amp of amino acid nitrogen

Tài liệu THE ROLES OF AMINO ACID CHELATES IN ANIMAL NUTRITION pdf

Ngày tải lên : 15/02/2014, 16:20

... Association of Feed Control Officials, a metal amino acid chelate is lithe product resulting from the reaction of a metal ion from a soluble salt with amino acids with a mole ratio of one mole of metal ... 47 76 xx Contents EVALUATION OF THE NUTRITIONAL EFFICIENCY OF AMINO ACID CHELATES 86 Silvano Maletto and Germano Cagliero AN ASSESSMENT OF LONG TERM FEEDING OF AMINO ACID CHELATES 106 Robert B ... of a specific mineral in the feed; (b) The Roles of Amino Acid Chelates in Animal Nutrition an imbalance of another mineral or nutrient including certain vitamins, amino acids and fats, any of...

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Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identiﬁcation of amino acid residues critical for eIF5A activity and hypusine modiﬁcation doc

Ngày tải lên : 16/03/2014, 06:20

... Analysis of the position of both amino acids in the human eIF5A-1 model, Leu91 and Leu101, shows that they are localized at the hydrophobic core of the b-barrel (Fig 1) Substitution of either of the ... or C-terminal domains of eIF5A are required for eIF5A activity, we generated truncated eIF5A eIF5A with deletions of six or 13 amino acids from the N-terminus, or ﬁve amino acids from the C-terminus, ... to 30% of the initial rate of UBHY-R, whereas the rates of synthesis of DNA and RNA were 70% and 60% of the initial values, respectively These data suggest that the primary effect of eIF5A...

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Báo cáo khoa học: Weak organic acid stress inhibits aromatic amino acid uptake by yeast, causing a strong inﬂuence of amino acid auxotrophies on the phenotypes of membrane transporter mutants ppt

Ngày tải lên : 23/03/2014, 21:20

... Defects in several of the enzymes of aromatic amino acid biosynthesis therefore hypersensitize yeast to weak organic acid stress We also investigated the growth of this same series of strains after ... aromatic amino acids, might be causing an unusually high sensitivity to weak organic acid stress Auxotrophic requirements for aromatic amino acids dramatically increase sensitivity to weak organic acid ... levels of sorbate or acetate indicated that the loss of Azr1p did not result in increased sensitivity to either of these acids (Fig 1; strains with no auxotrophic requirements for aromatic amino acids)...

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Báo cáo Y học: The effect of amino-acid substitutions I112P, D147E and K152N in CYP11B2 on the catalytic activities of the enzyme pdf

Ngày tải lên : 24/03/2014, 03:21

... investigated whether the amino acids of human CYP11B2 located in regions aligned with these helices are of functional importance They were replaced by the corresponding amino acids of human, mouse and ... cytochrome P450 [23], and as the conversion of a multitude of compounds might be due to the high variability in the SRS of the family P450s [35], amino acid 112 of CYP11B1 and CYP11B2 may therefore ... So far, the role of distinct amino acids of the N-terminal regions of human CYP11B isozymes has not been studied extensively, although it is known that the N-terminal domains of CYP11B1 and CYP11B2...

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báo cáo khoa học: " Identification of amino acid residues involved in substrate specificity of plant acyl-ACP thioesterases using a bioinformatics-guided approach" pptx

Ngày tải lên : 12/08/2014, 05:20

... analysis Fatty acid content of the medium from various cell cultures was determined by the production and measurement of fatty acid methyl esters Briefly, 22 μl of glacial acetic acid and ml of 1:1 (vol:vol) ... the family where there is conservation of sequence and positions where there is conservation of amino acid properties within one of the classes (for example, see Figure 3) At nine positions, ... GAEVVRGRTEWSSKTPTTTWGTAP * Figureacid sequence alignment of Arabidopsis thaliana FatA3 (NP_189147) with FatB3-2 (CAA85388) Amino Amino acid sequence alignment of Arabidopsis thaliana FatA3 (NP_189147)...

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Báo cáo y học: " Conversion of amino-acid sequence in proteins to classical music: search for auditory patterns" doc

Ngày tải lên : 14/08/2014, 07:21

... version of this paper Additional data file is a music clip of the human ThyA protein based on the single note assignment of one amino acid per musical note Additional data file is a music clip of ... portion of this sequence fixed to a 20-note range (2.5 octaves), where each amino acid was initially assigned to a unique note One way to improve the musicality is to express each amino acid as ... reduced-note assignment Certain similar amino acids were paired and assigned a three-note chord (triad) starting an octave below middle C Each member of the amino- acid pair was distinguished from the...

