impact on the further processing of proteins ... ADP-ribosylation, resulting in the observed migration difference during 2D gel electrophoresis Another striking difference of the MALDI-TOF spectra from the modified Fe-protein in comparison to the unmodified...
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Báo cáo khoa học: Hyperefficient PrPSc amplification of mouse-adapted BSE and scrapie strain by protein misfolding cyclic amplification technique pptx
Ngày tải lên :
23/03/2014, 04:21
... at the end of PrPSc polymers [4], indicating that the PrPSc accumulation rate is regulated by the number of polymers An increase in the number of PrPSc polymers is acquired mainly by breaking ... risk of contamination Furthermore, although mild amplification has also been demonstrated in other mammalian species, such as mice, cervids and humans, the amplification levels in these species are ... repeating the sonication, particularly in the presence of 0.05% digitonin (Fig 2B) By contrast, the amplification and final quantity of PrPSc were not affected by digitonin (Fig 2B,C), indicating...
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Báo cáo khoa học: Role of glutaredoxin 2 and cytosolic thioredoxins in cysteinyl-based redox modification of the 20S proteasome docx
Ngày tải lên :
23/03/2014, 07:20
... LC and HC, light and heavy chains of IgG immunoglobulin forms (cytosolic and mitochondrial matrix) [34] , as the same band was seen in the anti-ubiquitin blot (Fig 7, anti-Ub) Blotting of the ... four-stranded, mixed b-sheet and five a-helices The b-sheet forms the central core of the protein, with helices and located on one side of the sheet and helices 2, and located on the other side ... solventaccessible All of the other cysteine residues are either buried in the skeletal structure or exposed to the internal catalytic chamber environment Therefore, we investigated whether Grx2 enters the core...
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Báo cáo khoa học: Identification of the N-terminal region of TjZNT2, a Zrt⁄Irt-like protein family metal transporter, as a novel functional region involved in metal ion selectivity ppt
Ngày tải lên :
29/03/2014, 00:20
... with the exception of the N-terminus and the TM III–IV loop To examine the functions of TjZNT1 and TjZNT2, these transporters were tagged with hemagglutinin (HA) at their N-termini to verify their ... present in the sequence of TjZNT1 The present work provides several pieces of evidence for the involvement of this N-terminal region in the ion selectivity of TjZNT2 and related proteins First, ... This indicates that the N-terminal region does not inactivate TjZNT2, but affects the ion selectivity of the protein Finally, tagging with HA at the N-terminus of TjZNT1 was found to alter the...
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Tài liệu Báo cáo khoa học: Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx
Ngày tải lên :
16/02/2014, 15:20
... serine ⁄ threonine kinase that phosphorylates actin [12], binds the deubiquitinating enzyme otubain (OTUB1) [13,14], the small G protein subunit Gaq [15] and interacts with members of the Rho family ... addition to actin, other host cell proteins have been shown to bind to the virulence factor YpkA, including OTUB1 [13] In order to investigate the role of OTUB1 in Yersinia invasion of HEK293T cells, ... [2] and there are reports of emerging multidrug resistant strains [3] Pathogenic Yersiniae are typically taken up through ingestion and first reach the intestine The Yersinia surface protein invasin...
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
Ngày tải lên :
18/02/2014, 13:20
... handling using the devices and loss of peptide on the column resin The only detriment of the peptides produced here is the fact that they contain an exogenous N-terminal methionine However, the ... [40]; thus the presence of the N-terminal methionine may enable detection of Asp1 that would otherwise be invisible in 1H15NHSQC spectra Therefore, the presence of the N-terminal methionine will ... 8.4, and on SDS-PAGE the major band migrated between and kDa (Fig 2) These data indicate that both peptides accumulate in inclusion bodies, and that Ab(L1–40) is the dominant protein in the inclusion...
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Tài liệu Báo cáo khoa học: Proteasome involvement in the degradation of the Gq family of Ga subunits pptx
Ngày tải lên :
20/02/2014, 03:20
... in favor of the need of other proteins to stabilize the proteins in the membrane The interaction of Ga subunits with Gbc could be a stabilizing factor Mutations on Gaq residues which locate in ... ligand produces a decrease in G protein levels [21] On the other hand, our results have provided evidence for the lack of ligand-induced degradation of Gaq proteins Neither receptor-activation of ... enhancement in the rate of degradation compared with the wild-type protein Our data is more consistent with an increased destabilization of the protein in the cytoplasm, a process that, in the case of the...
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Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
Ngày tải lên :
21/02/2014, 03:20
... shown in Fig 8, the introduction of mutation into one of the Ets-binding sequences at )133 (GGAA to GTAA) greatly decreased the promoter activity, whereas mutations in the other Ets-binding site ... cells and their malignant behavior The increased expression of a3b1 integrin in gastric carcinoma cells is associated with their increased invasion and metastatic potentials [24,28] Thus, the transcriptional ... binding sequences for several transcription factors including Ets, GATA, and MyoD/E-box binding factors The introduction of mutation into one of the putative Etsbinding sequences suppressed the...
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Báo cáo khoa học: Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx
Ngày tải lên :
07/03/2014, 00:20
... increasing the bioavailability of IGFs by enhancing the proteolytic cleavage as well as for producing the C-terminal domain We have examined the amino acid sequences of the other protein family ... and in the liver increases under hypoxic conditions in the uterus, resulting in intrauterine growth retardation [15,16] Its mature polypeptide chain consists of 234 amino acids and, as all other ... together with identical aliquots of the untreated proteins (Fig 9A) The degradation process was evaluated on the basis of the ratio between the integrated areas of the intact protein in the treated...
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Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
Ngày tải lên :
07/03/2014, 00:20
... that the failure of these seven mutations to abolish protein phosphorylation was a result of other protein kinases masking the PKA-catalysed part Indeed, protein kinases other than PKA, including ... GIRK1 subunit was a prominent target for PKA in vitro, after the in vivo treatment of the oocytes with a PKA inhibitor and also in untreated oocytes On the other hand, the in vitro phosphorylation ... demonstrate that the GIRK1 subunit serves as PKA substrate both in vitro and in vivo This is in line with observations obtained in another study demonstrating in vitro PKA phosphorylation of the GIRK1...
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Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx
Ngày tải lên :
08/03/2014, 16:20
... 1) On the other hand, in our study, the full length of homologs of ScRpt4 and ScRpt6 were cloned by 5¢ RACE using C50126 and R1494 as primers from the rice shoot cDNA library to determine their ... contained in bands and showed no amino-acid sequence similarity to the RP subunits, suggesting that these proteins may be novel subunits of rice RP or proteins interacting with rice RP In the ESI-Q-TOF ... identified in the rice proteasome Processing of proteins is important for their function In the 20S proteasome, processing during assembly produces the active Thr residue at the N-termini of three...
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Báo cáo khoa học: Sequences and structural organization of phospholipase A2 genes from Vipera aspis aspis, V. aspis zinnikeri and Vipera berus berus venom Identification of the origin of a new viper population based on ammodytin I1 heterogeneity docx
Ngày tải lên :
17/03/2014, 03:20
... affecting the toxicity of the protein This is probably the case for changes in the residues involved in the interaction between the two subunits: Asn1Ser, Phe3Leu in the PLA2 subunit and Gln34His in ... PLA2 The signal peptides of all the proteins were 16 amino acids long The mature proteins consisted of 122 amino acids for ammodytin I1, the A and B chains of vaspin and V b berus PLA2, and 121 ... genome of the neurotoxic V a aspis, with six of the 10 sequenced clones having the 126 bp deletion as in V a aspis, V a zinnikeri, and the remaining four clones having an intron D similar to that of...
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Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx
Ngày tải lên :
17/03/2014, 10:20
... polypeptides, all the proteins of interest were present in the soluble fraction of the induced cell in the case of the expression of pET22b(+)/touBEA and /touC, whereas the product of the expression of pET22b(+)/touF ... turnover assays of the individual components (10 nmol of Tomo H, 20 nmol of Tomo C and Tomo D subunits) and of each of their possible combinations, were performed by adding the proteins to reaction ... the anticipated sequence of subunit C on the basis of their mass value and the specificity of the enzyme, leading to the complete verification of the amino acid sequence of subunit C (GenBank accession...
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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx
Ngày tải lên :
23/03/2014, 03:20
... Two Gly (216 and 226, chymotrypsin numbering) are located on the wall of the binding pocket and allow the access of bulky residues, like Arg and Lys, whereas the basic side chain of these residues ... occurs in all other clones PaTry4 was the largest and the least anionic of all trypsins found in P argus No cationic form of the enzyme was found in this work The coding regions of PaTry1a and PaTry1b ... numbering) is involved in the formation of a salt bridge with the N-terminal Ile of the mature enzyme for completing the formation of Fig Distribution of charged amino acids in Panulirus argus and...
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Báo cáo khoa học: Identification of the heparin-binding domains of the interferon-induced protein kinase, PKR pptx
Ngày tải lên :
23/03/2014, 13:20
... to the dsRNA-binding domain of PKR, its heparin-binding domains are located in the carboxy-terminal half of the molecule The aminoterminal heparin-binding domain (ATD) overlaps with the ATP-binding ... heparin-binding and ATP-binding sites Our results indicate that the ATP-binding residues and heparin-binding residues are present within the aminoterminal heparin-binding domain (ATD) of PKR Thus, in contrast ... Heparin-binding domains of PKR Fig (A) Schematic representation of the heparin-binding domains of PKR The relative positions of the domains involved in PKR binding to different activators are indicated...
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Báo cáo Y học: Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides pot
Ngày tải lên :
23/03/2014, 21:20
... nondenaturing conditions and revealed one main band and two weaker bands Their dissociation by SDS in the second dimension showed that the main band was released into three subunits migrating at the ... analysis of the soluble and insoluble fractions was performed (Fig 1B) The subunits of the diol dehydratase, mainly the medium subunit, were present in the insoluble fraction suggesting that the recombinant ... subunits of the protein was controlled by SDS/PAGE (Fig 1A) Despite the high level of synthesis of the three proteins at 61, 24.7 and 19.1 kDa, Fig SDS/PAGE of cell-free extracts of E coli showing the...
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Báo cáo khoa học: Classification of the short-chain dehydrogenase ⁄reductase superfamily using hidden Markov models potx
Ngày tải lên :
29/03/2014, 09:20
... functions in steroid hormone, prostaglandin and retinoid metabolism, and hence signaling They also play crucial roles in the metabolism of xenobiotics, including drugs and carcinogens A growing number ... 900 proteins, and surprisingly, given that the majority of the families are classical, 36% of the proteins are of the extended type This means that many of the largest families are of the extended ... SDRs In every family except two, all of the members have the largest number of identities with the human representatives in their own family The first exception is the retinol dehydrogenase family...
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