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Báo cáo y học: " The hidden universal distribution of amino acid biosynthetic networks: a genomic perspective on their origins and evolution" pptx

Ngày tải lên : 14/08/2014, 08:21

... the evolution of amino acid biosynthesis in diverse species Results and discussion Biological distribution of amino acid biosynthetic networks The origins and evolution of amino acid biosynthesis ... taxonomic distribution Figure Taxonomic distribution of amino acid biosynthesis Taxonomic distribution of amino acid biosynthesis Conservation of amino acid biosynthesis from the E coli-centric and ... synthesize both amino acids Chorismate Chorismate is not an amino acid itself, but it is a key compound in the biosynthesis of aromatic amino acids and we consider the distribution of their catalyzing...

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Báo cáo y học: "Mutation patterns of amino acid tandem repeats in the human proteome" ppt

Ngày tải lên : 14/08/2014, 16:21

... contain expansions or contractions of the amino acid repeat We analyze the properties of repeats formed by different types of amino acids and identify a group of human genes that could potentially ... Figure Number 1of polymorphic variants for regions containing different kinds of amino acid repeats Number of polymorphic variants for regions containing different kinds of amino acid repeats For ... analysis of the types of amino acid substitutions, as well as the frequencies of synonymous and non-synonymous substitutions, in the two types of regions We have found that the vast majority of amino...

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Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural speciﬁcity variants doc

Ngày tải lên : 18/02/2014, 13:20

... [14C]-labelled amino acids or [32P]-labelled tRNA Assay method Aminoacylation of transcript tRNA with [14C]-labelled amino acid Aminoacylation of [32P]-labelled transcript tRNA Arg Arg Cav Cav Source of ... comparison of amino acid utilization by the plant arginyl-tRNA synthetases The aminoacylation level attained in the presence of L-arginine was compared to that in the presence of mM of the analogue ... be aminoacylated with arginine to a level of approximately 0.05 pmol amino acid ⁄ pmol tRNA The corresponding soybean transcript had an arginine acceptance level of approximately 0.1 pmol amino...

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Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt

Ngày tải lên : 18/02/2014, 13:20

... possible evolutionary events leading to separation of the heavy-chain subunit of these amino acid transporters from the enzymes involved in the metabolism of starch and related saccharides Results and ... genetics of heteromeric amino acid transporters Physiology 20, 112–124 MacGregor EA, Janecek S & Svensson B (2001) Relationship of sequence and structure to speciﬁcity in the a-amylase family of enzymes ... Heteromeric amino acid transporters: biochemistry, genetics, and physiology Am J Physiol Renal Physiol 281, 995–1018 Broer S & Wagner CA (2002) Structure–function relationships of heterodimeric amino acid...

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Tài liệu Báo cáo khoa học: Modulation of the enzymatic efﬁciency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx

Ngày tải lên : 18/02/2014, 17:20

... proline, aspartic acid, serine or alanine Simple and double FNR mutants of amino acids C266 and L268 were obtained and characterized It was observed that alteration of the amino acid volume decreases ... efficiencies of wild-type and mutant FNRs plotted as a function of different amino acid physicochemical properties The catalytic efficiencies of wild-type and mutant FNRs from Table (percentage of the ... nicotinamide of NADP+ is precisely tuned by selecting amino acids that face the other side of the tyrosine phenol ring The specific volumes of the above-mentioned residues condition the arrangement of Y308...

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Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

Ngày tải lên : 18/02/2014, 17:20

... of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues It is quite rare for such a variety of ... structure of conomarphin was similar to those of other conotoxin cDNAs The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 residues, a proregion of ... physiological role of speciﬁc Pro hydroxylation in conotoxins is still elusive 1982 The epimerized d-Phe13 is another striking feature of conomarphin The l -amino acid to d -amino acid epimerization...

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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc

Ngày tải lên : 18/02/2014, 18:20

... for individual amino acid contents are of the same order of magnitude as those of the total amino acid contents for glutamine (C), glutamic acid (D), asparagine (E), aspartic acid (F), alanine ... the synthesis and deamination of glutamate (Fig 3F) In vitro activities of nitrogen assimilation into amino acids The in vitro activities of the key enzymes of nitrogen assimilation were determined ... and amino acid) and Asx (asparagine and aspartate: four carbon amide and amino acid) were found to be the main nitrogen compounds in the phloem exudates, amounting to 51% of the total amino acids...

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Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

Ngày tải lên : 19/02/2014, 12:20

... the second-half reaction and are part of the active-site of CoaB; these residues may be directly involved in binding of one of the substrates, the amino acid cysteine (Fig 3) However, it cannot ... conditional lethality of the dfp-707 mutant is that one amino acid residue of the N-terminal PPC decarboxylase (CoaC) domain of Dfp is exchanged [4] For further characterization of the dfp-1 mutation, ... 4¢-phosphopantothenic acid, CTP and cysteine The synthesis of PPC occurs in two half reactions starting with the formation of 4¢-phosphopantothenoyl-CMP (activation of the carboxyl group of 4¢-phosphopantothenate)...

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Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt

Ngày tải lên : 19/02/2014, 16:20

... respectively) with those of the PP3591 gene of P putida KT2440 Results and Discussion Purication and subunit structure of NMAADH Identication of a gene encoding N-methyl-Lamino acid dehydrogenase (NMAADH) ... carrying pDPKA with a yield of 23% The N-terminal aminoacid sequence of the puried enzyme was identical with FEBS Journal 272 (2005) 11171123 ê 2005 FEBS N-Methyl-L -amino acid dehydrogenase from ... the presence of NADPH with N-methylalanine dehydrogenase (EC 1.4.1.17) [17] Therefore, in contrast with the N-methyl groups of depsipeptides and pilins, those of free N-methyl amino acids are derived...

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Tài liệu Báo cáo khoa học: Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

Ngày tải lên : 20/02/2014, 01:20

... concentration of mgÆmL)1 Samples were ﬁrst sonicated for 15 Then mL buffer or sample was loaded into a clean reference or sample cell, respectively, ensuring that the samples were free of air bubbles Samples ... and the role of key acidic amino acid residues in staphylococcal nuclease unfolding FEBS Journal 272, 3967–3974 Chen HM, Markin VS & Tsong TY (1992) pH induced folding ⁄ unfolding of staphylococcal ... the secondary structure of the protein disintegrated Using the peak at 222 nm of the wild-type protein as an index of helical conformational stability, this CD spectrum of W140O suggests a decrease...

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Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx

Ngày tải lên : 20/02/2014, 23:20

... sequence of the open reading frame (ORF) of their cDNAs and the corresponding aa sequences, in Table Summary of the purification of DNases I from the pancreases of three species of snake The results of ... Comparison of the three primary structures of deoxyribonuclease isolated from bovine, ovine, and porcine pancreas Derivation of the amino acid sequence of ovine DNase and revision of the previously ... presence of DNase I-specific mRNA was verified by RT-PCR amplification of the total RNA extracted from each snake tissue using sets of primers corresponding to the N- and C-terminal aa sequences of the...

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Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf

Ngày tải lên : 22/02/2014, 07:20

... network involves the side chains of the amino acids in positions 53 and 56 in a helix B, 186 in b strand 3A, and position 334 in b strand 5A (Fig 1; the numbering of amino acids in PAI-1 is according ... most of the BSA and most of the excess of LMW-uPA The samples were then precipitated with trichloroacetic acid and subjected to SDS/PAGE in gradient gels with 6–16% polyacrylamide The positions of ... combination of amino acids in these positions resulted in an accelerated latency transition Substitution of the Lys residue in position 335 counteracted the accelerating effect of some of the substitutions...

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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

Ngày tải lên : 22/02/2014, 07:20

... One unit of isomerase activity is defined as the amount of enzyme that produces mmol of product per under the assay conditions Activation of apo-BLXI by metals To examine the effect of bivalent ... the presence of Co2þ or Mn2þ are interesting With a similar inactivation mechanism, Ea of inactivation of T thermosulfurigenes XI is only 490 kJ·mol21 in the presence of Table Effect of metals on ... stability and lower Ea of inactivation of BLXI in the presence of Mg2þ reflect the fact that the enzyme binds Mg2þ with lower specificity (the Kact of Mg2þ is 10 times higher that that of Co2þ or Mn2þ...

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Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several speciﬁc biological processes through GCN2-dependent and GCN2-independent pathways ppt

Ngày tải lên : 07/03/2014, 03:20

... concentration of amino acids In addition, yeast cells possess an amino acid sensing system, localized at the plasma membrane, that transduces information regarding the presence of extracellular amino acids ... whereby a subset of genes is coordinately induced by starvation of the cell for one single amino acid [11] In mammals, our knowledge of the regulation of gene expression by amino acid availability ... ratio of the distribution of the amino acid- regulated genes* versus the distribution of the genes spotted onto the microarray** *Percentage of the representation of one GO term among all the amino...

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